LOCUS       sequence-471513-        3029 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  3' Homology rbcL in the M2 position used for genome integration at 
            the rbcL locus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 3029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_6_009_rbcL_3'Hom_Cr
FEATURES             Location/Qualifiers
     source          1..3029
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      351..445
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      628..1216
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1117..1136
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1370..1387
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(1411..1468)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    2449..2470
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     terminator      2473..2516
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(2520..2538)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             join(2700..3029,1..330)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2766..2785)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac
       61 ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtatcagc caatccctgg
      121 gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt
      181 ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag
      241 gttcatcatg ccgtttgtga tggcttccat gtcggcagaa tgcttaatga attacaacag
      301 tactgcgacg agtggcaggg cggggcgtaa tttgatatcg agctcgcttg gactcctgtt
      361 gatagatcca gtaatgacct cagaactcca tctggatttg ttcagaacgc tcggttgccg
      421 ccgggcgttt tttattggtg agaatccaag cctcgagctg tcagaccaag tttactcata
      481 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
      541 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
      601 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
      661 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
      721 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
      781 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
      841 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
      901 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
      961 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     1021 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     1081 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     1141 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     1201 gcggagcctg tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     1261 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     1321 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     1381 gagcgaggaa gcctgcataa cgcgaagtaa tcttttcggt tttaaagaaa aagggcaggg
     1441 tggtgacacc ttgccctttt ttgccggact gcagcggccg ctactagtcg cgatgagtgg
     1501 tctcagtctc gtctctgtct caacgaacac ggctaccttc agtaattttt aaaccatcac
     1561 ctaataatac cgcacctacg ttgtttgctt ctaagttaag tgcaatacct aaagtaccat
     1621 cttcgaattc aagtaattca cctgacattg ctttttctaa accataaata cgagcaatac
     1681 cgtcacctac ttggaaaacg ataccgaaat ctaccatttt cacttctgga gtgtattgtt
     1741 caattaaatc tttaataaga ttactaagtt cttctggagt acgcattgcc ataaaaaaga
     1801 aaaaataaat aaaagattaa aaaagtttat ttttaaaatc tttctcgaga attttaaata
     1861 agtttaaaat tcaacaaaaa tagtgagtgg taagatcact tgttaacaaa agtaatggtt
     1921 cacccttgtc atatttaaat actaaaattc atttgcccga agaggacaaa tttatttatt
     1981 gcattaaaat ccctaagttt acttgcccgt aaggggaagg gggggacgtc cacaggcgtc
     2041 gtaagcaact aaagtttatg acgccgattg ctttgttagg aaaatataaa tatcccataa
     2101 gaaaaggtcc tttaaaggtt ttatggacta aataaaaaag atagcataag cattaaaatc
     2161 atgcaaatta aaaaaaaggt aaatgtattt ataaaaaggt aaatgtattt atatagtatt
     2221 tatattatag cataataata aatatattta taaattgatt gttcttagag ctaaaagaga
     2281 agaacaatgg gtttataggt attttgagat ccagttataa aaatgacttt tgacgtttat
     2341 ggtatataaa cactgcctct aataaagtct actatattgg agaggagtga acagtggcct
     2401 cgcttatccc ctgacaggaa tatacatgga gcgcgagacc actcatcgct ctagaagcgg
     2461 ccgcgaattc cagaaatcat ccttagcgaa agctaaggat tttttttatc tgaaattctg
     2521 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     2581 aggtggcact cgagttgatc gggcacgtaa gaggttccaa ctttcaccat aatgaaataa
     2641 gatcactacc gggcgtattt tttgagttat cgagattttc aggagctaag gaagctaaaa
     2701 tggagaaaaa aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac
     2761 attttgaggc atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata
     2821 ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc
     2881 acattcttgc ccgcctgatg aatgctcatc cggaatttcg tatggcaatg aaagacggtg
     2941 agctggtgat atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa
     3001 cgttttcatc actctggagt gaataccac
//
