LOCUS       sequence-471892-        6057 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express cMyc-LgBiT Fusion Protein in Mammalian Cells under a CMV 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6057)
  AUTHORS   Promega
  TITLE     Promega Plasmid Collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6057)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: NanoBiT Assay Vector, cMyc
FEATURES             Location/Qualifiers
     source          1..6057
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    828..876
                     /note="synthetic polyadenylation signal"
     CDS             complement(940..1734)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1051..1070)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1661..1680
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(1766..1813)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(1828..2185)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1842..1977
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        1994..2185
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     2142..2162
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     polyA_signal    2430..2551
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2467..2486)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2521..2540
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2588..2606
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(3225..4541)
                     /codon_start=1
                     /product="human c-Myc proto-oncogene"
                     /label=c-Myc
                     /translation="MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAP
                     SEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTE
                     LLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPN
                     PARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDS
                     LLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGS
                     PSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNR
                     KCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKA
                     TAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA"
     primer_bind     complement(4554..4573)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4555..4573)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4615..4634)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          4617..4749
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        complement(4885..5088)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4914..4934)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5089..5468
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    5659..5942
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
ORIGIN
        1 ttgaaatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgggta
      721 gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat gtcagctact gggctatctg
      781 gacaagggaa aacgcaagcg caaagaggat ccttatcgct atcgattcac acaaaaaacc
      841 aacacacaga tgtaatgaaa ataaagatat tttattgcgg ccatcgtgat ggcaggttgg
      901 gcgtcgcttg gtcggtcatt tcgaacccca gagtcccgct cagaagaact cgtcaagaag
      961 gcgatagaag gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg
     1021 gtcagcccat tcgccgccaa gctcttcagc aatatcacgg gtagccaacg ctatgtcctg
     1081 atagcggtcc gccacaccca gccggccaca gtcgatgaat ccagaaaagc ggccattttc
     1141 caccatgata ttcggcaagc aggcatcgcc atgggtcacg acgagatcct cgccgtcggg
     1201 catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg agcccctgat gctcttcgtc
     1261 cagatcatcc tgatcgacaa gaccggcttc catccgagta cgtgctcgct cgatgcgatg
     1321 tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc
     1381 atcagccatg atggatactt tctcggcagg agcaaggtga gatgacagga gatcctgccc
     1441 cggcacttcg cccaatagca gccagtccct tcccgcttca gtgacaacgt cgagcacagc
     1501 tgcgcaagga acgcccgtcg tggccagcca cgatagccgc gctgcctcgt cctgcagttc
     1561 attcagggca ccggacaggt cggtcttgac aaaaagaacc gggcgcccct gcgctgacag
     1621 ccggaacacg gcggcatcag agcagccgat tgtctgttgt gcccagtcat agccgaatag
     1681 cctctccacc caagcggccg gagaacctgc gtgcaatcca tcttgttcaa tcatgatctg
     1741 cctcctgggt ttagttcctc accttgtcgt attatactat gccgatatac tatgccgatg
     1801 attaattgtc aacagttaat taagcttttt gcaaaagcct aggcctccaa aaaagcctcc
     1861 tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat aaataaaaaa
     1921 aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg gcgggatggg
     1981 cggagttagg ggcgggacta tggttgctga ctaattgaga tgcatgcttt gcatacttct
     2041 gcctgctggg gagcctgggg actttccaca cctggttgct gactaattga gatgcatgct
     2101 ttgcatactt ctgcctgctg gggagcctgg ggactttcca caccctaact gacacacatt
     2161 ccacagctgg ttctttccgc ctcagaaggt acctaaccaa gttcctcttt cagaggttat
     2221 ttcaggccat ggtgctgcgc agatccgcgt atgcggtgtg aaataccgca cagatgcgta
     2281 aggagaaaat accgcatcag gaaattgtaa gcgttaatat tttgttaaaa ttcgatttta
     2341 ccacatttgt agaggtttta cttgcttaaa aaaacctccc acatctcccc ctgaacctga
     2401 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
     2461 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     2521 gtggtttgtc caaactcatc aatgtatctt atcatgtctg ctcgaagcgg ccgccccaag
     2581 gggttatgct agttattgct cagcggtggc agcagccaac tcagcttcct ttcgggcttt
     2641 gttagcagcc ggatcagctt gcatgcctgc aggtcgactc tagagtcgcg gccttagctg
     2701 ttgatggtta ctcggaacag catggagccg tcgggggtga tcaggcgctc gtcgataatt
     2761 ttgttgccgt tccacagggt ccctgttaca gtgatctttt tgccgtcgaa cacggcgatg
     2821 ccttcatacg gccgtccgaa atagttcagc atgttcggcg taaccccgtc gattaccagt
     2881 gtgccatagg gcaggatcac cttaaagtga tgatcatcca cagggtacac caccttaaac
     2941 acctcttcga tctgggccat ttggtcggcg ctcagacctt catacgggat gatgacatgg
     3001 atgtcgatct tcagggcatt ttcaccgctc cggacaatcc tttggatcgg agttacggac
     3061 acggcgagat tctgcagcaa actggacaca cctccctgtt caaggacttg gtccaggttg
     3121 taggcggctg tctgttccca gtccccaacg aaatcttcga gtgtgaagac accgctcgag
     3181 cctccacctc cgctcccgcc accacctgaa ctgccttgag aaaccgcaca agagttccgt
     3241 agctgttcaa gtttgtgttt caactgttct cgtcgtttcc gcaacaagtc ctcttcagaa
     3301 atgagctttt gctcctctgc ttggacggac aggatgtatg ctgtggcttt tttaaggata
     3361 actaccttgg gggccttttc attgttttcc aactccggga tctggtcacg cagggcaaaa
     3421 aagctccgtt ttagctcgtt cctcctctgg cgctccaaga cgttgtgtgt tcgcctcttg
     3481 acattctcct cggtgtccga ggacctgggg ctggtgcatt ttcggttgtt gctgatctgt
     3541 ctcaggactc tgacactgtc caacttgacc ctcttggcag caggatagtc cttccgagtg
     3601 gagggaggcg ctgcgtagtt gtgctgatgt gtggagacgt ggcacctctt gaggaccagt
     3661 gggctgtgag gaggtttgct gtggcctcca gcagaaggtg atccagactc tgaccttttg
     3721 ccaggagcct gcctcttttc cacagaaaca acatcgattt cttcctcatc ttcttgttcc
     3781 tcctcagagt cgctgctggt ggtgggcggt gtctcctcat ggagcaccag gggctcgggg
     3841 ctgccctgcg gggaggactc cgtcgaggag agcagagaat ccgaggacgg agagaaggcg
     3901 ctggagtctt gcgaggcgca ggacttgggc gagctgctgt cgttgagagg gtaggggaag
     3961 accaccgagg ggtcgatgca ctctgaggcg gcggcgctca gatcctgcag gtacaagctg
     4021 gaggtggagc agacgctgtg gccgcgggcg gggttcgggc tgccgctgtc tttgcgcgca
     4081 gcctggtagg aggccagctt ctctgagacg agcttggcgg cggccgagaa gccgctccac
     4141 atacagtcct ggatgatgat gtttttgatg aaggtctcgt cgtccgggtc gcagatgaaa
     4201 ctctggttca ccatgtctcc tcccagcagc tcggtcacca tctccagctg gtcggccgtg
     4261 gagaagctcc cgccaccgcc gtcgttgtct ccccgaaggg agaagggtgt gaccgcaacg
     4321 taggagggcg agcagagccc ggagcggcgg ctaggggaca ggggcggggt gggcagcagc
     4381 tcgaatttct tccagatatc ctcgctgggc gccgggggct gcagctcgct ctgctgctgc
     4441 tgctggtaga agttctcctc ctcgtcgcag tagaaatacg gctgcaccga gtcgtagtcg
     4501 aggtcatagt tcctgttggt gaagctaacg ttgaggggca tggcgatcgc tagccctata
     4561 gtgagtcgta ttaatactct agccttaaga gctgtaattg aactgggagt ggacacctgt
     4621 ggagagaaag gcaaagtgga tgtcagtaag accaataggt gcctatcaga aacgcaagag
     4681 tcttctctgt ctcgacaagc ccagtttcta ttggtctcct taaacctgtc ttgtaacctt
     4741 gatacttacc tgcccagtgc ctcacgacca acttctgcag cttaagttcg agactgttgt
     4801 gtcagaagca ctgactgcgt tagcaattta actgtgataa actaccgcaa taaagcttct
     4861 agtgatctga cggttcacta aaccagctct gcttatatag acctcccacc gtacacgcct
     4921 accgcccatt tgcgtcaacg gggcggggtt attacgacat tttggaaagt cccgttgatt
     4981 ttggtgccaa aacaaactcc cattgacgtc aatggggtgg agacttggaa atccccgtga
     5041 gtcaaaccgc tatccacgcc cattggtgta ctgccaaaac cgcatcacca tggtaatagc
     5101 gatgactaat acgtagatgt actgccaagt aggaaagtcc cgtaaggtca tgtactgggc
     5161 ataatgccag gcgggccatt taccgtcatt gacgtcaata gggggcggac ttggcatatg
     5221 atacacttga tgtactgcca agtgggcagt ttaccgtaaa tactccaccc attgacgtca
     5281 atggaaagtc cctattggcg ttactatggg aacatacgtc attattgacg tcaatgggcg
     5341 ggggtcgttg ggcggtcagc caggcgggcc atttaccgta agttatgtaa cgcggaactc
     5401 catatatggg ctatgaacta atgaccccgt aattgattac tattaataac tagtcaataa
     5461 tcaatgccaa catggcggtc atattggaca tgagccaata taaatgtaca tattatgata
     5521 tagatacaac gtatgcaatg gccaatagcc aatattgatt tatgctatat aaccaatgaa
     5581 taatatggct aatggccaat attgaagatc tcgatcctct acgccggacg catcgtggcc
     5641 ggagtactgc ggtttatctc gatggctacg agggcagaca gtaagtggat ttaccataat
     5701 cccttaattg tacgcaccgc taaaacgcgt tcagcgcgat cacggcagca gacaggtaaa
     5761 aatggcaaca aaccacccga aaaactgccg caatcgcgcc tgataaattt taaccgtatg
     5821 aatacctatg caaccagagg gtacaggcca cattaccccc acttaatcca ctgaagctgc
     5881 catttttcat ggtttcacca tcccagcgaa gggccatcca gcgtgcgttc ctgtatttcc
     5941 ggactataaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     6001 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttc
//
