LOCUS       sequence-474679-       12446 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express LgBiT-NCOR2 Fusion Protein in Mammalian Cells under a CMV 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12446)
  AUTHORS   Promega
  TITLE     Promega Plasmid Collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12446)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: LgBiT-NCOR2 Vector
FEATURES             Location/Qualifiers
     source          1..12446
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(4599..4617)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4600..4617)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     enhancer        4737..5040
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     5195..5215
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     promoter        5248..5295
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             5327..6121
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5381..5400)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5991..6010
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     rep_origin      6338..6883
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     6784..6803
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7000..7017
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    7167..7215
                     /note="synthetic polyadenylation signal"
     CDS             complement(7239..8276)
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     EVGRV"
     RBS             7252..7260
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     promoter        complement(8307..8664)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8321..8456
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        8473..8664
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     8621..8641
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     polyA_signal    8736..8817
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     8761..8780
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8994..9012
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 gatggcacgg cccatgagac cttcgatgct ggctgaggag atggctctgc ccacgcggcc
       61 ctccatcatg tcataggtgc gcttgggggc ggccgtctca tgggggggtc ctgagctgct
      121 tctgccgtcc tccttggagc actgggtcac agacatgcca ccctcatagg acaagacgtg
      181 gcccttcttg ccttcgtaga tgacgtggcc cttgggcagg ctgtcctccc ggccgcggtc
      241 caagcgactc gggctgtcct cgccgatgat cctggtgatg gtgcccttgt acaggacgtc
      301 agctggcgtg ccgtgggtga tggagccgcg gtatgtgatg gcgctgtccg agggcacccg
      361 tgtgctggga atgcctttgg tgatgcttcc gcccggaact gagcccagag ctgtccctct
      421 cagcacggac gcctcctggg ctgtgggcac ccccaggctc tccggtggcc cagcctggcc
      481 ccgtggggac agctgctcct gcttcactcc gctgaagggt gccagctttt tggggtccat
      541 gggcaggggc agccccatgg tgacagggcc caccggggcc ttggcatgct ctgagtacgg
      601 gacgtggagc tggaccgaca ttccttggga gatggcacct atttgcctct cgaggacgct
      661 ggggtgcttg gcagaggaga tgaggggagg cgggttggag atggtgggtg ggcgcggcag
      721 gacgggccgg gcagtgtcat ggaggcccag gggcagtggg tgaccaggtg gagcgtagga
      781 gaaggctgag gggtccgggg catgcgggga ggccttgatc acctcacggg ggggcacggg
      841 gaagggcagg ccggaagtcc agcaaggggg gtccccaggc agcttctggg cctcggctgc
      901 gaaggctggg aagaacacag gcttctctgc ctccttgtcg gcggggggtg ccgggctcct
      961 gctcttgccc cgggggctgc tgccaggctg ctgaggggcg tcgctctccg gctgcaggtt
     1021 ttgcggtggc ggtggggctg ggggagctgg cttggtggga gctgcgtcct cccggggggg
     1081 ctcatggact ttggtgacct ggatgggggg gatggcagcc gctcgctgct tcagctgctt
     1141 caggtccagt ggcttctggg gtgaggcatt ggcccggggg tcgccagtcg gggtgaggag
     1201 gctgggcctt ggggacagca gccggttctt gtcgccgccc tcggcctcat ccacctcgtc
     1261 tgcactgcag gtggcactgg agtcgctgtc ctggggggcg cccgagctct tggctgtggt
     1321 ggccctgccg ctcccgccct ccttcttctc tgccttgagc gccccctcgg ccgtggcctc
     1381 agcggcctcc gcgtccttgc ccttggccgg cccctcctcg gcttcctccg tgcactcgct
     1441 cttgacgggc tcctcggcct tccctgtgtc cactgccagc tcctcagccg cggggggctt
     1501 ctgctcctcc ccctcctcca ctgggggcgc tgctgcggtc tcctcctcct tctcctcctt
     1561 ggggaccaca ggaggaggtg cagagggcga tgggggtgct ggtgggggcg taggggctcc
     1621 ggtggcttca gaggccgggg tgggctcagt gggggccggg atgtcctccg gtggtggggt
     1681 gggtggctct gggggtggcc cgtcggcgcc cagggtggct gggggcttgg gcccattctg
     1741 ccctgtgtcc ttggcggcct cagtgtgagg agaggggatg ctctcggtgt ctgagctgtt
     1801 gttgacagtg gctgggccac tgcattcccc tctgggcacc tcattcccag aggcatgtaa
     1861 ggcttcagcc tcctccacca tctcctcctc atttccgctc acgcccgacg cctccatctc
     1921 ctcatcctcc accacgggcg ggaatgcagc ctcctcgctg gccgccgccg gcgctttctt
     1981 cttcttcctc cgcgcgttcc tctccttctc catcttcagc ttgtgctgct gcaagatctc
     2041 atcgaggttc tgcctcttct tgtagttgaa gtagaagttc ttacactgcg acacagtctt
     2101 ggagcccacc atccgggcga tggccgacca gttgcggccg tgttccagga gacctttctt
     2161 ggctgtttcc atttcttctt ctgtccagcg agaactctca ttcagctcca tggaggccag
     2221 ctcggcgctc tgctgggggg tgatggcctc ctcgctgttg gcctcattag ccattgagcg
     2281 ggtgatgcgg cctttgcgtc ttccctggct gttggcagtt ttgcggcctt tggaggccac
     2341 agcctccttc tcgtcgttgt cctcccctga ggtgtcgtct gtcttctcct tgaggaggtc
     2401 ttccttgtcg ttctccacct ccggcttctc ctcctccttc tccgcctcct tttccttctc
     2461 cttctcatct ttctcctcct ggctgctgcg gggcatgggc tgctgctgct gctgctgctg
     2521 ctgctgctgc tgctgttgtt gctgctgctg gctcttgccg cggcgccgat agctccgtct
     2581 caccaggctc ttatagttct cattcttctt agtcaggtag taatagagga cgcactcagc
     2641 cactgtcttc ctctccagga atgatgcgat caggccaaag ttcttgggat gctgcatgaa
     2701 cttctcccgg aaggtctcct tctcctgctc actccacatg ttcatgacct ggcggtcttt
     2761 gtacaccttc atggggtcgg ccataagccc gttcatgttg atgaacttga tgcgctgctg
     2821 gtcagcgtcg tacagcatgg gcgggatcac ggccagctgg cgcatctgct tctccaggtt
     2881 ctcctgctct gagaggccat cgatgatctc tgacacctcg tgctcgctgc gggcggccga
     2941 catggacagc ccactgcccc gctggcccac cctgctctgc atgcgctcct gcagctcgcg
     3001 ctgcttgcgg atctcaggga actgcttctc gtagtactcg cgcaccttgc tctccttggc
     3061 ccgccgccgg gggttgttct cgatgcgctc caccttcttc tcccaggcct ccatgagctg
     3121 gtcatagcgc tggcagaact tctgctccca ttgtttccga gcgtgattcc tcctcttgaa
     3181 gtacaagatt agcttcttcc gcatcgcctg gtttattttg atgttctcat gatactgccg
     3241 ggtgtcggag ggctggttgt acagcggcag ctccacctgg ggccccaggc cttccagaat
     3301 ccgatgtgca gcttcagcct tcttccggtt ctcgtcgtag atgatctgca ccaggctgcg
     3361 gtgcttcgac tcgatgggcg gcggtgacac gggcttctca ggctcgggcg gcttggcagc
     3421 ctcctcctcc agctgttgct gcttcttctt cagcttagag atctgctgct ctaccatggt
     3481 gatctctcgg tccacgcggt ccatgttctg gatcagctcc tccttggaca gccgtggcgg
     3541 caccagctcc agctcagggt cagtgtgcgg ggggctgggg ggagacaccg gttccagctt
     3601 gcccgtcagg ctacggtcct tggtgaggtc ttcagatccc gcaggctggc ccgtggccag
     3661 caggggtgac ggtcgcagca gggggtcagg cagcagctct agccgagggc gcttgctttc
     3721 aatgaactcc atctctgact tccccagctc gggcaggtat gagtgggact ctggccgcag
     3781 gtggagctcc tgggaccgtt cattcccggg ctggaactca gacagcaggg agggcctccg
     3841 ccgctggggc tggatgatgg agccgggcga caggtgggag gcatagtcgc gggagtggtg
     3901 ctggtactcc aggagcccga cgtccgtgtg cgtccgggcg atctgcactg ggtaggaaag
     3961 gctgtggggc gggtagcggg gctcagtggc cctccacgtc tgtgccacag gctgtgtgga
     4021 tcccgacatg gcgatcgcac cgctcgagcc tccacctccg ctcccgccac caccggagct
     4081 cccactgttg atggttactc ggaacagcat ggagccgtcg ggggtgatca ggcgctcgtc
     4141 gataattttg ttgccgttcc acagggtccc tgttacagtg atctttttgc cgtcgaacac
     4201 ggcgatgcct tcatacggcc gtccgaaata gttcagcatg ttcggcgtaa ccccgtcgat
     4261 taccagtgtg ccatagggca ggatcacctt aaagtgatga tcatccacag ggtacaccac
     4321 cttaaacacc tcttcgatct gggccatttg gtcggcgctc agaccttcat acgggatgat
     4381 gacatggatg tcgatcttca gggcattttc accgctccgg acaatccttt ggatcggagt
     4441 tacggacacg gcgagattct gcagcaaact ggacacacct ccctgttcaa ggacttggtc
     4501 caggttgtag gcggctgtct gttcccagtc cccaacgaaa tcttcgagtg tgaagaccat
     4561 ggtgagcgct agctgcaaaa agaacaagta gcttgtattc tatagtgtca cctaaatggg
     4621 cgatctgacg gttcactaaa ccagctctgc ttatataggc ctccctggcg cgccactagt
     4681 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
     4741 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
     4801 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
     4861 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
     4921 ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
     4981 accttacggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
     5041 gtgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt
     5101 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
     5161 tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg
     5221 tgggaggtca aatcgaattc taatactgtt gacaattaat catcggcata gtatatcggc
     5281 atagtataat acgacaaggt gaggaactaa acccaggagg cagatcatga ttgaacaaga
     5341 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     5401 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     5461 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc
     5521 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     5581 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     5641 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     5701 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     5761 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     5821 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt
     5881 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     5941 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     6001 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     6061 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     6121 attcgaaatg accgaccaag cgacgcccaa ccggtatcag ctcactcaaa ggcggtaata
     6181 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtcaaacat gagaattaca
     6241 acttatatcg tatggggctg acttcaggtg ctacatttga agagataaat tgcactgaaa
     6301 tctagaaata ttttatctga ttaataagat gatcttcttg agatcgtttt ggtctgcgcg
     6361 taatctcttg ctctgaaaac gaaaaaaccg ccttgcaggg cggtttttcg aaggttctct
     6421 gagctaccaa ctctttgaac cgaggtaact ggcttggagg agcgcagtca ccaaaacttg
     6481 tcctttcagt ttagccttaa ccggcgcatg acttcaagac taactcctct aaatcaatta
     6541 ccagtggctg ctgccagtgg tgcttttgca tgtctttccg ggttggactc aagacgatag
     6601 ttaccggata aggcgcagcg gtcggactga acggggggtt cgtgcataca gtccagcttg
     6661 gagcgaactg cctacccgga actgagtgtc aggcgtggaa tgagacaaac gcggccataa
     6721 cagcggaatg acaccggtaa accgaaaggc aggaacagga gagcgcacga gggagccgcc
     6781 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccaccact gatttgagcg
     6841 tcagatttcg tgatgcttgt caggggggcg gagcctatgg aaaaacggct ttgccgcggc
     6901 cctctcactt ccctgttaag tatcttcctg gcatcttcca ggaaatctcc gccccgttcg
     6961 taagccattt ccgctcgccg cagtcgaacg accgagcgta gcgagtcagt gagcgaggaa
     7021 gcggaatata tcctgtatca catattctgc tgacgcaccg gtgcagcctt ttttctcctg
     7081 ccacatgaag cacttcactg acaccctcat cagtgccaac atagtaagcc agtatacact
     7141 ccgctagcgc tgatgtccgg aatcgtcaca caaaaaacca acacacagat gtaatgaaaa
     7201 taaagatatt ttattgcggc catcgtgatg gctagagtct aaactcgacc tacctcctta
     7261 gcgcgcggtc gtgtactggg ccggcggttg ccgctgtcgg ccagcacctc gacgcagccg
     7321 tcggtccata cggctgcgct ccggcgagcg atttgtgtgc gaccgacggt gccggccccg
     7381 ctgcggacga tggcatcgca gcggccttga gcccaggcag catcgtcgaa gttgccgtcc
     7441 acgaggctct ggtagagctg atccaggccg atgcgcagca tgtaggctcg cagacgaggg
     7501 ctgccggcca gctcgggatg ccggcgctcg aagtagcgag tctgctgctc catgcaagcc
     7561 agccagggcc gccagaagaa gatgttggcc acctcgtact gactgtcccc gaacatagct
     7621 tcggaccagt cgattacggc ggtgatgcgg ccgttgtcgg tcaggacgtt gttgctgccg
     7681 aagtcggcat ggacgaggtg gcgcacctcg ggacagtctt cggcccacag catcagttcg
     7741 tccagggctt gagctacgct ggcggacacg gtgtcgtcca tcacggtctg ccagtggtag
     7801 acatggggat cagcaatggc gcaaatgaaa tcccgccagg ttgtgtactg gccgatgcct
     7861 tggggcccga atgggccgaa gccgctggtt tgactgaggt cggcggcggc aatagcatcc
     7921 atagcttcgg cgacaggctg taacacagca ggcagctctg tttcggggag gtcttggaga
     7981 gtaacgcctt gggcgcgtct actgatgcag tatgtcaggc tctcgctgaa ctcgccgatg
     8041 tccaacactt cggggatggg tagtgcagcg ctggcgaagt ggcggtacac gtagcggtct
     8101 ttgtagaagc catcagcgca gctattcacc cgcagtacat agccgcgtcc gccgacatcg
     8161 aagctgaagg ctcggctctc ttcgccctcc gacaactgca tcaggtcgct cacactgtcg
     8221 aacttctcga tgagaaattt ttcaacgctg gtagcggtga gttcgggctt cttcatggtg
     8281 gcgctagtgt cagaagaatc gagctttttg caaaagccta ggcctccaaa aaagcctcct
     8341 cactacttct ggaatagctc agaggccgag gcggcctcgg cctctgcata aataaaaaaa
     8401 attagtcagc catggggcgg agaatgggcg gaactgggcg gagttagggg cgggatgggc
     8461 ggagttaggg gcgggactat ggttgctgac taattgagat gcatgctttg catacttctg
     8521 cctgctgggg agcctgggga ctttccacac ctggttgctg actaattgag atgcatgctt
     8581 tgcatacttc tgcctgctgg ggagcctggg gactttccac accctaactg acacacattc
     8641 cacagctggt tctttccgcc tcagaaggta cctaaccaag ttcctctttc agaggttatt
     8701 tcaggccatg gtgctgcgca agacgcgtga cgtcggcagt gaaaaaaatg ctttatttgt
     8761 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     8821 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
     8881 agcaagtaaa acctctacaa atgtggtatg gctgattatg atccgcggcc gcaatacgtc
     8941 gacgttatca gctgacttcg tacgagagcc taggtgtttc cccaaggggt tatgctagtt
     9001 attgctcagc ggtggcagca gccaactcag cttcctttcg ggctttgtta gcagccggat
     9061 cagcttgcat gcctgcaggt cgactctaga ggatccccgg gtaccgagcc cgaattcgtt
     9121 taaacctcgc tgtcggagag tgtctcgtac tgcgagcaga gcagtggctt gggctcctcg
     9181 tcccaggcgt ggtgggggcc agcgaggggc ccgctgcccg cggggaggcc cggtgggggt
     9241 ggggaagcca tgacacccgc ctgcagccgc atgatcaggg ggttgtaggg gaatggcgtg
     9301 gaacctgcgg acgagggcct gtcctcccac acgcggttgg tgagcggcgt ccggcggttg
     9361 cagtctccct ccgagtgcac tgaggagaca gagggtggcc ggtccccaga tgccaggccc
     9421 ggggccgggg acttggcttt tcggctgctg ggtctgccag agaccttggc cttcccgccg
     9481 ccacctgttc cggtgatggc gggcatattg aagatctccg tcccaggctg gctgatattg
     9541 tattcaggct cattccggtt gtgggtgttc agcttcttgt tgatctcttg cttcttggac
     9601 ttgaccatgg cggagttgct ctcggtcagc ttgctgaaga aggctggcgg ctggctggtg
     9661 ttgcctggag acttggagcc catcctgctg ggctccgtct gttccccatc ccggtacagc
     9721 agcgggtaca cagcactccg ggagtgccct ggctccgtca tgccctccgg tggggacaca
     9781 ggttcaatac cgtcctcacc accacccaag accgacgtct tgtttggctc tggagacctc
     9841 ttgccccctt cgctgtgggg ggagccacgg gccggggcac catggtccgg gggcgggagg
     9901 tagaggtcac tgggtgggcg gcggaggtcc aggacggggc agctggcccc agggaaggag
     9961 tagagggggg cgggcagggg tgcgctgagc tgctgtgggt ggtgccgggt gtagtcctgt
    10021 gtgatgacct cactgatgtg ctgggccagg gtgaccaccc gctggtgacc tttgacccct
    10081 ggggcggtct ggagcagcgg gctggacgag ggctggctct caggcagcgg ccgcaggtgt
    10141 gggaggtggg cggcctcccc gccaagcttc acggggcctg gctgcttggg ccgcagctcc
    10201 ccctccaggt ggctcttgtc gagctcttcc aggtgcttgg ggagcccctt gtcgtgggtc
    10261 agactgggtg agctcacagg gctgacgggc tccacccctt cggggctgta gctgctgccg
    10321 tggtaaccca gagaacggag ttccagttcc tggatggaaa agggtttact ttgagtcttt
    10381 tcccggtgcg ggtccgaggc cgaggcaggt ggcgccggcg ggtccgggct ggcgtggtga
    10441 ggtgcgaggt tcttcgcagg ggtgcgggcg atggtggcgt ggccagagac aggaggcact
    10501 aggggccggg gctccgagcc cttgctgggg gaggaggcgg gctccagccc ggagcgggct
    10561 gggggcttgg cgaggaaggc atggccggtg tctgctcggg gccgctctgg ccgggcgacc
    10621 cggggggcct ccttgggcag caagacgggc tccatgaggg tagggtagac cccatcgagg
    10681 gtgccgccca gtgggcagtg ggtggcaggt gggaatgtgg cagccgggcg aacgggtgag
    10741 gaggtggagg tggacctcag gaccgtgggc gtgctgggct ccacagcggt gatgataccc
    10801 ttcatgcctg tgttgtgaag cacactgggt ctctgctgga gggcatcctg ggtccgaggg
    10861 gagatgggcg agtgctggtg ggcatgggag tgggaggcgg ggcggctgct gctgccccca
    10921 cccccgccgc tgctgccgct gctgccgctg ctctgctctg taccaggtct ccagatgggt
    10981 gcgtgctcca ccgtcgtggt ggacgtgagg atggactttt cccgctcccg atcccggtcc
    11041 cgctctcgat cccggtctcg ctcccgctcg gacgaggacg tggtggttgg ttttgtcaag
    11101 tgtgttggac ctcctgggga gagtggggag ctgctgtggc ggctgctgaa gggctggggc
    11161 gcggtgggga ggtaggcaag gcggtccatg gcggtggctg gggtgcctgg tgtcgggggc
    11221 acgagcacag gcaggtgtgg cacttgggac aggtcgatga tgcctcgggg acccgcagcg
    11281 tagttgagtg ccagcgagga ctcgcggggc gagaggcccc tcagcatatc agctcgctgg
    11341 gccatggcgg tggccgcgtt gtggtgcatc tgctgcgagg tgatgtagtc attgatgatg
    11401 gtctgccggt tctccagcgc cgccgtgtcg gggtagccgc ggatgaggta gggtgggtac
    11461 aggtgcgggt aggtggggtt gggggccagg tgtcggggca ggtagtaggc agcggctgcg
    11521 tccagaggga tgccgcgggg tatggaggtg gggtcgaagg ccagggggat gtggctgcga
    11581 tacaggtcca cgccactcac gccccgaagc aggtgctcat agggcgagat ggggtgtggg
    11641 tggtgctcgg gcacggtgct gtgcggggac ttggcgatct cacgaggcgt cgacgtcagc
    11701 tttcggtcct gggatgcctt gctggacgaa aggctgccct cctgcaggcg cggcgtgggc
    11761 tcccgcgtgg tcacgggcga acctcgtggg aggtggccgg caaagggtgc cccgtggtcc
    11821 tcataggtta gggggctctg ccgcggcttg cccagctcag gcacaatgac cggggcgccg
    11881 cgcgcaatgg agccccccga gctgctggcg gtccctggcc ggctcttcag gctctcctcg
    11941 tagcaggcac gttccagtgc ccgggcgtcg gccatcacat ccagcgggtg cacgggtggg
    12001 aacgtccggc cggggctgcc gatgagggag cgtacgtcgt gctttttgga gccagtggtg
    12061 gacgcgccgg tgtcgtactt gagcggggtg ccctgcgtga tggagccctc cttgagcggc
    12121 cgcggggcca ggggcagctc gggcgtgtgc cgcagctcct cgcgcgggat ctcatggatg
    12181 gagcggcccg cctccttcac cgtggccacc aggccctcat gggccggctt cagcttcagg
    12241 gggcccaggg cctgcgtctt gtaggcctcg gtcaggtccc gtgagggcgg tgggggcgga
    12301 ggcgtgccct cccgctttag gagcttggcc tcccgacgca ggtagtcctc ctgtgcctcc
    12361 acgtaggacc gagggatccc ttgtgtgatg gacccgcgga tgtggtgctg ctctttgagg
    12421 tggtgggggc tgtgtcgctc cggcgg
//
