LOCUS       sequence-47208-e         806 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Young CL, Raden DL, Caplan JL, Czymmek KJ, Robinson AS
  TITLE     Cassette series designed for live-cell imaging of proteins and 
            high-resolution techniques in yeast.
  JOURNAL   Yeast. 2012 Mar;29(3-4):119-36. doi: 10.1002/yea.2895. Epub 2012 Apr
            4.
  PUBMED    22473760
REFERENCE   2  (bases 1 to 806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "2012-03"; volume: "29(3-4):119-36. doi"; pages: " 
            10.1002/yea.2895. Epub 2012 Apr 4"
COMMENT     Sequence Label: pCY 3080-04
FEATURES             Location/Qualifiers
     source          1..806
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             45..758
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSRLSKDPNEKRDHMVLLEF
                     VTAAGITHGIDELYK"
ORIGIN
        1 tccccgggtt aattaacggt gacggtgctg gtttaattaa cntgtctaaa ggtgaagaat
       61 tattcactgg tgttgtccca attttggttg aattagatgg tgatgttaat ggtcacaaat
      121 tttctgtctc cggtgaaggt gaaggtgatg ctacttacgg taaattgacc ttaaaattta
      181 tttgtactac tggtaaattg ccagttccat ggccaacctt agtcactact ttaggttatg
      241 gtttgatgtg ttttgctaga tacccagatc atatgaaaca acatgacttt ttcaagtctg
      301 ccatgccaga aggttatgtt caagaaagaa ctattttttt caaagatgac ggtaactaca
      361 agaccagagc tgaagtcaag tttgaaggtg ataccttagt taatagaatc gaattaaaag
      421 gtattgattt taaagaagat ggtaacattt taggtcacaa attggaatac aactataact
      481 ctcacaatgt ttacatcatg gctgacaaac aaaagaatgg tatcaaagtt aacttcaaaa
      541 ttagacacaa cattgaagat ggttctgttc aattagctga ccattatcaa caaaatactc
      601 caattggtga tggtccagtc ttgttaccag acaaccatta cttatcctat caatctagat
      661 tatccaaaga tccaaacgaa aagagagacc acatggtctt gttagaattt gttactgctg
      721 ctggtattac ccatggtatt gatgaattgt acaaataagg cgcgccactt ctaaataagc
      781 gaatttctta tgatttatga ttttta
//
