LOCUS       sequence-47432-e        9124 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9124)
  AUTHORS   Euskirchen P, Skaftnesmo KO, Huszthy PC, Brekka N, Bjerkvig R, 
            Jacobs AH, Miletic H
  TITLE     NUMB does not impair growth and differentiation status of 
            experimental gliomas.
  JOURNAL   Exp Cell Res. 2011 Dec 10;317(20):2864-73. Epub 2011 Sep 12.
  PUBMED    21939656
REFERENCE   2  (bases 1 to 9124)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Exp Cell 
            Res."; date: "2011-12-10"; volume: "317(20)"; pages: "2864-73. Epub 
            2011 Sep 12"
COMMENT     Sequence Label: NUMB2-eGFP-M107K
FEATURES             Location/Qualifiers
     source          1..9124
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1836..1845
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1842..1844,1845..1847,1848..2561)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1842..1844,1845..1847,1848..2558)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1887..1908)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2148..2167)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2495..2516
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2577..3165
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2630..2650)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3048..3059)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3251..3484
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3556..3677
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3593..3612)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3647..3666
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3696..3831
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3863..3882)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3864..3882)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3892..3909)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3892..3908)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3901..3923)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4053..4508
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4140..4159)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4350..4371
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4537..4641
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4642..4710,4711..5502)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4860..4879)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5673..6261
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6162..6181
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6415..6432
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6549..6570
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6585..6590,6591..6608,6609..6615)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6623..6639
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6628..6650
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6647..6663
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6647..6663
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6682..6702
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6684..6702
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     LTR             6958..7138
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7182..7307
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7800..8033
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8217..8261
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        8540..8843
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8844..9047
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8998..9018
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     9044..9068
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gatctgccac catgaacaaa ttacggcaaa gttttaggag aaagaaggat gtttatgttc
       61 cagaggccag tcgtccacat cagtggcaga cagatgaaga aggcgttcgc accggaaaat
      121 gtagcttccc ggttaagtac cttggccatg tagaagttga tgaatcaaga ggaatgcaca
      181 tctgtgaaga tgctgtaaaa agattgaaag ctgaaaggaa gttcttcaaa ggcttctttg
      241 gaaaaactgg aaagaaagca gttaaagcag ttctgtgggt ctcagcagat ggactcagag
      301 ttgtggatga aaaaactaag gacctcatag ttgaccagac gatagagaaa gtttctttct
      361 gtgccccaga caggaacttt gatagagcct tttcttacat atgccgtgat ggcaccactc
      421 gtcgctggat ctgtcactgc ttcatggctg tcaaggacac aggtgaaagg ttgagccatg
      481 cagtaggctg tgcttttgca gcctgtttag agcgcaagca gaagcgggag aaggaatgtg
      541 gagtgactgc tacttttgat gctagtcgga ccacttttac aagagaagga tcattccgtg
      601 tcacaacagc cactgaacaa gcagaaagag aggagatcat gaaacaaatg caagatgcca
      661 agaaagctga aacagataag atagtcgttg gttcatcagt tgcccctggc aacactgccc
      721 catccccatc ctctcccacc tctcctactt ctgatgccac gacctctctg gagatgaaca
      781 atcctcatgc catcccacgc cggcatgctc caattgaaca gcttgctcgc caaggctctt
      841 tccgaggttt tcctgctctt agccagaaga tgtcaccctt taaacgccaa ctatccctac
      901 gcatcaatga gttgccttcc actatgcaga ggaagactga tttccccatt aaaaatgcag
      961 tgccagaagt agaaggggag gcagagagca tcagctccct gtgctcacag atcaccaatg
     1021 ccttcagcac acctgaggac cccttctcat ctgctccgat gaccaaacca gtgacagtgg
     1081 tggcaccaca atctcctacc ttccaaggga ccgagtgggg tcaatcttct ggtgctgcct
     1141 ctccaggtct cttccaggcc ggtcatagac gtactccctc tgaggccgac cgatggttag
     1201 aagaggtgtc taagagcgtc cgggctcagc agccccaggc ctcagctgct cctctgcagc
     1261 cagttctcca gcctcctcca cccactgcca tctcccagcc agcatcacct ttccaaggga
     1321 atgcattcct cacctctcag cctgtgccag tgggtgtggt cccagccctg caaccagcct
     1381 ttgtccctgc ccagtcctat cctgtggcca atggaatgcc ctatccagcc cctaatgtgc
     1441 ctgtggtggg catcactccc tcccagatgg tggccaacgt atttggcact gcaggccacc
     1501 ctcaggctgc ccatccccat cagtcaccca gcctggtcag gcagcagaca ttccctcact
     1561 acgaggcaag cagtgctacc accagtccct tctttaagcc tcctgctcag cacctcaacg
     1621 gttctgcagc tttcaatggt gtagatgatg gcaggttggc ctcagcagac aggcatacag
     1681 aggttcctac aggcacctgc ccagtggatc cttttgaagc ccagtgggct gcattagaaa
     1741 ataagtccaa gcagcgtact aatccctccc ctaccaaccc tttctccagt gacttacaga
     1801 agacgtttga aattgaactt gcagatccac cggtcgccac catggtgagc aagggcgagg
     1861 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     1921 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     1981 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
     2041 acggcgtgca gtgcttcagc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     2101 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     2161 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     2221 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactaca
     2281 acagccacaa cgtctatatc atggccgaca agcagaagaa cggcatcaag gtgaacttca
     2341 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     2401 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtccg
     2461 ccctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     2521 ccgccgggat cactctcggc atggacgagc tgtacaagta aagcggccgc gtcgacaatc
     2581 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     2641 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     2701 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     2761 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     2821 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     2881 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     2941 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct
     3001 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     3061 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     3121 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgaa tacgagctcg
     3181 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     3241 aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt
     3301 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     3361 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     3421 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     3481 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     3541 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     3601 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     3661 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccagtt
     3721 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     3781 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     3841 gcgtcgagac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
     3901 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     3961 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     4021 acagttgcgc agcctgaatg gcgaatggcg cgacgcgccc tgtagcggcg cattaagcgc
     4081 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     4141 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     4201 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     4261 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     4321 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     4381 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     4441 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt
     4501 tacaatttcc caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     4561 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     4621 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     4681 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     4741 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     4801 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     4861 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     4921 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     4981 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     5041 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     5101 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     5161 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     5221 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     5281 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     5341 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     5401 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     5461 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     5521 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     5581 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     5641 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     5701 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     5761 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     5821 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     5881 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     5941 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     6001 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     6061 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     6121 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     6181 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     6241 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     6301 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     6361 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     6421 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     6481 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     6541 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     6601 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     6661 gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg
     6721 caagcttaat gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt
     6781 agcaacatgc cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt
     6841 ggtacgatcg tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca
     6901 ctgaattgcc gcattgcaga gatattgtat ttaagtgcct agctcgatac aataaacggg
     6961 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     7021 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     7081 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     7141 ggcgcccgaa cagggacctg aaagcgaaag ggaaaccaga gctctctcga cgcaggactc
     7201 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     7261 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     7321 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     7381 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     7441 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     7501 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     7561 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     7621 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     7681 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     7741 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     7801 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcctcaatg
     7861 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     7921 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     7981 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     8041 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     8101 aataaatctc tggaacagat tggaatcaca cgacctggat ggagtgggac agagaaatta
     8161 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     8221 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     8281 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     8341 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     8401 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
     8461 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc
     8521 gataagcttg ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
     8581 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     8641 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     8701 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     8761 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     8821 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
     8881 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
     8941 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc
     9001 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
     9061 gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga caccgactct
     9121 agag
//
