LOCUS       sequence-474428-       11804 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For overexpression of mCherry-P2AT2A-EGFP-BRD4-NUT-KS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11804)
  AUTHORS   Zhang Y, Stoppelkamp I, Fernandez-Pernas P, Allram M, Charman M, 
            Magalhaes AP, Piedavent-Salomon M, Sommer G, Sung YC, Meyer K, Grams
            N, Halko E, Dongre S, Meierhofer D, Malszycki M, Ilik IA, Aktas T, 
            Kraushar ML, Vastenhouw N, Weitzman MD, Grebien F, Niskanen H, Hnisz
            D
  TITLE     Probing condensate microenvironments with a micropeptide killswitch.
  JOURNAL   Nature. 2025 Jun 4. doi: 10.1038/s41586-025-09141-5.
  PUBMED    40468084
REFERENCE   2  (bases 1 to 11804)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2025 Jun 4. doi: 10.1038/s41586-025-09141-5."
COMMENT     Sequence Label: pRK5_mCherry-P2AT2A-EGFP-BRD4-NUT-KS
FEATURES             Location/Qualifiers
     source          1..11804
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        14..393
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        394..597
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     548..568
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     594..618
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        828..846
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     828..845
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     859..878
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      933..942
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1656..1712
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             1722..1775
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(1776..1778,1779..1781,1782..2492)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1776..1778,1779..1781,1782..2492)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1821..1842)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2082..2101)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2429..2450
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     regulatory      8104..8113
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    8117..8251
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8154..8173)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8208..8227
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        8320..8677
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        8320..8511
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(8343..8363)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      8528..8663
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8697..8714)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8697..8713)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8706..8728)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8926..9381
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9013..9032)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9223..9244
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9398..9417)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9517..9539
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9577..9595)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9663..9767
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9768..9836,9837..10628)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9986..10005)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10799..11387
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11288..11307
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11541..11558
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11675..11696
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11711..11716,11717..11734,11735..11741)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11749..11765
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11754..11776
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11773..11789
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11773..11789
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttcgagctcg cccgacattg attattgact agttattaat agtaatcaat tacggggtca
       61 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
      121 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
      181 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
      241 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
      301 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
      361 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
      421 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      481 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      541 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt
      601 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
      661 caccgggacc gatccagcct ccgcggccgg gaacggtgca ttggaacgcg gattccccgt
      721 gccaagagtg acgtaagtac cgcctataga gtctataggc ccaccccctt ggcttcgtta
      781 gaacgcggct acaattaata cataacctta tgtatcatac acatacgatt taggtgacac
      841 tatagaataa catccacttt gcctttctct ccacaggtgt ccactcccag gtccaactgc
      901 acctcggttc tatcgattga attcgtaccg gtgccaccat ggtttccaaa ggagaggagg
      961 ataatatggc tataattaaa gagtttatgc ggttcaaggt gcatatggag ggatctgtga
     1021 acggtcacga gttcgaaatc gagggcgaag gcgaggggcg accctatgaa gggacacaga
     1081 cggccaaact gaaggtgacc aagggtggcc ccctgccttt cgcctgggac atcctgtcac
     1141 cccagtttat gtacggctct aaggcttacg ttaagcaccc tgccgatatt ccggactact
     1201 tgaaactgag cttccctgaa gggttcaagt gggaacgggt aatgaatttt gaggatggcg
     1261 gtgtagtcac agttacccag gacagctctc ttcaggacgg agaatttatc tataaggtta
     1321 aacttcgggg cactaacttc ccatcagacg gccccgtgat gcagaagaaa actatgggct
     1381 gggaagccag ctcagaacgc atgtatcccg aggacggagc actgaaggga gaaatcaagc
     1441 agcgactcaa gctgaaggat gggggacatt atgatgctga ggtcaaaacc acctataagg
     1501 ccaagaaacc cgttcagctt cctggggcat acaacgtgaa tatcaaactg gatattacat
     1561 cccataacga agactatacc atcgtggaac agtacgagcg ggccgagggc agacatagca
     1621 cagggggcat ggatgaattg tacaagggaa gcggagctac taacttcagc ctgctgaagc
     1681 aggctggaga cgtggaggag aaccctggac ctggaagcgg agagggcaga ggaagtcttc
     1741 taacatgcgg tgacgtggag gagaatcccg gcccaatggt gagcaagggc gaggagctgt
     1801 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     1861 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
     1921 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg
     1981 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     2041 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     2101 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     2161 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
     2221 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc
     2281 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
     2341 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga
     2401 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     2461 ggatcactct cggcatggac gagctgtaca agatgtctgc ggagagcggc cctgggacga
     2521 gattgagaaa tctgccagta atgggggatg gactagaaac ttcccaaatg tctacaacac
     2581 aggcccaggc ccaaccccag ccagccaacg cagccagcac caaccccccg cccccagaga
     2641 cctccaaccc taacaagccc aagaggcaga ccaaccaact gcaatacctg ctcagagtgg
     2701 tgctcaagac actatggaaa caccagtttg catggccttt ccagcagcct gtggatgccg
     2761 tcaagctgaa cctccctgat tactataaga tcattaaaac gcctatggat atgggaacaa
     2821 taaagaagcg cttggaaaac aactattact ggaatgctca ggaatgtatc caggacttca
     2881 acactatgtt tacaaattgt tacatctaca acaagcctgg agatgacata gtcttaatgg
     2941 cagaagctct ggaaaagctc ttcttgcaaa aaataaatga gctacccaca gaagaaaccg
     3001 agatcatgat agtccaggca aaaggaagag gacgtgggag gaaagaaaca gggacagcaa
     3061 aacctggcgt ttccacggta ccaaacacaa ctcaagcatc gactcctccg cagacccaga
     3121 cccctcagcc gaatcctcct cctgtgcagg ccacgcctca ccccttccct gccgtcaccc
     3181 cggacctcat cgtccagacc cctgtcatga cagtggtgcc tccccagcca ctgcagacgc
     3241 ccccgccagt gcccccccag ccacaacccc cacccgctcc agctccccag cccgtacaga
     3301 gccacccacc catcatcgcg gccaccccac agcctgtgaa gacaaagaag ggagtgaaga
     3361 ggaaagcaga caccaccacc cccaccacca ttgaccccat tcacgagcca ccctcgctgc
     3421 ccccggagcc caagaccacc aagctgggcc agcggcggga gagcagccgg cctgtgaaac
     3481 ctccaaagaa ggacgtgccc gactctcagc agcacccagc accagagaag agcagcaagg
     3541 tctcggagca gctcaagtgc tgcagcggca tcctcaagga gatgtttgcc aagaagcacg
     3601 ccgcctacgc ctggcccttc tacaagcctg tggacgtgga ggcactgggc ctacacgact
     3661 actgtgacat catcaagcac cccatggaca tgagcacaat caagtctaaa ctggaggccc
     3721 gtgagtaccg tgatgctcag gagtttggtg ctgacgtccg attgatgttc tccaactgct
     3781 ataagtacaa ccctcctgac catgaggtgg tggccatggc ccgcaagctc caggatgtgt
     3841 tcgaaatgcg ctttgccaag atgccggacg agcctgagga gccagtggtg gccgtgtcct
     3901 ccccggcagt gccccctccc accaaggttg tggccccgcc ctcatccagc gacagcagca
     3961 gcgatagctc ctcggacagt gacagttcga ctgatgactc tgaggaggag cgagcccagc
     4021 ggctggctga gctccaggag cagctcaaag ccgtgcacga gcagcttgca gccctctctc
     4081 agccccagca gaacaaacca aagaaaaagg agaaagacaa gaaggaaaag aaaaaagaaa
     4141 agcacaaaag gaaagaggaa gtggaagaga ataaaaaaag caaagccaag gaacctcctc
     4201 ctaaaaagac gaagaaaaat aatagcagca acagcaatgt gagcaagaag gagccagcgc
     4261 ccatgaagag caagccccct cccacgtatg agtcggagga agaggacaag tgcaagccta
     4321 tgtcctatga ggagaagcgg cagctcagct tggacatcaa caagctcccc ggcgagaagc
     4381 tgggccgcgt ggtgcatatc atccagtcac gggagccctc cctgaagaat tccaaccccg
     4441 acgagattga aatcgacttt gagaccctga agccgtccac actgcgtgag ctggagcgct
     4501 atgtcacctc ctgtttgcgg aagaaaagga aacctcaagc tgagaaagtt gatgtgattg
     4561 ccggctcctc caagatgaag ggcttctcgt cctcagagtc ggagagctcc agtgagtcca
     4621 gctcctctga cagcgaagac tccgaaacag catctgcatt gccgggaccg gatatgagca
     4681 tgaaacctag tgccgccctg tctccatccc ctgcacttcc ctttctccca ccaacttctg
     4741 acccaccaga ccacccaccc agggagccac ctccacagcc catcatgcct tcagtattct
     4801 ctccagacaa ccctctgatg ctctctgctt tccccagctc actgttggtg acaggggacg
     4861 ggggcccttg cctcagtggg gctggggctg gcaaggtcat tgtcaaagtc aagacagaag
     4921 gggggtcagc tgagccctct caaactcaga actttatcct tactcagact gccctcaatt
     4981 cgactgcccc gggcactccc tgtggaggcc ttgagggtcc tgcacctcca tttgtgacag
     5041 catctaatgt gaagaccatt ctgccctcta aggctgttgg tgtcagccag gagggtcctc
     5101 caggccttcc gcctcagcct ccaccaccag ttgctcaact ggtccccatt gtgcccctgg
     5161 aaaaagcttg gccagggcca catgggacaa ccggggaagg aggtcctgtg gccactctat
     5221 ccaagccttc cctaggtgac cgctccaaaa tttccaagga cgtttatgag aacttccgtc
     5281 agtggcagcg ttacaaagcc ttggcccgga ggcacctatc ccagagtcct gacacagaag
     5341 ctctttcctg ttttcttatc ccagtgcttc gttccctggc ccggctgaag cccactatga
     5401 ccctggagga gggactgcca ttggctgtgc aggagtggga gcacaccagc aactttgacc
     5461 ggatgatctt ttatgagatg gcagaaaggt tcatggagtt tgaggctgag gagatgcaga
     5521 ttcagaacac acagctgatg aatgggtctc agggcctgtc tcctgcaacc cctttgaaac
     5581 ttgatcctct agggcccctg gcctctgagg tttgccagca gccagtgtac attccgaaga
     5641 aggcagcttc taaaacaaga gctcctcgcc ggcgtcagcg taaagcccag agacctcctg
     5701 ctcctgaggc acccaaggag atcccaccag aagctgtgaa ggagtatgtt gacatcatgg
     5761 aatggctggt ggggactcac ttggccactg gggagtcaga tggaaaacaa gaggaagaag
     5821 ggcagcagca ggaggaggaa gggatgtatc cagatccagg tctcctgagc tacatcaatg
     5881 agctgtgttc tcagaaggtc tttgtctcca aggtggaggc tgtcattcac cctcaatttc
     5941 tggcagatct gctgtcccca gaaaaacaga gagatccctt ggccttaatt gaggagctag
     6001 agcaagaaga aggactcact cttgcccagc tggtccagaa gcgactcatg gccttggaag
     6061 aggaggaaga tgcagaggcg cctccaagtt tcagtggcgc tcagttggac tcaagtcctt
     6121 ctggttctgt tgaggatgaa gatggggatg ggcggcttcg gccctcacct gggcttcagg
     6181 gggctggggg cgccgcttgc cttggaaagg tttcttcttc aggaaaacgg gcaagagaag
     6241 tgcatggtgg gcaggagcaa gccctagata gccccagagg gatgcacagg gatgggaaca
     6301 ctctgccatc ccccagcagc tgggacctgc agccagaact tgcagctcca cagggaactc
     6361 cgggaccctt gggtgtggag aggagagggt ctgggaaggt tataaaccag gtatctctac
     6421 atcaggatgg ccatctagga ggcgctgggc ctcctgggca ctgcctggtg gctgatagga
     6481 cttcagaggc tctgcccctt tgttggcagg gaggcttcca gcctgagagc actcccagtt
     6541 tggatgctgg acttgcagag ctggctcctc tgcaaggaca agggttagaa aagcaagtcc
     6601 tgggattgca gaaaggacaa caaacagggg gtcgtggagt gcttcctcaa gggaaggagc
     6661 ctttagcagt gccctgggaa ggctcttcag gagccatgtg gggagatgac agaggtaccc
     6721 ccatggctca gagttatgat cagaatcctt cccctagagc agctggggag agggacgatg
     6781 tctgtctcag cccaggagtt tggctgagca gtgagatgga tgctgtaggc ttggagctgc
     6841 ctgtacaaat agaggaggtc atagagagct tccaagttga gaagtgtgta actgagtatc
     6901 aggaaggctg ccagggactg ggctccaggg gcaacatttc cctgggtcct ggagaaaccc
     6961 tagtacctgg ggatacggag agcagtgtga ttccctgtgg aggcacagtt gcggcagctg
     7021 ccctagaaaa gagaaactat tgcagcttgc caggaccttt gagggccaac agcccaccct
     7081 tgaggtccaa agaaaatcaa gaacagagct gtgaaaccgt agggcatccc agtgatctgt
     7141 gggcagaagg ctgcttccca ttgctagaaa gtggtgattc cacactgggg tcttccaaag
     7201 aaacccttcc acccacatgc caaggcaatc tccttatcat ggggactgag gatgcctcct
     7261 ccttgcctga agccagtcaa gaggcaggga gcagaggcaa ttccttttct cctctgttgg
     7321 aaaccataga acctgtcaac atactagatg ttaaagatga ctgtggcctc caactaaggg
     7381 tcagcgagga cacctgccca ctgaatgttc attcttatga cccccaagga gaaggcaggg
     7441 tggatcctga tctgtccaag cctaaaaacc ttgctccttt acaagagagt caggagtctt
     7501 acacaactgg gactcccaaa gcaacatctt ctcaccaggg ccttggaagc actttgccta
     7561 gaaggggaac caggaatgcc atagttccga gagaaacttc tgttagtaaa acacacaggt
     7621 cagcagacag ggccaaagga aaggagaaaa agaaaaagga agcagaggaa gaggatgagg
     7681 aactctccaa ctttgcttac ctcttggcct ctaaacttag cctctcacca agggagcatc
     7741 ccctcagtcc tcaccatgcc tcaggaggtc agggcagcca gagagcatcc cacctgctcc
     7801 ctgctggagc aaaaggcccc agcaaacttc catatcctgt tgccaagtct gggaagcgag
     7861 ctctagctgg aggtccagcc cctactgaaa agacacccca ctcaggagct caacttgggg
     7921 tccccaggga gaaaccccta gctctgggag tagttcgacc ctcacagcct cgtaaaaggc
     7981 ggtgtgacag ttttgtcacg ggcagaagga agaaacgacg tcgtagccag atgatgatga
     8041 tgaataagtt ggttctagcg cagttcttct tctcctgtct atgagtcgac ctgcagaagc
     8101 ttggccgcca tggcccaact tgtttattgc agcttataat ggttacaaat aaagcaatag
     8161 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     8221 actcatcaat gtatcttatc atgtctggat cgggaattaa ttcggcgcag caccatggcc
     8281 tgaaataacc tctgaaagag gaacttggtt aggtaccttc tgaggcggaa agaaccagct
     8341 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     8401 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     8461 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     8521 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     8581 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     8641 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctgttaacag cttggcactg
     8701 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     8761 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     8821 tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt tctccttacg
     8881 catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg
     8941 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     9001 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     9061 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     9121 tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga
     9181 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     9241 ctggaacaac actcaaccct atctcgggct attcttttga tttataaggg attttgccga
     9301 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     9361 aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat
     9421 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     9481 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     9541 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     9601 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     9661 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     9721 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     9781 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     9841 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     9901 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     9961 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
    10021 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
    10081 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
    10141 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
    10201 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
    10261 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
    10321 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
    10381 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
    10441 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
    10501 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
    10561 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
    10621 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
    10681 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
    10741 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
    10801 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
    10861 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
    10921 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
    10981 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
    11041 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
    11101 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
    11161 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
    11221 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
    11281 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
    11341 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
    11401 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
    11461 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
    11521 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
    11581 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
    11641 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
    11701 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
    11761 aacaatttca cacaggaaac agctatgaca tgattacgaa ttaa
//
