LOCUS       sequence-47340-e         929 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Shcherbakova DM, Hink MA, Joosen L, Gadella TW, Verkhusha VV
  TITLE     An Orange Fluorescent Protein with a Large Stokes Shift for 
            Single-Excitation Multicolor FCCS and FRET Imaging.
  JOURNAL   J Am Chem Soc. 2012 May 9;134(18):7913-23. doi: 10.1021/ja3018972
  PUBMED    22486524
REFERENCE   2  (bases 1 to 929)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "J Am Chem Soc"; date: "2012-05-9- 9"; volume: "134"; issue: "18"; 
            pages: "7913-23"
COMMENT     Sequence Label: pLSSmOrange-C1
FEATURES             Location/Qualifiers
     source          1..929
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      27..36
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             33..740
                     /codon_start=1
                     /product="monomeric orange fluorescent protein with a large
                     Stokes shift (Shcherbakova et al., 2012)"
                     /label=LSSmOrange
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTVKLKVTKGGPLPFAWDILSPQFTYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGMEASSERMYPEDGALK
                     GEDKLRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     446..465
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    741..806
                     /label=MCS
                     /note="multiple cloning site"
ORIGIN
        1 gtgaaccgtc agatccgcta gcggtcgcca ccatggtgag caagggcgag gagaataaca
       61 tggccatcat caaggagttc atgcgcttca aggtgcgcat ggagggctcc gtgaacggcc
      121 acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcttt cagaccgtta
      181 agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcttg tcccctcagt
      241 tcacctacgg ctccaaggcc tacgtgaagc accccgccga catccccgac tacctcaagc
      301 tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg
      361 tgaccgtgac tcaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc
      421 gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg ggcatggagg
      481 cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgaggac aagctcaggc
      541 tgaagctgaa ggacggcggc cactacacct ccgaggtcaa gaccacctac aaggccaaga
      601 agcccgtgca gttgcccggc gcctacatcg tcgacatcaa gttggacatc acctcccaca
      661 acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg
      721 gcatggacga gctgtacaag tccggactca gatctcgagc tcaagcttcg aattctgcag
      781 tcgacggtac cgcgggcccg ggatccaccg gatctagata actgatcata atcagccata
      841 ccacatttgt agaggtttta cttgctttaa aaacctccca cacctccccc tgaacctgaa
      901 acataaaatg aatgcaattg ttgttgtta
//
