LOCUS       sequence-470258-        9532 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for inducible expression of the green fluorescent 
            protein EGFP (TRE3G promoter).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9532)
  TITLE     Morsut lab plasmids 2024
REFERENCE   2  (bases 1 to 9532)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pHR_TRE3G_EGFP_WPRE
FEATURES             Location/Qualifiers
     source          1..9532
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    137..271
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(174..193)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     228..247
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             439..1072
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1119..1244
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1741..1974
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2159..2203
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2488..2605
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2667..3016
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, optimized for 
                     retroviral and lentiviral vectors"
     protein_bind    2675..2693
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2711..2729
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2747..2765
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2783..2801
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2819..2837
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2855..2873
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2891..2909
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2955..2979
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(3022..3024,3025..3027,3028..3738)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    3834..4422
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3887..3907)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4305..4316)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4425..4441)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4426..4442)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             4532..4765
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(4790..4808)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4791..4808)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(5098..5117)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(5331..5350)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5450..5472
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5510..5528)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5596..5700
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5701..5769,5770..6561)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5919..5938)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6732..7320
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7221..7240
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7474..7491
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7561..7581)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7566..7895
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7746..7881
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          9029..9094
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             9224..9244
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gaatatttga tgtatagtgc cttgactaga gatcataatc agccatacca catttgtaga
       61 ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa
      121 tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag
      181 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
      241 actcatcaat gtatcttatc atgtctggat caactggata actcaagcta accaaaatca
      301 tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt ttcatttact
      361 ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt gttaaatatg
      421 tactacaaac ttagtagttg gaagggctaa ttcactccca aagaagacaa gatatccttg
      481 atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac acaccagggc
      541 caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca gttgagccag
      601 ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct gtgagcctgc
      661 atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc cgcctagcat
      721 ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga tatcgagctt
      781 gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc gggactgggg
      841 agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac tgggtctctc
      901 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      961 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     1021 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
     1081 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
     1141 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
     1201 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
     1261 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
     1321 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
     1381 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
     1441 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
     1501 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
     1561 gtaagaccac cgcacagcaa gcggccggcc gctgatcttc agacctggag gaggagatat
     1621 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     1681 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     1741 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     1801 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     1861 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     1921 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     1981 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     2041 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     2101 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     2161 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     2221 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     2281 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     2341 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga
     2401 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta
     2461 tcgccaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
     2521 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
     2581 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccagttta
     2641 tcgatgaggc cctttcgtct tcactcgagt ttactcccta tcagtgatag agaacgtatg
     2701 aagagtttac tccctatcag tgatagagaa cgtatgcaga ctttactccc tatcagtgat
     2761 agagaacgta taaggagttt actccctatc agtgatagag aacgtatgac cagtttactc
     2821 cctatcagtg atagagaacg tatctacagt ttactcccta tcagtgatag agaacgtata
     2881 tccagtttac tccctatcag tgatagagaa cgtatgtcga ggtaggcgtg tacggtgggc
     2941 gcctataaaa gcagagctcg tttagtgaac cgtcagatcg cctggagcaa ttccacaaca
     3001 cttttgtctt atacttacgc gatggtttct aagggtgaag aattgttcac cggagttgtg
     3061 cccatcctgg ttgagctgga cggagacgtt aacggacaca agttctcagt gagcggcgaa
     3121 ggagagggcg acgctacgta cggtaaactg acgttgaagt tcatctgtac aacaggaaag
     3181 ctgccagtgc cctggcctac actcgtgacc accctcacct acggagtcca gtgcttctcc
     3241 cggtaccccg accacatgaa acagcacgat ttcttcaagt ccgcgatgcc tgagggctat
     3301 gttcaggaac gcaccatatt cttcaaggac gacggtaact ataaaaccag ggctgaagtt
     3361 aagttcgagg gggacacgtt ggtaaataga attgagttga aaggaatcga tttcaaagaa
     3421 gacggcaata ttttgggcca caagctggag tacaactaca acagccacaa cgtgtatatt
     3481 atggctgata agcagaagaa cggcatcaag gtgaacttca aaatccgaca caatatcgag
     3541 gatggctctg tgcaactggc cgaccattac cagcaaaaca cgcctatcgg cgacggtccg
     3601 gttttgctgc cagataacca ctacctctcc acgcaatcag ccctctccaa ggatcctaac
     3661 gaaaaaagag accatatggt tctgcttgaa ttcgtgacgg ctgctgggat caccctggga
     3721 atggatgagc tgtataaggg ccgcaactcc cacctgcaac atgcgtgact gactgaggcc
     3781 gcgactctag agtcgacctg caggcatgca agcttgatat caagcttatc gataatcaac
     3841 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     3901 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     3961 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     4021 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     4081 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     4141 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     4201 ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg
     4261 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     4321 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc
     4381 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgacctc
     4441 gagggaatta attcgagctc ggtaccttta agaccaatga cttacaaggc agctgtagat
     4501 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     4561 caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     4621 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     4681 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     4741 tagtcagtgt ggaaaatctc tagcagcatc tagaattaat tccgtgtatt ctatagtgtc
     4801 acctaaatcg tatgtgtatg atacataagg ttatgtatta attgtagccg cgttctaacg
     4861 acaatatgta caagcctaat tgtgtagcat ctggcttact gaagcagacc ctatcatctc
     4921 tctcgtaaac tgccgtcaga gtcggtttgg ttggacgaac cttctgagtt tctggtaacg
     4981 ccgtcccgca cccggaaatg gtcagcgaac caatcagcag ggtcatcgct agccagatcc
     5041 tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct
     5101 atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt
     5161 gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct
     5221 tgcatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct
     5281 tcctaatgca ggagtcgcat aagggagagc gtcgaatggt gcactctcag tacaatctgc
     5341 tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga
     5401 cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc
     5461 atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata
     5521 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     5581 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     5641 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     5701 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     5761 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     5821 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     5881 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     5941 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     6001 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     6061 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     6121 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     6181 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     6241 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     6301 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     6361 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     6421 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     6481 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     6541 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     6601 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     6661 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     6721 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     6781 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     6841 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     6901 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     6961 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     7021 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     7081 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     7141 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     7201 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     7261 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     7321 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     7381 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     7441 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     7501 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgt
     7561 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
     7621 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
     7681 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
     7741 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
     7801 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
     7861 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct tggacacaag acaggcttgc
     7921 gagatatgtt tgagaatacc actttatccc gcgtcaggga gaggcagtgc gtaaaaagac
     7981 gcggactcat gtgaaatact ggtttttagt gcgccagatc tctataatct cgcgcaacct
     8041 attttcccct cgaacacttt ttaagccgta gataaacagg ctgggacact tcacatgagc
     8101 gaaaaataca tcgtcacctg ggacatgttg cagatccatg cacgtaaact cgcaagccga
     8161 ctgatgcctt ctgaacaatg gaaaggcatt attgccgtaa gccgtggcgg tctgtaccgg
     8221 gtgcgttact ggcgcgtgaa ctgggtattc gtcatgtcga taccgtttgt atttccagct
     8281 acgatcacga caaccagcgc gagcttaaag tgctgaaacg cgcagaaggc gatggcgaag
     8341 gcttcatcgt tattgatgac ctggtggata ccggtggtac tgcggttgcg attcgtgaaa
     8401 tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc ggctggtcgt ccgctggttg
     8461 atgactatgt tgttgatatc ccgcaagata cctggattga acagccgtgg gatatgggcg
     8521 tcgtattcgt cccgccaatc tccggtcgct aatcttttca acgcctggca ctgccgggcg
     8581 ttgttctttt taacttcagg cgggttacaa tagtttccag taagtattct ggaggctgca
     8641 tccatgacac aggcaaacct gagcgaaacc ctgttcaaac cccgctttaa acatcctgaa
     8701 acctcgacgc tagtccgccg ctttaatcac ggcgcacaac cgcctgtgca gtcggccctt
     8761 gatggtaaaa ccatccctca ctggtatcgc atgattaacc gtctgatgtg gatctggcgc
     8821 ggcattgacc cacgcgaaat cctcgacgtc caggcacgta ttgtgatgag cgatgccgaa
     8881 cgtaccgacg atgatttata cgatacggtg attggctacc gtggcggcaa ctggatttat
     8941 gagtgggccc cggatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa
     9001 actacctaca gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt
     9061 taaactactg attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg
     9121 ggagcagtgg tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc
     9181 tagtgatgat gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa
     9241 ggtagaagac cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt
     9301 tagtaataga actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct
     9361 atacaagaaa attatggaaa aatattctgt aacctttata agtaggcata acagttataa
     9421 tcataacata ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta
     9481 tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa ggggttaata ag
//
