LOCUS       sequence-472129-        3495 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 3495)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC25A11_STOP
FEATURES             Location/Qualifiers
     source          1..3495
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(383..482)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(498..515)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(498..514)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(507..529)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      578..664
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      756..783
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     complement(942..959)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1113..1701)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1193..1212)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(1848..2657)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     2571..2590
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     2649..2668
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     2770..2786
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2770..2786
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2791..2810
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2791..2809
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2827..2926
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ccgatcacgg ccttcagcag aaagccaggt ggggttccgt ctgctcctgt cagtctctcg
       61 aacagcacgg tatagatgcc cagtctggtt gtggtgtatg tggcctgcct cagcagtccg
      121 gcgctcagtc cggtatagat tcccctcagt ccctctgcct tcaggatgga tgtcagggcg
      181 tgaaagctgg tcttgtactc tcttgtcttg gcgccctcgc cagacagctg catccggttc
      241 ttcaccagat ccagaggctg cacgaacacg gttgctccca ttcctgccag tcctccaaac
      301 aggaacttca cggacttggg gctggtcctt ggcttgccgt cgatgcctcc tgctccggcg
      361 gatgctgttg ctgccatggt ggagcctgct tttttgtaca aagttggcat tataaaaaag
      421 cattgctcat caatgtgttg caacgaacag gtcactatca gtcaaaataa aatcattatt
      481 tggggcccga gcttaagact ggccgtcgtt ttacaacgtc gtgactggga aaacatccat
      541 gctagcgtta acgcgagagt agggaactgc caggcatcaa ataaaacgaa aggctcagtc
      601 ggaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac
      661 aaatccgccg ggagcggatt tgaacgttgt gaagcaacgg cccggagggt ggcgggcagg
      721 acgcccgcca taaactgcca ggcatcaaac taagcagaag gccatcctga cggatggcct
      781 ttttgcgttt ctacaaactc ttcctggcta gcggtacgcg tattaattgc gttgcgctca
      841 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
      901 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
      961 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     1021 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     1081 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     1141 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     1201 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     1261 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     1321 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     1381 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     1441 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     1501 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     1561 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     1621 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     1681 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     1741 tggaacgacg cgtaactcac gttaagggat tttggtcatg agcttgcgcc gtcccgtcaa
     1801 gtcagcgtaa tgctctgcca gtgttacaac caattaacca attctgatta gaaaaactca
     1861 tcgagcatca aatgaaactg caatttattc atatcaggat tatcaatacc atatttttga
     1921 aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc agttccatag gatggcaaga
     1981 tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa tacaacctat taatttcccc
     2041 tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag tgacgactga atccggtgag
     2101 aatggcaaaa gtttatgcat ttctttccag acttgttcaa caggccagcc attacgctcg
     2161 tcatcaaaat cactcgcatc aaccaaaccg ttattcattc gtgattgcgc ctgagcgaga
     2221 cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc
     2281 aggaacactg ccagcgcatc aacaatattt tcacctgaat caggatattc ttctaatacc
     2341 tggaatgctg tttttccggg gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg
     2401 ataaaatgct tgatggtcgg aagaggcata aattccgtca gccagtttag tctgaccatc
     2461 tcatctgtaa catcattggc aacgctacct ttgccatgtt tcagaaacaa ctctggcgca
     2521 tcgggcttcc catacaagcg atagattgtc gcacctgatt gcccgacatt atcgcgagcc
     2581 catttatacc catataaatc agcatccatg ttggaattta atcgcggcct cgacgtttcc
     2641 cgttgaatat ggctcataac accccttgta ttactgttta tgtaagcaga cagttttatt
     2701 gttcatgatg atatattttt atcttgtgca atgtaacatc agagattttg agacacgggc
     2761 cagagctgcc aggaaacagc tatgaccatg taatacgact cactataggg gatatcagct
     2821 ggatggcaaa taatgatttt attttgactg atagtgacct gttcgttgca acaaattgat
     2881 aagcaatgct ttcttataat gccaactttg tacaagaaag ctgggtctta gccagacaga
     2941 aacagtctct tataggcctt attcatctgc tccagaaaga tgaaggtcag cactgtgtgt
     3001 ggtcccagcc ttgcatagta aggggtaaag cccttccaca gggaaaagaa gccctcgtac
     3061 ctcaccacct tgaacagcac atccaggcca ttcttgtact cgggcttgcc gtcgatcatt
     3121 ctcatgttct gaatccgtgt cttggcgata tccactggca tgcttgctgc ggttgtcacc
     3181 agtccgctga tcatagaggc acagaagtgg cacaggatat tatcagaaaa atagccggag
     3241 tccagcagga actgcttaga ctgggagtag cttgccagct gtgctgcgtt caccaccact
     3301 gcccttgcca tggttgggat gcatcccctc cacagggtca gcacgccctc ctcccttgtg
     3361 atcctgatca gggcattaaa cacgttcttg tagcctctcc gctgatctgc aggcaggcgg
     3421 ccgtctgcgg tcatcctgat cagggccacc tctgctggtg ttcccacgaa ggctccggtt
     3481 gctcctgctg tcatg
//
