LOCUS       sequence-474459-       10531 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpression KLF5 ORF from a EF1a promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10531)
  AUTHORS   Southard KM, Ardy RC, Tang A, O'Sullivan DD, Metzner E, 
            Guruvayurappan K, Norman TM
  TITLE     Comprehensive transcription factor perturbations recapitulate 
            fibroblast transcriptional states.
  JOURNAL   Nat Genet. 2025 Aug 6. doi: 10.1038/s41588-025-02284-1.
  PUBMED    40770575
REFERENCE   2  (bases 1 to 10531)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Genet. 
            2025 Aug 6. doi: 10.1038/s41588-025-02284-1."
COMMENT     Sequence Label: pRCA879 - KLF5 isoform 1 Puro-T2A-GFP (pRCA845...
FEATURES             Location/Qualifiers
     source          1..10531
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2616
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             2629..2682
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      2689..2698
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2695..2697,2698..2700,2701..3414)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2695..2697,2698..2700,2701..3411)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2740..2761)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3001..3020)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3348..3369
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    3457..3574
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3712..4890
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3943..4881
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4838..4858
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4901..4910
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    6388..6976
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6441..6461)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6859..6870)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7048..7281
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7359..7480
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7396..7415)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7450..7469
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7520..7655
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7675..7694)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7676..7694)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7704..7721)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7704..7720)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7713..7735)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7862..8317
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7949..7968)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8159..8180
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8343..8447
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8448..8516,8517..9308)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8666..8685)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9479..10067
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9968..9987
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10221..10238
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10355..10376
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10391..10396,10397..10414,10415..10421)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10429..10445
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10434..10456
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     10453..10469
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10453..10469
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     10488..10508
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        10490..10508
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtg gatcgggaga gggcagagga
     2641 agtctgctaa catgcggtga cgtcgaggag aatcctggcc caccggtcgc caccatggtg
     2701 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     2761 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     2821 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     2881 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     2941 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     3001 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     3061 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     3121 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     3181 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     3241 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     3301 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     3361 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtaacgaatt
     3421 ctagatcttg agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg
     3481 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
     3541 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
     3601 tccactttgg cgccggctcg aggcctgcag gtgcaaagat ggataaagtt ttaaacagag
     3661 aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat tggctccggt
     3721 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc
     3781 ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg
     3841 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc
     3901 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt
     3961 tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc
     4021 tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg
     4081 aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc
     4141 tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag
     4201 tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt
     4261 cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc
     4321 gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca
     4381 ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg
     4441 ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga
     4501 gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc
     4561 tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc
     4621 gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg
     4681 agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc
     4741 cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg
     4801 gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa
     4861 agtttttttc ttccatttca ggtgtcgtga ggctagcacc gccaccatgg ctacaagggt
     4921 gctgagcatg agcgcccgcc tgggacccgt gccccagccg ccggcgccgc aggacgagcc
     4981 ggtgttcgcg cagctcaagc cggtgctggg cgccgcgaat ccggcccgcg acgcggcgct
     5041 cttccccggc gaggagctga agcacgcgca ccaccgcccg caggcgcagc ccgcgcccgc
     5101 gcaggccccg cagccggccc agccgcccgc caccggcccg cggctgcctc cagaggacct
     5161 ggtccagaca agatgtgaaa tggagaagta tctgacacct cagcttcctc cagttcctat
     5221 aattccagag cataaaaagt atagacgaga cagtgcctca gtcgtagacc agttcttcac
     5281 tgacactgaa gggttacctt acagtatcaa catgaacgtc ttcctccctg acatcactca
     5341 cctgagaact ggcctctaca aatcccagag accgtgcgta acacacatca agacagaacc
     5401 tgttgccatt ttcagccacc agagtgaaac gactgcccct cctccggccc cgacccaggc
     5461 cctccctgag ttcaccagta tattcagctc acaccagacc gcagctccag aggtgaacaa
     5521 tattttcatc aaacaagaac ttcctacacc agatcttcat ctttctgtcc ctacccagca
     5581 gggccacctg taccagctac tgaatacacc ggatctagat atgcccagtt ctacaaatca
     5641 gacagcagca atggacactc ttaatgtttc tatgtcagct gccatggcag gccttaacac
     5701 acacacctct gctgttccgc agactgcagt gaaacaattc cagggcatgc ccccttgcac
     5761 atacacaatg ccaagtcagt ttcttccaca acaggccact tactttcccc cgtcaccacc
     5821 aagctcagag cctggaagtc cagatagaca agcagagatg ctccagaatt taaccccacc
     5881 tccatcctat gctgctacaa ttgcttctaa actggcaatt cacaatccaa atttacccac
     5941 caccctgcca gttaactcac aaaacatcca acctgtcaga tacaatagaa ggagtaaccc
     6001 cgatttggag aaacgacgca tccactactg cgattaccct ggttgcacaa aagtttatac
     6061 caagtcttct catttaaaag ctcacctgag gactcacact ggtgaaaagc catacaagtg
     6121 tacctgggaa ggctgcgact ggaggttcgc gcgatcggat gagctgaccc gccactaccg
     6181 gaagcacaca ggcgccaagc ccttccagtg cggggtgtgc aaccgcagct tctcgcgctc
     6241 tgaccacctg gccctgcata tgaagaggca ccagaactag taatgaacta gtccgtaact
     6301 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgagg gttatccgct
     6361 cgtcggaagt taacgcgtta agtcgacaat caacctctgg attacaaaat ttgtgaaaga
     6421 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     6481 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     6541 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     6601 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     6661 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     6721 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     6781 aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     6841 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     6901 ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt
     6961 tgggccgcct ccccgcgtcg actttaagac caatgactta caaggcagct gtagatctta
     7021 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag
     7081 atctgctttt tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct
     7141 ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag
     7201 tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt
     7261 cagtgtggaa aatctctagc agtacgtata gtagttcatg tcatcttatt attcagtatt
     7321 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
     7381 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     7441 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
     7501 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     7561 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     7621 attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat
     7681 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     7741 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     7801 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     7861 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     7921 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     7981 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     8041 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     8101 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     8161 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     8221 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     8281 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
     8341 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     8401 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     8461 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     8521 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     8581 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     8641 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     8701 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     8761 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     8821 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     8881 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     8941 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     9001 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     9061 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     9121 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     9181 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     9241 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     9301 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     9361 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     9421 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     9481 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     9541 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     9601 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     9661 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     9721 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     9781 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     9841 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     9901 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     9961 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
    10021 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
    10081 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
    10141 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
    10201 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
    10261 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
    10321 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
    10381 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
    10441 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
    10501 actaaaggga acaaaagctg gagctgcaag c
//
