LOCUS       sequence-47201-e         776 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Young CL, Raden DL, Caplan JL, Czymmek KJ, Robinson AS
  TITLE     Cassette series designed for live-cell imaging of proteins and 
            high-resolution techniques in yeast.
  JOURNAL   Yeast. 2012 Mar;29(3-4):119-36. doi: 10.1002/yea.2895. Epub 2012 Apr
            4.
  PUBMED    22473760
REFERENCE   2  (bases 1 to 776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "2012-03"; volume: "29(3-4):119-36. doi"; pages: " 
            10.1002/yea.2895. Epub 2012 Apr 4"
COMMENT     Sequence Label: pCY 3050-07
FEATURES             Location/Qualifiers
     source          1..776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(71..99)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     694..714
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 ggatcccggg cttaagatcg gtgacggtgc tggtttaatt aacagtaaag gagaagaact
       61 tttcactgga gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt
      121 ttctgtcagt ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaattgat
      181 ttgcactact ggaaaactac ctgttccatg gccaacactt gtcactactt tgggttatgg
      241 tctaatgtgc tttgctagat acccagatca tatgaaacgg catgactttt tcaagagtgc
      301 catgcccgaa ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa
      361 gacacgtgct gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg
      421 tattgatttt aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc
      481 acacaatgta tacatcactg cagacaaaca aaagaatgga atcaaagcta acttcaaaat
      541 tagacacaac attgaagatg gaggtgttca actagcagac cattatcaac aaaatactcc
      601 aattggcgat ggccctgtcc ttttaccaga caaccattac ctgtcctatc aatctgccct
      661 ttcgaaagat cccaacgaaa agagagacca catggtcctt cttgagtttg taacagctgc
      721 tgggattaca catggcatgg atgaactata caaattcgaa cacgacgaat tgtagg
//
