LOCUS       sequence-471436-        2170 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  psaA2 promoter Chlamydomonas reinhardtii.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_2_003_psaA2_prom_Cr
FEATURES             Location/Qualifiers
     source          1..2170
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     204..221
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(245..302)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    424..445
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      448..491
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(495..513)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             675..1334
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(741..760)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1355..1449
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      join(1632..2170,1..50)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2121..2140
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctgtggaaa aacgccagca
       61 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
      121 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
      181 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcctgc ataacgcgaa
      241 gtaatctttt cggttttaaa gaaaaagggc agggtggtga caccttgccc ttttttgccg
      301 gactgcagcg gccgctacta gtcgcgatga gtggtctcaa gtagttatat tataccataa
      361 atatattttt tattggttta caaattattt ttacttattt taaactcccg agaccactca
      421 tcgctctaga agcggccgcg aattccagaa atcatcctta gcgaaagcta aggatttttt
      481 ttatctgaaa ttctgcctcg tgatacgcct atttttatag gttaatgtca tgataataat
      541 ggtttcttag acgtcaggtg gcactcgagt tgatcgggca cgtaagaggt tccaactttc
      601 accataatga aataagatca ctaccgggcg tattttttga gttatcgaga ttttcaggag
      661 ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat
      721 ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga
      781 ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt
      841 atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa tttcgtatgg
      901 caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc
      961 atgagcaaac tgaaacgttt tcatcactct ggagtgaata ccacgacgat ttccggcagt
     1021 ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta
     1081 aagggtttat tgagaatatg tttttcgtat cagccaatcc ctgggtgagt ttcaccagtt
     1141 ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc atgggcaaat
     1201 attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgttt
     1261 gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc gacgagtggc
     1321 agggcggggc gtaatttgat atcgagctcg cttggactcc tgttgataga tccagtaatg
     1381 acctcagaac tccatctgga tttgttcaga acgctcggtt gccgccgggc gttttttatt
     1441 ggtgagaatc caagcctcga gctgtcagac caagtttact catatatact ttagattgat
     1501 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
     1561 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     1621 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     1681 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     1741 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
     1801 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     1861 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     1921 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     1981 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     2041 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     2101 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     2161 cacctctgac
//
