LOCUS       sequence-472632-       11267 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protin expression of FL yeast VPS15 -ZZ in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11267)
  AUTHORS   Rostislavleva K, Soler N, Ohashi Y, Zhang L, Pardon E, Burke JE, 
            Masson GR, Johnson C, Steyaert J, Ktistakis NT, Williams RL
  TITLE     Structure and flexibility of the endosomal Vps34 complex reveals the
            basis of its function on membranes.
  JOURNAL   Science. 2015 Oct 9;350(6257):aac7365. doi: 10.1126/science.aac7365.
  PUBMED    26450213
REFERENCE   2  (bases 1 to 11267)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2015-10-9"; volume: "350(6257):aac7365. doi"; pages: " 
            10.1126/science.aac7365"
COMMENT     Sequence Label: pYO225
FEATURES             Location/Qualifiers
     source          1..11267
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        194..409
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             410..1213
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     rep_origin      complement(1344..1799)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1481..1502)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     1693..1712
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1925..1947
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1939..1956
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1940..1956
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1966..1985
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1966..1984
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2068..2185
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     2366..2389
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     CDS             6889..6909
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             6931..6951
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             7033..7053
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             7069..7242
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             7243..7416
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     terminator      7439..7626
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(7494..7518)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(7665..7685)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(7665..7683)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7704..7720)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7704..7720)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7717..7739)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7728..7744
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7752..7758,7759..7776,7777..7782))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7797..7818
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7935..7952)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8106..8694)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8186..8205)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8865..9656,9657..9725))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9488..9507
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9726..9830)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      9857..11199
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     protein_bind    complement(10820..10867)
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     11240..11258
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accataccac cttttcaatt catcattttt tttttattct tttttttgat ttcggtttcc
      241 ttgaaatttt tttgattcgg taatctccga acagaaggaa gaacgaagga aggagcacag
      301 acttagattg gtatatatac gcatatgtag tgttgaagaa acatgaaatt gcccagtatt
      361 cttaacccaa ctgcacagaa caaaaacctg caggaaacga agataaatca tgtcgaaagc
      421 tacatataag gaacgtgctg ctactcatcc tagtcctgtt gctgccaagc tatttaatat
      481 catgcacgaa aagcaaacaa acttgtgtgc ttcattggat gttcgtacca ccaaggaatt
      541 actggagtta gttgaagcat taggtcccaa aatttgttta ctaaaaacac atgtggatat
      601 cttgactgat ttttccatgg agggcacagt taagccgcta aaggcattat ccgccaagta
      661 caatttttta ctcttcgaag acagaaaatt tgctgacatt ggtaatacag tcaaattgca
      721 gtactctgcg ggtgtataca gaatagcaga atgggcagac attacgaatg cacacggtgt
      781 ggtgggccca ggtattgtta gcggtttgaa gcaggcggca gaagaagtaa caaaggaacc
      841 tagaggcctt ttgatgttag cagaattgtc atgcaagggc tccctatcta ctggagaata
      901 tactaagggt actgttgaca ttgcgaagag cgacaaagat tttgttatcg gctttattgc
      961 tcaaagagac atgggtggaa gagatgaagg ttacgattgg ttgattatga cacccggtgt
     1021 gggtttagat gacaagggag acgcattggg tcaacagtat agaaccgtgg atgatgtggt
     1081 ctctacagga tctgacatta ttattgttgg aagaggacta tttgcaaagg gaagggatgc
     1141 taaggtagag ggtgaacgtt acagaaaagc aggctgggaa gcatatttga gaagatgcgg
     1201 ccagcaaaac taaaaaactg tattataagt aaatgcatgt atactaaact cacaaattag
     1261 agcttcaatt taattatatc agttattacc ctatgcggtg tgaaataccg cacagatgcg
     1321 taaggagaaa ataccgcatc aggaaattgt aagcgttaat attttgttaa aattcgcgtt
     1381 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     1441 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     1501 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     1561 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     1621 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     1681 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     1741 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     1801 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     1861 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
     1921 ttttcccagt cacgacgttg taaaacgacg gccagtgagc gcgcgtaata cgactcacta
     1981 tagggcgaat tgggtaccgc tatgacatga ttacgaattg gcgcgccggc cgcggatctt
     2041 tgcaaagatc caaaaaaagc atgcgtacgg attagaagcc gccgagcggg tgacagccct
     2101 ccgaaggaag actctcctcc gtgcgtcctc gtcttcaccg gtcgcgttcc tgaaacgcag
     2161 atgtgcctcg cgccgcactg ctccgaacaa taaagattct acaatactag cttttatggt
     2221 tatgaagagg aaaattggca gtaacctggc cccacaaacc tcaaatgaac gaatcaaatt
     2281 aacaaccaga tatctcgact gaaaaaaaag gtttaaacca gttccctgaa attattcccc
     2341 tacttgacta ataagtatat aaagacggta ggtattgatt gtaattctgt aaatctattt
     2401 cttaaacttc ttgaattcta cttttatagt tagtcttttt tttagtttta aaacaccaag
     2461 aacttagttt cgaataaaca cacataaata aacctcgaga tgggggcaca attatcacta
     2521 gtggtccaag catcaccttc catagccatt ttttcatata tcgatgtctt agaggaagta
     2581 cactacgttt cacagttaaa ctcatcaaga ttcttaaaaa catgcaaggc actggaccct
     2641 aacggcgaaa ttgttatcaa agtgtttatt aaaccaaaag accaatatag cttacgacct
     2701 tttctccaac gtataagggc tcaatcgttt aagttgggac aactaccgca cgttttaaac
     2761 tacagtaaat tgatcgagac aaatagagcc ggctacatga tacggcagca cttaaaaaat
     2821 aatttatatg acagattgag tttgagacct tacttacaag acattgaact gaaattcatt
     2881 gctttccagt tgttaaatgc attaaaggac attcataatc tgaatattgt ccatggtgat
     2941 ataaagacag aaaatatcct agtaacaagt tggaattggt gtatattgac agattttgct
     3001 gcatttatca aacccgtata tttgcctgaa gataatccag gtgaattttt attctacttc
     3061 gacacctcga agagaagaac ctgttatcta gccccggaga ggtttaactc taaactttac
     3121 caagatggaa aatctaacaa tggtaggcta actaaagaaa tggacatatt tagtcttgga
     3181 tgtgttattg cagaaatatt tgctgaagga agacccatct tcaacttatc acagctattc
     3241 aaatataaaa gtaattcata tgacgtaaac agggaatttc tcatggagga aatgaattct
     3301 accgatttaa ggaacttggt tctagacatg attcaactag atccatccaa aagactttca
     3361 tgtgatgaac tactgaataa atatcgtggc attttcttcc ccgattattt ctacactttc
     3421 atttatgatt atttcagaaa tttggttact atgacaacaa gcacaccgat atcagataac
     3481 acttgcacca atagtacctt ggaagacaat gtaaaacttt tagatgaaac tacggaaaaa
     3541 atatacagag atttttccca aatatgtcat tgtttggact ttcctttaat aaaagacggg
     3601 ggtgagattg gttcagaccc cccaattttg gaatcttaca aaatagagat agaaattagt
     3661 cggtttttaa acacaaactt atatttcccc caaaattacc atttagtctt acagcagttt
     3721 accaaagtat ccgaaaagat aaaatcagtt aaagaggaat gtgccttact ctttatctct
     3781 tatttgtctc atagtataag aagtattgtt tcgactgcta cgaaacttaa aaatttagaa
     3841 ctattagcag tatttgcaca attcgtttct gatgaaaata aaattgatcg agtcgtacct
     3901 tatttcgtat gttgttttga agatagtgac caggacgtcc aggccctatc tttgttaaca
     3961 ttaatccagg tactcacctc tgtaagaaaa ttgaatcaat tgaacgagaa tatatttgtg
     4021 gactacttac ttccgagact gaaaagatta cttatttcca ataggcagaa taccaattat
     4081 ttaaggattg tgtttgctaa ttgtttgagc gacttggcca ttatcattaa tagatttcaa
     4141 gaatttacat ttgctcagca ctgcaatgat aactcaatgg ataacaacac ggaaatcatg
     4201 gaaagcagta ccaagtattc agcaaaattg atccaaagtg tcgaagattt aactgtctct
     4261 tttttaacag ataatgatac ttatgtaaag atggcacttt tgcaaaacat tcttccactt
     4321 tgtaaatttt ttggtaggga aagaacaaac gatattatac tgagtcattt aataacctac
     4381 cttaatgata aggacccagc attgcgagtt tccttaattc aaacaatatc cggaatatca
     4441 attcttttgg gtaccgttac attagaacag tatattttac cattgttaat ccagaccatc
     4501 actgattcgg aagaattagt agtgatcagt gttttacaaa gcttaaaatc tttgttcaag
     4561 actgggttga ttaggaaaaa atattatatt gatatatcaa aaacaacatc tcccttgttg
     4621 ttgcatccta ataattggat aagacagttt actttgatga taattataga aattattaat
     4681 aagttatcaa aagccgaagt gtactgcatt ctctatccaa taataaggcc tttcttcgaa
     4741 tttgacgttg agttcaactt caaatcaatg ataagctgtt gcaagcaacc agtgtcaaga
     4801 tcggtttaca atctattgtg tagttggtct gttagagcgt caaaatcttt attttggaaa
     4861 aaaatcatca caaatcatgt agattcattc ggaaataata gaatcgaatt tataacaaaa
     4921 aattactcaa gtaaaaatta tggatttaat aaaagagata cgaaatcaag ttcctcgctg
     4981 aagggtatta aaacatcatc caccgtctat tcacatgaca acaaggaaat tcccttaact
     5041 gctgaagaca gaaattggat tgataagttc cacattattg ggctaacaga aaaagatatt
     5101 tggaaaattg tggctttgag gggttatgta ataaggacag cgagagttat ggcagcgaac
     5161 cctgattttc catataataa tagtaattac cgtccattag tacagaactc accacctaac
     5221 ctaaacctta cgaatattat gccaagaaac attttctttg atgtagagtt tgctgaagag
     5281 tcgacaagtg agggacaaga ttctaactta gagaaccaac agatatataa atatgatgaa
     5341 agtgagaaag atagcaataa gctaaatatt aacggtagca aacagctatc taccgtcatg
     5401 gacataaatg gatcactaat attcaaaaat aagtccattg ccactactac ttctaatttg
     5461 aagaacgttt ttgttcagtt agaaccaacg tcctatcaca tgcattctcc aaatcatggc
     5521 ttgaaagata atgcaaatgt taaaccagaa aggaaggtag tcgtcagcaa cagctatgaa
     5581 ggcgacgttg aaagcataga aaaattccta tcgactttca aaattttacc tcctctgaga
     5641 gattataagg agtttgggcc tattcaagag attgtacgga gtccaaacat gggtaatttg
     5701 aggggcaagt tgatagctac tttgatggaa aacgaaccca attctattac gtcttctgct
     5761 gtttctccag gagaaacacc ctatttaata acaggttcag atcaaggtgt aatcaagatt
     5821 tggaacctga aagagattat cgtgggcgag gtttactctt cttctttaac ttatgactgc
     5881 tcctctaccg taactcagat aaccatgatt cctaactttg acgcgtttgc cgtttccagt
     5941 aaagatggac aaataattgt attaaaggtt aatcattacc aacaagaaag tgaagtcaaa
     6001 tttttgaatt gcgaatgcat caggaaaatt aacttgaaga attttggtaa aaatgaatac
     6061 gcagtgagaa tgagagcatt tgtgaatgag gaaaaatctc tactagtagc attgacgaat
     6121 ttgtcaaggg ttattatatt tgatattaga accctggaga ggttacaaat tatagagaat
     6181 tctccaaggc atggtgccgt ttcaagcatc tgtatcgatg aagagtgttg tgtcctaatt
     6241 ttggggacga ctagaggtat tattgatata tgggatatcc gtttcaacgt gctgataagg
     6301 agttggtcct ttggggacca cgcaccaatc acgcatgtgg aggtttgtca gttttatgga
     6361 aagaattctg taattgttgt aggaggtagt tcaaaaacat ttctaacaat atggaatttt
     6421 gttaaggggc attgtcagta tgctttcata aattctgatg aacagccatc tatggagcac
     6481 tttttaccaa ttgagaaagg cttagaagaa ttaaattttt gtggaatcag gtctttaaac
     6541 gcactaagca ctatctcagt atctaatgat aaaattcttc ttaccgatga agcaacaagt
     6601 tccattgtta tgtttagcct aaatgagctt tcttcttcta aagcagtaat aagtccttca
     6661 agattcagtg acgtttttat tcctacacaa gttacggcaa atctcacaat gttattgaga
     6721 aaaatgaaac gtactagcac tcattcagta gatgattctc tatatcatca tgatattata
     6781 aattctatat ctacatgtga agttgatgag acacctttgc tggttgcttg tgataactca
     6841 gggcttattg gaatcttcca aggatcctct agaccaacta ctgcctctga aaatttgtac
     6901 tttcaaggcg agttgaagac cactgcttct gaaaacttgt acttccaagg tgagttactc
     6961 gagggtgaat tgaagactgc tgctttggct caacacgacg aagctagatc cggagcaggg
     7021 gcgggtgcta gcgagaacct gtacttccag ggcgcccacg atgaagccgt agacaacaaa
     7081 ttcaacaaag aacaacaaaa cgcgttctat gagatcttac atttacctaa cttaaacgaa
     7141 gaacaacgaa acgccttcat ccaaagttta aaagatgacc caagccaaag cgctaacctt
     7201 ttagcagaag ctaaaaagct aaatgatgct caggcgccga aagtagacaa caaattcaac
     7261 aaagaacaac aaaacgcgtt ctatgagatc ttacatttac ctaacttaaa cgaagaacaa
     7321 cgaaacgcct tcatccaaag tttaaaagat gacccaagcc aaagcgctaa ccttttagca
     7381 gaagctaaaa agctaaatga tgctcaggcg ccgaaagtag acgcgaattc gtgagctcgc
     7441 gaatttctta tgatttatga tttttattat taaataagtt ataaaaaaaa taagtgtata
     7501 caaattttaa agtgactctt aggttttaaa acgaaaattc ttattcttga gtaactcttt
     7561 cctgtaggtc aggttgcttt ctcaggtata gcatgaggtc gctcttattg accacacctc
     7621 taccggcagc ggccgccacc gcggtggagc tccagctttt gttcccttta gtgagggtta
     7681 attgcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     7741 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     7801 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     7861 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     7921 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     7981 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     8041 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     8101 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     8161 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     8221 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     8281 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     8341 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     8401 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     8461 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     8521 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     8581 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     8641 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     8701 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     8761 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     8821 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     8881 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     8941 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     9001 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     9061 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     9121 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     9181 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     9241 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     9301 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     9361 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     9421 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     9481 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     9541 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     9601 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     9661 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     9721 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     9781 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     9841 cgaaaagtgc cacctgaacg aagcatctgt gcttcatttt gtagaacaaa aatgcaacgc
     9901 gagagcgcta atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa
     9961 cgcgaaagcg ctattttacc aacgaagaat ctgtgcttca tttttgtaaa acaaaaatgc
    10021 aacgcgagag cgctaatttt tcaaacaaag aatctgagct gcatttttac agaacagaaa
    10081 tgcaacgcga gagcgctatt ttaccaacaa agaatctata cttctttttt gttctacaaa
    10141 aatgcatccc gagagcgcta tttttctaac aaagcatctt agattacttt ttttctcctt
    10201 tgtgcgctct ataatgcagt ctcttgataa ctttttgcac tgtaggtccg ttaaggttag
    10261 aagaaggcta ctttggtgtc tattttctct tccataaaaa aagcctgact ccacttcccg
    10321 cgtttactga ttactagcga agctgcgggt gcattttttc aagataaagg catccccgat
    10381 tatattctat accgatgtgg attgcgcata ctttgtgaac agaaagtgat agcgttgatg
    10441 attcttcatt ggtcagaaaa ttatgaacgg tttcttctat tttgtctcta tatactacgt
    10501 ataggaaatg tttacatttt cgtattgttt tcgattcact ctatgaatag ttcttactac
    10561 aatttttttg tctaaagagt aatactagag ataaacataa aaaatgtaga ggtcgagttt
    10621 agatgcaagt tcaaggagcg aaaggtggat gggtaggtta tatagggata tagcacagag
    10681 atatatagca aagagatact tttgagcaat gtttgtggaa gcggtattcg caatatttta
    10741 gtagctcgtt acagtccggt gcgtttttgg ttttttgaaa gtgcgtcttc agagcgcttt
    10801 tggttttcaa aagcgctctg aagttcctat actttctaga gaataggaac ttcggaatag
    10861 gaacttcaaa gcgtttccga aaacgagcgc ttccgaaaat gcaacgcgag ctgcgcacat
    10921 acagctcact gttcacgtcg cacctatatc tgcgtgttgc ctgtatatat atatacatga
    10981 gaagaacggc atagtgcgtg tttatgctta aatgcgtact tatatgcgtc tatttatgta
    11041 ggatgaaagg tagtctagta cctcctgtga tattatccca ttccatgcgg ggtatcgtat
    11101 gcttccttca gcactaccct ttagctgttc tatatgctgc cactcctcaa ttggattagt
    11161 ctcatccttc aatgctatca tttcctttga tattggatca tactaagaaa ccattattat
    11221 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtc
//
