LOCUS       sequence-47357-e       12143 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Non-integrating (episomal) expression of human GLIS1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12143)
  AUTHORS   Okita K, Yamakawa T, Matsumura Y, Sato Y, Amano N, Watanabe A, 
            Goshima N, Yamanaka S
  TITLE     An Efficient Non-viral Method to Generate Integration-Free Human iPS
            Cells from Cord Blood and Peripheral Blood Cells.
  JOURNAL   Stem Cells. 2012 Nov 29. doi: 10.1002/stem.1293.
  PUBMED    23193063
REFERENCE   2  (bases 1 to 12143)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cells.
            2012 Nov 29. doi: 10.1002/stem.1293."
COMMENT     Sequence Label: pCXLE-hGLIS1
FEATURES             Location/Qualifiers
     source          1..12143
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    50..638
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(103..123)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(521..532)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(728..747)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    793..848
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(847..866)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(1209..1225)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1209..1225)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1222..1244)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1233..1249
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1257..1263,1264..1281,1282..1287))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1302..1323
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     protein_bind    1412..1445
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             1906..3831
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     protein_bind    3999..4032
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      4219..6008
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     primer_bind     complement(6550..6567)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6721..7309)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6801..6820)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7480..8271,8272..8340))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8103..8122
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8341..8445)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        8476..8855
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8857..9133
                     /label=chicken beta-actin promoter
     intron          9134..10150
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     10074..10096
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     10158..10177
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    10209..10233
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(join(12133..12143,1..14))
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttgtacaaag tggttcgata attcaagctt cggggactag tcatatgata atcaacctct
       61 ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct
      121 atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat
      181 tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt
      241 caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat
      301 tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc
      361 ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga
      421 caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg cctgtgttgc
      481 cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga
      541 ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc
      601 tcagacgagt cggatctccc tttgggccgc ctccccgcat cggtaaattc actcctcagg
      661 tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa tgccctggct cacaaatacc
      721 actgagatct ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc
      781 tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt
      841 gtctctcact cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt
      901 tggtttagag tttggcaaca tatgcccata tgctggctgc catgaacaaa ggttggctat
      961 aaagaggtca tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag
     1021 ccttgacttg aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat
     1081 ccctaaaatt ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc
     1141 cagtcatagc tgtccctctt ctcttatgga gatccctcga cctgcagccc aagcttggcg
     1201 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     1261 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     1321 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc
     1381 tcaattccga tcatattcaa taacccttaa tataacttcg tataatgtat gctatacgaa
     1441 gttattaggt ctgaagagga gtttacgtcc agccaagctt aggatcaatt ctcatgtttg
     1501 acagcttatc atcgataagc tgatcctcac aggccgcacc cagcttttct tccgttgccc
     1561 cagtagcatc tctgtctggt gaccttgaag aggaagagga ggggtcccga gaatccccat
     1621 ccctaccgtc cagcaaaaag ggggacgagg aatttgaggc ctggcttgag gctcaggacg
     1681 caaatcttga ggatgttcag cgggagtttt ccgggctgcg agtaattggt gatgaggacg
     1741 aggatggttc ggaggatggg gaattttcag acctggatct gtctgacagc gaccatgaag
     1801 gggatgaggg tgggggggct gttggagggg gcaggagtct gcactccctg tattcactga
     1861 gcgtcgtcta ataaagatgt ctattgatct cttttagtgt gaatcatgtc tgacgagggg
     1921 ccaggtacag gacctggaaa tggcctagga gagaagggag acacatctgg accagaaggc
     1981 tccggcggca gtggacctca aagaagaggg ggtgataacc atggacgagg acggggaaga
     2041 ggacgaggac gaggaggcgg aagaccagga gccccgggcg gctcaggatc agggccaaga
     2101 catagagatg gtgtccggag accccaaaaa cgtccaagtt gcattggctg caaagggacc
     2161 cacggtggaa caggagcagg agcaggagcg ggaggggcag gagcaggagg ggcaggagca
     2221 ggaggagggg caggagcagg aggaggggca ggaggggcag gaggggcagg aggggcagga
     2281 gcaggaggag gggcaggagc aggaggaggg gcaggagggg caggaggggc aggagcagga
     2341 ggaggggcag gagcaggagg aggggcagga ggggcaggag caggaggagg ggcaggaggg
     2401 gcaggagggg caggagcagg aggaggggca ggagcaggag gaggggcagg aggggcagga
     2461 gcaggaggag gggcaggagg ggcaggaggg gcaggagcag gaggaggggc aggagcagga
     2521 ggggcaggag gggcaggagg ggcaggagca ggaggggcag gagcaggagg aggggcagga
     2581 ggggcaggag gggcaggagc aggaggggca ggagcaggag gggcaggagc aggaggggca
     2641 ggagcaggag gggcaggagg ggcaggagca ggaggggcag gaggggcagg agcaggaggg
     2701 gcaggagggg caggagcagg aggaggggca ggaggggcag gagcaggagg aggggcagga
     2761 ggggcaggag caggaggggc aggaggggca ggagcaggag gggcaggagg ggcaggagca
     2821 ggaggggcag gaggggcagg agcaggagga ggggcaggag caggaggggc aggagcagga
     2881 ggtggaggcc ggggtcgagg aggcagtgga ggccggggtc gaggaggtag tggaggccgg
     2941 ggtcgaggag gtagtggagg ccgccggggt agaggacgtg aaagagccag ggggggaagt
     3001 cgtgaaagag ccagggggag aggtcgtgga cgtggagaaa agaggcccag gagtcccagt
     3061 agtcagtcat catcatccgg gtctccaccg cgcaggcccc ctccaggtag aaggccattt
     3121 ttccaccctg taggggaagc cgattatttt gaataccacc aagaaggtgg cccagatggt
     3181 gagcctgacg tgcccccggg agcgatagag cagggccccg cagatgaccc aggagaaggc
     3241 ccaagcactg gaccccgggg tcagggtgat ggaggcaggc gcaaaaaagg agggtggttt
     3301 ggaaagcatc gtggtcaagg aggttccaac ccgaaatttg agaacattgc agaaggttta
     3361 agagctctcc tggctaggag tcacgtagaa aggactaccg acgaaggaac ttgggtcgcc
     3421 ggtgtgttcg tatatggagg tagtaagacc tccctttaca acctaaggcg aggaactgcc
     3481 cttgctattc cacaatgtcg tcttacacca ttgagtcgtc tcccctttgg aatggcccct
     3541 ggacccggcc cacaacctgg cccgctaagg gagtccattg tctgttattt catggtcttt
     3601 ttacaaactc atatatttgc tgaggttttg aaggatgcga ttaaggacct tgttatgaca
     3661 aagcccgctc ctacctgcaa tatcagggtg actgtgtgca gctttgacga tggagtagat
     3721 ttgcctccct ggtttccacc tatggtggaa ggggctgccg cggagggtga tgacggagat
     3781 gacggagatg aaggaggtga tggagatgag ggtgaggaag ggcaggagtg atgtaacttg
     3841 ttaggagacg ccctcaatcg tattaaaagc cgtgtattcc cccgcactaa agaataaatc
     3901 cccagtagac atcatgcgtg ctgttggtgt atttctggcc atctgtcttg tcaccatttt
     3961 cgtcctccca acatggggca attgccggaa cccttaatat aacttcgtat aatgtatgct
     4021 atacgaagtt attaggtccc tcgaagaggt tcactagcgg atctcaattg ggcataccca
     4081 tgttgtcacg tcactcagct ccgcgctcaa caccttctcg cgttggaaaa cattagcgac
     4141 atttacctgg tgagcaatca gacatgcgac ggctttagcc tggcctcctt aaattcacct
     4201 aagaatggga gcaaccagca ggaaaaggac aagcagcgaa aattcacgcc cccttgggag
     4261 gtggcggcat atgcaaagga tagcactccc actctactac tgggtatcat atgctgactg
     4321 tatatgcatg aggatagcat atgctacccg gatacagatt aggatagcat atactaccca
     4381 gatatagatt aggatagcat atgctaccca gatatagatt aggatagcct atgctaccca
     4441 gatataaatt aggatagcat atactaccca gatatagatt aggatagcat atgctaccca
     4501 gatatagatt aggatagcct atgctaccca gatatagatt aggatagcat atgctaccca
     4561 gatatagatt aggatagcat atgctatcca gatatttggg tagtatatgc tacccagata
     4621 taaattagga tagcatatac taccctaatc tctattagga tagcatatgc tacccggata
     4681 cagattagga tagcatatac tacccagata tagattagga tagcatatgc tacccagata
     4741 tagattagga tagcctatgc tacccagata taaattagga tagcatatac tacccagata
     4801 tagattagga tagcatatgc tacccagata tagattagga tagcctatgc tacccagata
     4861 tagattagga tagcatatgc tatccagata tttgggtagt atatgctacc catggcaaca
     4921 ttagcccacc gtgctctcag cgacctcgtg aatatgagga ccaacaaccc tgtgcttggc
     4981 gctcaggcgc aagtgtgtgt aatttgtcct ccagatcgca gcaatcgcgc ccctatcttg
     5041 gcccgcccac ctacttatgc aggtattccc cggggtgcca ttagtggttt tgtgggcaag
     5101 tggtttgacc gcagtggtta gcggggttac aatcagccaa gttattacac ccttatttta
     5161 cagtccaaaa ccgcagggcg gcgtgtgggg gctgacgcgt gcccccactc cacaatttca
     5221 aaaaaaagag tggccacttg tctttgttta tgggccccat tggcgtggag ccccgtttaa
     5281 ttttcggggg tgttagagac aaccagtgga gtccgctgct gtcggcgtcc actctctttc
     5341 cccttgttac aaatagagtg taacaacatg gttcacctgt cttggtccct gcctgggaca
     5401 catcttaata accccagtat catattgcac taggattatg tgttgcccat agccataaat
     5461 tcgtgtgaga tggacatcca gtctttacgg cttgtcccca ccccatggat ttctattgtt
     5521 aaagatattc agaatgtttc attcctacac tagtatttat tgcccaaggg gtttgtgagg
     5581 gttatattgg tgtcatagca caatgccacc actgaacccc ccgtccaaat tttattctgg
     5641 gggcgtcacc tgaaaccttg ttttcgagca cctcacatac accttactgt tcacaactca
     5701 gcagttattc tattagctaa acgaaggaga atgaagaagc aggcgaagat tcaggagagt
     5761 tcactgcccg ctccttgatc ttcagccact gcccttgtga ctaaaatggt tcactaccct
     5821 cgtggaatcc tgaccccatg taaataaaac cgtgacagct catggggtgg gagatatcgc
     5881 tgttccttag gaccctttta ctaaccctaa ttcgatagca tatgcttccc gttgggtaac
     5941 atatgctatt gaattagggt tagtctggat agtatatact actacccggg aagcatatgc
     6001 tacccgttta gggttaacaa gggggcctta taaacactat tgctaatgcc ctcttgaggg
     6061 tccgcttatc ggtagctaca caggcccctc tgattgacgt tggtgtagcc tcccgtagtc
     6121 ttcctgggcc cctgggaggt acatgtcccc cagcattggt gtaagagctt cagccaagag
     6181 ttacacataa aggcaatgtt gtgttgcagt ccacagactg caaagtctgc tccaggatga
     6241 aagccactca gtgttggcaa atgtgcacat ccatttataa ggatgtcaac tacagtcaga
     6301 gaaccccttt gtgtttggtc cccccccgtg tcacatgtgg aacagggccc agttggcaag
     6361 ttgtaccaac caactgaagg gattacatgc actgccccgc gaagaagggg cagagatgtc
     6421 gtagtcaggt ttagttcgtc cggggcgggg catcgatcct ctagagtcga cgctagcgga
     6481 tccgctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     6541 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     6601 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     6661 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     6721 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     6781 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     6841 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     6901 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     6961 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     7021 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     7081 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     7141 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     7201 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     7261 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     7321 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     7381 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     7441 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     7501 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     7561 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     7621 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     7681 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     7741 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     7801 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     7861 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     7921 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     7981 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     8041 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     8101 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     8161 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     8221 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     8281 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     8341 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     8401 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     8461 agtgccacct gggtcgacat tgattattga ctagttatta atagtaatca attacggggt
     8521 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
     8581 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
     8641 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
     8701 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
     8761 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
     8821 agtacatcta cgtattagtc atcgctatta ccatggtcga ggtgagcccc acgttctgct
     8881 tcactctccc catctccccc ccctccccac ccccaatttt gtatttattt attttttaat
     8941 tattttgtgc agcgatgggg gcgggggggg ggggggggcg cgcgccaggc ggggcggggc
     9001 ggggcgaggg gcggggcggg cgaggcggag aggtgcggcg gcagccaatc agagcggcgc
     9061 gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag
     9121 cgcgcggcgg gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc
     9181 ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga
     9241 cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggcttg tttcttttct
     9301 gtggctgcgt gaaagccttg aggggctccg ggagggccct ttgtgcgggg ggagcggctc
     9361 ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg
     9421 cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg
     9481 gagcgcggcc gggggcggtg ccccgcggtg cggggggggc tgcgagggga acaaaggctg
     9541 cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca
     9601 accccccctg cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct
     9661 ccgtacgggg cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt
     9721 gccgggcggg gcggggccgc ctcgggccgg ggagggctcg ggggaggggc gcggcggccc
     9781 ccggagcgcc ggcggctgtc gaggcgcggc gagccgcagc cattgccttt tatggtaatc
     9841 gtgcgagagg gcgcagggac ttcctttgtc ccaaatctgt gcggagccga aatctgggag
     9901 gcgccgccgc accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga
     9961 aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc
    10021 tcggggctgt ccgcgggggg acggctgcct tcggggggga cggggcaggg cggggttcgg
    10081 cttctggcgt gtgaccggcg gctctagagc ctctgctaac catgttcatg ccttcttctt
    10141 tttcctacag ctcctgggca acgtgctggt tattgtgctg tctcatcatt ttggcaaaga
    10201 attatcaaac aagtttgtac aaaaaagcag gctccgcggc cgcccccttc accatggcag
    10261 aggcccgcac atccctgtct gcccactgtc ggggcccgct ggccactggc ctgcacccag
    10321 acctggacct cccgggccga agcctcgcca cccctgcgcc ttcctgctac cttctgggca
    10381 gcgaacccag ctctggcctg ggcctccagc ccgagaccca cctccccgag ggcagcctga
    10441 agcggtgctg tgtcttgggc ctacccccca cctccccagc ctcctcctca ccctgtgcct
    10501 cctccgacgt cacctccatc atccgctcct cccagacgtc tctggtcacc tgtgtaaatg
    10561 gactccggag cccccctctg gcgggagatc tggggggccc ttccaagcgg gcccggcctg
    10621 gccctgcatc gacggacagc catgagggca gcttgcaact tgaagcctgc cggaaggcga
    10681 gcttcctgaa gcaggaaccc gcggatgagt tttcagagct ctttgggcct caccagcagg
    10741 gcctgccgcc cccctatccc ctgtctcagt tgccgcctgg cccaagcctt ggaggcctgg
    10801 ggctgggcct gggaggcagg gtggtggccg ggcggcaggc gtgccgctgg gtggactgct
    10861 gtgcagccta tgagcagcag gaggagctgg tgcggcacat cgagaagagc cacaccgacc
    10921 agcgcaaggg cgaggacttc acctgcttct gggctggctg cgtgcgccgc tacaagccct
    10981 tcaacgcccg ctacaagctg ctcatccaca tgcgagtgca ctcgggcgag aagcccaaca
    11041 agtgcatgtt tgaaggctgc agcaaggcct tctcacggct ggagaacctc aagatccacc
    11101 tgaggagcca cacgggcgag aagccgtacc tgtgccagca cccgggttgc cagaaggcct
    11161 tcagcaactc cagcgaccgc gccaagcacc agcgcaccca cctagacacg aagccgtacg
    11221 cctgtcagat ccctggctgc tccaagcgct acacagaccc cagctccctc cgcaagcacg
    11281 tcaaggccca ttcagccaaa gagcagcagg tgcgtaagaa gctgcatgcg ggccctgaca
    11341 ccgaggccga cgtcctgacc gagtgtctgg tcctgcagca gctccacacg tccacacagc
    11401 tggctgccag cgacggcaag ggtggctgtg gcctgggcca ggagctgctc ccaggtgtgt
    11461 atcctggctc catcaccccc cataacggac ttgcatcggg cctcctgccc ccagcgcacg
    11521 acgtaccttc caggcaccac ccgctggatg ccaccaccag ttcccaccac catctgtccc
    11581 ctctgcccat ggctgagagc acccgggatg ggttggggcc cggcctcctc tcaccaatag
    11641 tcagccccct gaaggggctg gggccaccgc cgctgccccc atcctctcag agccattctc
    11701 cggggggcca gcccttcccc acactcccca gcaagccgtc ctacccaccc ttccagagcc
    11761 ctccaccccc gcctctgccc agcccacaag gttaccaggg cagtttccac tccatccaga
    11821 gttgcttccc ctatggcgac tgctaccgga tggctgaacc agcagccggt ggggacggac
    11881 tggtcgggga gacccacggt ttcaaccccc tgcggcccaa tggctaccac agcctcagca
    11941 cgcccttgcc tgccacaggc tatgaggccc tggctgaggc ctcatgcccc acagcgctgc
    12001 cacagcagcc atctgaagat gtggtgtcca gcggccccga ggactgtggc ttcttcccca
    12061 atggagcctt tgaccactgc ctgggccaca tcccctccat ctacacagac acctgaaagg
    12121 gtgggcgcgc cgacccagct ttc
//
