LOCUS       sequence-472436-        3974 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3974)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 3974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_HCoV-OC43-nsp15
FEATURES             Location/Qualifiers
     source          1..3974
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(66..83)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(237..825)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(317..336)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(918..1709)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     primer_bind     2140..2156
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2140..2156
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2161..2180
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2161..2179
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2197..2296
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(3481..3580)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(3596..3613)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3596..3612)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3605..3627)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      3676..3762
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3854..3881
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
       61 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
      121 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
      181 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
      241 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
      301 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
      361 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
      421 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
      481 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
      541 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
      601 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
      661 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
      721 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
      781 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
      841 ttttctacgg ggtctgacgc tcagtggaac gacgcgtaac tcacgttaag ggattttggt
      901 catgggtggc tcgagggtta tttgccgact accttggtga tctcgccttt cacgtagtgg
      961 acaaattctt ccaactgatc tgcgcgcgag gccaagcgat cttcttcttg tccaagataa
     1021 gcctgtctag cttcaagtat gacgggctga tactgggccg gcaggcgctc cattgcccag
     1081 tcggcagcga catccttcgg cgcgattttg ccggttactg cgctgtacca aatgcgggac
     1141 aacgtaagca ctacatttcg ctcatcgcca gcccagtcgg gcggcgagtt ccatagcgtt
     1201 aaggtttcat ttagcgcctc aaatagatcc tgttcaggaa ccggatcaaa gagttcctcc
     1261 gccgctggac ctaccaaggc aacgctatgt tctcttgctt ttgtcagcaa gatagccaga
     1321 tcaatgtcga tcgtggctgg ctcgaagata cctgcaagaa tgtcattgcg ctgccattct
     1381 ccaaattgca gttcgcgctt agctggataa cgccacggaa tgatgtcgtc gtgcacaaca
     1441 atggtgactt ctacagcgcg gagaatctcg ctctctccag gggaagccga agtttccaaa
     1501 aggtcgttga tcaaagctcg ccgcgttgtt tcatcaagcc ttacggtcac cgtaaccagc
     1561 aaatcaatat cactgtgtgg cttcaggccg ccatccactg cggagccgta caaatgtacg
     1621 gccagcaacg tcggttcgag atggcgctcg atgacgccaa ctacctctga tagttgagtc
     1681 gatacttcgg cgatcaccgc ttccctcata atgtttaact ttgttttagg gcgactgccc
     1741 tgctgcgtaa catcgttgct gctccataac atcaaacatc gacccacggc gtaacgcgct
     1801 tgctgcttgg atgcccgagg catagactgt accccaaaaa aacagtcata acaagccatg
     1861 aaaaccgcca ctgcgccgtt accaccgctg cgttcggtca aggttctgga ccagttgcgt
     1921 gagcgcatac gctacttgca ttacagctta cgaaccgaac aggcttatgt ccactgggtt
     1981 cgtgccttca tccgtttcca cggtgtgcgt cacccggcaa ccttgggtag cagcgaagtc
     2041 gaggcatttc tgtcctggct ggtctagagg aggcatgatg atatattttt atcttgtgca
     2101 atgtaacatc agagattttg agacacgggc cagagctgcc aggaaacagc tatgaccatg
     2161 taatacgact cactataggg gatatcagct ggatggcaaa taatgatttt attttgactg
     2221 atagtgacct gttcgttgca acaaattgat gagcaattat tttttataat gccaactttg
     2281 tacaagaaag ctgggcccat gaggccgcga tcgcgtctta attaagttgc aaacgaggat
     2341 agaaagtcat aactttctca tcgttacacc aaagcataaa ctgaaaatct ttaaaatcaa
     2401 cattaacatt aacaacctta ctcacacaat taagattaag tgacttaaca agagccacaa
     2461 aatcatccaa caaaatatct ataacagtgc aaacactctt actaccacca ctcttctcgt
     2521 cagtgataaa ataagagtgt atgctggagt catatgaaac aaactcctga acaaccagat
     2581 tggaagtttg ctgtcttcgg tacaagccta ttaacaaatg caaaccacca ataatcttct
     2641 ggttgaagtt accataaaca atgtgttcaa aggcatagtc ctccaaacca tacttctgaa
     2701 taaacacatc ttgatctaaa gctataaaat ctttttccat atcagtacga catgtaaaag
     2761 agctaataac acggctttgt gtaaagatag tagagattga cagagcatca ttaccaccga
     2821 cattaccggg tttaccatta ctccccagat taccttgtgg gctctggtta gagctgactc
     2881 ccaggctgtc gaattggctg aagatgacat cctgaccttc tttacgcaca gcaaaataaa
     2941 acacacaatc agtgtctcca accttgtcca ccactacgcc atttaattca gcacgcggtg
     3001 gacctcttat catcgaaaga ctcttaactt ttgtcgtgga aatgtaaacg ccattattag
     3061 aacgtttaaa agcttcaaga gcaccattat cacgaccatc aaaaaggaca ttcaatttat
     3121 caatgaactt taaatctgta tacatgcaga caccataggt attactgcaa aatatacttt
     3181 ctctagcata atcccaaatg acgtgcttcc aacacacgtc tatatttaaa tttctaaaga
     3241 gcttaagctc tgggtggtgt cgaacactgc gcttggcaaa taattcaacg gccacattag
     3301 tagggtatgt tgtattatta ataaaaatga caacatcctc cttatcgatc ttagccacaa
     3361 ctttatcatt tataatggca caaggcattt caccagcctg tcctgtataa tgaccagtct
     3421 tgactaaatt atatacaaca ttctccaagc tggatcccat ggatcctggc cttgacggcc
     3481 cttctgcttt tttgtacaaa gttggcatta taaaaaagca ttgctcatca atttgttgca
     3541 acgaacaggt cactatcagt caaaataaaa tcattatttg gggcccgagc ttaagactgg
     3601 ccgtcgtttt acaacgtcgt gactgggaaa acatccatgc tagcgttaac gcgagagtag
     3661 ggaactgcca ggcatcaaat aaaacgaaag gctcagtcgg aagactgggc ctttcgtttt
     3721 atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccggg agcggatttg
     3781 aacgttgtga agcaacggcc cggagggtgg cgggcaggac gcccgccata aactgccagg
     3841 catcaaacta agcagaaggc catcctgacg gatggccttt ttgcgtttct acaaactctt
     3901 cctggctagc ggtacgcgta ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     3961 acctgtcgtg ccag
//
