LOCUS       sequence-471474-        2214 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MYC N-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2214)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2214)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_3b_028_MYC-tag
FEATURES             Location/Qualifiers
     source          1..2214
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     182..199
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(223..280)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     CDS             complement(324..353)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(360..389)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(396..425)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    446..467
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      470..513
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(517..535)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             697..1356
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(763..782)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1377..1471
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     primer_bind     2143..2162
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctcgtcaggg gggcggagcc tgtggaaaaa cgccagcaac gcggcctttt tacggttcct
       61 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
      121 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
      181 cagcgagtca gtgagcgagg aagcctgcat aacgcgaagt aatcttttcg gttttaaaga
      241 aaaagggcag ggtggtgaca ccttgccctt ttttgccgga ctgcagcggc cgctactagt
      301 cgcgatgagt ggtctcacat tcccagatct tcctccgaaa tcagtttttg ttcaccattt
      361 aagtcttcct cagaaattaa tttttgctca ccgtttaaat cttcctctga aataagtttt
      421 tgttccatgg cgagaccact catcgctcta gaagcggccg cgaattccag aaatcatcct
      481 tagcgaaagc taaggatttt ttttatctga aattctgcct cgtgatacgc ctatttttat
      541 aggttaatgt catgataata atggtttctt agacgtcagg tggcactcga gttgatcggg
      601 cacgtaagag gttccaactt tcaccataat gaaataagat cactaccggg cgtatttttt
      661 gagttatcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat
      721 accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt
      781 gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta
      841 aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat
      901 gctcatccgg aatttcgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt
      961 cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcact ctggagtgaa
     1021 taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt
     1081 gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt atcagccaat
     1141 ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc
     1201 cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg
     1261 attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta
     1321 caacagtact gcgacgagtg gcagggcggg gcgtaatttg atatcgagct cgcttggact
     1381 cctgttgata gatccagtaa tgacctcaga actccatctg gatttgttca gaacgctcgg
     1441 ttgccgccgg gcgtttttta ttggtgagaa tccaagcctc gagctgtcag accaagttta
     1501 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     1561 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     1621 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     1681 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     1741 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     1801 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     1861 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     1921 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     1981 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     2041 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     2101 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     2161 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatg
//
