LOCUS       sequence-471575-       14766 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning vector for PaqCI Golden Gate assembly of ISYmu1 guides into 
            ISYmu1 plant expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14766)
  AUTHORS   Weiss T, Kamalu M, Shi H, Li Z, Amerasekera J, Zhong Z, Adler BA, 
            Song MM, Vohra K, Wirnowski G, Chitkara S, Ambrose C, Steinmetz N, 
            Sridharan A, Sahagun D, Banfield JF, Doudna JA, Jacobsen SE
  TITLE     Viral delivery of an RNA-guided genome editor for transgene-free 
            germline editing in Arabidopsis.
  JOURNAL   Nat Plants. 2025 Apr 22. doi: 10.1038/s41477-025-01989-9.
  PUBMED    40263581
REFERENCE   2  (bases 1 to 14766)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Plants.
            2025 Apr 22. doi: 10.1038/s41477-025-01989-9."
COMMENT     Sequence Label: pMK025
FEATURES             Location/Qualifiers
     source          1..14766
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             468..1097
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             1526..2599
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      2665..2859
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(3095..3117)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    3203..3343
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     3258..3277
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(3358..3375)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3529..4117)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3609..3628)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4204..4998)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    5423..5447
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    5525..5699
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(5740..6765)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     promoter        complement(6832..7508)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(6875..6895)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    7699..7720
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7735..7740,7741..7758,7759..7765)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7773..7789
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7778..7800
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7797..7813
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7797..7813
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     ncRNA           complement(8464..8531)
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     protein_bind    8543..8667
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(8708..9013)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPASVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(8810..8829)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             complement(9333..10013)
                     /codon_start=1
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQAGRNLE
                     DPAY"
     promoter        complement(join(10067..10073,10074..10091,10092..10097))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(10122..10246)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(11569..11589)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(11596..11643)
                     /codon_start=1
                     /product="two tandem FLAG(R) epitope tags"
                     /label=2xFLAG
                     /translation="DYKDDDDKDYKDDDDK"
     primer_bind     complement(13690..13707)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(13690..13706)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(13699..13721)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    13909..13933
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 ggctgcactg cttggcgtgc atcgctcgac cctgtaccgc gcacttgagc gcagcgagga
       61 agtgacgccc accgaggcca ggcggcgcgg tgccttccgt gaggacgcat tgaccgaggc
      121 cgacgccctg gcggccgccg agaatgaacg ccaagaggaa caagcatgaa accgcaccag
      181 gacggccagg acgaaccgtt tttcattacc gaagagatcg aggcggagat gatcgcggcc
      241 gggtacgtgt tcgagccgcc cgcgcacgtc tcaaccgtgc ggctgcatga aatcctggcc
      301 ggtttgtctg atgccaagct ggcggcctgg ccggccagct tggccgctga agaaaccgag
      361 cgccgccgtc taaaaaggtg atgtgtattt gagtaaaaca gcttgcgtca tgcggtcgct
      421 gcgtatatga tgcgatgagt aaataaacaa atacgcaagg ggaacgcatg aaggttatcg
      481 ctgtacttaa ccagaaaggc gggtcaggca agacgaccat cgcaacccat ctagcccgcg
      541 ccctgcaact cgccggggcc gatgttctgt tagtcgattc cgatccccag ggcagtgccc
      601 gcgattgggc ggccgtgcgg gaagatcaac cgctaaccgt tgtcggcatc gaccgcccga
      661 cgattgaccg cgacgtgaag gccatcggcc ggcgcgactt cgtagtgatc gacggagcgc
      721 cccaggcggc ggacttggct gtgtccgcga tcaaggcagc cgacttcgtg ctgattccgg
      781 tgcagccaag cccttacgac atatgggcca ccgccgacct ggtggagctg gttaagcagc
      841 gcattgaggt cacggatgga aggctacaag cggcctttgt cgtgtcgcgg gcgatcaaag
      901 gcacgcgcat cggcggtgag gttgccgagg cgctggccgg gtacgagctg cccattcttg
      961 agtcccgtat cacgcagcgc gtgagctacc caggcactgc cgccgccggc acaaccgttc
     1021 ttgaatcaga acccgagggc gacgctgccc gcgaggtcca ggcgctggcc gctgaaatta
     1081 aatcaaaact catttgagtt aatgaggtaa agagaaaatg agcaaaagca caaacacgct
     1141 aagtgccggc cgtccgagcg cacgcagcag caaggctgca acgttggcca gcctggcaga
     1201 cacgccagcc atgaagcggg tcaactttca gttgccggcg gaggatcaca ccaagctgaa
     1261 gatgtacgcg gtacgccaag gcaagaccat taccgagctg ctatctgaat acatcgcgca
     1321 gctaccagag taaatgagca aatgaataaa tgagtagatg aattttagcg gctaaaggag
     1381 gcggcatgga aaatcaagaa caaccaggca ccgacgccgt ggaatgcccc atgtgtggag
     1441 gaacgggcgg ttggccaggc gtaagcggct gggttgcctg ccggccctgc aatggcactg
     1501 gaacccccaa gcccgaggaa tcggcgtgag cggtcgcaaa ccatccggcc cggtacaaat
     1561 cggcgcggcg ctgggtgatg acctggtgga gaagttgaag gccgcgcagg ccgcccagcg
     1621 gcaacgcatc gaggcagaag cacgccccgg tgaatcgtgg caagcggccg ctgatcgaat
     1681 ccgcaaagaa tcccggcaac cgccggcagc cggtgcgccg tcgattagga agccgcccaa
     1741 gggcgacgag caaccagatt ttttcgttcc gatgctctat gacgtgggca cccgcgatag
     1801 tcgcagcatc atggacgtgg ccgttttccg tctgtcgaag cgtgaccgac gagctggcga
     1861 ggtgatccgc tacgagcttc cagacgggca cgtagaggtt tccgcagggc cggccggcat
     1921 ggccagtgtg tgggattacg acctggtact gatggcggtt tcccatctaa ccgaatccat
     1981 gaaccgatac cgggaaggga agggagacaa gcccggccgc gtgttccgtc cacacgttgc
     2041 ggacgtactc aagttctgcc ggcgagccga tggcggaaag cagaaagacg acctggtaga
     2101 aacctgcatt cggttaaaca ccacgcacgt tgccatgcag cgtacgaaga aggccaagaa
     2161 cggccgcctg gtgacggtat ccgagggtga agccttgatt agccgctaca agatcgtaaa
     2221 gagcgaaacc gggcggccgg agtacatcga gatcgagcta gctgattgga tgtaccgcga
     2281 gatcacagaa ggcaagaacc cggacgtgct gacggttcac cccgattact ttttgatcga
     2341 tcccggcatc ggccgttttc tctaccgcct ggcacgccgc gccgcaggca aggcagaagc
     2401 cagatggttg ttcaagacga tctacgaacg cagtggcagc gccggagagt tcaagaagtt
     2461 ctgtttcacc gtgcgcaagc tgatcgggtc aaatgacctg ccggagtacg atttgaagga
     2521 ggaggcgggg caggctggcc cgatcctagt catgcgctac cgcaacctga tcgagggcga
     2581 agcatccgcc ggttcctaat gtacggagca gatgctaggg caaattgccc tagcagggga
     2641 aaaaggtcga aaaggtctct ttcctgtgga tagcacgtac attgggaacc caaagccgta
     2701 cattgggaac cggaacccgt acattgggaa cccaaagccg tacattggga accggtcaca
     2761 catgtaagtg actgatataa aagagaaaaa aggcgatttt tccgcctaaa actctttaaa
     2821 acttattaaa actcttaaaa cccgcctggc ctgtgcataa ctgtctggcc agcgcacagc
     2881 cgaagagctg caaaaagcgc ctacccttcg gtcgctgcgc tccctacgcc ccgccgcttc
     2941 gcgtcggcct atcgcggccg ctggccgctc aaaaatggct ggcctacggc caggcaatct
     3001 accagggcgc ggacaagccg cgccgtcgcc actcgaccgc cggcgcccac atcaaggcac
     3061 cctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     3121 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     3181 cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt
     3241 atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
     3301 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt ccgcttcctc
     3361 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     3421 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     3481 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     3541 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     3601 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     3661 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     3721 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     3781 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     3841 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     3901 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     3961 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     4021 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     4081 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     4141 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgcattctag
     4201 gtactaaaac aattcatcca gtaaaatata atattttatt ttctcccaat caggcttgat
     4261 ccccagtaag tcaaaaaata gctcgacata ctgttcttcc ccgatatcct ccctgatcga
     4321 ccggacgcag aaggcaatgt cataccactt gtccgccctg ccgcttctcc caagatcaat
     4381 aaagccactt actttgccat ctttcacaaa gatgttgctg tctcccaggt cgccgtggga
     4441 aaagacaagt tcctcttcgg gcttttccgt ctttaaaaaa tcatacagct cgcgcggatc
     4501 tttaaatgga gtgtcttctt cccagttttc gcaatccaca tcggccagat cgttattcag
     4561 taagtaatcc aattcggcta agcggctgtc taagctattc gtatagggac aatccgatat
     4621 gtcgatggag tgaaagagcc tgatgcactc cgcatacagc tcgataatct tttcagggct
     4681 ttgttcatct tcatactctt ccgagcaaag gacgccatcg gcctcactca tgagcagatt
     4741 gctccagcca tcatgccgtt caaagtgcag gacctttgga acaggcagct ttccttccag
     4801 ccatagcatc atgtcctttt cccgttccac atcataggtg gtccctttat accggctgtc
     4861 cgtcattttt aaatataggt tttcattttc tcccaccagc ttatatacct tagcaggaga
     4921 cattccttcc gtatctttta cgcagcggta tttttcgatc agttttttca attccggtga
     4981 tattctcatt ttagccattt attatttcct tcctcttttc tacagtattt aaagataccc
     5041 caagaagcta attataacaa gacgaactcc aattcactgt tccttgcatt ctaaaacctt
     5101 aaataccaga aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa catagtatcg
     5161 acggagccga ttttgaaacc gcggtgatca caggcagcaa cgctctgtca tcgttacaat
     5221 caacatgcta ccctccgcga gatcatccgt gtttcaaacc cggcagctta gttgccgttc
     5281 ttccgaatag catcggtaac atgagcaaag tctgccgcct tacaacggct ctcccgctga
     5341 cgccgtcccg gactgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt
     5401 cggggagctg ttggctggct ggtggcagga tatattgtgg tgtaaacaaa ttgacgctta
     5461 gacaacttaa taacacattg cggacgtttt taatgtactg aattaacgcc gaattaattc
     5521 gggggatctg gattttagta ctggattttg gttttaggaa ttagaaattt tattgataga
     5581 agtattttac aaatacaaat acatactaag ggtttcttat atgctcaaca catgagcgaa
     5641 accctatagg aaccctaatt cccttatctg ggaactactc acacattatt atggagaaac
     5701 tcgagcttgt cgatcgacag atcccggtcg gcatctactc tatttctttg ccctcggacg
     5761 agtgctgggg cgtcggtttc cactatcggc gagtacttct acacagccat cggtccagac
     5821 ggccgcgctt ctgcgggcga tttgtgtacg cccgacagtc ccggctccgg atcggacgat
     5881 tgcgtcgcat cgaccctgcg cccaagctgc atcatcgaaa ttgccgtcaa ccaagctctg
     5941 atagagttgg tcaagaccaa tgcggagcat atacgcccgg agtcgtggcg atcctgcaag
     6001 ctccggatgc ctccgctcga agtagcgcgt ctgctgctcc atacaagcca accacggcct
     6061 ccagaagaag atgttggcga cctcgtattg ggaatccccg aacatcgcct cgctccagtc
     6121 aatgaccgct gttatgcggc cattgtccgt caggacattg ttggagccga aatccgcgtg
     6181 cacgaggtgc cggacttcgg ggcagtcctc ggcccaaagc atcagctcat cgagagcctg
     6241 cgcgacggac gcactgacgg tgtcgtccat cacagtttgc cagtgataca catggggatc
     6301 agcaatcgcg catatgaaat cacgccatgt agtgtattga ccgattcctt gcggtccgaa
     6361 tgggccgaac ccgctcgtct ggctaagatc ggccgcagcg atcgcatcca tagcctccgc
     6421 gaccggttgt agaacagcgg gcagttcggt ttcaggcagg tcttgcaacg tgacaccctg
     6481 tgaacggcgg gagatgcaat aggtcaggct ctcgctaaac tccccaatgt caagcacttc
     6541 cggaatcggg agcgcggccg atgcaaagtg ccgataaaca taacgatctt tgtagaaacc
     6601 atcggcgcag ctatttaccc gcaggacata tccacgccct cctacatcga agctgaaagc
     6661 acgagattct tcgccctccg agagctgcat caggtcggag acgctgtcga acttttcgat
     6721 cagaaacttc tcgacagacg tcgcggtgag ttcaggcttt ttcatatctc attgcccccc
     6781 ggatctgcga aagctcgaga gagatagatt tgtagagaga gactggtgat ttcagcgtgt
     6841 cctctccaaa tgaaatgaac ttccttatat agaggaaggg tcttgcgaag gatagtggga
     6901 ttgtgcgtca tcccttacgt cagtggagat atcacatcaa tccacttgct ttgaagacgt
     6961 ggttggaacg tcttcttttt ccacgatgct cctcgtgggt gggggtccat ctttgggacc
     7021 actgtcggca gaggcatctt gaacgatagc ctttccttta tcgcaatgat ggcatttgta
     7081 ggtgccacct tccttttcta ctgtcctttt gatgaagtga cagatagctg ggcaatggaa
     7141 tccgaggagg tttcccgata ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc
     7201 tgagactgta tctttgatat tcttggagta gacgagagtg tcgtgctcca ccatgttcac
     7261 atcaatccac ttgctttgaa gacgtggttg gaacgtcttc tttttccacg atgctcctcg
     7321 tgggtggggg tccatctttg ggaccactgt cggcagaggc atcttgaacg atagcctttc
     7381 ctttatcgca atgatggcat ttgtaggtgc caccttcctt ttctactgtc cttttgatga
     7441 agtgacagat agctgggcaa tggaatccga ggaggtttcc cgatattacc ctttgttgaa
     7501 aagtctcaat agccctttgg tcttctgaga ctgtatcttt gatattcttg gagtagacga
     7561 gagtgtcgtg ctccaccatg ttggcaagct gctctagcca atacgcaaac cgcctctccc
     7621 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
     7681 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
     7741 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
     7801 aaacagctat gaccatgatt acgaattcgt tgtcaatcaa ttggcaagtc ataaaatgca
     7861 ttaaaaaata ttttcatact caactacaaa tccatgagta taactataat tataaagcaa
     7921 tgattagaat ctgacaagga ttctggaaaa ttacataaag gaaagttcat aaatgtctaa
     7981 aacacaagag gacatacttg tattcagtaa catttgcagc ttttctaggt ctgaaaatat
     8041 atttgttgcc tagtgaataa gcataatggt acaactacaa gtgttttact cctcatatta
     8101 acttcggtca ttagaggcca cgatttgaca catttttact caaaacaaaa tgtttgcata
     8161 tctcttataa tttcaaattc aacacacaac aaataagaga aaaaacaaat aatattaatt
     8221 tgagaatgaa caaaaggacc atatcattca ttaactcttc tccatccatt tccatttcac
     8281 agttcgatag cgaaaaccga ataaaaaaca cagtaaatta caagcacaac aaatggtaca
     8341 agaaaaacag ttttcccaat gccataatac tcaaactcag taggattctg gtgtgtgcgc
     8401 aatgaaactg atgcattgaa cttgacgaac gttgtcgaaa ccgatgatac gaacgaaagc
     8461 tctgtcccat tcgccatgcc gaagcatgtt gcccagccgg cgccagcgag gaggctggga
     8521 ccatgccggc ccatcgcagg tgaccacttt gtacaagaaa gctgaacgag aaacgtaaaa
     8581 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
     8641 taaaacacaa catatccagt cactatggtc gacctgcaga ctggctgtgt ataagggagc
     8701 ctgacattta tattccccag aacatcaggt taatggcgtt tttgatgtca ttttcgcggt
     8761 ggctgagatc agccacttct tccccgataa cggaggccgg cacactggcc atatcggtgg
     8821 tcatcatgcg ccagctttca tccccgatat gcaccaccgg gtaaagttca cgggggactt
     8881 tatctgacag cagacgtgca ctggccaggg ggatcaccat ccgtcgcccg ggcgtgtcaa
     8941 taatatcact ctgtacatcc acaaacagac gataacggct ctctctttta taggtgtaaa
     9001 ccttaaactg catttcacca gcccctgttc tcgtcagcaa aagagccgtt catttcaata
     9061 aaccgggcga cctcagccat cccttcctga ttttccgctt tccagcgttc ggcacgcaga
     9121 cgacgggctt cattctgcat ggttgtgctt accagaccgg agatattgac atcatatatg
     9181 ccttgagcaa ctgatagctg tcgctgtcaa ctgtcactgt aatacgctgc ttcatagcat
     9241 acctcttttt gacatacttc gggtatacat atcagtatat attcttatac cgcaaaaatc
     9301 agcgcgcaaa tacgcatact gttatctggc ttttagtaag ccggatcctc tagattacgc
     9361 cccgcctgcc actcatcgca gtactgttgt aattcattaa gcattctgcc gacatggaag
     9421 ccatcacaaa cggcatgatg aacctgaatc gccagcggca tcagcacctt gtcgccttgc
     9481 gtataatatt tgcccatggt gaaaacgggg gcgaagaagt tgtccatatt ggccacgttt
     9541 aaatcaaaac tggtgaaact cacccaggga ttggctgaga cgaaaaacat attctcaata
     9601 aaccctttag ggaaataggc caggttttca ccgtaacacg ccacatcttg cgaatatatg
     9661 tgtagaaact gccggaaatc gtcgtggtat tcactccaga gcgatgaaaa cgtttcagtt
     9721 tgctcatgga aaacggtgta acaagggtga acactatccc atatcaccag ctcaccgtct
     9781 ttcattgcca tacggaattc cggatgagca ttcatcaggc gggcaagaat gtgaataaag
     9841 gccggataaa acttgtgctt atttttcttt acggtcttta aaaaggccgt aatatccagc
     9901 tgaacggtct ggttataggt acattgagcg actgactgaa atgcctcaaa atgttcttta
     9961 cgatgccatt gggatatatc aacggtggta tatccagtga tttttttctc cattttagct
    10021 tccttagctc ctgaaaatct cgacggatcc taactcaaaa tccacacatt atacgagccg
    10081 gaagcataaa gtgtaaagcc tggggttcct aatgcggccg ccatagtgac tggatatgtt
    10141 gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat atattgatat
    10201 ttatatcatt ttacgtttct cgttcagctt ttttgtacaa acttgtcacc tgcttttttg
    10261 aaactctcat gactaaagtc acgagattct tgggaacaga gcatacttac cggcttattt
    10321 ctttgccggc agaggtttct cttgtttacc aagctacccc cgcaattcct gcggttcctg
    10381 tttgcttctg tattgctaag accccggagt cagagctctt atcctgagtc cctcagccag
    10441 tatatttctt gctgcattga cgtcacggtc atggtgggca tgacaaacag gacaggtcca
    10501 ctcccttaca tggagaggct tcttcctgtc cttgtggccg cactctgagc atatctgact
    10561 tgacggaaac catctgctta tcctgatgac ttcttttcca taccaggaag ccttgtactg
    10621 cagtttcgat acaaggctcg tccatgatac atcagagatg ctttttgcca gtttatgatt
    10681 gcgcatcatg cccttaacgt taaggtcctc gatacagatg atatcgtggt ttttgactat
    10741 ctctgtactg agtttgttaa gatattcttt tcgctggttt gcaacctttt cataaagtct
    10801 cgctaccttc cgcctttgct tctgatagtt cctggcttca gaaagggaaa tgcccctttt
    10861 ctccgcagca agtgcacgcc ttgcaagctt tcgctgctcc cgtctaagcc tttcttccat
    10921 ttgcctggta aaatgattgt tgtcatgcct gcttccgtct gacataaccg caaagtcaat
    10981 gattccgagg tcaattccga cagatgagtc agtctttggc aattcacaga tctcctcttc
    11041 gcataggatc gatacgaaga atttcccgct cgatttcctt cgtactgtgg cattcagtat
    11101 acggcctttc atctccctac tgtctgcaaa cttgacaagg cccagcttag gcagcttcag
    11161 acaatttccg gaaactgcga tgttgttgtt cgtatattgt gtcgtgtagc tttgcctcgg
    11221 gctcttcttg ctcttgaact ggggatgctc gttctgtcct ttgaaaaagc gggagaaggc
    11281 gtccgacagg ttacgaagcg aattctggag cgagaactta tccacttcac aaagccatac
    11341 tgtttccggg tccctcttaa gctttgtcag aagtttggag catgcgtaat aagtcagacc
    11401 ttttccggtc tcctcatatt ccttgttcca aagttccagg aaatggttgt atacatacct
    11461 tgaactgcct attgtcttgg caatcagtgt ttcctgcttc ttatctggat agatacggta
    11521 ttcataggct ttatgctgca gcatagcagc tggaactccg tggattccaa cctttctctt
    11581 cttctttgga gccatcttat catcatcatc cttgtaatcc ttatcatcat catccttgta
    11641 atccatctgt taatcagaaa aactcagatt aatcgacaaa ttcgatcgca caaactagaa
    11701 actaacacca gatctagata gaaatcacaa atcgaagagt aattattcga caaaactcaa
    11761 attatttgaa caaatcggat gatatctatg aaaccctaat cgagaattaa gatgatatct
    11821 aacgatcaaa cccagaaaat cgtcttcgat ctaagattaa cagaatctaa accaaagaac
    11881 atatacgaaa ttgggatcga acgaaaacaa aatcgaagat tttgagagaa taaggaacac
    11941 agaaatttac cttgatcacg gtagagagaa ttgagagaaa gtttttaaga ttttgagaaa
    12001 ttgaaatctg aattgtgaag aagaagagct ctttgggtat tgttttatag aagaagaaga
    12061 agaaaagacg aggacgacta ggtcacgaga aagctaaggc ggtgaagcaa tagctaataa
    12121 taaaatgaca cgtgtattga gcgttgttta cacgcaaagt tgtttttggc taattgcctt
    12181 atttttaggt tgaggaaaag tatttgtgct ttgagttgat aaacacgact cgtgtgtgcc
    12241 ggctgcaacc actttgacgc cgtttattac tgactcgtcg acaaccacaa tttctaacgg
    12301 tcgtcataag atccagccgt tgagatttaa cgatcgttac gatttatatt tttttagcat
    12361 tatcgtttta ttttttaaat atacggtgga gctgaaaatt ggcaataatt gaaccgtggg
    12421 tcccactgca ttgaagcgta tttcgtattt tctagaattc ttcgtgcttt atttcttttc
    12481 ctttttgttt ttttttgcca tttatctaat gcaagtgggc ttataaaatc agtgaatttc
    12541 ttggaaaagt aacttcttta tcgtataaca tattgtgaaa ttatccattt cttttaattt
    12601 tttagtgtta ttggatattt ttgtatgatt attgatttgc ataggataat gacttttgta
    12661 tcaagttggt gaacaagtct cgttaaaaaa ggcaagtggt ttggtgactc gatttattct
    12721 tgttatttaa ttcatatatc aatggatctt atttggggcc tggtccatat ttaacactcg
    12781 tgttcagtcc aatgaccaat aatatttttt cattaataac aatgtaacaa gaatgataca
    12841 caaaacattc tttgaataag ttcgctatga agaagggaac ttatccggtc ctagatcatc
    12901 agttcataca aacctccata gagttcaaca tcttaaacaa gaatatcctg atctgaagaa
    12961 tgtggaggct ttagtccctt ggatacttgg gaggctgtgg aagaacagaa acgagctggt
    13021 gcttaaaggg agggaatttg gaaccaatga ggtattagta aggacacaag aagatgcaga
    13081 tgagtggatt agaaggaaag aggctcagaa tgtaaggaaa gcaccaacca caacaccgtc
    13141 gggacagacg agaaacatgg gaagctccac cacaatcttg ggttaagtgc aactttgatg
    13201 gggcatggcc aacagaagga ttaaaatgtg gcttagggtg ggtgcttcgc gatcatacag
    13261 ggaaggtgtt atggttaggt gcaagagctg tggtaaaagt aagaagcgtg ctggaagtag
    13321 aagtggaggc tcttagatgg gctgtgctgt cattatcccg attcaattat aggaagatca
    13381 tttttgaggt ggattctcag caacttgtgt ctttggttac atgaaagtta tgcttgtcaa
    13441 gtctcaatcc aattatccaa gacataaagt atctacttag caagtttgag gattttatgc
    13501 ttgtgcatac aagccgagaa ggaaatggag tggcagatag aatagctaag gaatctcttt
    13561 cttttgagaa ttatgatcta aagttgtatt ctattgtacc aatttgggtt aaaagctctg
    13621 ttgagctaga ctggtacctt cctaggatac tagtgatcta gagtcgacct gcaggcatgc
    13681 aagcttggca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
    13741 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
    13801 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgctaga gcagcttgag
    13861 cttggatcag attgtcgttt cccgccttca gtttaaacta tcagtgtttg acaggatata
    13921 ttggcgggta aacctaagag aaaagagcgt ttattagaat aatcggatat ttaaaagggc
    13981 gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc caaccacagg gttcccctcg
    14041 ggatcaaagt actttgatcc aacccctccg ctgctatagt gcagtcggct tctgacgttc
    14101 agtgcagccg tcttctgaaa acgacatgtc gcacaagtcc taagttacgc gacaggctgc
    14161 cgccctgccc ttttcctggc gttttcttgt cgcgtgtttt agtcgcataa agtagaatac
    14221 ttgcgactag aaccggagac attacgccat gaacaagagc gccgccgctg gcctgctggg
    14281 ctatgcccgc gtcagcaccg acgaccagga cttgaccaac caacgggccg aactgcacgc
    14341 ggccggctgc accaagctgt tttccgagaa gatcaccggc accaggcgcg accgcccgga
    14401 gctggccagg atgcttgacc acctacgccc tggcgacgtt gtgacagtga ccaggctaga
    14461 ccgcctggcc cgcagcaccc gcgacctact ggacattgcc gagcgcatcc aggaggccgg
    14521 cgcgggcctg cgtagcctgg cagagccgtg ggccgacacc accacgccgg ccggccgcat
    14581 ggtgttgacc gtgttcgccg gcattgccga gttcgagcgt tccctaatca tcgaccgcac
    14641 ccggagcggg cgcgaggccg ccaaggcccg aggcgtgaag tttggccccc gccctaccct
    14701 caccccggca cagatcgcgc acgcccgcga gctgatcgac caggaaggcc gcaccgtgaa
    14761 agaggc
//
