LOCUS       sequence-474229-       14094 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Reports Cur activity in Bacteroides thetaiotaomicron.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14094)
  AUTHORS   Kabonick S, Verma K, Modesto JL, Pearce VH, Winokur KM, Groisman EA,
            Townsend GE
  TITLE     Hierarchical glycolytic pathways control the carbohydrate 
            utilization regulator in human gut Bacteroides.
  JOURNAL   bioRxiv [Preprint]. 2024 Nov 13:2024.11.13.623061. doi: 
            10.1101/2024.11.13.623061.
  PUBMED    39605394
REFERENCE   2  (bases 1 to 14094)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            [Preprint]. 2024 Nov 13:2024.11.13.623061. doi: 
            10.1101/2024.11.13.623061."
COMMENT     Sequence Label: P-fusA2
FEATURES             Location/Qualifiers
     source          1..14094
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(2055..3497)
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPEGDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MQHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     primer_bind     complement(3880..3897)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3880..3896)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3889..3911)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(6813..6832)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6932..6954
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6992..7010)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9721..9825
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9826..9894,9895..10686)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10044..10063)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10857..11445
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11346..11365
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11599..11616
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11733..11754
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11769..11774,11775..11792,11793..11799)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11807..11823
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11812..11834
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11831..11847
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11831..11847
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             complement(12881..13960)
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLL"
ORIGIN
        1 cgtattgacg cgacgtaaaa tttcaacgag cacgccggga tacttaccat attctctgct
       61 aattatcccg acatcatcgg taacaataaa tgctggataa ctggttgctg acgcatccat
      121 ataactcatc aaccccggcg ttccatcagg tacaggtttc aacgtttcag gatcaagcgc
      181 tcgcgcatat acccacggcg gaacatgttt acgctgcatt tcatcctcaa agaaacaagt
      241 gttgagttca acttgattaa atatatctcg gatctgacta atatcactga gattgaaagt
      301 atcaaataaa agatgattga aatcatcacg tttcagagat tctttttcgt aacttttcca
      361 gccgcctccg gttatgatat aaaggctttt atctccagaa aatgagattt ttttatcttt
      421 catataatgg cagagtaaat aaataaagta tggcgaacca ataagacaaa gatctttccc
      481 ttgatttttt attcgttcaa gactattcaa tgttttaaca aaatctattc gttcttctgt
      541 tacggtaaat gtcgtaggat ataacaattc caccaaactc ataacatatt taaaccaaat
      601 attatgagca ttaaatctat ctggtcccaa attgactaat tctatttgat gatcaaacca
      661 actaccaaca tatttcatgc cataactcac agagcctaag agtctctcaa tacttaatct
      721 gtcacgcgcc acctgacttt ttaaaccatt cgtgccgcta ctggtaaacc aactttcaat
      781 ctcgttttcc tgagaagtta ataagcgagt aaacttaaaa accgatgttg ggaatacagg
      841 tatgtcatca atttccgtaa tattgtcatc tactttgtgt gcctgacagt agtgacgata
      901 ttctcgacaa tgtttataat gattacgaaa tgcatcaagc acaagtttct ttctgatttt
      961 ttcctgctcg tcgtaagacc acactaatgg atcgctcgaa aaaatcaaat catcaatttc
     1021 tgagcttgct gtaatttctt gtttatcaac atatgaagtc atattcttta tcctcctcct
     1081 tattaagaca gcgaaatcgc ttgattttca atctcaattc tcattcggcg ttcattgact
     1141 gtcgcaatag ttaaatgttc aaatgacggt tcagtaatat caacatcaat atccagatga
     1201 tcattatcca tcgcgatagc ggctttcgta accgattgat aaaaattgcg caggaccact
     1261 aaattttcac tcaagtcatg cgaacttcct aacaaagaat atatcttgca tcgattacta
     1321 cgaatatttg ataacaatgt gataacttca tcttgcttga cccaattatc gttatttgca
     1381 gtaaaagcaa taaacggtat atcaagatac atcatgttat taattgtaga agctaaatct
     1441 tcccaaccaa aatcaagaca atctctcgca aagacttcag cacccaattt atggccttca
     1501 aaatctagat tattcggcaa ttcattaatg ggtagactga gataatcaaa ccctaaagct
     1561 ctttcaagag aatatcttaa gttaacaaca ccgactgcgg tgattaaaaa cgaagcattg
     1621 atttcagata ggcttgcata agctatccgc gcagataagc ttgaagccaa cataccgaag
     1681 ttatttattt ttcgtgtagt taaccaatca accactgcta acaagctctg ctttcctata
     1741 gacattgtaa attcatcaat tgtccctgaa ctcaatccaa cgtggtgaag cgaatcatag
     1801 cggatcacat gaaatccatt ccgcgataaa tattccgcca gaccagcaaa atgatccatc
     1861 ctgcgggcaa aaccagacgc aataataatg gcattctttc tctttgggct gttttcttct
     1921 ggcagcgttt cccaaacatg aattttttta tttccttcaa cacaaataac gtggtcgatg
     1981 gttttatatt tggatttatt ttccattaat atattactat tttacgattt acaatttaca
     2041 atttgccatg cggattacgg gacaaataca aggaacttat cttcttccag gaatcgagtc
     2101 tgttctattt caaccgcaac atccttagcc gtatagttag atggcctttc atgagaaata
     2161 tatgtcacta atcgttgcaa cggtctcatt ccgtcatgag atccaccaac tcgaaatatg
     2221 ttattcattc ctgcttctac aatcctttcc gcacctttta atgctaacgc atctcgatat
     2281 ttaaatgatg actcccaagg aaaaatagat atggtttgcg tcttattttt ttgaacataa
     2341 ggcaatattt gctcaatatt atcgacgtga tgaaggtaca cacatctgcc aagtggttga
     2401 ttaaattcca cacctgcatt tgactcaata atcatccaac gttgatgaat atccacctct
     2461 acttttaatc cagcaaacaa gctttctttt tgaactaaag aataggccgc cttttcatca
     2521 aaatcttttt tggcattcgg taatatatgc gcatatagat taagtttttc tatcaacgct
     2581 aacttaaatt cctcataatg atttcccatg taatatatgt tttgggcaga aaaacaagct
     2641 cgctgatcgt aaaaacaaac atcatgagcc gcacctgtcg ctgcggacgt caaatcaaca
     2701 ggattatcga taatgcaaag actcttttta gaaccaaatt taatcacatc agcataagat
     2761 ggcgcatgct ctaccgccca attaatcgca tctggccctc cccaagcgac aataacatcc
     2821 gcatgttgca taatttcttt tgcgagtgat gtatcacctt ggtggggcca atatataaca
     2881 gataaagagc gcgttatcgg atgattaggg tctacatcaa taaaacttaa cgctaatgca
     2941 ttagcggtaa aaggatcggt tgacgatgtt tttataatac actgattctt agttaaaatt
     3001 gcgcgtaata tagacatgat cccagataat ggaacattac ctgccaacag atgtacagat
     3061 ttacctttcg gaaaagcccg aacataactt tcatcctgag gtagccattc atccatgata
     3121 tggcgagaac caagttcatt ttctacaaca tcataaaggc cgcctttaga acataaaatc
     3181 atagatatcc aattggcctc tagcttagcc atttcttctg aatatcccat atattttttt
     3241 aagtcacgaa tgtatgtcct gcgtcttgag tattcttcat ttttccatct ttgccctacc
     3301 gtatagagaa aattgacaat gttatgcaac cgtaattcgt tatttccatt acaatcaata
     3361 atgtttttta catgagagtc attcaatatt ggcaggtaaa cactattatc accaaaatta
     3421 atggattgca ctaaatcatc accttcggga aagatttcaa cctggccgtt aataatgaat
     3481 gaaatttttt tagtcatatt cgtttaatta aataaataat ttattttatt ttaaaatgcg
     3541 ggagtgacta gtaatatata agattttagt aattactcag tatgtttctc gcataccatt
     3601 aatttagtga tgcgcaaatt agggatttat tgggggaaaa gcaatattta tgacagtgtt
     3661 actaaacaat gttatgtaac ataaggtccg ttttatgggt agcagacagg ccagacttat
     3721 acctaattcc cgttaattgt atcaattgct cagaaaaaag gtttaaattt gcaggctata
     3781 atcaattggt aacaagctaa acatctacta tggcaggtat ttaccttcat attcccttct
     3841 gtaagacacg ttggatcccc gggtaccgag ctcgaattca ctggccgtcg ttttacaacg
     3901 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     3961 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     4021 cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
     4081 acaccgcata cacaccataa acttttttta gaataagcac acaaccgttt tccgaaccct
     4141 gcaaaatgtt ttctgaatcc gaacggtgta acactccatt gagagaggct gccgtttggt
     4201 cgctccccct ttgggggcgg gggggggtta catacccatg ccgaaacctc tgcttctggt
     4261 gatttgcttg aataggtctt tcccctcttc catagctttt gatatgtttg ggaaatgatg
     4321 ccttaaagcc tccagttgtt cggaattgaa caagtctttc atcttaccaa gttctttttt
     4381 caactccttg gtttcggctt ttagtttttg gttctccgtc cttaataggt tactggttgt
     4441 ccttgcgttg tccatttgtt gtctataata ctccttgtca ttctcggctt tgaatgcctt
     4501 tgtgctgttt cgctcttttt caagtatagc ctttcccagt ctatcggata gttgttcatt
     4561 ttccccctct aaagtcttta ctttggcttt taaggcatcc ttttccctat cgttgactgt
     4621 ttttccaatc aagccgtaaa acttctctga agccttagaa atgagttttt ggacgttctt
     4681 ctttgtttca atggaacgta gttccttctg aagctgaaga agctggtttt gtgcgtcctt
     4741 gtatttgtct aatgcactgg atatatcgtt ggatagttcc tgaagctgtt ctttcgcaca
     4801 ttcggtcttg tactgcatag ccgataagtg tttgcggtca gaagaaacgc cacgttccat
     4861 gcccagtgtt tcagatgcta tggtttggag ttctgccatg tcatcacgcg ataaacgcac
     4921 acttttccca ttcggctgcg tccaatcgaa aactacatgg gcatgaaggt taggtgtcca
     4981 ctgctttgcg ttcatgtatc cttcgtcctt gtgtatatgg atttgaaacg cttcgatacc
     5041 gaaacgttct ttgcagaccg tggcaaactg ctggagttcc tgcatagtgg tttcttgttt
     5101 gattactatt actccctctc gtatgggtgc ggctttagcc tgcatcttct gcccaaccgt
     5161 atcgagatat ctttgttttg cactctccag ccgatgggaa atgctatctc caacccagct
     5221 ttcattcaaa tgactaagtt cgggacgaac atagtccaac tctttttccc taaagttgtg
     5281 aatctcgctc cccggcttca ctgcttgtac atgaatactt gttgctccca taagttaaca
     5341 tttttgtgac aatcgataac agccggtgac agccggctga cagggggtta agggggcttg
     5401 tccccttaca cacgcactct ttagggtgct agtgtgctat caccatactg cataggtgcg
     5461 aagttagtga atgttttgta aatgcacaaa taaagggaaa aacatttgga tttgcgataa
     5521 taaagtacta cctttgttgc tgaccaaacg gtagctgacc gatacgggag agttaccaaa
     5581 atacaagccg ctggagttaa ttgacggaca tccgacatct ccagcggctt tatttttgcc
     5641 tatctgcttc gcctaggcac accagtacct ctactaaaaa tgtacttcaa agatacttat
     5701 tttctaccga cttgatagtt tttaccccat attcttggac atttttcccc catgaggtta
     5761 tctttgtagg gtgaaagaga aacccataaa cggggataga ttgaatgctg ggaagcataa
     5821 acaatcgggg taaggttagc gaaccttgcc tttcatcccc cattataact ttacatagag
     5881 gaactttatc tatccccccc cgcccccaaa gggggagcga ccaaacggca gcttcactca
     5941 atggagtgtt actgttcatc aaagccaagt gataattgtc gtttctctgc ttcttctttc
     6001 ttttgggcag ctaaagtctt tttccgaacg tatgttttag caaatgtcac tcggtcacca
     6061 ttgaatacta tcagaggatt aataaaccaa agattatcgg ctggtcctcg ggctatgatt
     6121 tcagctttta caagttctgc aagtccttta taaacggctt tgtctgtttt gtatttggta
     6181 tattctaggc atttttttct attgaaaatg attaaatcat ttttgggttt catgcaggtc
     6241 ataaagtaac caaaaacccg aatagctgct tgtgataggt caaagaatgc agcaaagtta
     6301 gaaagataca atttagtgaa ttgttcttca tctacttcta tttgacggat aaacgaagtc
     6361 ttaaacactt ctccagtttc agtgtcggct aaagctacta cagctctctt atcgccacca
     6421 ctattactct tatacttttt aacaacatga ttttcaatac cttctatagc ttgtttcata
     6481 aaaggatttt cttcgttctt ttgaaaatcg gttaacttaa ctgctttttt attttccatt
     6541 ttgatatgtt tttgggaaat attattctcc acaaagtaaa ctattatttt ccataaaaac
     6601 aatattaagg gaaatattat tttcctattt agtatcatat taggaaatcg gtattttcta
     6661 gattggaaaa tgagaatttc caatatggaa aatgccctat attgtgtatc aagtacttaa
     6721 cttattctat ttcttttatt cttaatatac ccccaaaaca gcacaaaatc agtcacttaa
     6781 aaatcatcgg tcggggaatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     6841 agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
     6901 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
     6961 ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt
     7021 aatgtcatga taataatggt ttcttagcta aatttaaata taaacaacga attatctcct
     7081 taacgtacgt tttcgttcca ttggccctca aaccccgtta tatacattca tgtccattta
     7141 tgtaaaaaat cctgctgacc ttgtttatgt cttgtcagtc accatttgca aaaccatatt
     7201 tgaccctcaa agaggctgaa tttgataagc aacttgctac atactcataa taaggagcta
     7261 aatagaacac gaatgggaaa tactcaaatg ccaaactaaa gaagatattg gccaaaataa
     7321 acgctatacc gagagagaaa cttgattttt caacttccta aaacagtgtt gttcaaacat
     7381 ttctacttat ttgtacttac cagttgaacc tacgtttccc taataaaatg tctatggtaa
     7441 aaagttaaaa aatcctccta cttttgttag atatattttt ttgtgtaatt ttgtaatcgt
     7501 tatgcggcag taataatata catattaata cgagttagga atcctgtagt tctcatatgc
     7561 tacgaggagg tattaaaagg tgcgtttcga caatgcatct attgtagtat attattgctt
     7621 aatccaaatg aatattataa atttaggaat tcttgctcac attgatgcag gaaaaacttc
     7681 cgtaaccgag aatctgctgt ttgccagtgg agcaacggaa aagtgcggct gtgtggataa
     7741 tggtgacacc ataacggact ctatggatat agagaaacgt agaggaatta ctgttcgggc
     7801 ttctacgaca tctattatct ggaatggtgt gaaatgcaat atcattgaca ctccgggaca
     7861 catggatttt attgcggaag tggagcggac attcaaaatg cttgatggag cagtcctcat
     7921 cttatccgca aaggaaggca tacaagcgca gacaaagttg ctgttcaata ctttacagaa
     7981 gctgcaaatc ccgacaatta tatttatcaa taagattgac cgagccggtg tgaatttgga
     8041 gcgtttgtat ctggatataa aagcaaatct gtctcaagat gtcctgttta tgcaaaatgt
     8101 tgtcgatgga tcggtttatc cggtttgctc ccaaacatat ataaaggaag aatacaaaga
     8161 atttgtatgc aaccatgacg acaatatatt agaacgatat ttggcggata gcgaaatttc
     8221 accggctgat tattggaata cgataatcgc tcttgtggca aaagccaaag tctatccggt
     8281 gctacatgga tcagcaatgt tcaatatcgg tatcaatgag ttgttggacg ccatcacttc
     8341 ttttatactt cctccggcat cggtctcaaa cagactttca tcttatcttt ataagataga
     8401 gcatgacccc aaaggacata aaagaagttt tctaaaaata attgacggaa gtctgagact
     8461 tcgagacgtt gtaagaatca acgattcgga aaaattcatc aagattaaaa atctaaaaac
     8521 tatcaatcag ggcagagaga taaatgttga tgaagtgggc gccaatgata tcgcgattgt
     8581 agaggatatg gatgattttc gaatcggaaa ttatttaggt gctgaacctt gtttgattca
     8641 aggattatcg catcagcatc ccgctctcaa atcctccgtc cggccagaca ggcccgaaga
     8701 gagaagcaag gtgatatccg ctctgaatac attgtggatt gaagacccgt ctttgtcctt
     8761 ttccataaac tcatatagtg atgaattgga aatctcgtta tatggtttaa cccaaaagga
     8821 aatcatacag acattgctgg aagaacgatt ttccgtaaag gtccattttg atgagatcaa
     8881 gactatatac aaagaacgac ctgtaaaaaa ggtcaataag attattcaga tcgaagtgcc
     8941 gcccaaccct tattgggcca caatagggct gactcttgaa cccttaccgt tagggacagg
     9001 gttgcaaatc gaaagtgaca tctcctatgg ttatctgaac cattcttttc aaaatgccgt
     9061 ttttgaaggg attcgtatgt cttgccaatc cgggttacat ggatgggaag tgactgatct
     9121 gaaagtaact tttactcaag ccgagtatta tagcccggta agtacacctg ctgatttcag
     9181 acagctgacc ccttatgtct tcaggctggc cttgcaacag tcaggtgtgg acattctcga
     9241 accgatgctc tattttgagt tgcagatacc ccaagcggca agttccaaag ctattacaga
     9301 tttgcaaaaa atgatgtctg agattgaaga catcagttgc aataatgagt ggtgtcatat
     9361 taaagggaaa gttccattaa atacaagtaa agactatgca tcagaagtaa gttcatacac
     9421 taagggctta ggcattttta tggttaagcc atgcgggtat caaataacaa aaggcggtta
     9481 ttctgataat atccgcatga acgaaaaaga taaactttta ttcatgttcc aaaaatcaat
     9541 gtcatcaaaa taatggagcg gtcaggaaat ttctataagg caatacagtt gggatatata
     9601 cttatctcca ttcttatcgg atgtatggca tataatagcc tctatgaatg gcaggagata
     9661 gaagcattag aacttggcaa taaaaaaata gacgcaggtg gcacttttcg gggaaatgtg
     9721 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     9781 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     9841 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     9901 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     9961 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
    10021 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
    10081 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
    10141 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
    10201 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
    10261 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
    10321 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
    10381 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
    10441 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
    10501 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
    10561 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
    10621 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
    10681 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
    10741 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
    10801 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
    10861 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
    10921 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
    10981 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
    11041 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
    11101 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
    11161 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
    11221 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
    11281 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
    11341 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
    11401 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
    11461 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
    11521 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
    11581 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
    11641 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
    11701 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
    11761 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
    11821 caatttcaca caggaaacag ctatgaccat gattacgcca agcttgcatg cctgcaggtc
    11881 gacttacatg tggtactttt taatattatc atcaacaata ttgattacat ttttttggct
    11941 catcaaatca ttcattggtt caaaggacag caatacactt ttcgcaccac acttttcaat
    12001 tgccaactta gccgcagtta tacactccgt ataatttccg acagcgtttt ctgcaattat
    12061 ttcttcaagt ttattttcga aattttcatt agggtgcatt tcaagaacat aatcactaat
    12121 aaatgcacgc gtctcttgtt tagctttatt actatcttcg ttatagttaa ctaatatcat
    12181 taactgatgg tctatctctg ataggtcaac gtcatattta tccgcaacgg ctttatatct
    12241 ttcagcatat tcatatctaa catcattaga atcatcccac ttaaagatga gaggaatacc
    12301 ttttttggcc gcccactcaa caatatgatg actggttgct gttacatatt tccgaggtcc
    12361 gcctggcgta taagcatggg gatttacaga tattttaggg aagctataaa aatcgttatc
    12421 tggattacaa tagcctgttg ttaaagcatc gttaatgatt tcataacact cttcaaatag
    12481 ttgctgttga tattcaaccg ggcgattaaa aaaatgcatt tcatcttttt tttcgcaatc
    12541 actaaaccct aaaataaatc tcccttcact taactgatcc aataagcaag cttcctccgc
    12601 tatgcggaca ggatgatgag ttgtaatgat gtgatttaat gaaccaattt taattttctc
    12661 tgttaaaccg agcagaaaac cagaaacagt cagaggagcg ccgacaacac cattatctga
    12721 aaaatgattt tcatacacta aaatctgttc aaaattcaac ttatcaacat actccgttat
    12781 ttcctgcatg cgaactatac tttgttcttg aacagttgtt gaattgatga agttaaggaa
    12841 gaacaatcca aatttcatat ccattgtctt attcctttct ttataatagc gaacgttgtt
    12901 tttctttaag aaatggcatg acatcagact ggaagagctt catggaagca ataatttcgt
    12961 ctactgttcc attagcttca aatccacaac aaatatttga tattcctgta gcatcaatgt
    13021 ctttttgaat tatgtcaata cattcctgcg gcgttcccac gggattgatt tcgtaactgt
    13081 aatcaatacg gcgattagta tctttatgtc cttttaatac aaagtcacgc cactgccctt
    13141 tattgaaatc ataacctctt gtttggtctg aatcatcaaa aatagtcgta gcattcacat
    13201 aagaatcata ccaatgcccc agaaatttcc ggcaaatctc tttcgcttta attgagtcat
    13261 gatctacaga tgttatatat gataagcaat ggtcgatatt atgaatatcg tgcccatatt
    13321 cttgagccac ttcattataa agctcaagtt gtgctttctt ttcgttagta tttataatcc
    13381 aacttaatat catcggtagg ccaaattgag cagcccactc agtcgtcgaa gctgattcag
    13441 ccaccacata aaccggtgcg ccacctctgc tatacgccgc ggggtttact tttaccttat
    13501 ggaacttgat atgttcatta tcagcttcca tatatccctc tgtcatgcca ttctttatca
    13561 gcccgtacca gcattccgct aaggcgcgac tgttattcat atctgtgccg aatacgcgaa
    13621 agtccttgtt gtaaagccct cggcaaatac caaaccgaaa tcgtcctttt gacatttgat
    13681 ccaataaatt cacatcttca agttggcgta ctggatgggc tgtgggaaga acaatagcgg
    13741 cagttcctac attcaatttt ttagtcgcgc caagtaaata tgcagcagcg acataagggt
    13801 taccaagcaa accaaactcc gtgaaatgat gctccagtaa ccatacggta tcaaaaccac
    13861 actcctcaga gatgcgacct aatttaacca aacgtttcat tacctctgtt tgagaaaatt
    13921 ggggaggttg gtatgtaagc aaaaagtttc caaatttcat atattttaat taaaaattaa
    13981 gaatgaagaa tttcgagttt atgagtgtta ccttcattca tccttcttca cccttcattt
    14041 atatcaacta ttaaatgctt ggtttaagct taaagcacac cctttctgcg tcct
//
