LOCUS       sequence-473505-        7722 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7722)
  TITLE     Littman Lab Plasmids
REFERENCE   2  (bases 1 to 7722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: MIGR-mFoxP3
FEATURES             Location/Qualifiers
     source          1..7722
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    520..536
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(544..550,551..568,569..574))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    589..610
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(727..744)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(898..1486)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(978..997)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1657..2448,2449..2517))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2280..2299
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2518..2622)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2690..2708
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2746..2768)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2868..2887
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3081..3103
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3231..3250
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             3450..3966
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    4030..4371
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             4438..4854
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     4784..4806
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     4822..4838
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    6164..6731
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    6181..6733
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6325..6342)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6552..6571
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(6745..6747,6748..6750,6751..7464)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6790..6811)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7051..7070)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7398..7419
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             join(7566..7722,1..358)
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
ORIGIN
        1 tcctgccccg gctcagggcc aagaacagat ggtccccaga tgcggtcccg ccctcagcag
       61 tttctagaga accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc
      121 ttatttgaac taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga
      181 gctcaataaa agagcccaca acccctcact cggcgcgcca gtcctccgat agactgcgtc
      241 gcccgggtac ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc
      301 tgttccttgg gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcatgg
      361 gtaacagttt cttgaagttg gagaacaaca ttctgagggt aggagtcgaa tattaagtaa
      421 tcctgactca attagccact gttttgaatc cacatactcc aatactcctg aaatagttca
      481 ttatggacag cgcagaaaga gctggggaga attgtgaaat tgttatccgc tcacaattcc
      541 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
      601 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
      661 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
      721 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
      781 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
      841 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
      901 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
      961 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     1021 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     1081 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     1141 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     1201 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     1261 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     1321 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     1381 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     1441 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     1501 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     1561 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     1621 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     1681 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     1741 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     1801 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     1861 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     1921 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     1981 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     2041 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     2101 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     2161 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     2221 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     2281 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     2341 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     2401 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     2461 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     2521 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     2581 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     2641 gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat
     2701 cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
     2761 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
     2821 gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca
     2881 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     2941 ataccgcatc aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt
     3001 gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag
     3061 ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcgc aaggaatggt
     3121 gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg
     3181 ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg
     3241 gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg
     3301 gcgtagaggc gattagtcca atttgttaaa gacaggatat cagtggtcca ggctctagtt
     3361 ttgactcaac aatatcacca gctgaagcct atagagtacg agccatagat aaaataaaag
     3421 attttattta gtctccagaa aaagggggga atgaaagacc ccacctgtag gtttggcaag
     3481 ctagcttaag taacgccatt ttgcaaggca tggaaaatac ataactgaga atagagaagt
     3541 tcagatcaag gttaggaaca gagagacagc agaatatggg ccaaacagga tatctgtggt
     3601 aagcagttcc tgccccggct cagggccaag aacagatggt ccccagatgc ggtcccgccc
     3661 tcagcagttt ctagagaacc atcagatgtt tccagggtgc cccaaggacc tgaaatgacc
     3721 ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc ttctgttcgc gcgcttctgc
     3781 tccccgagct caataaaaga gcccacaacc cctcactcgg cgcgccagtc ctccgataga
     3841 ctgcgtcgcc cgggtacccg tattcccaat aaagcctctt gctgtttgca tccgaatcgt
     3901 ggactcgctg atccttggga gggtctcctc agattgattg actgcccacc tcgggggtct
     3961 ttcatttgga ggttccaccg agatttggag acccctgccc agggaccacc gacccccccg
     4021 ccgggaggta agctggccag cggtcgtttc gtgtctgtct ctgtctttgt gcgtgtttgt
     4081 gccggcatct aatgtttgcg cctgcgtctg tactagttag ctaactagct ctgtatctgg
     4141 cggacccgtg gtggaactga cgagttctga acacccggcc gcaaccctgg gagacgtccc
     4201 agggactttg ggggccgttt ttgtggcccg acctgaggaa gggagtcgat gtggaatccg
     4261 accccgtcag gatatgtggt tctggtagga gacgagaacc taaaacagtt cccgcctccg
     4321 tctgaatttt tgctttcggt ttggaaccga agccgcgcgt cttgtctgct gcagcgctgc
     4381 agcatcgttc tgtgttgtct ctgtctgact gtgtttctgt atttgtctga aaattagggc
     4441 cagactgtta ccactccctt aagtttgacc ttaggtcact ggaaagatgt cgagcggatc
     4501 gctcacaacc agtcggtaga tgtcaagaag agacgttggg ttaccttctg ctctgcagaa
     4561 tggccaacct ttaacgtcgg atggccgcga gacggcacct ttaaccgaga cctcatcacc
     4621 caggttaaga tcaaggtctt ttcacctggc ccgcatggac acccagacca ggtcccctac
     4681 atcgtgacct gggaagcctt ggcttttgac ccccctccct gggtcaagcc ctttgtacac
     4741 cctaagcctc cgcctcctct tcctccatcc gccccgtctc tcccccttga acctcctcgt
     4801 tcgaccccgc ctcgatcctc cctttatcca gccctcactc cttctctagg cgccggaatt
     4861 agatctacca tgcccaaccc taggccagcc aagcctatgg ctccttcctt ggcccttggc
     4921 ccatccccag gagtcttgcc aagctggaag actgcaccca agggctcaga acttctaggg
     4981 accaggggct ctgggggacc cttccaaggt cgggacctgc gaagtggggc ccacacctct
     5041 tcttccttga accccctgcc accatcccag ctgcagctgc ctacagtgcc cctagtcatg
     5101 gtggcaccgt ctggggcccg actaggtccc tcaccccacc tacaggccct tctccaggac
     5161 agaccacact tcatgcatca gctctccact gtggatgccc atgcccagac ccctgtgctc
     5221 caagtgcgtc cactggacaa cccagccatg atcagcctcc caccaccttc tgctgccact
     5281 ggggtcttct ccctcaaggc ccggcctggc ctgccacctg ggatcaatgt ggccagtctg
     5341 gaatgggtgt ccagggagcc agctctactc tgcaccttcc cacgctcggg tacacccagg
     5401 aaagacagca accttttggc tgcaccccaa ggatcctacc cactgctggc aaatggagtc
     5461 tgcaagtggc ctggttgtga gaaggtcttc gaggagccag aagagtttct caagcactgc
     5521 caagcagatc atctcctgga tgagaaaggc aaggcccagt gcctcctcca gagagaagtg
     5581 gtgcagtctc tggagcagca gctggagctg gaaaaggaga agctgggagc tatgcaggcc
     5641 cacctggctg ggaagatggc gctggccaag gctccatctg tggcctcaat ggacaagagc
     5701 tcttgctgca tcgtagccac cagtactcag ggcagtgtgc tcccggcctg gtctgctcct
     5761 cgggaggctc cagacggcgg cctgtttgca gtgcggaggc acctctgggg aagccatggc
     5821 aatagttcct tcccagagtt cttccacaac atggactact tcaagtacca caatatgcga
     5881 ccccctttca cctatgccac ccttatccga tgggccatcc tggaagcccc ggagaggcag
     5941 aggacactca atgaaatcta ccattggttt actcgcatgt tcgcctactt cagaaaccac
     6001 cccgccacct ggaagaatgc catccgccac aacctgagcc tgcacaagtg ctttgtgcga
     6061 gtggagagcg agaagggagc agtgtggacc gtagatgaat ttgagtttcg caagaagagg
     6121 agccaacgcc ccaacaagtg ctccaatccc tgcccttgag aattccgccc ccccccccta
     6181 acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt
     6241 ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga
     6301 cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg
     6361 tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt
     6421 gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat
     6481 aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg
     6541 aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg
     6601 taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt
     6661 cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa
     6721 cacgatgata atatggccac aaccatggtg agcaagggcg aggagctgtt caccggggtg
     6781 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtctggc
     6841 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     6901 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
     6961 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     7021 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     7081 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     7141 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     7201 atcatggccg acaagcagaa gaacggcatc aaggcgaact tcaagatccg ccacaacatc
     7261 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     7321 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
     7381 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     7441 ggcatggacg agctgtacaa gtaatgaatt aattaagaat tatcaagctt atcgataccg
     7501 tcgacctgca gccaagctta tcgataaaat aaaagatttt atttagtctc cagaaaaagg
     7561 ggggaatgaa agaccccacc tgtaggtttg gcaagctagc ttaagtaacg ccattttgca
     7621 aggcatggaa aatacataac tgagaataga gaagttcaga tcaaggttag gaacagagag
     7681 acagcagaat atgggccaaa caggatatct gtggtaagca gt
//
