LOCUS       sequence-471992-       11338 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  BEVA Golden Gate cloning vector; BEVA2.0 Broad host range plasmid 
            with stability. L1-RSF1010-Sp-par-ELT4; GG assembly from pGQ0015, 
            pNDGG002, pNDGG005, pOGG012, and pOGG014, SpR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11338)
  AUTHORS   Geddes BA, Williamson R, Schumacher J, Ardi A, Levin G, Cervenka E, 
            Huang R, diCenzo G
  TITLE     BEVA2.0: modular assembly of golden gate-compatible vectors with 
            expanded utility for genetic engineering.
  JOURNAL   Can J Microbiol. 2025 Jan 1;71:1-13. doi: 10.1139/cjm-2024-0246.
  PUBMED    39919271
REFERENCE   2  (bases 1 to 11338)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Can J 
            Microbiol."; date: "2025-01-1"; volume: "71:1-13. doi"; pages: " 
            10.1139/cjm-2024-0246"
COMMENT     Sequence Label: pNDGG058
FEATURES             Location/Qualifiers
     source          1..11338
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     oriT            140..248
                     /note="incP origin of transfer"
     rep_origin      935..1329
                     /label=RSF1010 oriV
                     /note="replication origin of the broad-host-range plasmid 
                     RSF1010; requires the RSF1010 RepA/B/C proteins for 
                     replication (Scholz et al., 1989)"
     oriT            1671..1757
                     /label=RSF1010 oriT
                     /note="origin of transfer of the broad-host-range plasmid 
                     RSF1010 (Scholz et al., 1989)"
     CDS             2996..3967
                     /codon_start=1
                     /gene="repB"
                     /product="replication protein B of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepB
                     /translation="MKNDRTLQAIGRQLKAMGCERFDIGVRDATTGQMMNREWSAAEVL
                     QNTPWLKRMNAQGNDVYIRPAEQERHGLVLVDDLSEFDLDDMKAEGREPALVVETSPKN
                     YQAWVKVADAAGGELRGQIARTLASEYDADPASADSRHYGRLASFTNRKDKHTTRAGYQ
                     PWVLLRESKGKTATAGPALVQQAGQQIEQAQRQQEKARRLASLELPERQLSRHRRTALD
                     EYRSEMAGLVKRFGDDLSKCDFIAAQKLASRGRSAEETGKAMAEASPALAERKPGHEAD
                     YIERTVSKVMGLPSVQLARAELARAPAPRQRGMDRGGPDFSM"
     CDS             4478..5317
                     /codon_start=1
                     /gene="repA"
                     /product="replication protein A of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepA
                     /translation="MATHKPINILEAFAAAPPPLDYVLPNMVAGTVGALVSPGGAGKSM
                     LALQLAAQIAGGPDLLEVGELPTGPVIYLPAEDPPTAIHHRLHALGAHLSAEERQAVAD
                     GLLIQPLIGSLPNIMAPEWFDGLKRAAEGRRLMVLDTLRRFHIEEENASGPMAQVIGRM
                     EAIAADTGCSIVFLHHASKGAAMMGAGDQQQASRGSSVLVDNIRWQSYLSSMTSAEAEE
                     WGVDDDQRRFFVRFGVSKANYGAPFADRWFRRHDGGVLKPAVLERQRKSKGVPRGEA"
     CDS             5304..6155
                     /codon_start=1
                     /gene="repC"
                     /product="replication protein C of the broad-host-range 
                     plasmid RSF1010 (Scholz et al., 1989)"
                     /label=RSF1010 RepC
                     /translation="MVKPKNKHSLSHVRHDPAHCLAPGLFRALKRGERKRSKLDVTYDY
                     GDGKRIEFSGPEPLGADDLRILQGLVAMAGPNGLVLGPEPKTEGGRQLRLFLEPKWEAV
                     TADAMVVKGSYRALAKEIGAEVDSGGALKHIQDCIERLWKVSIIAQNGRKRQGFRLLSE
                     YASDEADGRLYVALNPLIAQAVMGGGQHVRISMDEVRALDSETARLLHQRLCGWIDPGK
                     TGKASIDALCGYVWPSEASGSTMRKRRQRVREALPELVALGWTVTEFAAGKYDITRPKA
                     AG"
     terminator      complement(8738..8824)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    8912..8933
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8948..8953,8954..8971,8972..8978)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8986..9002
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8991..9013
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9010..9026
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9010..9026
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             9022..9345
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
                     LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
                     DAA"
     misc_feature    9039..9095
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(9096..9113)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9096..9112)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9105..9127)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(9321..9340)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     terminator      9450..9544
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             10288..11079
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
ORIGIN
        1 actacttttc cgctgcataa ccctgcttcg gggtcattat agcgattttt tcggtatatc
       61 catccttttt cgcacgatat acaggatttt gccaaagggt tcgtgtagac tttccttggt
      121 gtatccaacg gcgtcagccg ggcaggatag gtgaagtagg cccacccgcg agcgggtgtt
      181 ccttcttcac tgtcccttat tcgcacctgg cggtgctcaa cgggaatcct gctctgcgag
      241 gctggccgta ggccggccgc gatcaagcag gtgcgacaga cgtcatacta gatatcaagc
      301 gacttctcct atcccctggg aacacatcaa tctcaccgga gaatatcgct ggccaaagcc
      361 ttagcgtagg attccgcccc ttcccgcaaa cgaccccaaa caggaaacgc agctgaaacg
      421 ggaagctcaa cacccactga cgcatgggtt gttcaggcag tacttcatca accagcaagg
      481 cggcactttc ggccatccgc cgcgccccac agctcgggca gaaaccgcga cgcttacagc
      541 tgaaagcgac caggtgctcg gcgtggcaag actcgcagcg aacccgtaga aagccatgct
      601 ccagccgccc gcattggaga aattcttcaa attcccgttg cacatagccc ggcaattcct
      661 ttccctgctc tgccataagc gcagcgaatg ccgggtaata ctcgtcaacg atctgataga
      721 gaagggtttg ctcgggtcgg tggctctggt aacgaccagt atcccgatcc cggctggccg
      781 tcctggccgc cacatgaggc atgttccgcg tccttgcaat actgtgttta catacagtct
      841 atcgcttagc ggaaagttct tttaccctca gccgaaatgc ctgccgttgc tagacattgc
      901 cagccagtgc ccgtcactcc cgtactaact gtcacgaacc cctgcaataa ctgtcacgcc
      961 cccctgcaat aactgtcacg aacccctgca ataactgtca cgcccccaaa cctgcaaacc
     1021 cagcaggggc gggggctggc ggggtgttgg aaaaatccat ccatgattat ctaagaataa
     1081 tccactaggc gcggttatca gcgcccttgt ggggcgctgc tgcccttgcc caatatgccc
     1141 ggccagaggc cgggtagctg gtctattcgc tgcgctaggc tacacaccgc cccaccgctg
     1201 cgcggcaggg ggaaaggcgg gcaaagcccg ctaaacccca caccaaaccc cgcagaaata
     1261 cgctggagcg cttttagccg ctttagcggc ctttccccct acccgaaggg tgggggcgcg
     1321 tgtgcagccc cgcagggcct gtctcggtcg atcattcagc ccggctcatc cttctggcgt
     1381 ggcggcagac cgaacaaggc gcggtcgtgg tcgcgttcaa ggtacgcatc cattgccgcc
     1441 atgagccgat cctccggcca ctcgctgctg ttcaccttgg ccaaaatcat ggcccccacc
     1501 agcaccctgc gccttgtttc gttcttgcgc tcttgctgct gttcccttgc ccgcacccgc
     1561 tgaatttcgg cattgattcg cgctcgttgt tcttcgagct tggccagccg atccgccgcc
     1621 ttgttgctcc ccttaaccat cttgacaccc cattgttaat gtgctgtctc gtaggctatc
     1681 atggaggcac agcggcggca atcccgaccc tactttgtag gggaggggca cttaccggtt
     1741 tctcttcgag aaactggcct aacggccacc cttcgggcgg tgcgctctcc gagggccatt
     1801 gcatggagcc gaaaagcaaa agcaacagcg aggcagcatg gcgatttatc accttacggc
     1861 gaaaaccggc agcaggtcgg gcggccaatc ggccagggcc aaggccgact acatccagcg
     1921 cgaaggcaag tatgcccgcg acatggatga agtcttgcac gccgaatccg ggcacatgcc
     1981 ggagttcgtc gagcggcccg ccgactactg ggatgctgcc gacctgtatg aacgcgccaa
     2041 tgggcggctg ttcaaggagg tcgaatttgc cctgccggtc gagctgaccc tcgaccagca
     2101 gaaggcgctg gcgtccgagt tcgcccagca cctgaccggt gccgagcgcc tgccgtatac
     2161 gctggccatc catgccggtg gcggcgagaa cccgcactgc cacctgatga tctccgagcg
     2221 gatcaatgac ggcatcgagc ggcccgccgc tcagtggttc aagcggtaca acggcaagac
     2281 cccggagaag ggcggggcac ggaagaccga agcgctcaag cccaaggcat ggcttgagca
     2341 gacccgcgag gcatgggccg accatgccaa ccgggcatta gagcgggctg gccacgacgc
     2401 ccgcattgac cacagaacac ttgaggcgca gggcatcgag cgcctgcccg gtgttcacct
     2461 ggggccgaac gtggtggaga tggaaggccg gggcatccgc accgaccggg cagacgtggc
     2521 cctgaacatc gacaccgcca acacccagat catcgactta caggaatacc gggaggcaat
     2581 agaccatgaa cgcaatcgac agagtgaaga aatccagagg catcaacgag ttagcggagc
     2641 agatcgaacc gctggcccag agcatggcga cactggccga cgaagcccgg caggtcatga
     2701 gccagaccca gcaggccagc gaggcgcagg cggcggagtg gctgaaagcc cagcgccaga
     2761 caggggcggc atgggtggag ctggccaaag agttgcggga ggtagccgcc gaggtgagca
     2821 gcgccgcgca gagcgcccgg agcgcgtcgc gggggtggca ctggaagcta tggctaaccg
     2881 tgatgctggc ttccatgatg cctacggtgg tgctgctgat cgcatcgttg ctcttgctcg
     2941 acctgacgcc actgacaacc gaggacggct cgatctggct gcgcttggtg gcccgatgaa
     3001 gaacgacagg actttgcagg ccataggccg acagctcaag gccatgggct gtgagcgctt
     3061 cgatatcggc gtcagggacg ccaccaccgg ccagatgatg aaccgggaat ggtcagccgc
     3121 cgaagtgctc cagaacacgc catggctcaa gcggatgaat gcccagggca atgacgtgta
     3181 tatcaggccc gccgagcagg agcggcatgg tctggtgctg gtggacgacc tcagcgagtt
     3241 tgacctggat gacatgaaag ccgagggccg ggagcctgcc ctggtagtgg aaaccagccc
     3301 gaagaactat caggcatggg tcaaggtggc cgacgccgca ggcggtgaac ttcgggggca
     3361 gattgcccgg acgctggcca gcgagtacga cgccgacccg gccagcgccg acagccgcca
     3421 ctatggccgc ttggcgagct tcaccaaccg caaggacaag cacaccaccc gcgccggtta
     3481 tcagccgtgg gtgctgctgc gtgaatccaa gggcaagacc gccaccgctg gcccggcgct
     3541 ggtgcagcag gctggccagc agatcgagca ggcccagcgg cagcaggaga aggcccgcag
     3601 gctggccagc ctcgaactgc ccgagcggca gcttagccgc caccggcgca cggcgctgga
     3661 cgagtaccgc agcgagatgg ccgggctggt caagcgcttc ggtgatgacc tcagcaagtg
     3721 cgactttatc gccgcgcaga agctggccag ccggggccgc agtgccgagg aaaccggcaa
     3781 ggccatggcc gaggccagcc cagcgctggc agagcgcaag cccggccacg aagcggatta
     3841 catcgagcgc accgtcagca aggtcatggg tctgcccagc gtccagcttg cgcgggccga
     3901 gctggcacgg gcaccggcac cccgccagcg aggcatggac aggggcgggc cagatttcag
     3961 catgtagtgc ttgcgttggt actcacgcct gttatactat gagtactcac gcacagaagg
     4021 gggttttatg gaatacgaaa aaagcgcttc agggtcggtc tacctgatca aaagtgacaa
     4081 gggctattgg ttgcccggtg gctttggtta tacgtcaaac aaggccgagg ctggccgctt
     4141 ttcagtcgct gatatggcca gccttaacct tgacggctgc accttgtcct tgttccgcga
     4201 agacaagcct ttcggccccg gcaagtttct cggtgactga tatgaaagac caaaaggaca
     4261 agcagaccgg cgacctgctg gccagccctg acgctgtacg ccaagcgcga tatgccgagc
     4321 gcatgaaggc caaagggatg cgtcagcgca agttctggct gaccgacgac gaatacgagg
     4381 cgctgcgcga gtgcctggaa gaactcagag cggcgcaggg cgggggtagt gaccccgcca
     4441 gcgcctaacc accaactgcc tgcaaaggag gcaatcaatg gctacccata agcctatcaa
     4501 tattctggag gcgttcgcag cagcgccgcc accgctggac tacgttttgc ccaacatggt
     4561 ggccggtacg gtcggggcgc tggtgtcgcc cggtggtgcc ggtaaatcca tgctggccct
     4621 gcaactggcc gcacagattg caggcgggcc ggatctgctg gaggtgggcg aactgcccac
     4681 cggcccggtg atctacctgc ccgccgaaga cccgcccacc gccattcatc accgcctgca
     4741 cgcccttggg gcgcacctca gcgccgagga acggcaagcc gtggctgacg gcctgctgat
     4801 ccagccgctg atcggcagcc tgcccaacat catggccccg gagtggttcg acggcctcaa
     4861 gcgcgccgcc gagggccgcc gcctgatggt gctggacacg ctgcgccggt tccacatcga
     4921 ggaagaaaac gccagcggcc ccatggccca ggtcatcggt cgcatggagg ccatcgccgc
     4981 cgataccggg tgctctatcg tgttcctgca ccatgccagc aagggcgcgg ccatgatggg
     5041 cgcaggcgac cagcagcagg ccagccgggg cagctcggta ctggtcgata acatccgctg
     5101 gcagtcctac ctgtcgagca tgaccagcgc cgaggccgag gaatggggtg tggacgacga
     5161 ccagcgccgg ttcttcgtcc gcttcggtgt gagcaaggcc aactatggcg caccgttcgc
     5221 tgatcggtgg ttcaggcggc atgacggcgg ggtgctcaag cccgccgtgc tggagaggca
     5281 gcgcaagagc aagggggtgc cccgtggtga agcctaagaa caagcacagc ctcagccacg
     5341 tccggcacga cccggcgcac tgtctggccc ccggcctgtt ccgtgccctc aagcggggcg
     5401 agcgcaagcg cagcaagctg gacgtgacgt atgactacgg tgacggcaag cggatcgagt
     5461 tcagcggccc ggagccgctg ggcgctgatg atctgcgcat cctgcaaggg ctggtggcca
     5521 tggctgggcc taatggccta gtgcttggcc cggaacccaa gaccgaaggc ggacggcagc
     5581 tccggctgtt cctggaaccc aagtgggagg ccgtcaccgc tgatgccatg gtggtcaaag
     5641 gtagctatcg ggcgctggca aaggaaatcg gggcagaggt cgatagtggt ggggcgctca
     5701 agcacataca ggactgcatc gagcgccttt ggaaggtatc catcatcgcc cagaatggcc
     5761 gcaagcggca ggggtttcgg ctgctgtcgg agtacgccag cgacgaggcg gacgggcgcc
     5821 tgtacgtggc cctgaacccc ttgatcgcgc aggccgtcat gggtggcggc cagcatgtgc
     5881 gcatcagcat ggacgaggtg cgggcgctgg acagcgaaac cgcccgcctg ctgcaccagc
     5941 ggctgtgtgg ctggatcgac cccggcaaaa ccggcaaggc ttccatagat gccttgtgcg
     6001 gctatgtctg gccgtcagag gccagtggtt cgaccatgcg caagcgccgc cagcgggtgc
     6061 gcgaggcgtt gccggagctg gtcgcgctgg gctggacggt aaccgagttc gcggcgggca
     6121 agtacgacat cacccggccc aaggcggcag gctgaccccc cccactctat tgtaaacaag
     6181 acatttttat cttttatatt caatggctta ttttcctgct aattggtaat accatgaaaa
     6241 ataccatgct cagaaaaggc ttaacaatat tttgaaaaat tgcctactga gcgctgccgc
     6301 acagctccat aggccgttac tgcatgagct tgtggaagtg tgctgagcat aaatgattct
     6361 cctagctgtt ctttgggtaa gtacgccatc aggacgttgt gagtggcgcg atttttagcg
     6421 gctgaaatca gcccttgagc ctgtcggcaa gtcgcgtcat gaggtccatg cgctcatgca
     6481 ggatcgccac gaccaacgcg ggttcgcccg cacgcggcag gcaaaaaacg tagtggtgtt
     6541 cgcagcgggc catccgcagc gcgggaaaga gttcgctcat gtccttaaac gggccttcgc
     6601 cggcggcaag cctggctatg ccctgttcca gcttagcgat atagcggcgc acctgcgccg
     6661 cgccccactc ccggcgcgtg tagcggatga tgccgcgtag atcggcttcg gcctcagccg
     6721 tgaggatgta ggccgtcaag cgcgatcccc gctgagttct tcatcaagaa tttcgccgac
     6781 gctcttggtg gacaccttgc cggcaagccc atcgttgatg cggttcccca gcatggtttt
     6841 cagttcctgc catgcctgat cggcatcagc gtcaccgggg aacagacgtt cgagggcgta
     6901 ttgcttaatg gtcttgccct gcaaggcggc cagggctttc aggctctggt gctgctggtc
     6961 cgtcatgtcg attgtcaggc ggctcattgg ataacctcca taaaatacac gtaaccacat
     7021 tagcacatat gtgggcgtga ggctacagcg cgaggcgcat taaggtcggg aaaatgcgct
     7081 aggcgcattt aaattgcgta ttgctgtaat gcgccatgcc ggctagacta ggcccaaatg
     7141 ggtataccca atttgaccaa gggggacgcg atgagggcgg ccaagcacta ccgacaactt
     7201 ctatccatcg acttcaacat cgaggcgctg gccttcgtgc ctggacccga cggcacacgc
     7261 ggccggcgca tccacgtcct ggggcgcgag gtccgcgacc ggcccggcct ggtcgagtac
     7321 ctttcgccgg cgttcggctc gcgggtggcg ctggacggct actgcaaggc caatttcgat
     7381 gcagtgctgc acctggcgta ccccgatcat cagcaatggg gccacgcatg aagcgccgaa
     7441 gctacgccat gctgcgcgcc gctgccgcgc tggccgtcct ggtcgttgcc tcgccggcat
     7501 gggccgagct gcgcggcgag gtcgtgcgca tcatcgacgg cgacaccatc gacgtgctgg
     7561 tagacaagca gccggtgcgc gtgcgcctgg tggacattga cgcgccggaa aagcggcaag
     7621 ccttcggcga acgtgcgcgc caggcgctgg ccggcatggt gttccgccgg cacgtcctgg
     7681 tcgacgagaa ggacaccgac cgttacggcc gcacgctggg caccgtctgg gtcaacatgg
     7741 agctggccag ccggccgccg cagccgcgca acgtcaacgc cgcgatggtt caccagggca
     7801 tggcgtgggc ctatcgcttc cacggccgcg cggccgaccc tgaaatgctg cggctcgaac
     7861 aggaggcgcg aggcaagcgc gtcggcctct ggtccgatcc gcacgccgtc gagccgtgga
     7921 aatggcgacg cgagagcaac aaccggaggg acgaaggttg aaggtcgccc gcatctacct
     7981 gcgcgccagt acggacgagc agaatcttga acgccaggag agccttgtag cggccacgcg
     8041 ggccgccggg tactacgtcg ccggcatcta ccgcgagaag gcgtccggcg cacgcgccga
     8101 ccggcccgag ctgctgcgca tgatcgcgga cctgcaacct ggtgaagtcg tcgttgcgga
     8161 gaagatcgac cgcatcagcc gcttgccgtt ggccgaggcc gagcgcctgg ttgcgtcgat
     8221 ccgggccaaa ggggccaagc tggccgtgcc tggcgtggtg gacctgtcgg agctggccgc
     8281 cgaggcgaac ggagtggcga aaatcgttct ggaatccgtc caggacatgc ttttgaagct
     8341 cgccttgcag atggcccgcg acgactacga ggatcggcgc gagcgtcaac gtcagggtgt
     8401 ccagttggcg aaggccgccg gccgctacac cggccgcaaa cgtgacgccg gcatgcacga
     8461 ccgcatcatc acgcttcgct ccggcggatc gagcattgcc aagacggcca agctggtcgg
     8521 atgcagcccg agccaggtca aacgagtgtg ggcggcctgg aacgcgcagc agcaaaaata
     8581 aagccgggca gtgcccggct tttctcacct tttcgcgtcc cgcagggccg ctgcgagcgc
     8641 cctacctaga tcctcgcttt ccccctcggt gtagtccggc cagggcacga agggcgcgga
     8701 tgcgaacctg ttgagcaggt cagatctagg gcggcggatt tgtcctactc aggagagcgt
     8761 tcaccgacaa acaacagata aaacgaaagg cccagtcttt cgactgagcc tttcgtttta
     8821 tttgatgcct gccgaattcg gatccggagt gagaccgcag ctggcacgac aggtttgccg
     8881 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     8941 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     9001 aatttcacac aggaaacagc tatgaccatg attacgccaa gcttgcatgc ctgcaggtcg
     9061 actctagagg atccccgggt accgagctcg aattcactgg ccgtcgtttt acaacgtcgt
     9121 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     9181 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     9241 aatggcgaat ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     9301 cgcatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     9361 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     9421 agacaagctg tgacggtctc acgctcttgg actcctgttg atagatccag taatgacctc
     9481 agaactccat ctggatttgt tcagaacgct cggttgccgc cgggcgtttt ttattggtga
     9541 gaatccaggc aaccggatca attcccctgc tcgcgcaggc tgggtgccaa gctctcgggt
     9601 aacatcaagg cccgatcctt ggagcccttg ccctcccgca cgatgatcgt gccgtgatcg
     9661 aaatccagat ccttgacccg cagttgcaaa ccctcactga tccgcatgcc cgttccatac
     9721 agaagctggg cgaacaaacg atgctcgcct tccagaaaac cgaggatgcg aaccacttca
     9781 tccggggtca gcaccaccgg caagcgccgc gacggccgag gtcttgcgat ctcctgaagc
     9841 cagggcagat ccgtgcacag caccttgccg tagaagaaca gcaaggccgc caatgcctga
     9901 cgatgcgtgg agagcgaaac cttgcgctcg ttcgccagcc aggacagaaa tgcctcgact
     9961 tcgctgctgc ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc
    10021 cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc
    10081 aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg
    10141 gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg
    10201 cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc
    10261 agtcgcccta aaacaaagtt aaacatcatg agggaagcgg tgatcgccga agtatcgact
    10321 caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta
    10381 catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg
    10441 gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg
    10501 gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt
    10561 gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa
    10621 tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg
    10681 gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag
    10741 gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg
    10801 ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc
    10861 cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg
    10921 gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat
    10981 cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac
    11041 tacgtgaaag gcgagatcac caaggtagtc ggcaaataat gtctaacaat tcgttcaagc
    11101 cgacgccgct tcgcggcgcg gcttaactca agcgttagat gcactaagca cataattgct
    11161 cacagccaaa ctatcaggtc aagtctgctt ttattatttt taagcgtgca taataagccc
    11221 tacacaaatt gggagatata tcatgaaagg ctggcttttt cttgttatcg caatagttgg
    11281 cgaagtaatc gcaacatccg cattaaaatc tagcgagggc tttactaagc tgatccgg
//
