LOCUS       sequence-474334-        4823 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Nuclear Envelope, Excitation: 487, Emission: 509.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4823)
  AUTHORS   Day RN, Davidson MW
  TITLE     The fluorescent protein palette: tools for cellular imaging.
  JOURNAL   Chem Soc Rev. 2009 Oct;38(10):2887-921. doi: 10.1039/b901966a. Epub 
            2009 Aug 4.
  PUBMED    19771335
REFERENCE   2  (bases 1 to 4823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1039/b901966a"; 
            journalName: "Chem Soc Rev"; date: "2009-10"; volume: "38"; issue: 
            "10"; pages: "2887-921"
COMMENT     Sequence Label: mEmerald-LaminB1-10
FEATURES             Location/Qualifiers
     source          1..4823
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      547..556
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(553..555,556..558,559..1269)
                     /codon_start=1
                     /product="brightly fluorescent monomeric variant of GFP"
                     /label=mEmerald
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(598..619)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(859..878)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1206..1227
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    3190..3311
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3227..3246)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3281..3300
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(3318..3773)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3455..3476)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3667..3686
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        3800..3904
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4061..4649
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4550..4569
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      541 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
      601 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
      661 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
      721 ccctggccca ccctcgtgac caccttgacc tacggcgtgc agtgcttcgc ccgctacccc
      781 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
      841 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
      901 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
      961 atcctggggc acaagctgga gtacaactac aacagccaca aggtctatat caccgccgac
     1021 aagcagaaga acggcatcaa ggtgaacttc aagacccgcc acaacatcga ggacggcagc
     1081 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     1141 cccgacaacc actacctgag cacccagtcc aagctgagca aagaccccaa cgagaagcgc
     1201 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     1261 ctgtacaagt ccggactcag atctcgagct caagcttcga tggcgactgc gacccccgtg
     1321 ccgccgcgga tgggcagccg cgctggcggc cccaccacgc cgctgagccc cacgcgcctg
     1381 tcgcggctcc aggagaagga ggagctgcgc gagctcaatg accggctggc ggtgtacatc
     1441 gacaaggtgc gcagcctgga gacggagaac agcgcgctgc agctgcaggt gacggagcgc
     1501 gaggaggtgc gcggccgtga gctcaccggc ctcaaggcgc tctacgagac cgagctggcc
     1561 gacgcgcgac gcgcgctcga cgacacggcc cgcgagcgcg ccaagctgca gatcgagctg
     1621 ggcaagtgca aggcggaaca cgaccagctg ctcctcaact atgctaagaa ggaatctgat
     1681 cttaatggcg cccagatcaa gcttcgagaa tatgaagcag cactcaattc gaaagatgca
     1741 gctcttgcta ctgcacttgg tgacaaaaaa agtttagagg gagatttgga ggatctgaag
     1801 gatcagattg cccagttgga agcctcctta gctgcagcca aaaaacagtt agcagatgaa
     1861 actttactta aagtagattt ggagaatcgt tgtcagagcc ttactgagga cttggagttt
     1921 cgcaaaagca tgtatgaaga ggagattaac gagaccagaa ggaagcatga aacgcgcttg
     1981 gtagaggtgg attctgggcg tcaaattgag tatgagtaca agctggcgca agcccttcat
     2041 gagatgagag agcaacatga tgcccaagtg aggctgtata aggaggagct ggagcagact
     2101 taccatgcca aacttgagaa tgccagactg tcatcagaga tgaatacttc tactgtcaac
     2161 agtgccaggg aagaactgat ggaaagccgc atgagaattg agagcctttc atcccagctt
     2221 tctaatctac agaaagagtc tagagcatgt ttggaaagga ttcaagaatt agaggacttg
     2281 cttgctaaag aaaaagacaa ctctcgtcgc atgctgacag acaaagagag agagatggcg
     2341 gaaataaggg atcaaatgca gcaacagctg aatgactatg aacagcttct tgatgtaaag
     2401 ttagccctgg acatggaaat cagtgcttac aggaaactct tagaaggcga agaagagagg
     2461 ttgaagctgt ctccaagccc ttcttcccgt gtgacagtat cccgagcatc ctcaagtcgt
     2521 agtgtacgta caactagagg aaagcggaag agggttgatg tggaagaatc agaggcgagt
     2581 agtagtgtta gcatctctca ttccgcctca gccactggaa atgtttgcat cgaagaaatt
     2641 gatgttgatg ggaaatttat ccgcttgaag aacacttctg aacaggatca accaatggga
     2701 ggctgggaga tgatcagaaa aattggagac acatcagtca gttataaata tacctcaaga
     2761 tatgtgctga aggcaggcca gactgttaca atttgggctg caaacgctgg tgtcacagcc
     2821 agccccccaa ctgacctcat ctggaagaac cagaactcgt ggggcactgg cgaagatgtg
     2881 aaggttatat tgaaaaattc tcagggagag gaggttgctc aaagaagtac agtctttaaa
     2941 acaaccatac ctgaagaaga ggaggaggag gaagaagcag ctggagtggt tgttgaggaa
     3001 gaacttttcc accagcaggg aaccccaaga gcatccaata gaagctgtgc aattatgtag
     3061 ggatccaccg gatctagata actgatcata atcagccata ccacatttgt agaggtttta
     3121 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     3181 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     3241 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     3301 aatgtatctt aacgcgtaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
     3361 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
     3421 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
     3481 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
     3541 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
     3601 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
     3661 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
     3721 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg
     3781 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     3841 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     3901 gagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     3961 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     4021 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     4081 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     4141 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     4201 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     4261 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     4321 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     4381 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     4441 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     4501 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     4561 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     4621 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     4681 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     4741 ataaccgtat taccgccatg cattagttat taatagtaat caattacggg gtcattagtt
     4801 catagcccat atatggagtt ccg
//
