LOCUS       sequence-473963-       11106 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Used to express any CDS fused to 3xHA-TurboID (N-terminal) in T. 
            cruzi. Tetracycline regulated. Modified from Alonso et al. (2014). .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11106)
  TITLE     Serra Lab Plasmids
REFERENCE   2  (bases 1 to 11106)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pTcTurboID-GW
FEATURES             Location/Qualifiers
     source          1..11106
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(87..134)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        162..180
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    187..205
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     CDS             464..490
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             494..520
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             524..550
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             557..1513
                     /codon_start=1
                     /product="mutant of the E. coli biotin ligase BioID 
                     engineered for improved catalytic efficiency (Branon et 
                     al., 2018)"
                     /label=TurboID
                     /translation="KDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLRDW
                     GVDVFTVPGKGYSLPEPIPLLNAKQILGQLDGGSVAVLPVVDSTNQYLLDRIGELKSGD
                     ACIAEYQQAGRGSRGRKWFSPFGANLYLSMFWRLKRGPAAIGLGPVIGIVMAEALRKLG
                     ADKVRVKWPNDLYLQDRKLAGILVELAGITGDAAQIVIGAGINVAMRRVEESVVNQGWI
                     TLQEAGINLDRNTLAATLIRELRAALELFEQEGLAPYLPRWEKLDNFINRPVKLIIGDK
                     EIFGISRGIDKQGALLLEQDGVIKPWMGGEISLRSAEK"
     protein_bind    1559..1683
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(1708..1713,1714..1731,1732..1738)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             1792..2451
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1858..1877)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2793..3098
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2977..2996
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(3139..3263)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      3795..3842
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      3927..3974
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(4021..4037)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4021..4037)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4034..4056)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4045..4061
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4069..4075,4076..4093,4094..4099))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4114..4135
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4252..4269)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4423..5011)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4503..4522)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5182..5973,5974..6042))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6043..6147)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6215..6233
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6271..6293)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6393..6412
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6606..6628
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     6620..6637
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     6621..6637
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(9513..10538)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             complement(9513..10538)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(11010..11028)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(join(11061..11106,1..2))
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 agttattgct cagcggtggc agcagccaac tcagcttcct ttcgggcttt gttagcagcc
       61 ctatccggat atagttctcc tttcagcaaa aaacccctca agacccgttt agaggcccca
      121 aggggttatg ctagttattg ctcagcgggc tagcacctga ttaatacgac tcactatagg
      181 gagatctccc tatcagtgat agagatctag cccctcgaga tcccccaacg agtttcttca
      241 aaatatgcag cggattcact aagaaacatt ttcacgcacg aaagcgaaat tattatgatt
      301 gttattataa tactttttct ttgttgtttt atccacttat tattgttgtg ttaaattgtt
      361 tttacctttt ttcttttcca acttctttta tgatgtcttt tctttttttt tttggctcta
      421 taagttgtct tgcggccgcc ggcatcgatt ggccgccacc atgtacccgt atgatgttcc
      481 ggattacgct ggctatccct acgacgtgcc cgactatgcc gggtacccct atgacgtccc
      541 agactacgca gctagcaaag acaatactgt gcctctgaag ctgatcgctc tcctggctaa
      601 tggcgagttc catagtggcg aacagctggg agaaaccctg ggcatgtcca gggccgctat
      661 caacaagcac attcagactc tgcgcgactg gggcgtggac gtgttcaccg tgcccggaaa
      721 gggctactct ctgcccgagc ctatcccgct gctgaacgct aaacagattc tgggacagct
      781 ggacggcggg agcgtggcag tcctgcctgt ggtcgactcc accaatcagt acctgctgga
      841 tcgaatcggc gagctgaaga gtggggatgc ttgcattgca gaatatcagc aggcagggag
      901 aggaagcaga gggaggaaat ggttctctcc ttttggagct aacctgtacc tgagtatgtt
      961 ttggcgcctg aagcggggac cagcagcaat cggcctgggc ccggtcatcg gaattgtcat
     1021 ggcagaagcg ctgcgaaagc tgggagcaga caaggtgcga gtcaaatggc ccaatgacct
     1081 gtatctgcag gatagaaagc tggcaggcat cctggtggag ctggccggaa taacaggcga
     1141 tgctgcacag atcgtcattg gcgccgggat taacgtggct atgaggcgcg tggaggaaag
     1201 cgtggtcaat cagggctgga tcacactgca ggaagcaggg attaacctgg acaggaatac
     1261 tctggccgct acgctgatcc gagagctgcg ggcagccctg gaactgttcg agcaggaagg
     1321 cctggctcca tatctgccac ggtgggagaa gctggataac ttcatcaata gacccgtgaa
     1381 gctgatcatt ggggacaaag agattttcgg gattagccgg gggattgata aacagggagc
     1441 cctgctgctg gaacaggacg gagttatcaa accctggatg ggcggagaaa tcagtctgcg
     1501 gtctgccgaa aaggaattca gcagggccga cgaatcgata cgcgtggatc cgtcgatcac
     1561 aagtttgtac aaaaaagctg aacgagaaac gtaaaatgat ataaatatca atatattaaa
     1621 ttagattttg cataaaaaac agactacata atactgtaaa acacaacata tccagtcact
     1681 atggcggccg cattaggcac cccaggcttt acactttatg cttccggctc gtataatgtg
     1741 tggattttga gttaggatcc gtcgagattt tcaggagcta aggaagctaa aatggagaaa
     1801 aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga acattttgag
     1861 gcatttcagt cagttgctca atgtacctat aaccagaccg ttcagctgga tattacggcc
     1921 tttttaaaga ccgtaaagaa aaataagcac aagttttatc cggcctttat tcacattctt
     1981 gcccgcctga tgaatgctca tccggaattc cgtatggcaa tgaaagacgg tgagctggtg
     2041 atatgggata gtgttcaccc ttgttacacc gttttccatg agcaaactga aacgttttca
     2101 tcgctctgga gtgaatacca cgacgatttc cggcagtttc tacacatata ttcgcaagat
     2161 gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag ggtttattga gaatatgttt
     2221 ttcgtctcag ccaatccctg ggtgagtttc accagttttg atttaaacgt ggccaatatg
     2281 gacaacttct tcgcccccgt tttcaccatg ggcaaatatt atacgcaagg cgacaaggtg
     2341 ctgatgccgc tggcgattca ggttcatcat gccgtttgtg atggcttcca tgtcggcaga
     2401 atgcttaatg aattacaaca gtactgcgat gagtggcagg gcggggcgta aacgcgtgga
     2461 tccggcttac taaaagccag ataacagtat gcgtatttgc gcgctgattt ttgcggtata
     2521 agaatatata ctgatatgta tacccgaagt atgtcaaaaa gaggtatgct atgaagcagc
     2581 gtattacagt gacagttgac agcgacagct atcagttgct caaggcatat atgatgtcaa
     2641 tatctccggt ctggtaagca caaccatgca gaatgaagcc cgtcgtctgc gtgccgaacg
     2701 ctggaaagcg gaaaatcagg aagggatggc tgaggtcgcc cggtttattg aaatgaacgg
     2761 ctcttttgct gacgagaaca ggggctggtg aaatgcagtt taaggtttac acctataaaa
     2821 gagagagccg ttatcgtctg tttgtggatg tacagagtga tattattgac acgcccgggc
     2881 gacggatggt gatccccctg gccagtgcac gtctgctgtc agataaagtc tcccgtgaac
     2941 tttacccggt ggtgcatatc ggggatgaaa gctggcgcat gatgaccacc gatatggcca
     3001 gtgtgccggt ctccgttatc ggggaagaag tggctgatct cagccaccgc gaaaatgaca
     3061 tcaaaaacgc cattaacctg atgttctggg gaatataaat gtcaggctcc cttatacaca
     3121 gccagtctgc aggtcgacca tagtgactgg atatgttgtg ttttacagta ttatgtagtc
     3181 tgttttttat gcaaaatcta atttaatata ttgatattta tatcatttta cgtttctcgt
     3241 tcagctttct tgtacaaagt ggtgatcgag gcaggcccaa gcattgtgca caacaaatgc
     3301 ttttagtaat ctctgcataa aggtgaatag ttttcctatc accggaatta tttttcttcc
     3361 gtgggcttac cgttttctgt ccttgtaagg tttctgagat cgcgttccca tatatatata
     3421 tctttttttc aagtgttgat ttactgaagg agcttttaat aataatttca agccagtcat
     3481 aatcggaatc tgaacattca agagattttt tttttcaaac cttcctccat gtttgcgtga
     3541 gcgagctagt cgataggagt ttgcagcttt gagctttgat ggcttgttgg tggtcgttga
     3601 cttttttttt gtgtgtgatg ctcaccctca cggcggtttg atctttgtcc atttcttgtt
     3661 ttttcttctt cttttttcac ataacaaaca aaccagggag aaaaactttt ttaggttttt
     3721 tgtttatatc gcgttttttt ttgtttctaa ggatgtctga cgatcgaatc aggtgctagc
     3781 ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt
     3841 tgctgaaagg agaactatat ccggataggg ctgctaacaa agcccgaaag gaagctgagt
     3901 tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct
     3961 tgaggggttt tttgctgaaa ggaggaacta tatccgggga tcaagcttgg cgtaatcatg
     4021 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     4081 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     4141 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     4201 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     4261 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     4321 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     4381 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     4441 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     4501 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     4561 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     4621 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     4681 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     4741 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     4801 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     4861 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     4921 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     4981 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     5041 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     5101 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     5161 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     5221 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     5281 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     5341 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     5401 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     5461 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     5521 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     5581 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     5641 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     5701 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     5761 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     5821 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     5881 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     5941 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     6001 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     6061 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     6121 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     6181 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     6241 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     6301 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     6361 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
     6421 gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
     6481 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     6541 attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     6601 gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttcgagctca tttgtgtcta
     6661 gtacatcatg gagcggtatt ctctccggtg gcccaggtgg cgtcggaagg aattggtgtc
     6721 cctcaggatg tttggttttt cgtttttttt tttgcggtct tttgtttcgc atcgccgtat
     6781 cgtgtgcggc gcgaccttct ttttggctca ttggtcagtg aggtttataa taaagtggat
     6841 cacttgggtt tttggccctt tgcggcgcac ccgcggaatg ggggagggat tttcataatg
     6901 tagtgtacat tgtgtgtggg tcgcaaaatg tcatgccgtg tgcaatgttt atttgcgtgc
     6961 tgtgcgtgtc cttttgtgat tcccaccact tgtgtgtatt ttaccttatc gtctctccct
     7021 gccgttggct gtgcgtgaat tgtgcttcgc attcgtggga aatcaaaagg ggaatcaacg
     7081 tgttcttttt tttcagaagg ggagtcaaag cgccgtgtgg atgccaagtc tgtgcaaaag
     7141 ttaactatta ttactactgc tgctgctgct gccactccta catatgcgca caagtacact
     7201 atatatatgc gaatacacct gtgtgtatta tgctatgtgt gtgtctgtgt gggtgtgtag
     7261 gtgatggggt agtggttgta ttatagtata gtatatatag tagtatacgg caaaaggcaa
     7321 agacacaagc acggcgagta cgacgaacaa acccatttca cacatgagtg tgcgccacac
     7381 acgcgactgc tccttttttt tctgccttgc caccacccca tatgtatatt atatatatat
     7441 atatataagg gtgtgtgtgt ggtgagagaa aataggaaat gattgcgtgt gtgtcgccgc
     7501 cacgaacctg tcagatgctt tcttacggta ttttgttgca tgcacccgcg tacacacaat
     7561 atacacacac aaaaaaaaca ccgatatgat ggcgagtgtg cgattctcct tttattgtct
     7621 ttttcctcct tttctgtgct ctctgctgtg tgccaataaa acacactcct cctatgtttg
     7681 gtgtagtgtg tgttatttat tgtccgcgca aaaaaaaaag cgagtagaga aacgggggaa
     7741 aaagtagtaa ataaatggag gactgcttcg cagctgccct gccgcactgt ttttgctgct
     7801 gggttcgcaa cgcgatgctg ttgagagtga aatcacatca caaaaagcac ccctcagact
     7861 ggtgtgaagt gtgaagtatg tgtacaaatg cgtgtgtgtg tgtatacaca ttgacgaata
     7921 gcataccagg gaaaaaaggg aggacactct ctttcgtgcg tgctgaactc tgtgtgtgtg
     7981 tgctgccgtt ttgtttgcag cttgaaatgc ttggttgaat agaaatcggt acttgattgt
     8041 tgccgcattt cgaggaattt catccaaaaa aaaaaagagg tcaacaacaa ccaactgaca
     8101 aactttgtgt tggacctgtg tatgtggtgg tgtggtgggg gaagagaaac acacacacag
     8161 acaaacacaa acaccatgtc attttggcat gcgtgtgcgt gcgtgcgtgc gttgaatatc
     8221 ctccacacac aaatatatat atataataat accacaacaa catttcaaag gatcatcaat
     8281 gtgtgtgaga cgtgtgcgtt tgtagcgtgt gtgtatatgt gttttgtgtg ttgtgtggtg
     8341 taatgtgtgt gtgtgtcgtt cgtgcatata caccttatac acacacacat ataatacata
     8401 cataaataca cacacacggc gaacgccgcg gaccaacaac ttgagatctg gttgattctg
     8461 ccagtagtca tatgcttgtt tcaaggactt agccatgcac gcctcagact agtagctcgg
     8521 tgcaccctgg gatatttgcg cacccacctt tcccgtcgca atcggcgccg tccgaacgcg
     8581 gaaatattcc gagaataata cggctgggct gcgccgcgcg ttccccgcgt gaaacaatta
     8641 ttgtgcgatg tgcagcatcc tattgtggtg ctttaccgca caccagtttc cttgcggggc
     8701 ttatttggtg tgtcggattt ttaatttttt gatgattcgg aggaattttt tacaattttt
     8761 gggtacccct aaaaaacttt ttggagtttc cggggttcga tccaacttgt gcttttttaa
     8821 ccgtccattt gtgtgagctc caccgcggtg gcggccgatc ccccgggctg caggaattcg
     8881 atccacacgg ctagcatact ctagcggcat atacacaggt gggctataat gcccagtatt
     8941 gttcgaaaaa gtccaaagaa caacaacact acgacgcata atgcacaaac actaaactac
     9001 accttaaaca ccatcagtga agaaaaagaa aaaaaaagaa aaacagctta aatccttaaa
     9061 aaaaaaaaat cacactaaat taaaacagtc agttataaac aaaaagaaac tgaacaacca
     9121 aaatcttcca ataacgcaaa gttgtaaaaa attaccacaa gctgcatcat acacccaatc
     9181 aaatcatcta cattttcctt gcaatacccc aaatcctcgt acattgatta aacattattt
     9241 cactcatcct tccgcatcat taaattgctg ctttttgttt cccaaagatg accataaata
     9301 taatttcaaa aaaaaaaaag aaaaaaaagt tatatatttt tttgttgttg tccttaaagg
     9361 gaaaagtaaa attgttttgc tcttgacact gtgtcacttg gatcgtggca gcaccggtaa
     9421 cgatcatcac gggacacgca tcgtgtatta taagatccct attcgcaaaa gaaagacctg
     9481 aagtcatcgc cacgcctaca acggcatcta ctctattcct ttgccctcgg acgagtgctg
     9541 gggcgtcggt ttccactatc ggcgagtact tctacacagc catcggtcca gacggccgcg
     9601 cttctgcggg cgatttgtgt acgcccgaca gtcccggctc cggatcggac gattgcgtcg
     9661 catcgaccct gcgcccaagc tgcatcatcg aaattgccgt caaccaagct ctgatagagt
     9721 tggtcaagac caatgcggag catatacgcc cggagccgcg gcgatcctgc aagctccgga
     9781 tgcctccgct cgaagtagcg cgtctgctgc tccatacaag ccaaccacgg cctccagaag
     9841 aagatgttgg cgacctcgta ttgggaatcc ccgaacatcg cctcgctcca gtcaatgacc
     9901 gctgttatgc ggccattgtc cgtcaggaca ttgttggagc cgaaatccgc gtgcacgagg
     9961 tgccggactt cggggcagtc ctcggcccaa agcatcagct catcgagagc ctgcgcgacg
    10021 gacgcactga cggtgtcgtc catcacagtt tgccagtgat acacatgggg atcagcaatc
    10081 gcgcatatga aatcacgcca tgtagtgtat tgaccgattc cttgcggtcc gaatgggccg
    10141 aacccgctcg tctggctaag atcggccgca gcgatcgcat ccatggcctc cgcgaccggc
    10201 tgcagaacag cgggcagttc ggtttcaggc aggtcttgca acgtgacacc ctgtgcacgg
    10261 cgggagatgc aataggtcag gctctcgcta aattccccaa tgtcaagcac ttccggaatc
    10321 gggagcgcgg ccgatgcaaa gtgccgataa acataacgat ctttgtagaa accatcggcg
    10381 cagctattta cccgcaggac atatccacgc cctcctacat cgaagctgaa agcacgagat
    10441 tcttcgccct ccgagagctg catcaggtcg gagacgctgt cgaacttttc gatcagaaac
    10501 ttctcgacag acgtcgcggt gagttcaggc tttttcatat ctcattgccc cccgggatca
    10561 gatctgcagg gcttcccaac cttaccagag ggcgccccag ctggcaagct agttctagaa
    10621 ttctgttcaa tgtaattgtt gttggtagat ggctttcaaa gtaaaaatat cactttctgc
    10681 attcgtgcag gaaacgtggg aaaagagaga caagtggaat gaaggggagg ttgacaattg
    10741 tgggttttgt gtttcttttc ttgcattcat ttacggccgc tgtaattatt attattatta
    10801 ttattatttg tagaggacag acataaaata aatttgaggt attttccgca ttttgttttt
    10861 gtttgtttgt ttgcttctga atgcaatgag aatttgtttg tgctcttgtc taataagact
    10921 gttgtgtctg tttttttgag tctcctgaag aaactggcaa ctgttgaact tcgctcactt
    10981 cctgaatgcg gatacgatag atgaaatgcc ctatagtgag tcgtattaca aggtaccccg
    11041 gatatagttc ctcctttcag caaaaaaccc ctcaagaccc gtttagaggc cccaaggggt
    11101 tatgct
//
