LOCUS       sequence-471068-       21494 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression TadDE in rice cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 21494)
  AUTHORS   Fan T, Cheng Y, Wu Y, Liu S, Tang X, He Y, Liao S, Zheng X, Zhang T,
            Qi Y, Zhang Y
  TITLE     High performance TadA-8e derived cytosine and dual base editors with
            undetectable off-target effects in plants.
  JOURNAL   Nat Commun. 2024 Jun 14;15(1):5103. doi: 10.1038/s41467-024-49473-w.
  PUBMED    38877035
REFERENCE   2  (bases 1 to 21494)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-06-14"; volume: "15(1):5103. doi"; pages: " 
            10.1038/s41467-024-49473-w"
COMMENT     Sequence Label: pGEL853
FEATURES             Location/Qualifiers
     source          1..21494
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     489..509
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             619..1326
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     1032..1051
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             1348..2373
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     CDS             1348..2373
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    2414..2588
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    2666..2690
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             3115..3909
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      5334..5922
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5823..5842
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6076..6093
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6108..6248
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6174..6193)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6334..6356
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      6592..6786
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(6852..7925)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(8354..8983)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    10284..10308
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     10511..10527
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    11103..11124
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11139..11144,11145..11162,11163..11169)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11177..11193
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11201..11217
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             11213..11620
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGARLQGG
                     TRQCIARCMHCLHCGHAATFCNLHKLACVQL"
     primer_bind     11236..11256
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11238..11256
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(11303..11322)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(11304..11322)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(11332..11348)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     terminator      complement(13270..13518)
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     CDS             complement(13534..13554)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(13597..13845)
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             complement(13876..14124)
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             complement(14161..14208)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             complement(join(14209..18282,18283..18285,18286..18309))
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             14288..14296
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(18334..18354)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(18955..18975)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        complement(19021..21018)
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     primer_bind     complement(21065..21081)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    21089..21105
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(21113..21119,21120..21137,21138..21143))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    21158..21179
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(21370..21494,1..552)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
ORIGIN
        1 aaggaaaggc tatcgttcaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac
       61 ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt
      121 gatgtgaaca tggtggagca cgacactctc gtctactcca agaatatcaa agatacagtc
      181 tcagaagacc aaagggctat tgagactttt caacaaaggg taatatcggg aaacctcctc
      241 ggattccatt gcccagctat ctgtcacttc atcaaaagga cagtagaaaa ggaaggtggc
      301 acctacaaat gccatcattg cgataaagga aaggctatcg ttcaagatgc ctctgccgac
      361 agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga agacgttcca
      421 accacgtctt caaagcaagt ggattgatgt gatatctcca ctgacgtaag ggatgacgca
      481 caatcccact atccttcgca agacccttcc tctatataag gaagttcatt tcatttggag
      541 aggacacgct gaaatcacca gtctctctct acaaatctat ctctctcgag ctttcgcaga
      601 tccggggggc aatgagatat ggtgagcaag ggcgaggagg ataacatggc catcatcaag
      661 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
      721 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
      781 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc
      841 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag
      901 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
      961 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc
     1021 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg
     1081 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac
     1141 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
     1201 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc
     1261 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctt
     1321 tacaagtcgg gcggaggtgg ctctgatatg aaaaagcctg aactcaccgc gacgtctgtc
     1381 gagaagtttc tgatcgaaaa gttcgacagc gtgtccgacc tgatgcagct ctcggagggc
     1441 gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat
     1501 agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc atcggccgcg
     1561 ctcccgattc cggaagtgct tgacattggg gaattcagcg agagcctgac ctattgcatc
     1621 tcccgccgtg cacagggtgt cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt
     1681 ctgcagccgg tcgcggaggc catggatgcg atcgctgcgg ccgatcttag ccagacgagc
     1741 gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg tgatttcata
     1801 tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga caccgtcagt
     1861 gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg ccccgaagtc
     1921 cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa tggccgcata
     1981 acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga ggtcgccaac
     2041 atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta cttcgagcgg
     2101 aggcatccgg agcttgcagg atcgccacga ctccgggcgt atatgctccg cattggtctt
     2161 gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg ggcgcagggt
     2221 cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc
     2281 agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga
     2341 cgccccagca ctcgtccgag ggcaaagaaa tagagtagat gccgaccggg atctgtcgat
     2401 cgacaagctc gagtttctcc ataataatgt gtgagtagtt cccagataag ggaattaggg
     2461 ttcctatagg gtttcgctca tgtgttgagc atataagaaa cccttagtat gtatttgtat
     2521 ttgtaaaata cttctatcaa taaaatttct aattcctaaa accaaaatcc agtactaaaa
     2581 tccagatccc ccgaattaat tcggcgttaa ttcagtacat taaaaacgtc cgcaatgtgt
     2641 tattaagttg tctaagcgtc aatttgttta caccacaata tatcctgcca ccagccagcc
     2701 aacagctccc cgaccggcag ctcggcacaa aatcaccact cgatacaggc agcccatcag
     2761 tccgggacgg cgtcagcggg agagccgttg taaggcggca gactttgctc atgttaccga
     2821 tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga tctcgcggag
     2881 ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatcacc gcggtttcaa
     2941 aatcggctcc gtcgatacta tgttatacgc caactttgaa aacaactttg aaaaagctgt
     3001 tttctggtat ttaaggtttt agaatgcaag gaacagtgaa ttggagttcg tcttgttata
     3061 attagcttct tggggtatct ttaaatactg tagaaaagag gaaggaaata ataaatggct
     3121 aaaatgagaa tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg cgtaaaagat
     3181 acggaaggaa tgtctcctgc taaggtatat aagctggtgg gagaaaatga aaacctatat
     3241 ttaaaaatga cggacagccg gtataaaggg accacctatg atgtggaacg ggaaaaggac
     3301 atgatgctat ggctggaagg aaagctgcct gttccaaagg tcctgcactt tgaacggcat
     3361 gatggctgga gcaatctgct catgagtgag gccgatggcg tcctttgctc ggaagagtat
     3421 gaagatgaac aaagccctga aaagattatc gagctgtatg cggagtgcat caggctcttt
     3481 cactccatcg acatatcgga ttgtccctat acgaatagct tagacagccg cttagccgaa
     3541 ttggattact tactgaataa cgatctggcc gatgtggatt gcgaaaactg ggaagaagac
     3601 actccattta aagatccgcg cgagctgtat gattttttaa agacggaaaa gcccgaagag
     3661 gaacttgtct tttcccacgg cgacctggga gacagcaaca tctttgtgaa agatggcaaa
     3721 gtaagtggct ttattgatct tgggagaagc ggcagggcgg acaagtggta tgacattgcc
     3781 ttctgcgtcc ggtcgatcag ggaggatatc ggggaagaac agtatgtcga gctatttttt
     3841 gacttactgg ggatcaagcc tgattgggag aaaataaaat attatatttt actggatgaa
     3901 ttgttttagt acctagaatg catgaccaaa atcccttaac gtgagttttc gttccactga
     3961 gcgctgatga atcccctaat gatttttatc aaaatcatta agttaaggta gatacacatc
     4021 ttgtcatatg atcaaatggt ttcgccaaaa atcaataatc agacaacaaa atgtgcgaac
     4081 tcgatatttt acacgactct ctttaccaat tctgccccga attacactta aaacgactca
     4141 acagcttaac gttggcttgc cacgccttac ttgactgtaa aactctcact cttaccgaac
     4201 ttggccgtaa cctgccaacc aaagcgagaa caaaacataa catcaaacga atcgaccgat
     4261 tgttaggtaa tcgtcacctc cacaaagagc gactcgctgt ataccgttgg catgctagct
     4321 ttatctgttc gggcaatacg atgcccattg tacttgttga ctggtctgat atccgtgagc
     4381 aaaaacggct tatggtattg cgagcttcag tcgcactaca cggtcgttct gttactcttt
     4441 atgagaaagc gttcccgctt tcagagcaat gttcaaagaa agctcatgac caatttctag
     4501 ccgaccttgc gagcattcta ccgagtaaca ccacaccgct cattgtcagt gatgctggct
     4561 ttaaagtgcc atggtataaa tccgttgaga agctgggttg gtactggtta agtcgagtaa
     4621 gaggaaaagt acaatatgca gacctaggag cggaaaactg gaaacctatc agcaacttac
     4681 atgatatgtc atctagtcac tcaaagactt taggctataa gaggctgact aaaagcaatc
     4741 caatctcatg ccaaattcta ttgtataaat ctcgctctaa aggccgaaaa aatcagcgct
     4801 cgacacggac tcattgtcac cacccgtcac ctaaaatcta ctcagcgtcg gcaaaggagc
     4861 catggattct agcaactaac ttacctgttg aaattcgaac acccaaacaa cttgttaata
     4921 tctattcgaa gcgaatgcag attgaagaaa ccttccgaga cttgaaaagt cctgcctacg
     4981 gactaggcct acgccatagc cgaacgagca gctcagagcg ttttgatatc atgctgctaa
     5041 tcgccctgat gcttcaacta acatgttggc ttgcgggcgt tcatgctcag aaacaaggtt
     5101 gggacaagca cttccaggct aacacagtca gaaatcgaaa cgtactctca acagttcgct
     5161 taggcatgga agttttgcgg cattctggct acacaataac aagggaagac ttactcgtgg
     5221 ctgcaaccct actagctcaa aatttattca cacatggtta cgctttgggg aaattatgag
     5281 gggatctctc agcactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     5341 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     5401 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     5461 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     5521 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     5581 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     5641 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     5701 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     5761 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     5821 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     5881 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     5941 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     6001 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     6061 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagggcct gatgcggtat
     6121 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc
     6181 tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca
     6241 tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     6301 cggcatccgc ttacagacaa gctgtgaccg tttccgggag ctgcatgtgt cagaggtttt
     6361 caccgtcatc accgaaacgc gcgaggcagg gtgccttgat gtgggcgccg gcggtcgagt
     6421 ggcgacggcg cggcttgtcc gcgccctggt agattgcctg gccgtaggcc agccattttt
     6481 gagcggccag cggccgcgat aggccgacgc gaagcggcgg ggcgtaggga gcgcagcgac
     6541 cgaagggtag gcgctttttg cagctcttgg gctgtgcgct ggccagacag ttatgcacag
     6601 gccaggcggg ttttaagagt tttaataagt tttaaagagt tttaggcgga aaaatcgcct
     6661 tttttctctt ttatatcagt cacttacatg tgtgaccggt tcccaatgta cggctttggg
     6721 ttcccaatgt acgggttccg gttcccaatg tacggctttg ggttcccaat gtacgtgcta
     6781 tccacaggaa agaggccttt tcgacctttt tcccctgcta gggcaatttg ccctagcatc
     6841 tgctccgtac attaggaacc ggcggatgct tcgccctcga tcaggttgcg gtagcgcatg
     6901 actaggatcg ggccagcctg ccccgcctcc tccttcaaat cgtactccgg caggtcattt
     6961 gacccgatca gcttgcgcac ggtgaaacag aacttcttga actctccggc gctgccactg
     7021 cgttcgtaga tcgtcttgaa caaccatctg gcttctgcct tgcctgcggc gcggcgtgcc
     7081 aggcggtaga gaaaacggcc gatgccggga tcgatcaaaa agtaatcggg gtgaaccgtc
     7141 agcacgtccg ggttcttgcc ttctgtgatc tcgcggtaca tccaatcagc tagctcgatc
     7201 tcgatgtact ccggccgccc ggtttcgctc tttacgatct tgtagcggct aatcaaggct
     7261 tcaccctcgg ataccgtcac caggcggccg ttcttggcct tcttcgtacg ctgcatggca
     7321 acgtgcgtgg tgtttaaccg aatgcaggtt tctaccaggt cgtctttctg ctttccgcca
     7381 tcggctcgcc ggcagaactt gagtacgtcc gcaacgtgtg gacggaacac gcggccgggc
     7441 ttgtctccct tcccttcccg gtatcggttc atggattcgg ttagatggga aaccgccatc
     7501 agtaccaggt cgtaatccca cacactggcc atgccggccg gccctgcgga aacctctacg
     7561 tgcccgtctg gaagctcgta gcggatcacc tcgccagctc gtcggtcacg cttcgacaga
     7621 cggaaaacgg ccacgtccat gatgctgcga ctatcgcggg tgcccacgtc atagagcatc
     7681 ggaacgaaaa aatctggttg ctcgtcgccc ttgggcggct tcctaatcga cggcgcaccg
     7741 gctgccggcg gttgccggga ttctttgcgg attcgatcag cggccgcttg ccacgattca
     7801 ccggggcgtg cttctgcctc gatgcgttgc cgctgggcgg cctgcgcggc cttcaacttc
     7861 tccaccaggt catcacccag cgccgcgccg atttgtaccg ggccggatgg tttgcgaccg
     7921 ctcacgccga ttcctcgggc ttgggggttc cagtgccatt gcagggccgg caggcaaccc
     7981 agccgcttac gcctggccaa ccgcccgttc ctccacacat ggggcattcc acggcgtcgg
     8041 tgcctggttg ttcttgattt tccatgccgc ctcctttagc cgctaaaatt catctactca
     8101 tttattcatt tgctcattta ctctggtagc tgcgcgatgt attcagatag cagctcggta
     8161 atggtcttgc cttggcgtac cgcgtacatc ttcagcttgg tgtgatcctc cgccggcaac
     8221 tgaaagttga cccgcttcat ggctggcgtg tctgccaggc tggccaacgt tgcagccttg
     8281 ctgctgcgtg cgctcggacg gccggcactt agcgtgtttg tgcttttgct cattttctct
     8341 ttacctcatt aactcaaatg agttttgatt taatttcagc ggccagcgcc tggacctcgc
     8401 gggcagcgtc gccctcgggt tctgattcaa gaacggttgt gccggcggcg gcagtgcctg
     8461 ggtagctcac gcgctgcgtg atacgggact caagaatggg cagctcgtac ccggccagcg
     8521 cctcggcaac ctcaccgccg atgcgcgtgc ctttgatcgc ccgcgacacg acaaaggccg
     8581 cttgtagcct tccatccgtg acctcaatgc gctgcttaac cagctccacc aggtcggcgg
     8641 tggcccatat gtcgtaaggg cttggctgca ccggaatcag cacgaagtcg gctgccttga
     8701 tcgcggacac agccaagtcc gccgcctggg gcgctccgtc gatcactacg aagtcgcgcc
     8761 ggccgatggc cttcacgtcg cggtcaatcg tcgggcggtc gatgccgaca acggttagcg
     8821 gttgatcttc ccgcacggcc gcccaatcgc gggcactgcc ctggggatcg gaatcgacta
     8881 acagaacatc ggccccggcg agttgcaggg cgcgggctag atgggttgcg atggtcgtct
     8941 tgcctgaccc gcctttctgg ttaagtacag cgataacctt catgcgttcc ccttgcgtat
     9001 ttgtttattt actcatcgca tcatatacgc agcgaccgca tgacgcaagc tgttttactc
     9061 aaatacacat caccttttta gacggcggcg ctcggtttct tcagcggcca agctggccgg
     9121 ccaggccgcc agcttggcat cagacaaacc ggccaggatt tcatgcagcc gcacggttga
     9181 gccgtgcgcg ggcggctcga acacgtaccc ggccgcgatc atctccgcct cgatctcttc
     9241 ggtaatgaaa aacggttcgt cctggccgtc ctggtgcggt ttcatgcttg ttcctcttgg
     9301 cgttcattct cggcggccgc cagggcgtcg gcctcggtca atgcgtcctc acggaaggca
     9361 ccgcgccgcc tggcctcggt gggcgtcact tcctcgctgc gctcaagtgc gcggtacagg
     9421 gtcgagcgat gcacgccaag cagtgcagcc gcctctttca cggtgcggcc ttcctggtcg
     9481 atcagctcgc gggcgtgcgc gatctgtgcc ggggtgaggg tagggcgggg gccaaacttc
     9541 acgcctcggg ccttggcggc ctcgcgcccg ctccgggtgc ggtcgatgat tagggaacgc
     9601 tcgaactcgg caatgccggc gaacacggtc aacaccatgc ggccggccgg cgtggtggtg
     9661 tcggcccacg gctctgccag gctacgcagg cccgcgccgg cctcctggat gcgctcggca
     9721 atgtccagta ggtcgcgggt gctgcgggcc aggcggtcta gcctggtcac tgtcacaacg
     9781 tcgccagggc gtaggtggtc aagcatcctg gccagctccg ggcggtcgcg cctggtgccg
     9841 gtgatcttct cggaaaacag cttggtgcag ccggccgcgt gcagttcggc ccgttggttg
     9901 gtcaagtcct ggtcgtcggt gctgacgcgg gcatagccca gcaggccagc ggcggcgctc
     9961 ttgttcatgg cgtaatgtct ccggttctag tcgcaagtat tctactttat gcgactaaaa
    10021 cacgcgacaa gaaaacgcca ggaaaagggc agggcggcag cctgtcgcgt aacttaggac
    10081 ttgtgcgaca tgtcgttttc agaagacggc tgcactgaac gtcagaagcc gactgcacta
    10141 tagcagcgga ggggttggat caaagtactt tgatcccgag gggaaccctg tggttggcat
    10201 gcacatacaa atggacgaac ggataaacct tttcacgccc ttttaaatat ccgattattc
    10261 taataaacgc tcttttctct taggtttacc cgccaatata tcctgtcaaa cactgatagt
    10321 ttaaactgaa ggcgggaaac gacaatctga tccaagctca agctgctcta gcattcgcca
    10381 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
    10441 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
    10501 tcacgacgtt gtaaaacgac ggccagtgcc acactgagct cgatatcggc gcgccccggg
    10561 agctcagcgg caaaacacac ctagactaga tttgttttgc taacccaatt gatattaatt
    10621 atatatgatt aatatttata tgtatatgga attggttaat aaaatgcatc tggttcatca
    10681 aagaattata aagacacgtg acattcattt aggataagaa atatggatga tctctttctc
    10741 ttattcagat aattagtaat tacacataac acacaacttt gatgcccaca ttatagtgat
    10801 tagcatgtca ctatgtgtgc atccttttat ttcatacatt aattaacttg gccaatccag
    10861 aagatggaca agtctaggct cgagtacctg caccagccgg gaatcgaacc cgggtctgta
    10921 ccgtggcagg gtactattct accactagac cactggtgct ttgttgcacc gactcggtgc
    10981 cactttgctg tttccagcaa agttgataac ggactagcct tatttaaact tgctatgctg
    11041 tttccagcat agctcttaaa ctgagaccac cggtggaaag cgggcagtga gcgcaacgca
    11101 attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct
    11161 cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat
    11221 gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga ggacctcgag
    11281 ggggggcccg gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
    11341 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
    11401 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
    11461 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgccc gtctccaggg
    11521 gggcacccgt cagtgcattg cgcgatgcat gcactgcctc cattgcggtc acgccgccac
    11581 gttttgcaat cttcacaagt tggcctgtgt ccaactgtaa ggtctcctgc accagccggg
    11641 aatcgaaccc gggtctgtac cgtggcaggg tactattcta ccactagacc actggtgctt
    11701 tgttcctgca ggctgcacac acaattaccg aagcatggtc agcaactatg aacatgaaat
    11761 tataacaata ccaagcaagt acagcagaaa tcagtcaatc aaattattcc aatatgttag
    11821 atacacgatc taaaccacaa taacatcagt gtacctatca acaccaacag tgacctaaca
    11881 aaatagagag tcgcaccgtg ctcgagttca caaaaataat ctgcaggtct atgaactact
    11941 agaattccat cggcaaactg attcaataac tactagcact atcccgagag cacataaggc
    12001 aggttaatta acataggcta gaatttacag gtgcaacagt tgataagata ggcataagca
    12061 agtaaaatat cctgaaagct atgctatgta atcactaatc aacaagacgc agcccttaga
    12121 ttaaacatga acagagtaga atctatgcaa ctagagggac tgaatcaaaa acaacctaag
    12181 caaaatatac ctgaagctgc tacttggatc aacgagaaaa acagagacta aatcctactg
    12241 attgaacaga tcggaacaaa tcacaaccct agcacagttc tacttctaga tccgaaccaa
    12301 taaatacttg gaccacgaac agcatcgctc ataatcacag atccagcata ccacgcgaac
    12361 cccatctaac cctgttcaaa tctaccacag atcgcaaaac ggagactaac aacaacatct
    12421 cacaatcacg aacaggccgg gaaatcaaat caaacgcgag gaacggagag gatcagaaaa
    12481 tcgcatacct tgagaaggcg attcgacgct tcgcttcgag gggagatttt tttttctctg
    12541 cgattttggg gattcgattg atggaggaag aggcgaggag ggggtggggt atttataggt
    12601 ggggaggggg ggactcgtga gtgggcccag gggtgcggtg ggtggggccc agtgagaata
    12661 tagacaaata aagagaggga gaggcggttt ggggagtccg tccccaccac gcaaacgcga
    12721 gaaggaaaga aaggaacgga aagggggcgg gtccagacgg cgccgcggag atatcgagga
    12781 ggtggaaagc tgatgccgtt tgctcacggc ggggttggtg gccgttaaca cgcagcgaag
    12841 cggcgttact gccgtgtacg ttttgtgacc tttcgaaaga gagccccagc ccaagaaagg
    12901 ctggccaggt ttttttgtac tgtgtacgat tacgacggaa gaaaacatac tcgttgtttt
    12961 gtattggtag atactgggcc gtagtaaaat tagaatcagt tgttaattgg ttattatata
    13021 taccatctta agctaccaaa attaataaag ttgtgatggc tatggtatat tttggtagtg
    13081 aattatattg atttggtata tattagaatc aatccaacca caacacaacc aaaaatacct
    13141 gaagctcaat attgatggtg cttttcgact atctagcaaa tgtggtgaat ggggctttat
    13201 aattgaagca gcccaggatg cactttgtgc agagactgat gtgctccggc gcgccgtccg
    13261 agctcagcgc ttatctttaa tcatattcca tagtccatac catagcacat acagtagtta
    13321 tatgctgcag aagagatcca acaaaacatt cacaatggat tatagaaaca tttgtttatt
    13381 cattataatg tgatcttaca ttcatttaat attagaaaaa gccacaaatt cataacacaa
    13441 caagccaaga aaaaaacaca aacttaagca cacaagcttt ttatttgaca caccaaatat
    13501 ttcatcttca tcttcatata ctcgagaagc tcacactttt cttttctttt tgggctcaaa
    13561 ctctgaacca tcagcagtac gctttgaacc tcccgacagc atcttgattt tattctcgcc
    13621 gtttgaatct tgtattacta acgcccatgg cttgtattct ggagcatcgg aggtcaataa
    13681 catcacattc tcgtcggtag actcgtcata agcagtatgc actagaatgt ccgattcggg
    13741 cttgttgccg atcacctcct ccacttcttc agggagcatc aggatgctct cttggatgac
    13801 caactgcttc ccagtctctt tctcaataat atcactcagg ttcgtcgaac cgcctgaccc
    13861 ccccgacccg ccggatagca ttttgatttt gttctcaccg ttagagtcct ggatgaccaa
    13921 cgcccacggc ttgtactcag gtgcatcaga agtcaaaagc atgacattct catccgtgct
    13981 ttcgtcgtac gcggtatgaa caagaatgtc agattcaggc ttgttgccga tgacctcctc
    14041 cacctcctcc ggaagcatga gaatgctctc ttgtataacc agctgtttcc cagtttcctt
    14101 ctcgattata tctgagagat tagtgcttcc tccggagccg ccagagccac cagatcctgc
    14161 cttcttcttc ttcgcctgcc ccgccttctt cgtcgccgct ggccgcttgt cgcccccgag
    14221 ctgagacagg tcgatgcgcg tctcgtagag gccggtaatc gactggtgga tgagggtcgc
    14281 gtccaggacc tccttagtgc ttgtgtacct cttgcgatcg atagttgtgt cgaagtactt
    14341 gaaagcagca ggggcgccga ggttcgtcag ggtgaagaga tgaatgatat tctcagcctg
    14401 ctccctgatt ggcttgtcgc ggtgcttgtt gtacgcggag aggaccttat ccagattcgc
    14461 gtcggccagg atcacgcgct tggagaactc ggaaatctgc tcaatgatct cgtcgaggta
    14521 atgcttgtgc tgctcgacga acagctgctt ctgctcgttg tcctcggggc tgcccttgag
    14581 cttctcgtag tgggaggcca ggtagaggaa gttcacatac ttggacggca gagccagctc
    14641 gttccccttc tgcagctcgc cagcggaagc cagcatccgc ttcctgccgt tctccagctc
    14701 gaagagtgag tacttgggga gcttaatgat caggtccttc ttcacctcct tgtagccctt
    14761 cgcctccagg aaatcgatcg ggttcttctc gaagctggag cgctccataa tcgtgatccc
    14821 cagcagctcc ttcacgctct tgagcttctt ggacttgccc ttctcaacct tcgccacaac
    14881 caggaccgag taggccacag tggggctgtc gaacccgccg tacttcttcg gatcccagtc
    14941 cttcttgcgg gcgatgagct tgtcgctgtt ccgcttaggc agaattgact ccttagagaa
    15001 cccgccagtc tggacctctg tcttcttgac gatattcact tgtggcatgg agagaacctt
    15061 cctgacggtc gcgaaatccc tgcccttgtc ccacacgatc tcccccgtct cgccgttcgt
    15121 ctcgatgagg gggcgcttcc ggatctcgcc attggccaga gtgatctctg tcttgaagaa
    15181 attcataatg ttagagtaga agaagtactt ggcggtagcc ttgccaatct cctgctccga
    15241 cttggcgatc atcttcctca catcgtaaac cttgtagtcc ccgtacacga actcgctctc
    15301 gagctttggg tacttcttga tcagagctgt gccgaccacc gcgttcaggt acgcgtcatg
    15361 ggcatggtgg taattgttga tctcccgaac cttgtagaac tggaaatcct tcctgaagtc
    15421 ggagacgagc tttgacttca gggtgatgac cttcacctcg cggatcagct tgtcattctc
    15481 atcgtactta gtgttcatcc gtgagtcgag aatctgcgca acgtgcttag tgatctgccg
    15541 tgtctcgacc agctgcctct tgatgaagcc cgccttgtcc agctcagaga gcccgcccct
    15601 ctcagccttt gtgaggttat cgaacttccg ctgcgtgatc agcttggcat tcaggagctg
    15661 gcgccagtag ttcttcatct tcttaacgac ctcctctgaa ggaacattat cagacttgcc
    15721 ccggttcttg tccgacctgg tgaggacctt gttgtcaatg gagtcatcct tcaggaatga
    15781 ctgtggaacg atatgatcga cgtcgtaatc gctgagcctg ttaatatcca gctcctggtc
    15841 cacatacata tcgcggccat tctggaggta gtacaggtag agcttctcat tctgcagctg
    15901 cgtgttctcc accgggtgct ccttgaggat ctgggacccc agctccttaa tgccctcctc
    15961 gatcctcttc atcctctcgc gtgagttctt ctggcccttc tgcgtggtct gattctcccg
    16021 ggccatctca atgacgatgt tctcaggctt gtgcctgccc atgaccttca ccagctcgtc
    16081 cacaaccttc acggtctgca gaatcccctt cttgatagct ggcgagccag cgaggttcgc
    16141 gatatgctcg tgcagcgagt ccccctggcc gctcacctga gccttctgga tatcctcctt
    16201 gaatgtgagg ctgtcatcgt gaatcagctg catgaaattg cggttcgcga agccatcgct
    16261 cttcaggaag tcgaggatcg tcttcccgga ctgcttgtcc cgaatgccgt tgatgagctt
    16321 cctgctcagc ctcccccagc cggtgtacct cctcctcttg agctgcttca tgaccttgtc
    16381 atcgaagaga tgggcgtaag tcttcaggcg ctcctcgatc atctcccggt cctcgaacag
    16441 agtgagtgtc agcacaatgt cctcgaggat atcctcattc tcctcgttgt ccaggaagtc
    16501 cttatcctta atgatcttca ggagatcgtg gtaggtcccc agggaggcgt tgaagcggtc
    16561 ctcaacgcca gagatctcga ccgaatcgaa gcactcaatc ttcttgaagt agtcctcctt
    16621 gagctgctta accgtgacct tccggttggt cttgaacagg aggtccacga tggccttctt
    16681 ctgctcccca gacaggaaag ccggcttcct catgccctcg gtcacatact tcaccttagt
    16741 cagctcgttg tagactgtga agtactcgta caggagcgag tgcttaggga gcaccttctc
    16801 atttggcagg ttcttgtcga aattcgtcat cctctcgatg aacgactgag cgctagcgcc
    16861 cttgtcgacc acctcctcga agttccacgg cgtgatcgtc tcctctgact tgcgggtcat
    16921 ccaagcgaag cgggagttgc ccctagcgag tgggccgacg tagtacggga tcctgaaagt
    16981 cagaatcttc tcgatcttct cgcggttatc cttgaggaaa gggtagaagt cctcctgcct
    17041 cctcaggata gcgtgcagct ccccgagatg aatctggtgt gggatgctgc cgttatcgaa
    17101 tgtccgctgc ttcctcagga ggtcctcgcg attgagcttc accagcagct cctccgtgcc
    17161 gtccatcttc tccagaatcg gcttgatgaa cttgtagaac tcctcctgag aggccccgcc
    17221 gtcaatgtac ccagcgtagc cgttcttcga ctgatcgaag aagatctcct tgtacttctc
    17281 ggggagctgc tgcctgacca gcgccttcag gagggtcaga tcctgatggt gctcgtcgta
    17341 gcgcttgatc atggaggctg agagcggagc cttcgtaatc tcggtgttca ccctgagaat
    17401 atcagacagg aggatggcgt ccgacagatt cttggcagcg aggaacaggt ccgcgtactg
    17461 atcgccgatc tgggccagga ggttatccag gtcatcgtcg tatgtgtcct tggagagctg
    17521 cagcttggcg tcctcagcga gatcgaaatt cgacttgaag ttgggcgtga gccccaggct
    17581 gagcgcaatg agattcccga acaggccgtt cttcttctcg cccggcagct gggcgatcag
    17641 gttctcgagg cgccgagact tcgagagcct agcggacagg atagccttcg cgtcgacgcc
    17701 tgacgcatta atggggttct cctcgaagag ctggttgtac gtctgcacga gctggatgaa
    17761 cagcttgtca acatcgctat tgtccgggtt gagatccccc tcgatcagga aatggcccct
    17821 gaacttaatc atgtgggcca gagcgaggta gatcaggcgg aggtccgcct tatctgtgga
    17881 gtccacgagc ttcttccgca gatggtagat cgtagggtac ttctcgtggt aggcaacctc
    17941 gtcgacaatg ttgccgaaga ttggatgccg ctcgtgcttc ttatcctcct ccacgaggaa
    18001 tgactcctcc agcctgtgga agaaagaatc gtcaaccttc gccatctcgt tggagaaaat
    18061 ctcctgcagg tagcagatgc gattcttcct gcgcgtgtac cgcctgcggg cggtgcgctt
    18121 gagccgcgta gcctcagccg tctcgccgct gtcgaacagg agagcgccaa tgagattctt
    18181 cttgatggaa tgccgatcgg tgttgcccag gaccttgaac ttctttgagg gcaccttgta
    18241 ctcgtcggtg atcacggccc agccaacaga gttagtccca atagcgaggc cgatcgagta
    18301 cttcttgtca gcagctggca ccccgtggat gccaaccttc ctcttcttct tcggagccat
    18361 cgaacccccg ctgctgcccc cgctgctttc aggtgttgcg ctctcgcttg tgccaggtgt
    18421 ctcagagcca gaggagcctc cgctagatcc tccggagttg atggagctct gggccttctt
    18481 ctgagcattg aacacctgtc taggcatccg atagaaatcg cacagcaggg cggcacattc
    18541 atctgccagg attccctcgg taatttcgac gcggtgattc atgccggggt agttcagcac
    18601 gttcatcagg gagcctgcgg cgcctctttt tgagttcctc acgccaaaca ccacgcggcc
    18661 gatcctagag ttgatcatgg cgccggcgca catcacgcaa ggctcgaatg tcacgtacag
    18721 ggtggcgtca atcagtcgtg agttctgcat gaccaggccg ccctgtctca gggccataat
    18781 ttcggcatgg gctgttgggt cgtgcaggcc gatgcggcgg ttccagccct cgccgatcac
    18841 tctattgttc agcaccagca cggctcccac aggcgcctcc ccctcatccc gtgccctctt
    18901 ggccagggtc agggcatgtc tcatccagta ctcgtgggaa aactccacct cagagacttt
    18961 ccgcttcttc tttggtgact cgaactcgct tccgtcggct gtccgtttca ttcctgcagg
    19021 ctgcagaagt aacaccaaac aacagggtga gcatcgacaa aagaaacagt accaagcaaa
    19081 taaatagcgt atgaaggcag ggctaaaaaa atccacatat agctgctgca tatgccatca
    19141 tccaagtata tcaagatcaa aataattata aaacatactt gtttattata atagataggt
    19201 actcaaggtt agagcatatg aatagatgct gcatatgcca tcatgtatat gcatcagtaa
    19261 aacccacatc aacatgtata cctatcctag atcgatattt ccatccatct taaactcgta
    19321 actatgaaga tgtatgacac acacatacag ttccaaaatt aataaataca ccaggtagtt
    19381 tgaaacagta ttctactccg atcttgaacg aatgaacgac cgcccaacca caccacatca
    19441 tcacaaccaa gcgaacaaaa agcatctctg tatatgcatc agtaaaaccc gcatcaacat
    19501 gtatacctat cctagatcga tatttccatc catcatcttc aattcgtaac tatgaatatg
    19561 tatggcacac acatacagat ccaaaattaa taaatccacc aggtagtttg aaacagaatt
    19621 aattctactc cgatcttgaa cgaccgccca accagaccac atcatcacaa ccaagacaaa
    19681 aaaaagcatg aaaagatgac ccgacaaaca agtgcacggc atatattgaa ataaaggaaa
    19741 agggcaaacc aaaccctatg caacgaaaca aaaaaaatca tgaaatcgat cccgtctgcg
    19801 gaacggctag agccatccca ggattcccca aagagaaaca ctggcaagtt agcaatcaga
    19861 acgtgtctga cgtacaggtc gcatccgtgt acgaacgcta gtagcacgga tctaacacaa
    19921 acacggatct aacacaaaca tgaacagaag tagaactacc gggccctaac catggaccgg
    19981 aacgccgatc ttgagaaggt agagaggggg gggggggagg acgagcggcg taccttgaag
    20041 cggaggtgcc gacgggtgga tttgggggag ttctggttgt gtgtgtgtgc gctccgaaca
    20101 acacgaggtt ggggaaagag ggtgtggagg gggtgtctat ttattacggc gggcgaggaa
    20161 gggaaagcga aggagcggtg ggaaaggaat cccccgtagc tgccggtgcc gtgagaggag
    20221 gaggaggccg cctgccgtgc cggctcacgt ctgccgctcc gccacgcaat ttctggatgc
    20281 cgacagcgga gcaagtccaa cggtggagcg gaactctcga gaggggtcca gaggcagcga
    20341 cagagatgcc gtgccgtctg cttcgcttgg cccgacgcga cgctgctggt tcgctggttg
    20401 gtgtccgtta gactcgtcga cggcgtttaa caggctggca ttatctactc gaaacaagaa
    20461 aaatgtttcc ttagtttttt taatttctta aagggtattt gtttaatttt tagtcacttt
    20521 attttattct attttatatc taaattatta aataaaaaaa ctaaaataga gttttagttt
    20581 tcttaattta gaggctaaaa tagaataaaa tagatgtact aaaaaaatta gtctataaaa
    20641 accattaacc ctaaacccta aatggatgta ctaataaaat ggatgaagta ttatataggt
    20701 gaagctattt gcaaaaaaaa aggagaacac atgcacacta aaaagataaa actgtagagt
    20761 cctgttgtca aaatactcaa ttgtccttta gaccatgtct aactgttcat ttatatgatt
    20821 ctctaaaaca ctgatattat tgtagtacta tagattatat tattcgtaga gtaaagttta
    20881 aatatatgta taaagataga taaactgcac ttcaaacaag tgtgacaaaa aaaatatgtg
    20941 gtaatttttt ataacttaga catgcaatgc tcaccttatc tcaagagagg ggcacgaccg
    21001 ggtcacgctg cactgcagct ccggcgcgcc gatccccggg taccgagctc gaattcgtaa
    21061 tcatgtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
    21121 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
    21181 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
    21241 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg ctagagcagc ttgccaacat
    21301 ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct cagaagacca
    21361 aagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg gattccattg
    21421 cccagctatc tgtcacttca tcaaaaggac agtagaaaag gaaggtggca cctacaaatg
    21481 ccatcattgc gata
//
