LOCUS       sequence-473626-        9350 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  transient overexpression of EMSY in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9350)
  AUTHORS   Marzio A, Kurz E, Sahni JM, Di Feo G, Puccini J, Jiang S, Hirsch CA,
            Arbini AA, Wu WL, Pass HI, Bar-Sagi D, Papagiannakopoulos T, Pagano 
            M
  TITLE     EMSY inhibits homologous recombination repair and the interferon 
            response, promoting lung cancer immune evasion.
  JOURNAL   Cell. 2022 Jan 6;185(1):169-183.e19. doi: 
            10.1016/j.cell.2021.12.005. Epub 2021 Dec 27.
  PUBMED    34963055
REFERENCE   2  (bases 1 to 9350)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2021.12.005"; journalName: "Cell"; date: "2022-01-6-
            6"; volume: "185"; issue: "1"; pages: "169-183.e19"
COMMENT     Sequence Label: pcDNA3.1 2xFLAG-2xSTREP-Emsy (mouse)
FEATURES             Location/Qualifiers
     source          1..9350
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     386..405
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        480..584
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(585..653,654..1445)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(803..822)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1616..2204
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2105..2124
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2358..2375
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2492..2513
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2528..2533,2534..2551,2552..2558)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2566..2582
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2571..2593
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2590..2606
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2590..2606
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    2655..2776
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2666..2685)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2720..2739
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(2950..3744)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3061..3080)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     3671..3690
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(3811..4140)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3825..3960
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3879..3898)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     4125..4145
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(4154..4582)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4264..4285)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4476..4495
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    4628..4852
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     4841..4858
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(8358..8369)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(8715..8738)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             complement(8775..8798)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             complement(8814..8837)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(8853..8876)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(8917..8936)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8918..8936)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(8981..9184)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(9010..9030)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        join(9185..9350,1..214)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 cgtaaatact ccacccattg acgtcaatgg aaagtcccta ttggcgttac tatgggaaca
       61 tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg cgggccattt
      121 accgtaagtt atgtaacgcg gaactccata tatgggctat gaactaatga ccccgtaatt
      181 gattactatt aataactagt caataatcaa tgtcaacgcg tatatctggc ccgtacatcg
      241 cgaagcagcg caaaacgcct aaccctaagc agattcttca tgcaattgtc ggtcaagcct
      301 tgccttgttg tagcttaaat tttgctcgcg cactactcag cgacctccaa cacacaagca
      361 gggagcagat actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
      421 aggggatcgg gagatctccc gatccgtcga cgtcaggtgg cacttttcgg ggaaatgtgc
      481 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
      541 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
      601 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
      661 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
      721 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
      781 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
      841 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
      901 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
      961 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     1021 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     1081 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     1141 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     1201 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     1261 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     1321 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     1381 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     1441 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     1501 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     1561 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     1621 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     1681 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     1741 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     1801 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     1861 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     1921 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     1981 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     2041 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     2101 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     2161 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     2221 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     2281 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     2341 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     2401 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     2461 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     2521 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     2581 aatttcacac aggaaacagc tatgaccatg attacgccaa gctctagcta gaggtcgacg
     2641 gtatacagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
     2701 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     2761 ctgcaataaa caagttgggg tgggcgaaga actccagcat gagatccccg cgctggagga
     2821 tcatccagcc ggcgtcccgg aaaacgattc cgaagcccaa cctttcatag aaggcggcgg
     2881 tggaatcgaa atctcgtgat ggcaggttgg gcgtcgcttg gtcggtcatt tcgaacccca
     2941 gagtcccgct cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg
     3001 agcggcgata ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc
     3061 aatatcacgg gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca
     3121 gtcgatgaat ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc
     3181 atgggtcacg acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc
     3241 ggctggcgcg agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc
     3301 catccgagta cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc
     3361 cggatcaagc gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg
     3421 agcaaggtga gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct
     3481 tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca
     3541 cgatagccgc gctgcctcgt cctgcagttc attcagggca ccggacaggt cggtcttgac
     3601 aaaaagaacc gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat
     3661 tgtctgttgt gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc
     3721 gtgcaatcca tcttgttcaa tcatgcgaaa cgatcctcat cctgtctctt gatcagatcc
     3781 gaaaatggat atacaagctc ccgggagctt tttgcaaaag cctaggcctc caaaaaagcc
     3841 tcctcactac ttctggaata gctcagaggc agaggcggcc tcggcctctg cataaataaa
     3901 aaaaattagt cagccatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggat
     3961 gggcggagtt aggggcggga ctatggttgc tgactaattg agatgcatgc tttgcatact
     4021 tctgcctgct ggggagcctg gggactttcc acacctggtt gctgactaat tgagatgcat
     4081 gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacccta actgacacac
     4141 attccacaga attaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag
     4201 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt
     4261 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga
     4321 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
     4381 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct
     4441 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc
     4501 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt
     4561 aatgcgccgc tacagggcgc gtggggatac cccctagagc cccagctggt tctttccgcc
     4621 tcagaagcca tagagcccac cgcatcccca gcatgcctgc tattgtcttc ccaatcctcc
     4681 cccttgctgt cctgccccac cccacccccc agaatagaat gacacctact cagacaatgc
     4741 gatgcaattt cctcatttta ttaggaaagg acagtgggag tggcaccttc cagggtcaag
     4801 gaaggcacgg gggaggggca aacaacagat ggctggcaac tagaaggcac agtcgaggct
     4861 gatcagcggg tttaaacggg ccctctagac tcgagctagg accgttctac agcgctctga
     4921 gaggggctgg gctcagtccc gtcctccgtg ctgctgtcta tgtcctgctc catggctgtc
     4981 tcgtcgtcat ctagctgctg actggtgaag ttgttcaact ctagaagccc actgggctcc
     5041 accaccacat tggagctgga gtgacaagga atagcatact ggggaatggc ctggatgata
     5101 atttctgttg ggctctcttc agctttcttt tggcctacat tctgaatacg catgaactgg
     5161 cctgtcgtag gcactcctgc tgcttcgatt tttctggtcg ttaggggagg gccaacagca
     5221 gatggacttg aacaggactc tctacatttc gtcatccctg ccaccaccac atgggtggag
     5281 ggtagagctc ctgcctcacc agtgagcaca ttggtggcta gagccttctt tgggggatcc
     5341 actaccatat gttctacatt tgtatcaatc tgagcttcca tcaaagacat ttgcaaaatg
     5401 tctgacactg ctgtcttctc agaggcctga atgggagata tgcttgtaac tggtgatgtc
     5461 agcttaggaa cagaactgcc accagttatc tttgtaactg tgggaggctg gcgcccctca
     5521 aatgttatct ttgtaacaga ggatccttga gttataattg gctgctccac ctggcttgca
     5581 ggctgtttct ctgaagaggg gagtgccatc tggctctgag gagtttgggg ttgcacgtag
     5641 atttttggtt ggttcggagc ttgctgcagt ttaggaagct gctgtgtggt tttcacggca
     5701 agcacctgta ctacagtttg cgggggctgt gccattaagg taggaagctg cctgtctttg
     5761 gccaccacag tatgctgtgt gtgctgtgca tctggcttgg gctgtgcttg ttcctgctgg
     5821 agaggattca gtttctgatg cctgatagag agctggggca tctgtgtgag tttgtgctgg
     5881 agttggtctg cctgcaggtg gatggtctgc ttctgtttgg tctggaagca ctgcagagtt
     5941 ttcacttgca gctgagtctg ttccagctgg ggctggctaa gtttctgctg tttggttgac
     6001 tgcgattgtg tcagggcaga gcggtaaaac agacctgtct gaggatccaa tgtgtccatc
     6061 tcttcaacct caccctcctc agttccaagt tcctcactgt gtttcgtaaa agcggtgtga
     6121 ctgcttaatg cctgctgcat gatgtgggtg attgctccag gggaagacgc tgggatggac
     6181 tttactacca ccgaagtctg cgtcgcagtc tgtgactgag ccacatgttg aagcagggtc
     6241 cgctggggct gggaaggctg ttggggctgg gagcgatggc tgaccgtggt gatgtattca
     6301 gcaactagtt ctgactctac cacagcaatg gatggactct caggagaatg tctcttttcc
     6361 tgggtcatct gaataggtac tgccatttga ctcaagtcaa tagtgggttg tcttggttta
     6421 gattccaatt tttcctttac tgttgtctgt tggagttcta acagagcttt gattgttgaa
     6481 gtggctgatt gtgattcact ggatacatcc tgatatatta tggtggggct agtctccatt
     6541 gcaatctcat gttctgacca atcctgacgc cgggaagcaa ttacatgaac tacaggctgg
     6601 gaatcttgag aactctggga ggactctgtg gaagaagaac tactgtctgt gtaaccctgt
     6661 ggctcctctt ttccttcctg agcagatgaa ttactggcat ctgctaccct ggatgcttgc
     6721 tgtaatgttt ctgttaccag ctgccttgtt tgttcactga caactgtagc ttgaaatgtc
     6781 actggtttgg gtttaattag aacctgtgtt ttcccttgtt gtccatacac aatttttgtt
     6841 ggaatgattt tagctgctgg ctgtactgcg gaacctattc ctttgggctg tgttacaatc
     6901 attttggtga tgggtcttgt agcagtaatt atagctggct tggctccagc tggcactgtc
     6961 tgaagagtct tttctcctcc agcatttagc aaggttgtga caacgttttt gccagggagg
     7021 ccttgaatgg tcgttccttt tcctgtagtc ttttgtacaa caatcacatt gggcttggaa
     7081 gtcattggga ttgtagtgat tttggtagtc gttccagaaa caatattact gctaattatc
     7141 ttgcctccca aggtagctaa tgatttgggt actacagtga tgatgctgcc actggtagtt
     7201 ttcacatagg ttgcagctcc aggggttgta gccacacgac ctaaggatgg gctcactgtt
     7261 ggccgggtat aggttgcctg agtaccggta ggagtggtta ccagtttagt tgtcataatt
     7321 gcaccattac tgctaaccac cataattggg gaattgctac tggtgggtag agttgcagtc
     7381 actggtttgg gtaagatttt acttggttga acctgctgtg tgatgatttt aacacctgac
     7441 tcctgtttga tctggatggt aggtttgata ccaggcggca atgctgactg ctgaggctgc
     7501 tgctgctgag acactgaaga aggctgggcc actggctgct gtgtctgttg ttgtacttgc
     7561 tgtaactgct gcttaggaga ctgctgatgc tgtttaggaa actgtgcaag aatctgggta
     7621 gggccactca ctaagctttt gggggaagga agtcttgtcg atgccacttt gattgctgat
     7681 gtggatacac cttgtgctac tgttgacatg accacggacg gtgtggaaga taccaccgtt
     7741 gttactgcaa ctgtactaga aataggtgat gacgtagaac tgctgttgcc attgctgcta
     7801 ctggtgacca gagaggcctg ggacgcagtt ataacaacag gtggcttctg agtcgtggag
     7861 gcaatgactg atgttggtac aagcttagtg actgctgcat agttatgaga tttggagaga
     7921 atgttgggca caaaggttga acttggtgat gtggtcacta taataacctt ctgtgttgtt
     7981 gtgtttgttg tctgtgttga tggtttggta aacgttattt tcacaggtga catatggggt
     8041 ggtaaggagt tggcaatgct ctgcataatg ttgctcatct tgggacttcc actcacaggc
     8101 acagtgatcg tctttgagac cggaacaaca gcctttggaa cttccttgag gacaacaggg
     8161 gaggagctag aggagtttgt tcgcctgcgt tttctgggtt tttcatcttc atctgagcag
     8221 ctaacgccgt ctttgcttgc tgtctctgca ggcacaggaa gggacgcatt gtgctggaca
     8281 gctgcattgg ccacagcatt cgctgttaca gtgaaggcag tctggggaac cagcctgggc
     8341 atcagaggta ccaatctacg tccttcgatg gaccactctg aagaactatt aggtccagac
     8401 atactgtaac ttggtctttc acccaaatat aaggataaat tcattttatg tgcaattgtt
     8461 gttaaccgtt catcgtttac tgctctccga acttcagcac ggtggcgctc tgttgagatg
     8521 ctaaggactt ttgagagctc tccaagaaga tctttctttt ccttggtgag gtccccttgt
     8581 gcccgaagtg cactgataac tccagcatat gcctccagtt ccaattttcg aagaatccgt
     8641 ttgcattcat ccctgctgag atccaaaagg gttggccaca caacagggaa ttccaccaca
     8701 ctggatccag ctcctttctc gaactgcggg tggctccagc ttcctcctcc gctgcctccg
     8761 ccgcttcctc ctcccttctc gaactgaggg tggctccagc tggcggccga ccctttatca
     8821 tcatcatctt tataatccat agatcctggt accttgtcat cgtcgtcctt gtagtccatg
     8881 gtaagcttaa gtttaaacgc tagccagctt gggtctccct atagtgagtc gtattaattt
     8941 cgataagcca gtaagcagtg ggttctctag ttagccagag agctctgctt atatagacct
     9001 cccaccgtac acgcctaccg cccatttgcg tcaatggggc ggagttgtta cgacattttg
     9061 gaaagtcccg ttgattttgg tgccaaaaca aactcccatt gacgtcaatg gggtggagac
     9121 ttggaaatcc ccgtgagtca aaccgctatc cacgcccatt gatgtactgc caaaaccgca
     9181 tcaccatggt aatagcgatg actaatacgt agatgtactg ccaagtagga aagtcccata
     9241 aggtcatgta ctgggcataa tgccaggcgg gccatttacc gtcattgacg tcaatagggg
     9301 gcgtacttgg catatgatac acttgatgta ctgccaagtg ggcagtttac
//
