LOCUS       sequence-47319-e         788 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Adamson DN, Lim HN
  TITLE     Rapid and robust signaling in the CsrA cascade via RNA-protein 
            interactions and feedback regulation.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Aug 6;110(32):13120-5. doi: 
            10.1073/pnas.1308476110. Epub 2013 Jul 22.
  PUBMED    23878244
REFERENCE   2  (bases 1 to 788)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1308476110"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2013-08-6- 6"; volume: "110"; issue: "32"; pages: "13120-5"
COMMENT     Sequence Label: pHL600
FEATURES             Location/Qualifiers
     source          1..788
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     79..97
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     protein_bind    118..136
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    143..161
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      560..631
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    701..717
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
       61 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca cctcgagtcc
      121 ctatcagtga tagagattga catccctatc agtgatagag atactgtcga cgagtcagac
      181 aacgaagtga acatcaggat gatgacactt ctgcaggaca caccaggatg gtgtttcagg
      241 gaaaggcttc tggatgaagc gaagaggatg acgcaggacg cgttaaagga cacctccagg
      301 atggagaatg agaaccggtc aggatgattc ggtgggtcag gaaggccagg gacacttcag
      361 gatgaagtat cacatcgggg tggtgtgagc aggaagcaat agttcaggat gaacgattgg
      421 ccgcaaggcc agaggaaaag ttgtcaagga tgagcaggga gcaacaaaag tagctggaat
      481 gctgcgaaac gaaccgggag cgctgtgaat acagtgctcc ctttttttat tgggcccatg
      541 gtacgcgtgc tagaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
      601 ttttatctgt tgtttgtcgg tgaacgctct cnnnnnnngg acaaatccgc cncnnnnnac
      661 ctangcgcgg atccataaat gtgancggat ancattgaca ttgtgagcgg ataacaagat
      721 actcccggnn anngangaaa anaaaatgct gattctnacn cnnnnagttg gngagaccct
      781 cangantg
//
