LOCUS       sequence-472469-       10384 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses A. thaliana Cpn60-alpha Cpn60-beta Cpn20 Raf2 RbcX Bsd2 N.
            tabacum Raf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10384)
  AUTHORS   Archer J, Kathpalia M, Lee BU, Li S, Wang T
  TITLE     Effects of chaperone selectivity on the assembly of plant Rubisco 
            orthologs in E. coli.
  JOURNAL   J Exp Bot. 2025 Mar 31:eraf140. doi: 10.1093/jxb/eraf140.
  PUBMED    40163664
REFERENCE   2  (bases 1 to 10384)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Exp Bot. 
            2025 Mar 31:eraf140. doi: 10.1093/jxb/eraf140."
COMMENT     Sequence Label: pTW334i
FEATURES             Location/Qualifiers
     source          1..10384
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             86..877
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        join(1194..1199,1200..1215,1216..1222)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    1230..1246
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             4519..4541
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     RBS             5808..5816
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     RBS             6234..6256
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    7978..7994
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8002..8008,8009..8024,8025..8030))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     promoter        8273..8350
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             8351..9433
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(8370..8389)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     rep_origin      9590..10328
                     /direction=RIGHT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     promoter        join(10378..10384,1..85)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
       61 tcaataatat tgaaaaagga agagtatgag ggaagcggtg atcgccgaag tatcgactca
      121 actatcagag gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca
      181 tttgtacggc tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt
      241 tacggtgacc gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga
      301 aacttcggct tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt
      361 gcacgacgac atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg
      421 gcagcgcaat gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc
      481 tatcttgctg acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga
      541 actctttgat ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct
      601 atggaactcg ccgcccgact gggctggaga tgagcgaaat gtagtgctta cgttgtcccg
      661 catttggtac agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc
      721 aatggagcgc ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct
      781 tggacaagaa gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta
      841 cgtgaaaggc gagatcacca aggtagtcgg caaataaacg ccatgggcat taaaaaatgc
      901 cctcttgggt tatcaagagg gtcattatat ttgctgagga gtgccgttaa tgcgcaacgc
      961 aattaatgta agttagctca ctcattaggc acaattctca tgtttgacag cttatcatcg
     1021 actgcacggt gcaccaatgc ttctggcgtc aggcagccat cggaagctgt ggtatggctg
     1081 tgcaggtcgt aaatcactgc ataattcgtg tcgctcaagg cgcactcccg ttctggataa
     1141 tgttttttgc gccgacatca taacggttct ggcaaatatt ctgaaatgag ctgttgacaa
     1201 ttaatcatcg gctcgtataa tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
     1261 cagccagtcc gtttaggtgt tttcacgagc acttcaccaa caacgcgtac aactaggaga
     1321 tataccatgg gtatgcagca gctgtatcag ccgtttcgtc ctccaccacc gctgttacct
     1381 ccgaaatttc ggaatttaga taccaacagc aagttggaag tgcttgcaaa tcgcctgggt
     1441 ctttggtatg aatacgcccc actgattccc agcctaaccc gcgaaggctt taccccgtct
     1501 acgctggagg aaattactgg cctgacggga gtggatcaaa atcgtttggt ggtggcggcc
     1561 caggttcgag attcgctggt agaaagcgcg gcgcttgacg aggagacgct gagctatttt
     1621 gagtcagggg gtgcggaact gctgtatgaa atccgcctgc tctccgcccg acagcgcgct
     1681 gatgccgcta catttctcgt caaaaacgga ttcgacgcca aacaagcgca agatttagct
     1741 cgtgccatta aagattatcc caggcgtcgt gtggagtacg gctgggataa attccatggc
     1801 gactcacctg gcgattgcct cgcgttcatg tatttccgct tggcacagga atacgcagca
     1861 gccgcgagtg aagacctgcg gcgctcgagc atggaaaaag ccctggaagt agcggaatcg
     1921 gaaagtgcgc gcaatttatt agtgatggag ctggaaggca atgaagtcgc gaaagaatcc
     1981 gttaaagatg acggcgttac cgttccgctg gtccgtatga aactgggtga ggtcgcggaa
     2041 agcaccatag ttgtagttct cccggtatgt aaggcggaag gccgtgatgt agaagttgag
     2101 gcggctccgt gggaatgcgg aggcgttggt gatttcggga ttgtggaagc cgaaaaagac
     2161 tggcgccgtt gggtggtact tccggggtgg cagcctatcg caggtttgga acgcggtggt
     2221 gtggccgtat catttaaatc tggcaacttt ctgccgtggc gtgaaaagtc gaaatacaag
     2281 caggaacccg tgctggtcgt ggcagatcgc ggccgtaccg aagttgcaag tgaggatggt
     2341 ttctacttgg tcgtggatgg gggcaacggc agcaacgaag agggactaaa ggtggaacgc
     2401 ggttccactt taaaaaaacg cggtgtcgaa cagagcctgg gcatcgtgct tattgttgtg
     2461 agaccgccga aatgggagaa cgaggaccaa ctgggcgaag aggactggga ttaaattaca
     2521 aaaattagga gatatacata tgatgtctaa tctggcgcag gattttcttg gtgacttcgg
     2581 agctcgtgac ccttacccgg aggagatagc gagtcaattc ggagataaag tgttgggatg
     2641 ccaaagcact gagcacaaga ttttgatacc aaacgcatct gttttgtctc tctcccagct
     2701 tcagtgttcc cctgtttcgt cttcacagcc tcctttgtcc ggcgatgatg ccagaactct
     2761 cctccacaag gttttgggat ggagtatagt ggataatgaa gcgggtggtc tgaaaataag
     2821 gtgtatgtgg aaagtgaggg attttgggtg cggtgttgaa ctcataaaca ggatccataa
     2881 ggttgctgaa gcttctggtc attacccttc tcttcacttg gaaagtccta cccaagttcg
     2941 agctgaacta tttacctctt ctatcggagg gttgagcatg aatgatttca taatggcggc
     3001 taaaatagat gatatcaaga cttctgatct ttcccctagg aaaagagctt gggcgtgacg
     3061 gctgaaatta atgaggagat atacatatgg tatcgagaat tgcgaatcga aagagcaaaa
     3121 agctcttgat tgtgaatgag gatgttgctg gtaattacga cgataccttc ggtgatgtac
     3181 aaaagcaaat tgttaattat tttacataca aagctgtgag gacggttctt catcagctgt
     3241 acgaaatgaa tcctcctcaa tacacttggt tctataatca tatcataaca aacagaccga
     3301 cggatggcaa acgtttcctc cgtgccctcg gcaaggagag tcaggagctt gcagaaagag
     3361 tgatgatcac gcgtcttcac ttgtatggca aatggatcaa gaaatgcgac catgggaaga
     3421 tataccagga aatatcggat gaaaacttgg cgttgatgcg tgaacgcctg atggagacag
     3481 tgatatggcc ttccgatgac acaaactcaa ggtaattcag ctaagacact gcacaggaga
     3541 tatacatatg atggccgcaa acaataatcc tcagggcact aaaccaaaca gtttagtttg
     3601 tgcaaattgt gaaggggaag gttgtgtggc atgttctcaa tgcaaaggag gtggtgtgaa
     3661 tttgattgat catttcaatg gtcagtttaa agctggtgca ctctgttggc tttgcagagg
     3721 taaaaaggag gtgttgtgtg gggactgcaa tggagctggt ttcattggtg ggttcttgag
     3781 caccttcgat gagtagagtc agtagggccc taaaaaaaaa ccccgcccct gacagggcag
     3841 ggtttttttt ggcgaaaaaa ccccgctgaa gcggggtttt ttgcgtacat tggctccgtc
     3901 taagaaagta tagccatcac atctgaagct ctgagggcaa tgtagttgga accatctttg
     3961 cccttgaagt cgttaccagc atacttggag taaagtactg tgcttccggt tgatactggt
     4021 agaggcgtaa ttttaccttc ctcgtctagg gaacccggtc caactgctat cacggtgcca
     4081 atagaaggct tctctttggt agtctcggtt aacaacaacc ctccagctgt tttctcctcc
     4141 gcctcagcaa ccttaataaa gactcggtca ttcaaaggtt tgagatcttt gatgtcctct
     4201 gtctcaagaa tgccaacaat atcatcttcc ttgaggataa gatgcttcac atcattgaac
     4261 tccacctcag ttcctgcgta tttggagtag ataatttgtg ctccagtagg gacagtgata
     4321 tcaattttgt tcttcccaat agttcttcct tcacccacag caacgacttc acctccttga
     4381 ggttttgatt gagcagtgga tggaagtaag ataccaccta aagtcttctc ctctgcctcc
     4441 ttgatcttca ccaaaactcg atctcccaat ggcttaattg aagtatactt aggggcaaca
     4501 acagaggcca tatgtatatc tccttcttaa agttaaacaa aattatttct agagggcaat
     4561 tgttatccgg tcgacgtcga ccttagtatc cgtaacctga gttgtccatt gggttgccga
     4621 cgggaactgg ttcaggttcc ttgatctcaa caacaacgca gtccgacatc aagaaagtct
     4681 ttgcaaccga agctgcgtgt tccaagcaac atctcacaac ctttgttgga tcgatgattc
     4741 ctgcagccat caaatcctcg tacttgccgg ttgcagcatt gtaaccgaat ttcacgttat
     4801 cgttggaaag caccttctcg ctaactacac ttccattaac accagcattc ttggcgatta
     4861 gttttagggg gtaacttagt gctcttttaa ctatatccgc tccaaccttt tcttcatcat
     4921 tatcaagcgt ggctttaatg gcatcaacct tggaagcaag acgaagcaga gtgcaaccac
     4981 caccaacaac aataccttcc tcgacagcgg cctttgttgc attaagagca tcttcaactc
     5041 tcaacttctt ctctttgagt tctgtctcag tttgtgctcc aacctgaatc acagcaactc
     5101 caccagagag ctttgcaatt ctctcattga gtttctcctt ctcgtaatct tgctccgctt
     5161 gctcaataag atttttaatc tgtgtaacac gctttttcac tgcatcctgt gtgcttccat
     5221 cacccactat ggtcgaggtt tcctttgtga ggacaacctt tgaagcatta ccaagaacct
     5281 cttttccagc tttgtcaagt gaaagaccaa cttcctcacg gatcacagtt gctccagtta
     5341 gaatggcaat atcatcaagg tattggctct tgcgctctcc aaaccctgga gctctgagag
     5401 ctgctatctt cagtgtacct ctaagcttgt ttacaacaag agtcgctaaa gcttcttgtt
     5461 caatgtcttc agcaataatt aatattgggt atccgcctct aattgcatcc tccaaaacac
     5521 caacaagatc ccttgcattg gtaattttct tgtcaacgag aagtaacttg caattgtcga
     5581 actcgacgga cattttctcg ctgtctgtca caaagtaagg agagatataa ccacggtcaa
     5641 attgcattcc ttctacgacg tagaggttgt tctcggcact tttaccctcc tcaagggtaa
     5701 ccacaccttt cctgcccact ttgctcattg cttcagcaat catatttcca atttcgtcgt
     5761 tgttacccgc actaacagct gcaacatctg caagttcact gtcttcaacc tccttagaca
     5821 ttttcttgag ctcggtaacc aaagcctttg ctgtcttctc aatacctctg gtgatcaaaa
     5881 cagggtttgc accagcagct accaccttga caccctcagc gataaaacct tgtgcaagaa
     5941 caacagatgt tgttgtaccg tcaccagcca agtcattggt tttggcagcc gcttgcctca
     6001 caagcttagc gccaatgttc tcaaccgggt cttccaactc aacctctctt gcaacagtca
     6061 caccatcatt aacaattcgt ggtgatccat acttgctctc aagaacaaca tttcgtcctt
     6121 tgggtccaag tgtaacgcca acaagatctg cgagcttgtt gacaccagct tgaagtctcc
     6181 taatggtagt cccatctttg ttgaaatgta attcctttgc tgccatatgt atatctcctt
     6241 cttaaagtta aacaaaatta tttctagctc cttacaccat gaggccctca ggagcagcag
     6301 cagcaggagc cttgggtttc ggtttgtcaa caacaatggc ctgagtggtc agtaccattc
     6361 ctgctacgga agcagcgttc tgtagcgcac atcttgtcac tttagccgga tcaatcactc
     6421 cagcttcgaa cagattctcg taagtatcag tcattgcgtt gtacccattc tcccaatcgc
     6481 tgaacataat cttttccaca acaacttctc cttcaactcc agcgttctga gcaataagag
     6541 cagctggtga cagcaatgcc ttttgtacta tgtcagctcc caaacgttca tcagcatcct
     6601 caaaagtctc cttaatggcg ggaataacag tggagagatg caccaaagca gcaccaccac
     6661 ctggaactat gccttcctcg atagcagcaa atgttgcgtt ctttgcatcc tcgatacgaa
     6721 gcttacggtc ctcaagctca gtttcagttg ctgctccgac tttaatgaca gcaacacctc
     6781 cagatagctt cgcaattctc tcagcgagct tctctgagtc atacacagaa tcagtctcaa
     6841 atagttcttt cttcagctga gcaatccgag cttgtaattc gtccttggaa gctgcatctg
     6901 caatgagggt agtcgaatct ttgctaatcg tgacttttcg ggcaataccc aattgatcta
     6961 tggttgcgtt ttcaaccagt aagctcatgt ccatggctag gtactcagct cctgtcaaga
     7021 ttgcaatatc ttgaagcatg gcttttcttc tttctccaaa tcctggcgct ttaacggcaa
     7081 caacattgag gacaccacga agcttgttca cgacaagggt agctaaggct tcaccagtaa
     7141 catcctctgc aataatcaac aacggagcac gaagctgagt ggtcttctcc aaaataggga
     7201 tgatgtcttt gatcgcagtg atcttctgat cagtgatcaa taccctagca ttctcaaact
     7261 cagctagtag tttctcaggg tttgtaacaa actgaggcga gatgtaacct ctgtcaatct
     7321 ccatcccttc ttcaacttcg accgtggttt caaaagaaga tgaagattca atggacaaaa
     7381 caccatcagg tccaactttg tcgatggcat cagcaatcat tgaccctata aggtcatcat
     7441 ttccagcaga gatagaagcc acagctctga tgtcatcacg acctttcacg ggcctagctt
     7501 tcttctgaag ctcttcgatc agaccttgaa cagtcttatc aattcccctc ttgagtgaaa
     7561 cgggattcgc accagaagtg acgctcaaca atccatgttt gattatctcc cgagcaagga
     7621 tagacgcagt ggttgtccca tcaccagcag agtcattagt cttactcgca acctcacgaa
     7681 taagcgctgc accagcattt tccatggcat taggtaactc aatagctcta gcaatggtga
     7741 ctccatcatt cacaacctta ggacttccaa attcatccaa cacaacattc ctccctctgg
     7801 ggccaagcgt aagaccaaca caatcagcaa gcttatcaat accagcttgt agagcagctc
     7861 tagagtgctg gtcaaaagct atttccttta cattcatatg tatatctcct actgcaccta
     7921 ttgttggtga agtgctcgtg aaaacaccta aacggactgg ctgtttcctg tgtgaaattg
     7981 ttatccgctc acaattccac acattatacg agccgatgat taattgtcaa cagctcattt
     8041 cagaatattt gccagaaccg ttatgatgtc ggcgcaaaaa acattatcca gaacgggagt
     8101 gcgccttgag cgacacgaat tatgcagtga tttacgacct gcacagccat accacagctt
     8161 ccgatggctg cctgacgcca gaagcattgg tgcaccgtgc agtcgatgat aagctgtcaa
     8221 acatgagaat tgtgcctaat gagtgagcta acttacatta attgcgttgc gcgacaccat
     8281 cgaatggtgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga gtcaattcag
     8341 ggtggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta
     8401 tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa
     8461 agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc
     8521 gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc
     8581 gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc
     8641 gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca
     8701 acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga
     8761 agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa
     8821 cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt
     8881 gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg
     8941 tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga
     9001 aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat
     9061 cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat
     9121 taccgagtcc gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga
     9181 agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg
     9241 gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca
     9301 gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc
     9361 ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga
     9421 aagcgggcag tgaagggtcc ctaagtctcc tcagcaaaac gaaaggccca gtctttcgac
     9481 tgagcctttc gttttatttg accggatgtc ctcttcctcc tgggctgact tcaggtgcta
     9541 catttgaaga gataaattgc actgaaatct agagcggttc agtagaaaag atcaaaggat
     9601 cttcttgaga tccttttttt ctgcgcgtaa tcttttgccc tgtaaacgaa aaaaccacct
     9661 ggggaggtgg tttgatcgaa ggttaagtca gttggggaac tgcttaaccg tggtaactgg
     9721 ctttcgcaga gcacagcaac caaatctgtc cttccagtgt agccggactt tggcgcacac
     9781 ttcaagagca accgcgtgtt tagctaaaca aatcctctgc gaactcccag ttaccaatgg
     9841 ctgctgccag tggcgtttta ccgtgctttt ccgggttgga ctcaagtgaa cagttaccgg
     9901 ataaggcgca gcagtcgggc tgaacgggga gttcttgctt acagcccagc ttggagcgaa
     9961 cgacctacac cgagccgaga taccagtgtg tgagctatga gaaagcgcca cacttcccgt
    10021 aagggagaaa ggcggaacag gtatccggta aacggcaggg tcggaacagg agagcgcaag
    10081 agggagcgac ccgccggaaa cggtggggat ctttaagtcc tgtcgggttt cgcccgtact
    10141 gtcagattca tggttgagcc tcacggctcc cacagatgca ccggaaaagc gtctgtttat
    10201 gtgaactctg gcaggagggc ggagcctatg gaaaaacgcc accggcgcgg ccctgctgtt
    10261 ttgcctcaca tgttagtccc ctgcttatcc acggaatctg tgggtaactt tgtatgtgtc
    10321 cgcagcgccc gccgcagtct cacgcccgga gcgtagcgac cgagtgacgg ccttaatttg
    10381 gctt
//
