LOCUS       sequence-471684-       16351 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To clone guide RNA (gRNA) along with its scaffold in to the Foxtail 
            Mosaic Virus Vector (T-DNA).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16351)
  AUTHORS   Baysal C, Kausch AP, Cody JP, Altpeter F, Voytas DF
  TITLE     Rapid and efficient in planta genome editing in sorghum using 
            foxtail mosaic virus-mediated sgRNA delivery.
  JOURNAL   Plant J. 2025 Jan;121(2):e17196. doi: 10.1111/tpj.17196. Epub 2024 
            Dec 11.
  PUBMED    39661735
REFERENCE   2  (bases 1 to 16351)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J."; 
            date: "2025-01"; volume: "121(2):e17196. doi"; pages: " 
            10.1111/tpj.17196. Epub 2024 Dec 11"
COMMENT     Sequence Label: pEE082 FoMV
FEATURES             Location/Qualifiers
     source          1..16351
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(291..1085)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    1510..1534
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    1612..1786
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(1843..2640)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1954..1973)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2564..2583
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(2703..3379)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     protein_bind    3570..3591
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3606..3611,3612..3629,3630..3636)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    3644..3660
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3649..3671
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3668..3684
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3668..3684
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        4049..4394
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     regulatory      4991..5000
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10017..10322
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     10201..10220
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     terminator      11311..11563
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    11581..11605
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             12906..13535
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             13964..15037
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      15103..15297
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(15533..15555)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    15641..15781
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     15696..15715
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(15796..15813)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(15967..16351,1..204))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(16047..16066)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
       61 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
      121 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
      181 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
      241 cagtggaacg aaaactcacg ttaagggatt ttggtcatgc attctaggta ctaaaacaat
      301 tcatccagta aaatataata ttttattttc tcccaatcag gcttgatccc cagtaagtca
      361 aaaaatagct cgacatactg ttcttccccg atatcctccc tgatcgaccg gacgcagaag
      421 gcaatgtcat accacttgtc cgccctgccg cttctcccaa gatcaataaa gccacttact
      481 ttgccatctt tcacaaagat gttgctgtct cccaggtcgc cgtgggaaaa gacaagttcc
      541 tcttcgggct tttccgtctt taaaaaatca tacagctcgc gcggatcttt aaatggagtg
      601 tcttcttccc agttttcgca atccacatcg gccagatcgt tattcagtaa gtaatccaat
      661 tcggctaagc ggctgtctaa gctattcgta tagggacaat ccgatatgtc gatggagtga
      721 aagagcctga tgcactccgc atacagctcg ataatctttt cagggctttg ttcatcttca
      781 tactcttccg agcaaaggac gccatcggcc tcactcatga gcagattgct ccagccatca
      841 tgccgttcaa agtgcaggac ctttggaaca ggcagctttc cttccagcca tagcatcatg
      901 tccttttccc gttccacatc ataggtggtc cctttatacc ggctgtccgt catttttaaa
      961 tataggtttt cattttctcc caccagctta tataccttag caggagacat tccttccgta
     1021 tcttttacgc agcggtattt ttcgatcagt tttttcaatt ccggtgatat tctcatttta
     1081 gccatttatt atttccttcc tcttttctac agtatttaaa gataccccaa gaagctaatt
     1141 ataacaagac gaactccaat tcactgttcc ttgcattcta aaaccttaaa taccagaaaa
     1201 cagctttttc aaagttgttt tcaaagttgg cgtataacat agtatcgacg gagccgattt
     1261 tgaaaccgcg gtgatcacag gcagcaacgc tctgtcatcg ttacaatcaa catgctaccc
     1321 tccgcgagat catccgtgtt tcaaacccgg cagcttagtt gccgttcttc cgaatagcat
     1381 cggtaacatg agcaaagtct gccgccttac aacggctctc ccgctgacgc cgtcccggac
     1441 tgatgggctg cctgtatcga gtggtgattt tgtgccgagc tgccggtcgg ggagctgttg
     1501 gctggctggt ggcaggatat attgtggtgt aaacaaattg acgcttagac aacttaataa
     1561 cacattgcgg acgtttttaa tgtactgaat taacgccgaa ttaattcggg ggatctggat
     1621 tttagtactg gattttggtt ttaggaatta gaaattttat tgatagaagt attttacaaa
     1681 tacaaataca tactaagggt ttcttatatg ctcaacacat gagcgaaacc ctataggaac
     1741 cctaattccc ttatctggga actactcaca cattattatg gagaaactcg agcttgtcga
     1801 tcgactctag ctagaggatc gatccgaacc ccagagtccc gctcagaaga actcgtcaag
     1861 aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg ataccgtaaa gcacgaggaa
     1921 gcggtcagcc cattcgccgc caagttcttc agcaatatca cgggtagcca acgctatgtc
     1981 ctgatagcgg tccgccacac ccagccggcc acagtcgatg aatccagaaa agcggccatt
     2041 ttccaccatg atattcggca agcaggcatc gccatgtgtc acgacgagat cctcgccgtc
     2101 gggcatgcgc gccttgagcc tggcgaacag ttcggctggc gcgagcccct gatgttcttc
     2161 gtccagatca tcctgatcga caagaccggc ttccatccga gtacgtgctc gctcgatgcg
     2221 atgtttcgct tggtggtcga atgggcaggt agccggatca agcgtatgca gccgccgcat
     2281 tgcatcagcc atgatggata ctttctcggc aggagcaagg tgagatgaca ggagatcctg
     2341 ccccggcact tcgcccaata gcagccagtc ccttcccgct tcagtgacaa cgtcgagcac
     2401 agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc cgcgctgcct cgtcctggag
     2461 ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga accgggcgcc cctgcgctga
     2521 cagccgaaac acggcggcat cagagcagcc gattgtctgt tgtgcccagt catagccgaa
     2581 tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat ccatcttgtt caatccccat
     2641 ggtcgatcga cagatctgcg aaagctcgag agagatagat ttgtagagag agactggtga
     2701 tttcagcgtg tcctctccaa atgaaatgaa cttccttata tagaggaagg gtcttgcgaa
     2761 ggatagtggg attgtgcgtc atcccttacg tcagtggaga tatcacatca atccacttgc
     2821 tttgaagacg tggttggaac gtcttctttt tccacgatgc tcctcgtggg tgggggtcca
     2881 tctttgggac cactgtcggc agaggcatct tgaacgatag cctttccttt atcgcaatga
     2941 tggcatttgt aggtgccacc ttccttttct actgtccttt tgatgaagtg acagatagct
     3001 gggcaatgga atccgaggag gtttcccgat attacccttt gttgaaaagt ctcaatagcc
     3061 ctttggtctt ctgagactgt atctttgata ttcttggagt agacgagagt gtcgtgctcc
     3121 accatgttca catcaatcca cttgctttga agacgtggtt ggaacgtctt ctttttccac
     3181 gatgctcctc gtgggtgggg gtccatcttt gggaccactg tcggcagagg catcttgaac
     3241 gatagccttt cctttatcgc aatgatggca tttgtaggtg ccaccttcct tttctactgt
     3301 ccttttgatg aagtgacaga tagctgggca atggaatccg aggaggtttc ccgatattac
     3361 cctttgttga aaagtctcaa tagccctttg gtcttctgag actgtatctt tgatattctt
     3421 ggagtagacg agagtgtcgt gctccaccat gttggcaagc tgctctagcc aatacgcaaa
     3481 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     3541 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     3601 caggctttac actttatgct tccggctcgt ataatgtgtg gaattgtgag cggataacaa
     3661 tttcacacag gaaacagcta tgacatgatt acgaattcga gctcctccaa aaatatcaaa
     3721 gatacagtct cagaagacca aagggcaatt gagacttttc aacaaagggt aatatccgga
     3781 aacctcctcg gattccattg cccagctatc tgtcacttta ttgtgaagat agtggaaaag
     3841 gaaggtggct cctacaaatg ccatcattgc gataaaggaa aggccatcgt tgaagatgcc
     3901 tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt ggaaaaagaa
     3961 gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atactccaaa aatatcaaag
     4021 atacagtctc agaagaccaa agggcaattg agacttttca acaaagggta atatccggaa
     4081 acctcctcgg attccattgc ccagctatct gtcactttat tgtgaagata gtggaaaagg
     4141 aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggccatcgtt gaagatgcct
     4201 ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag
     4261 acgttccaac cacgtcttca aagcaagtgg attgatgtga tatctccact gacgtaaggg
     4321 atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga agttcatttc
     4381 atttggagag ggaaaactct tccgaaaccg aaactgactg aaactacctc gaccgacctt
     4441 agaacccaag aacccaacgg gtgcggccac tatgtccatc gaggcagttt tcgaccaggt
     4501 taccgaccca tcgctccgcg ctgtgattca ggaggaagcg cacaaacaga tcaaagattt
     4561 atttaaggaa acgacgcgct gcaatcccta ctccataccg caagctgggc gcaaggtttt
     4621 ggaaaagtac gccatcccct acaacccgta ctctctcaaa ctacaccctc acgcagcctc
     4681 aaaagcgttt gaagtgtcgc tctacgaggc tgcgtctaac tacctcccct ccacctcctc
     4741 aactcctgtc acattcatgt tcacaaaacc gggcaagctc agattcttta ggcgccgagg
     4801 tcatgtggac aaattcgtta atgctgacat agttccaaga gacttggcta gatacccacg
     4861 cgacacagtc tacagttatc tgcccgagat caccaccaca cacgctttca ttggcgacac
     4921 cctgcatcac ttcggtgagg actttctcgt cgaggttttc tccaggtcac cgaaactaga
     4981 agtgcttcta gccaccatgg tattaccacc cgaagccttt tacagaatgg agtccctcca
     5041 tccctcggtt tacactctcc tctacaggga cgaccgattc ctatacctgc ctggtggcct
     5101 atctggcggt gagtacgaac atcgctataa ggacctaaac tggctaacat ttggcacggt
     5161 tacgcacggc gggatcacta tcacaggaga acgcattgag actaaggccg cgaatcatct
     5221 tttcctcttc agacgagggc gactagcgac accaaaattc cgctcattcg atatgcccga
     5281 gcctatggtc ctgcttccca aggttttccg ccccgcaaag tacaatgtac agaagccaat
     5341 tccccgggag aaagcaaaca aatggttgat gtacgttaaa tccatcggca atgccaccat
     5401 tcgtgacgta tgggctaagc tgaggcaaac catagccaat gcagacattg gactcttctc
     5461 gcccactgag ctcgtgcatc tcacgaatta cttcctgctc ctgggccggc ttgactcaca
     5521 caactccttc gaccaagtac tggccgacag tgtgctgaaa gcatggttca gaccaatggt
     5581 cgcaaagctt caggagatca agcacaaact catggggcag acccaattca tgcaactctg
     5641 ccaagcgcta gagatgacgg aggtggacct cgtctttgag gtccgggact ccaagacccc
     5701 ccacaaacaa gctgtgccat tggaccgtga aattgaaaac gttttgttgg aaggagtctc
     5761 atcggagcca acttacactg aaaccgaagg cgttgctgat ggtccacttc cccccccaat
     5821 gcaaactgca gccgagccgt ccgcgacctc agacgagcct gagagctcta gctcgcgtga
     5881 aattgagcac caaccggcgc ctgagatcac gcttgacgag gaagaacctc agcgagacga
     5941 tctgccttgg gacgcttgga gaacacaatt aagggcgctt ggcttcgagg cctcagaaag
     6001 gcagtatgac ccggacggtg aactgatctc tcctatcctg agcacccgaa ggttgcctaa
     6061 aactcccata aacacaacac tctgcgccac gctagacaag attgcacgct gcccaacttt
     6121 ctacaagcct gacacagatc gcgcgcagac ttacgctcgc gacgtcatgg cggggaaaac
     6181 cggtgctatt ctcaagcaac aaccctttga gtggaaaacc acgctcaagc gcaagactaa
     6241 agaggaaccg aaggaaattc accttgcggt gttgcatggt gcgggcgggt cgggcaaatc
     6301 ctacgcactg caggaattta tgcggaacaa ccctgacaca ccaattacgg tcatcctgcc
     6361 gactaacgag ctcagggccg actggaagaa aaaattgccc gcccacgaca aagacacatt
     6421 tatgacatac gaaaacgcgc tcttgtgccc tcgtggagac atcttcatta tggacgatta
     6481 cacaaaattg cccaggggct acattgaggc tttcgtgcag aatgcacctg ccctctcact
     6541 tctgatactc accggtgacc ccaaccaagc cgaacacttt gagaccactg aggacaatga
     6601 aattaacagc ctcgcccccg cctcagtggt cttcggcaag ttctctaggt accacataaa
     6661 tgccacacac cgcaacccca gaaacttggc aaacgccctc ggtgtttact ccgagacgcc
     6721 cggggaggtt aaagtgcttt acacgaggaa catcaagacc ggttatcaca atctcgtgcc
     6781 ctcacaaatg aagatgagaa attacgcctc actcgggcag cgagcgtcca cctatgcggg
     6841 ttgtcagggg atcaccgcgc cccgcgttca aatcatccta gactccgaca caccccggtg
     6901 caccaggcaa gtcatgtaca ctgcactctc aagggccacg acggaagtgg tgctctgcaa
     6961 cacgatgccg gatgagaaaa gctttttcca gaaggttgaa gcaacaccgt acctcaaagc
     7021 catcctcaac ctcaacaaag agattaaagt cactgagggt gacttgacag aagaaccgcc
     7081 gagggagccc gctcctccca ccacacacct acctgttgaa aacagaatta ttcttaatga
     7141 ggccctagtc gaaccgctgc ccgacaaaca tgaccgcgag atctactcca actccactgg
     7201 cttttcaaac tgcatacaga ctcaagaccc gtacatccaa gccttccaac atcagcaggc
     7261 caaggacgag acattgttct gggcaaccgt cgagaagagg ctcgcagcat ctacgccgaa
     7321 ggacaactgg acagaattca agaccaagag acctctgggt gacgtgcttt ggctcgcgta
     7381 caagcgggcg atggagctcc cagatgagcc catcaaattt aacccagagc tctggtgggc
     7441 atgtgcagac gaggtgcaaa agacctacct ctccaagccc atacacgcac tcaagaacgg
     7501 aatccttcgg caatcacccg actttgactg gaacaaactg cagattttcc tcaagtcaca
     7561 gtgggttaag aagattgaca aaatcgggaa aattgacgtc aacgctggac agacaattgc
     7621 cgccttttac caaccaaccg ttatgctgtt tggaaccatg gcgagataca tgcgccgcat
     7681 ccgcgacact tatcaacccc gcgaaatact catcaattgc gagaagaacc agaagcacat
     7741 ttcgaagtgg gtcgagagca attggaacca ccgcctacct gcttacacca atgacttcac
     7801 cgcttacgac caaagccaag acggggctat gttacagttt gaggtgctga aagccctgca
     7861 ccatgatatc cctcatgagg ttgtggaagc ctacgtagcc cttaaactca actcaaaaat
     7921 gtttctgggc acactggcga ttatgagatt aactggtgag ggacccacct tcgacgctaa
     7981 cacagagtgc aacattgctt acacacacgc ccggttcgag atcccaaaga atgtggcgca
     8041 aatgtacgcg ggtgacgact gtgcgctcaa ctgcaggccc gttgaacggc agtccttcct
     8101 gcctcttgtg gagaaattca ccctgaaatc aaaacctaaa gtatttgagc aaaaagttgg
     8161 gtcatggcct gagttctgcg gcaatctgat caccccacgg ggctacctca aggatcccat
     8221 gaagctacaa cactgcctgc aactggcaca gaggaagaaa ccatccgaac ctgggtcgct
     8281 taaagacgtt gctgagaact acgctatgga cttactaccc acatacgagc taggggatgc
     8341 actctacgaa atcttcgacg agagacaaat gaacgcgcac tatcagtcgg tcaggacgct
     8401 tatcacatgc gcccacacca aagtcctccg agttgcacag gcacttcagg aagactgcac
     8461 cttctttagc tccatctaac aggttttgag ttaggctaac tccactgacg aattgaataa
     8521 caatggatag tgaaatagtt gaacgactaa caaagcttgg tttcgtcaag acttcacaca
     8581 cgcacatcgc tggcgagccc ctcgtgattc acgccgttgc tggggccggt aaaaccaccc
     8641 tccttcggtc cttacttgaa ttacctggag tggaagtctt cacaggcggg gagcacgatc
     8701 ctccaaattt gtcagggaaa tatatccgct gcgctgcacc ccctgtggcc ggtgcataca
     8761 acattcttga cgagtacccc gcgtacccaa attggcgatc gcaaccctgg aacgtcctaa
     8821 tcgccgacaa cctacaatac aaagaaccca cagctcgcgc ccactacaca tgcaatcgca
     8881 ctcaccgcct ggggcagctc actgtcgacg ctttgcgtag ggttggtttc gacatcacct
     8941 ttgccggcac gcagactgaa gactacggat tccaggaagg ccatctctac actagtcaat
     9001 tctacggaca ggtcatttca cttgacacgc aggcccataa gatcgctgtg cgccacggac
     9061 tcgcacccct gtccgcttta gaaacccggg ggctggaatt tgatgagacc actgtgataa
     9121 cgactaaaac ctcgctggag gaagtgaagg acaggcacat ggtctatgtc gctctcacaa
     9181 ggcacaggcg cacctgccat ctctacaccg ctcactttgc gccctccgcc tgacaacacg
     9241 aaagccatac taactgtagc tataggaata gccgcctccc tagtcttttt catgctcaca
     9301 cgcaacaatc tgccacacgt cggtgataac atccactcac taccccacgg aggaagttat
     9361 attgacggta ccaagtccat caactaccgc ccacctgcgt cacgctaccc ctcatccaac
     9421 ttactcgctt tcgctccacc aatactcgcc gcagtactct ttttcctcac acagccatat
     9481 ctagctacca gacgatccag gtgcgttcgg tgcttcgttg tccacggggc atgcacgaat
     9541 cacacctagt tgttatatta gcgctgttac ttatagctct gtggtgtcta agcactcgac
     9601 ccgttcaacc atcgtgccac gtcgaaatca acggccactc catcatcgtc accggaaact
     9661 gctggcactc cacacagcgt ccccactgag gaagagcaag ccagataaca gtatgcgtat
     9721 ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg aagtatgtca
     9781 aaaggaggta tgctatgaag cagcgtatta cagtgacagt tgacagcgac agctatcagt
     9841 tgctcaaggc atatatgatg tcaatatctc cggtctggta agcacaacca tgcagaatga
     9901 agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat caggaaggga tggctgaggt
     9961 cgcccggttt attgaaatga acggctcttt tgctgacgag aacaggggct ggtgaaatgc
    10021 agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga
    10081 gtgatattat tgacacgccc gggcgacgga tggtgatccc cctggccagt gcacgtctgc
    10141 tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat gaaagctggc
    10201 gcatgatgac caccgatatg gccagtgtgc cggtttccgt tatcggggaa gaagtggctg
    10261 atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat
    10321 aagctcttcg gccccactca acgaccgcat tgagggtgtt agggtaacca acatcagtga
    10381 agagaaacaa cccacctcaa gtgtgacctc atcatttcag gacacaatgg caacacaaaa
    10441 tgccgacgtc actgatgcga cggactacaa gaaaccgcct gctgaaactg agcagaaggc
    10501 actcaccatt caaccacggt caaacaaggc gcccagtgac gaggagttgg tacgcatcat
    10561 caacgcggcg cagaagcgag gcctcacacc cgcggccttt gttcaagcag ctatagtctt
    10621 caccatggaa tccatggaca agggcgccac cgactccacg attttcacgg gaaaatacaa
    10681 cactttccca atgaaaagtc tggcgctagc ttgcaaagat gctggcgtgc ccgtgcacaa
    10741 actttgctac ttctatacca agccggctta cgcgaaccgt agggtcgcca accagccgcc
    10801 tgctcgctgg actaacgaga atgtgcccaa agctaacaag tgggcggctt tcgacacctt
    10861 cgacgcactt ctcgacccat acgtagtccc atcctctgta ccgtacgatg agcccacgcc
    10921 agaggatcgc caagtcaatg agattttcaa gaaggacaat ttgagtcagg cagcatccag
    10981 aaaccaactc ctaggaacgc aagcctccat cacgcgcggg agactcaacg gcgcaccagc
    11041 actaccaaac aacgggcagt acttcatcga ggcacctcag taatctagaa gatgggactc
    11101 gagcgatcag tagtatgata ccaataaata aatcgggcga atccgcgcct cctgactatg
    11161 ggcaggttta cggaccaagc tgtatcgaga tacgacctaa cagtaacgca gctaaggggt
    11221 gaatgcacac atcgcttata aaaaaaaaaa aaaaaaaaaa aaacctggat cctaggttca
    11281 caaagtgtca tcgatagctc gaatttcccc gatcgttcaa acatttggca ataaagtttc
    11341 ttaagattga atcctgttgc cggtcttgcg atgattatca tataatttct gttgaattac
    11401 gttaagcatg taataattaa catgtaatgc atgacgttat ttatgagatg ggtttttatg
    11461 attagagtcc cgcaattata catttaatac gcgatagaaa acaaaatata gcgcgcaaac
    11521 taggataaat tatcgcgcgc ggtgtcatct atgttactag atcgggaaac tatcagtgtt
    11581 tgacaggata tattggcggg taaacctaag agaaaagagc gtttattaga ataatcggat
    11641 atttaaaagg gcgtgaaaag gtttatccgt tcgtccattt gtatgtgcat gccaaccaca
    11701 gggttcccct cgggatcaaa gtactttgat ccaacccctc cgctgctata gtgcagtcgg
    11761 cttctgacgt tcagtgcagc cgtcttctga aaacgacatg tcgcacaagt cctaagttac
    11821 gcgacaggct gccgccctgc ccttttcctg gcgttttctt gtcgcgtgtt ttagtcgcat
    11881 aaagtagaat acttgcgact agaaccggag acattacgcc atgaacaaga gcgccgccgc
    11941 tggcctgctg ggctatgccc gcgtcagcac cgacgaccag gacttgacca accaacgggc
    12001 cgaactgcac gcggccggct gcaccaagct gttttccgag aagatcaccg gcaccaggcg
    12061 cgaccgcccg gagctggcca ggatgcttga ccacctacgc cctggcgacg ttgtgacagt
    12121 gaccaggcta gaccgcctgg cccgcagcac ccgcgaccta ctggacattg ccgagcgcat
    12181 ccaggaggcc ggcgcgggcc tgcgtagcct ggcagagccg tgggccgaca ccaccacgcc
    12241 ggccggccgc atggtgttga ccgtgttcgc cggcattgcc gagttcgagc gttccctaat
    12301 catcgaccgc acccggagcg ggcgcgaggc cgccaaggcc cgaggcgtga agtttggccc
    12361 ccgccctacc ctcaccccgg cacagatcgc gcacgcccgc gagctgatcg accaggaagg
    12421 ccgcaccgtg aaagaggcgg ctgcactgct tggcgtgcat cgctcgaccc tgtaccgcgc
    12481 acttgagcgc agcgaggaag tgacgcccac cgaggccagg cggcgcggtg ccttccgtga
    12541 ggacgcattg accgaggccg acgccctggc ggccgccgag aatgaacgcc aagaggaaca
    12601 agcatgaaac cgcaccagga cggccaggac gaaccgtttt tcattaccga agagatcgag
    12661 gcggagatga tcgcggccgg gtacgtgttc gagccgcccg cgcacggctc aaccgtgcgg
    12721 ctgcatgaaa tcctggccgg tttgtctgat gccaagctgg cggcctggcc ggccagcttg
    12781 gccgctgaag aaaccgagcg ccgccgtcta aaaaggtgat gtgtatttga gtaaaacagc
    12841 ttgcgtcatg cggtcgctgc gtatatgatg cgatgagtaa ataaacaaat acgcaagggg
    12901 aacgcatgaa ggttatcgct gtacttaacc agaaaggcgg gtcaggcaag acgaccatcg
    12961 caacccatct agcccgcgcc ctgcaactcg ccggggccga tgttctgtta gtcgattccg
    13021 atccccaggg cagtgcccgc gattgggcgg ccgtgcggga agatcaaccg ctaaccgttg
    13081 tcggcatcga ccgcccgacg attgaccgcg acgtgaaggc catcggccgg cgcgacttcg
    13141 tagtgatcga cggagcgccc caggcggcgg acttggctgt gtccgcgatc aaggcagccg
    13201 acttcgtgct gattccggtg cagccaagcc cttacgacat atgggccacc gccgacctgg
    13261 tggagctggt taagcagcgc attgaggtca cggatggaag gctacaagcg gcctttgtcg
    13321 tgtcgcgggc gatcaaaggc acgcgcatcg gcggtgaggt tgccgaggcg ctggccgggt
    13381 acgagctgcc cattcttgag tcccgtatca cgcagcgcgt gagctaccca ggcactgccg
    13441 ccgccggcac aaccgttctt gaatcagaac ccgagggcga cgctgcccgc gaggtccagg
    13501 cgctggccgc tgaaattaaa tcaaaactca tttgagttaa tgaggtaaag agaaaatgag
    13561 caaaagcaca aacacgctaa gtgccggccg tccgagcgca cgcagcagca aggctgcaac
    13621 gttggccagc ctggcagaca cgccagccat gaagcgggtc aactttcagt tgccggcgga
    13681 ggatcacacc aagctgaaga tgtacgcggt acgccaaggc aagaccatta ccgagctgct
    13741 atctgaatac atcgcgcagc taccagagta aatgagcaaa tgaataaatg agtagatgaa
    13801 ttttagcggc taaaggaggc ggcatggaaa atcaagaaca accaggcacc gacgccgtgg
    13861 aatgccccat gtgtggagga acgggcggtt ggccaggcgt aagcggctgg gttgcctgcc
    13921 ggccctgcaa tggcactgga acccccaagc ccgaggaatc ggcgtgagcg gtcgcaaacc
    13981 atccggcccg gtacaaatcg gcgcggcgct gggtgatgac ctggtggaga agttgaaggc
    14041 cgcgcaggcc gcccagcggc aacgcatcga ggcagaagca cgccccggtg aatcgtggca
    14101 agcggccgct gatcgaatcc gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc
    14161 gattaggaag ccgcccaagg gcgacgagca accagatttt ttcgttccga tgctctatga
    14221 cgtgggcacc cgcgatagtc gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg
    14281 tgaccgacga gctggcgagg tgatccgcta cgagcttcca gacgggcacg tagaggtttc
    14341 cgcagggccg gccggcatgg ccagtgtgtg ggattacgac ctggtactga tggcggtttc
    14401 ccatctaacc gaatccatga accgataccg ggaagggaag ggagacaagc ccggccgcgt
    14461 gttccgtcca cacgttgcgg acgtactcaa gttctgccgg cgagccgatg gcggaaagca
    14521 gaaagacgac ctggtagaaa cctgcattcg gttaaacacc acgcacgttg ccatgcagcg
    14581 tacgaagaag gccaagaacg gccgcctggt gacggtatcc gagggtgaag ccttgattag
    14641 ccgctacaag atcgtaaaga gcgaaaccgg gcggccggag tacatcgaga tcgagctagc
    14701 tgattggatg taccgcgaga tcacagaagg caagaacccg gacgtgctga cggttcaccc
    14761 cgattacttt ttgatcgatc ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc
    14821 cgcaggcaag gcagaagcca gatggttgtt caagacgatc tacgaacgca gtggcagcgc
    14881 cggagagttc aagaagttct gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc
    14941 ggagtacgat ttgaaggagg aggcggggca ggctggcccg atcctagtca tgcgctaccg
    15001 caacctgatc gagggcgaag catccgccgg ttcctaatgt acggagcaga tgctagggca
    15061 aattgcccta gcaggggaaa aaggtcgaaa aggcctcttt cctgtggata gcacgtacat
    15121 tgggaaccca aagccgtaca ttgggaaccg gaacccgtac attgggaacc caaagccgta
    15181 cattgggaac cggtcacaca tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc
    15241 cgcctaaaac tctttaaaac ttattaaaac tcttaaaacc cgcctggcct gtgcataact
    15301 gtctggccag cgcacagccc aagagctgca aaaagcgcct acccttcggt cgctgcgctc
    15361 cctacgcccc gccgcttcgc gtcggcctat cgcggccgct ggccgctcaa aaatggctgg
    15421 cctacggcca ggcaatctac cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg
    15481 gcgcccacat caaggcaccc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac
    15541 acatgcagct cccggaaacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag
    15601 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac
    15661 gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag
    15721 agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag
    15781 gccctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
    15841 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
    15901 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
    15961 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
    16021 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
    16081 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
    16141 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
    16201 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
    16261 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
    16321 cactggtaac aggattagca gagcgaggta t
//
