LOCUS       sequence-473873-        1038 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses enoyl-ACP reductase FabI [Wolbachia endosymbiont of Brugia
            malayi] under T7 with HIS tag in bacterial cells).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1038)
  TITLE     Josh Beckham Lab plasmids 2025
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: WoFabI-pNIC-Bsa4
FEATURES             Location/Qualifiers
     source          1..1038
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             28..50
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             61..78
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             103..123
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ggggggggaa attccctcta gaataatttt gtttaacttt aagaaggaga tatacatatg
       61 caccatcatc atcatcattc ttctggtgta gatctgggta ccgagaacct gtacttccaa
      121 tccatggcga tttctctgct gcagggcaaa aaaggtctga tcaccggcat tatcaacaaa
      181 cgttctatcg cgtacggtat cgttaaaacc ctgagcgaac atggtgcaga atctgcggtt
      241 acctaccaga acgaaatcgt caaagaacgt ctgctgagca ttgccgcgga actgaacgtt
      301 gaactggttc tgaactgcga cgttgcgaac gaaggtacca tcgacgacgt tttcaaatct
      361 atcgaagaaa aatggggtac gctcgacttt ctggttcacg caatcgcatt ctctgacaaa
      421 aacgaactgt ctggtaaata cgttaacacc tctctgaaca atttccaaaa cgccatgaac
      481 atctcttgct actctttcac tgcgctggcg caacgcgctg aaaagatgat gccaaatggt
      541 ggttctctcc tgacgctgtc ttactacggt gcggaaaaag ttatgccgaa ctacaatgtt
      601 atgggtctgt gcaaagcggc tctggaagcg tctgttaaat acctggcgtg cgacctgggt
      661 ccgcagaaca tccgtgtaaa cgcgatctct gcgggtccga tccgtaccct ggcgagctct
      721 ggtatttctg acttccactt catcagcgaa tggaaccgta acaactctcc gctgcgtcgt
      781 aacaccaccc tggaagacgt tggtaaagcc gccctgtacc tgctgtctga cctgtcctct
      841 ggcacgacgg gtgagatcct gcacgttgac tctggttaca acgtagttgg tatgaaagcg
      901 atcgattcta acatcattaa ttcttaccaa aattgacagt aaaggtggat acggatccga
      961 attctagctc cgtcgacaag cttgcggccg cantcgagca ctacaccacc tcccttgaaa
     1021 tccgttggct aacaaagc
//
