LOCUS       sequence-47339-e         928 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Shcherbakova DM, Hink MA, Joosen L, Gadella TW, Verkhusha VV
  TITLE     An Orange Fluorescent Protein with a Large Stokes Shift for 
            Single-Excitation Multicolor FCCS and FRET Imaging.
  JOURNAL   J Am Chem Soc. 2012 May 9;134(18):7913-23. doi: 10.1021/ja3018972
  PUBMED    22486524
REFERENCE   2  (bases 1 to 928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "J Am Chem Soc"; date: "2012-05-9- 9"; volume: "134"; issue: "18"; 
            pages: "7913-23"
COMMENT     Sequence Label: pLSSmOrange-C1
FEATURES             Location/Qualifiers
     source          1..928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    131..196
                     /label=MCS
                     /note="multiple cloning site"
     CDS             complement(197..904)
                     /codon_start=1
                     /product="monomeric orange fluorescent protein with a large
                     Stokes shift (Shcherbakova et al., 2012)"
                     /label=LSSmOrange
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTVKLKVTKGGPLPFAWDILSPQFTYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGMEASSERMYPEDGALK
                     GEDKLRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(472..491)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     regulatory      901..910
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
       61 ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg atcagttatc
      121 tagatccggt ggatcccggg cccgcggtac cgtcgactgc agaattcgaa gcttgagctc
      181 gagatctgag tccggacttg tacagctcgt ccatgccgcc ggtggagtgg cggccctcgg
      241 cgcgttcgta ctgttccacg atggtgtagt cctcgttgtg ggaggtgatg tccaacttga
      301 tgtcgacgat gtaggcgccg ggcaactgca cgggcttctt ggccttgtag gtggtcttga
      361 cctcggaggt gtagtggccg ccgtccttca gcttcagcct gagcttgtcc tcgcccttca
      421 gggcgccgtc ctcggggtac atccgctcgg aggaggcctc catgcccatg gtcttcttct
      481 gcattacggg gccgtcggag gggaagttgg tgccgcgcag cttcaccttg tagatgaact
      541 cgccgtcctg cagggaggag tcctgagtca cggtcaccac gccgccgtcc tcgaagttca
      601 tcacgcgctc ccacttgaag ccctcgggga aggacagctt gaggtagtcg gggatgtcgg
      661 cggggtgctt cacgtaggcc ttggagccgt aggtgaactg aggggacaag atgtcccagg
      721 cgaagggcag ggggccaccc ttggtcacct tcagcttaac ggtctgaaag ccctcgtagg
      781 ggcggccctc gccctcgccc tcgatctcga actcgtggcc gttcacggag ccctccatgc
      841 gcaccttgaa gcgcatgaac tccttgatga tggccatgtt attctcctcg cccttgctca
      901 ccatggtggc gaccgctagc ggatctga
//
