LOCUS       sequence-47347-e         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Lian X, Hsiao C, Wilson G, Zhu K, Hazeltine LB, Azarin SM, Raval KK,
            Zhang J, Kamp TJ, Palecek SP
  TITLE     Robust cardiomyocyte differentiation from human pluripotent stem 
            cells via temporal modulation of canonical Wnt signaling.
  JOURNAL   Proc Natl Acad Sci U S A. 2012 May 29.
  PUBMED    22645348
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2012 May 29."
COMMENT     Sequence Label: pXL003-ishRNA-beta-catenin-2
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    11..29
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             complement(362..406)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    591..824
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
ORIGIN
        1 acttataagt tccctatcag tgatagagaa aaagctggtg gaatgcaagc ttttggatcc
       61 aaaagcttgc attccaccag ctttttttta ctagtgttaa ccgataccgt cgagatccgt
      121 tcactaatcg aatggatctg tctctgtctc tctctccacc ttcttcttct attccttcgg
      181 gcctgtcggg tcccctcggg gttgggaggt gggtctgaaa cgataatggt gaatatccct
      241 gcctaactct attcactata gaaagtacag caaaaactat tcttaaacct accaagcctc
      301 ctactatcat tatgaataat tttatatacc acagccaatt tgttatgtta aaccaattcc
      361 acaaacttgc ccatttatct aattccaata attcttgttc attcttttct tgctggtttt
      421 gcgattcttc aattaaggag tgtattaagc ttgtgtaatt gttaatttct ctgtcccact
      481 ccatccaggt cgtgtgattc caaatctgtt ccagagattt attactccaa ctagcattcc
      541 aaggcacagc agtggtgcaa atgagttttc cagagcaacc ccaaatcccc aggagctgtt
      601 gatcctttag gtatctttcc acagccagga ttcttgcctg gagctgcttg atgccccaga
      661 ctgtgagttg caacagatgc tgttgcgcct caatagccct cagcaaattg ttctgctgct
      721 gcactatacc agacaataat tgtctggcct gtaccgtcag cgtcattgac gctgcgccca
      781 tagtgcttcc tgctgctccc aagaacccaa ggaacaaagc tcctattccc actgctcttt
      841 tttctctctg caccactctt ctctttgcct tggtgggtgc tactcctaat ggttcaattt
      901 ttactacttt atatttatat aattcacttc tccaattgtc cctcatatct cctcctccag
      961 gtctga
//
