LOCUS       sequence-47279-e         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  TITLE     Seifert lab unpublished plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pGCC5
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    215..248
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc
       61 cggtagtgat tttatttcat tatggtgaaa gttggaacct cttaccgata agcttctaga
      121 gatcttccat acctaccagt tctgcgcctg cagcaatggc aacaacgttg cccggatccg
      181 cggccaattc cgatcatatt caataaccct taatataact tcgtataatg tatgctatac
      241 gaagttatta ggtctgaaga ggagtttacg tccagccaag cttgccgtct gaaatggttt
      301 cagacggcat gcaggtcgac taggacgtct ccggattaat taagtactat gcatgtttaa
      361 acggccggcc ctagtgctag cggatccccc ttaacgtgag ttttcgttcc actgagcgtc
      421 agaccccgaa acgagggtat agagcagaac ggatggttct tgttggcgga tgtcttcagg
      481 aagggtaagc gcagtcatgg tatgccgtct gaaaagtggg gattatagcg gattgcggct
      541 ttgcgccgaa aatatccttt agcctgccga tggcgtaaaa tgggcgcacg ccaaccacgc
      601 aaaggaaaat caaatggaca atctgaatcc gcaggaaatt tccgtgttgc cggaaaatct
      661 gccgctgtat tgctcgggac cggacaacga gcagtggaac gggcatccga gggtgttttt
      721 acctttgggc gaaggagaat cgggcagcgt tgcctgcccg tattgcggca cgcgctaccg
//
