LOCUS       sequence-473981-       10136 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express HA-tagged NUP98::KDM5A in dual promoter lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10136)
  TITLE     Klco lab plasmids 2025
REFERENCE   2  (bases 1 to 10136)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CCLMPC_HA-NUP98::KDM5A
FEATURES             Location/Qualifiers
     source          1..10136
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     9..29
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11..29
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        130..509
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        510..713
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     664..684
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             728..908
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    955..1080
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1573..1806
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1991..2035
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2332..2449
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    3052..3076
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3100..3126
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    complement(5300..5324)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5353..5852
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5388..5411)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5785..5804
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      5873..5882
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5879..6589
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6024..6042)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6292..6311
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     LTR             6711..6944
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7016..7137
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7053..7072)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7107..7126
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7177..7312
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7332..7351)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7333..7351)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7361..7378)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7361..7377)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7370..7392)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7519..7974
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7606..7625)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7816..7837
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8000..8104
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8105..8173,8174..8965)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8323..8342)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9136..9724
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9625..9644
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9878..9895
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10012..10033
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10048..10053,10054..10071,10072..10078)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10086..10102
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10091..10113
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10110..10126
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10110..10126
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttggccat
       61 tgcatacgtt gtatccatat cataatatgt acatttatat tggctcatgt ccaacattac
      121 cgccatgttg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
      181 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
      241 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
      301 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
      361 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
      421 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
      481 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
      541 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
      601 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
      661 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctcgtttag
      721 tgaaccgggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
      781 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
      841 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
      901 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
      961 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
     1021 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
     1081 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
     1141 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
     1201 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     1261 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     1321 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     1381 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
     1441 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     1501 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     1561 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     1621 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     1681 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     1741 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     1801 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     1861 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     1921 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     1981 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     2041 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     2101 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     2161 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
     2221 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
     2281 atctcgacgg tatcgataag ctaattcaca aatggcagta ttcatccaca attttaaaag
     2341 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     2401 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta
     2461 cagggacagc agagatccag tttgggaatt agcttgatcg attagtccaa tttgttaaag
     2521 acaggatatc agtggtccag gctctagttt tgactcaaca atatcaccag ctgaagccta
     2581 tagagtacga gccatagata gaataaaaga ttttatttag tctccagaaa aaggggggaa
     2641 tgaaagaccc cacctgtagg tttggcaagc taggatcaag gttaggaaca gagagacagc
     2701 agaatatggg ccaaacagga tatctgtggt aagcagttcc tgccccggct cagggccaag
     2761 aacagttgga acagcagaat atgggccaaa caggatatct gtggtaagca gttcctgccc
     2821 cggctcaggg ccaagaacag atggtcccca gatgcggtcc cgccctcagc agtttctaga
     2881 gaaccatcag atgtttccag ggtgccccaa ggacctgaaa tgaccctgtg ccttatttga
     2941 actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct tctgctcccc gagctcaata
     3001 aaagagccca caacccctca ctcggcgcga tctagatctc gaatcgaatt cacaagtttg
     3061 tacaaaaaag caggctccgc ggccgccccc ttcaccatgt atccgtatga tgtgccggat
     3121 tatgcgttta acaaatcatt tggaacaccc tttgggggtg gcacaggtgg ctttggcaca
     3181 acttcaacat ttggacagaa tactggcttt ggcactacta gtggaggggc atttggaaca
     3241 tctgcatttg gttctagcaa caatactgga ggcctctttg gaaattcaca gactaaacca
     3301 ggaggattgt ttggaaccag ttcatttagc cagccagcta cctccacaag cactggcttt
     3361 gggtttggta cgtcaacagg aacagcaaat accttgtttg gaactgcaag cacagggacc
     3421 agtctcttct catcccaaaa caatgccttt gcacaaaata aaccaactgg ctttggcaat
     3481 tttggaacca gtactagcag tggaggactc tttggaacca caaataccac ctctaatcct
     3541 tttggcagca catctggctc cctctttggg ccaagtagtt ttacagctgc tcctactggg
     3601 actactatta aatttaaccc tccaactggt acagatacta tggtcaaagc tggagttagc
     3661 actaacataa gtaccaagca ccagtgtatt actgctatga aagaatatga aagcaagtca
     3721 ctagaggaac ttcgtttaga ggattatcag gctaacagga agggcccaca gaaccaggtg
     3781 ggagcaggta ccacaactgg cttgtttggg tcttctccag ccacttccag cgcaacagga
     3841 ctcttcagct cctccaccac taattcaggc tttgcatatg gtcagaacaa aactgccttt
     3901 ggaactagta caactggatt tggaacaaat ccaggtggtc tctttggcca acagaatcag
     3961 cagaccacca gcctcttcag caaaccattt ggccaggcta caaccaccca gaacactggc
     4021 ttttcctttg gtaataccag caccatagga cagccaagca ccaacaccat gggattattt
     4081 ggagtaaccc aagcctcaca gcctggaggt ctttttggga cagctacaaa caccagcact
     4141 gggacagcat ttggaacagg aacaggtctc tttgggcaga ccaatactgg atttggtgct
     4201 gttggttcga ccctgtttgg caataacaag cttactacat ttggaagcag cacaaccagt
     4261 gcaccttcat ttggtacaac cagtggcggg ctctttggtt ttggcacaaa taccagtggg
     4321 aatagtattt ttggaagtaa accagcacct gggactcttg gaactgggct tggtgcagga
     4381 tttggaacag ctcttggtgc tggacaggca tctttgtttg ggaacaacca acctaagatt
     4441 ggagggcctc ttggtacagg agcctttggg gcccctggat ttaatactac gacagccact
     4501 ttgggctttg gagcccccca ggccccagta gctttgacag atccaaatgc ttctgctgcc
     4561 cagcaggctg ttctccagca gcacatcaat agtctaacat actcaccttt tggagactct
     4621 cctctcttcc ggaatccgat gtcagaccct aagaagaagg aagaggatga cagcatggaa
     4681 gagaaaccac taaaagtgaa aggaaaggac tcttcagaga agaaacggaa acggaagcta
     4741 gaaaaggtag agcaactttt tggagaagga aaacagaagt ccaaggagtt aaagaaaatg
     4801 gacaaaccta gaaagaagaa attaaaatta ggtgcagaca aatcaaagga gctgaataaa
     4861 ctggccaaga aactagcaaa agaagaagag agaaagaaaa agaaggagaa ggctgctgca
     4921 gccaaagttg aacttgtgaa agagagcact gaaaagaaaa gagagaaaaa ggtgctggac
     4981 atcccctcaa agtatgactg gtcaggagca gaggagtctg atgatgagaa tgctgtgtgc
     5041 gcagcacaga actgccaaag gccctgcaag gacaaggtag actgggtaca atgtgatggt
     5101 ggctgtgatg agtggtttca tcaagtttgt gtgggtgtat ctccagaaat ggctgaaaat
     5161 gaagattaca tctgtataaa ctgtgcaaag aagcaggggc cagttagccc aggtccagca
     5221 ccacctcctt ccttcataat gagctacaaa ctaccaatgg aggatcttaa agagaccagt
     5281 tagaagggtg ggcgcgccga cccagctttc ttgtacaaag tggtgatatc ggtaccgagc
     5341 tcgaattcta ccgggtaggg gaggcgcttt tcccaaggca gtctggagca tgcgctttag
     5401 cagccccgct gggcacttgg cgctacacaa gtggcctctg gcctcgcaca cattccacat
     5461 ccaccggtag gcgccaaccg gctccgttct ttggtggccc cttcgcgcca ccttctactc
     5521 ctcccctagt caggaagttc ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa
     5581 tggaagtagc acgtctcact agtctcgtgc agatggacag caccgctgag caatggaagc
     5641 gggtaggcct ttggggcagc ggccaatagc agctttgctc cttcgctttc tgggctcaga
     5701 ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg
     5761 cccgaaggtc ctccggaggc ccggcattct gcacgcttca aaagcgcacg tctgccgcgc
     5821 tgttctcctc ttcctcatct ccgggccttt cgaccatcta gatccaccgc gggccaccat
     5881 ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc gcttcaaggt
     5941 gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg gcgagggccg
     6001 cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc ccctgccctt
     6061 cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg tgaagcaccc
     6121 cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt gggagcgcgt
     6181 gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc tgcaggacgg
     6241 cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg gccccgtaat
     6301 gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg aggacggcgc
     6361 cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact acgacgctga
     6421 ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct acaacgtcaa
     6481 catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac agtacgaacg
     6541 cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagtaga gcggccaact
     6601 cgagggatcc cccggggtcg actgatcaaa ttcgagctcg gtacctttaa gaccaatgac
     6661 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     6721 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
     6781 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     6841 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     6901 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca
     6961 tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggaactt
     7021 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa
     7081 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca
     7141 tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     7201 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     7261 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctagggacg
     7321 tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac
     7381 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     7441 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
     7501 gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     7561 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     7621 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     7681 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     7741 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     7801 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     7861 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     7921 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg
     7981 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     8041 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     8101 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     8161 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     8221 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     8281 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     8341 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     8401 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     8461 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     8521 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     8581 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     8641 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     8701 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     8761 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     8821 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     8881 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     8941 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     9001 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     9061 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     9121 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     9181 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     9241 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     9301 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     9361 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     9421 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     9481 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     9541 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     9601 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     9661 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     9721 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     9781 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     9841 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     9901 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     9961 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
    10021 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
    10081 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacg
//
