LOCUS       sequence-475539-       23842 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T-DNA vector for dSpCas9 mediated gene activation for monocot 
            plants; NGG PAM; dSpCas9-Act3.0 was driven by 2x35s and the sgRNA 
            was driven by OsU3 promoter; Hygromycin for plants selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 23842)
  AUTHORS   Cheng Y, Li G, Qi A, Mandlik R, Pan C, Wang D, Ge S, Qi Y
  TITLE     A comprehensive all-in-one CRISPR toolbox for large-scale screens in
            plants.
  JOURNAL   Plant Cell. 2025 Apr 9:koaf081. doi: 10.1093/plcell/koaf081.
  PUBMED    40202185
REFERENCE   2  (bases 1 to 23842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2025 Apr 9:koaf081. doi: 10.1093/plcell/koaf081."
COMMENT     Sequence Label: pYPQAT60
FEATURES             Location/Qualifiers
     source          1..23842
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..26)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    34..50
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(58..64,65..82,83..88))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    103..124
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        315..991
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             1058..2083
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    2124..2298
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    2376..2400
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             4024..4818
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      4905..5493
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5394..5413
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5647..5664
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    5679..5819
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(5745..5764)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5905..5927
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      6163..6357
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(6423..7496)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(7925..8554)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    9849..9873
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     10076..10092
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        10513..10857
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    10917..10941
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             10963..11028
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             11035..11055
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(11080..11103,11104..11106,11107..13593,13594..13596,
                     13597..15180)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     RBS             15093..15101
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             15181..15228
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             15406..15459
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             15463..15849
                     /codon_start=1
                     /product="bacteriophage MS2 coat protein"
                     /label=MS2-N55K
                     /note="binds with high affinity to a specific stem-loop 
                     structure in the viral RNA (Lim et al., 1994)"
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
                     CSVRQSSAQKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELI
                     VKAMQGLLKDGNPIPSAIAANSGIY"
     CDS             15850..15870
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             15913..15939
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             15919..15945
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             15958..15978
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             15985..16005
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             16069..16125
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             16141..16197
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             16213..16269
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             16285..16341
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             16357..16413
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             16429..16485
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             16501..16557
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             16573..16629
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             16645..16701
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             16717..16773
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             16855..16875
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        17515..19512
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             20260..20286
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             20377..21087
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             21472..21633
                     /codon_start=1
                     /product="B1 domain of Streptococcal protein G   "
                     /label=GB1
                     /note="effective as a solubilizing fusion partner (Cheng 
                     and Patel, 2004)"
                     /translation="YKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDD
                     ATKTFTVTE"
     CDS             21661..21711
                     /codon_start=1
                     /product="nuclear localization signal from the human T-cell
                     leukemia virus type 1 (HTLV-1) Rex protein"
                     /label=Rex NLS
                     /translation="PKTRRRPRRSQRKRPPT"
     protein_bind    22382..22402
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        22439..22816
                     /label=OsU3 promoter
                     /note="Oryza sativa (rice) snRNA U3 promoter"
     CDS             complement(22787..23119)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGAGLCHG
                     SSAQLF"
     primer_bind     22984..23000
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     23010..23029
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        23010..23028
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(23076..23096)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(23076..23094)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(23115..23131)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    23139..23155
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(23163..23169,23170..23187,23188..23193))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    23208..23229
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     misc_RNA        23288..23306
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     misc_RNA        23358..23376
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     protein_bind    complement(23495..23519)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     terminator      23583..23835
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
ORIGIN
        1 cgtaatcatg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
       61 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
      121 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
      181 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attggctaga gcagcttgcc
      241 aacatggtgg agcacgacac tctcgtctac tccaagaata tcaaagatac agtctcagaa
      301 gaccaaaggg ctattgagac ttttcaacaa agggtaatat cgggaaacct cctcggattc
      361 cattgcccag ctatctgtca cttcatcaaa aggacagtag aaaaggaagg tggcacctac
      421 aaatgccatc attgcgataa aggaaaggct atcgttcaag atgcctctgc cgacagtggt
      481 cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt tccaaccacg
      541 tcttcaaagc aagtggattg atgtgaacat ggtggagcac gacactctcg tctactccaa
      601 gaatatcaaa gatacagtct cagaagacca aagggctatt gagacttttc aacaaagggt
      661 aatatcggga aacctcctcg gattccattg cccagctatc tgtcacttca tcaaaaggac
      721 agtagaaaag gaaggtggca cctacaaatg ccatcattgc gataaaggaa aggctatcgt
      781 tcaagatgcc tctgccgaca gtggtcccaa agatggaccc ccacccacga ggagcatcgt
      841 ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg atatctccac
      901 tgacgtaagg gatgacgcac aatcccacta tccttcgcaa gacccttcct ctatataagg
      961 aagttcattt catttggaga ggacacgctg aaatcaccag tctctctcta caaatctatc
     1021 tctctcgagc tttcgcagat ccggggggca atgagatatg aaaaagcctg aactcaccgc
     1081 gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc gtctccgacc tgatgcagct
     1141 ctcggagggc gaagaatctc gtgctttcag cttcgatgta ggagggcgtg gatatgtcct
     1201 gcgggtaaat agctgcgccg atggtttcta caaagatcgt tatgtttatc ggcactttgc
     1261 atcggccgcg ctcccgattc cggaagtgct tgacattggg gagtttagcg agagcctgac
     1321 ctattgcatc tcccgccgtt cacagggtgt cacgttgcaa gacctgcctg aaaccgaact
     1381 gcccgctgtt ctacaaccgg tcgcggaggc tatggatgcg atcgctgcgg ccgatcttag
     1441 ccagacgagc gggttcggcc cattcggacc gcaaggaatc ggtcaataca ctacatggcg
     1501 tgatttcata tgcgcgattg ctgatcccca tgtgtatcac tggcaaactg tgatggacga
     1561 caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg atgctttggg ccgaggactg
     1621 ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc aacaatgtcc tgacggacaa
     1681 tggccgcata acagcggtca ttgactggag cgaggcgatg ttcggggatt cccaatacga
     1741 ggtcgccaac atcttcttct ggaggccgtg gttggcttgt atggagcagc agacgcgcta
     1801 cttcgagcgg aggcatccgg agcttgcagg atcgccacga ctccgggcgt atatgctccg
     1861 cattggtctt gaccaactct atcagagctt ggttgacggc aatttcgatg atgcagcttg
     1921 ggcgcagggt cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca
     1981 aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag
     2041 tggaaaccga cgccccagca ctcgtccgag ggcaaagaaa tagagtagat gccgaccggg
     2101 atctgtcgat cgacaagctc gagtttctcc ataataatgt gtgagtagtt cccagataag
     2161 ggaattaggg ttcctatagg gtttcgctca tgtgttgagc atataagaaa cccttagtat
     2221 gtatttgtat ttgtaaaata cttctatcaa taaaatttct aattcctaaa accaaaatcc
     2281 agtactaaaa tccagatccc ccgaattaat tcggcgttaa ttcagtacat taaaaacgtc
     2341 cgcaatgtgt tattaagttg tctaagcgtc aatttgttta caccacaata tatcctgcca
     2401 ccagccagcc aacagctccc cgaccggcag ctcggcacaa aatcaccact cgatacaggc
     2461 agcccatcag tccgggacgg cgtcagcggg agagccgttg taaggcggca gactttgctc
     2521 atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa acacggatga
     2581 tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc tgtgatcacc
     2641 gcggtttcaa aatcggctcc gtcgatacta tgttatacgc caactttgaa aacaactttg
     2701 aaaaagctgt tttctggtat ttaaggtttt agaatgcaag gaacagtgaa ttggagttcg
     2761 tcttgttata attagggaag gtgcgaacaa gtccctgata tgagatcatg tttgtcatct
     2821 ggagccatag aacagggttc atcatgagtc atcaacttac cttcgccgac agtgaattca
     2881 gcagtaagcg ccgtcagacc agaaaagaga ttttcttgtc ccgcatggag cagattctgc
     2941 catggcaaaa catggtggaa gtcatcgagc cgttttaccc caaggctggt aatggccggc
     3001 gaccttatcc gctggaaacc atgctacgca ttcactgcat gcagcattgg tacaacctga
     3061 gcgatggcgc gatggaagat gctctgtacg aaatcgcctc catgcgtctg tttgcccggt
     3121 tatccctgga tagcgccttg ccggaccgca ccaccatcat gaatttccgc cacctgctgg
     3181 agcagcatca actggcccgc caattgttca agaccatcaa tcgctggctg gccgaagcag
     3241 gcgtcatgat gactcaaggc accttggtcg atgccaccat cattgaggca cccagctcga
     3301 ccaagaacaa agagcagcaa cgcgatccgg agatgcatca gaccaagaaa ggcaatcagt
     3361 ggcactttgg catgaaggcc cacattggtg tcgatgccaa gagtggcctg acccacagcc
     3421 tggtcaccac cgcggccaac gagcatgacc tcaatcagct gggtaatctg ctgcatggag
     3481 aggagcaatt tgtctcagcc gatgccggct accaaggggc gccacagcgc gaggagctgg
     3541 ccgaggtgga tgtggactgg ctgatcgccg agcgccccgg caaggtaaga accttgaaac
     3601 agcatccacg caagaacaaa acggccatca acatcgaata catgaaagcc agcatccggg
     3661 ccagggtgga gcacccattt cgcatcatca agcgacagtt cggcttcgtg aaagccagat
     3721 acaaggggtt gctgaaaaac gataaccaac tggcgatgtt attcacgctg gccaacctgt
     3781 ttcgggcgga ccaaatgata cgtcagtggg agagatctca ctaaaaactg gggataacgc
     3841 cttaaatggc gaagaaacgg tctaaatagg ctgattcaag gcatttacgg gagaaaaaat
     3901 cggctcaaac atgaagaaat gaaatgactg agtcagccga gaagaatttc cccgcttatt
     3961 cgcaccttcc ttagcttctt ggggtatctt taaatactgt agaaaagagg aaggaaataa
     4021 taaatggcta aaatgagaat atcaccggaa ttgaaaaaac tgatcgaaaa ataccgctgc
     4081 gtaaaagata cggaaggaat gtctcctgct aaggtatata agctggtggg agaaaatgaa
     4141 aacctatatt taaaaatgac ggacagccgg tataaaggga ccacctatga tgtggaacgg
     4201 gaaaaggaca tgatgctatg gctggaagga aagctgcctg ttccaaaggt cctgcacttt
     4261 gaacggcatg atggctggag caatctgctc atgagtgagg ccgatggcgt cctttgctcg
     4321 gaagagtatg aagatgaaca aagccctgaa aagattatcg agctgtatgc ggagtgcatc
     4381 aggctctttc actccatcga catatcggat tgtccctata cgaatagctt agacagccgc
     4441 ttagccgaat tggattactt actgaataac gatctggccg atgtggattg cgaaaactgg
     4501 gaagaagaca ctccatttaa agatccgcgc gagctgtatg attttttaaa gacggaaaag
     4561 cccgaagagg aacttgtctt ttcccacggc gacctgggag acagcaacat ctttgtgaaa
     4621 gatggcaaag taagtggctt tattgatctt gggagaagcg gcagggcgga caagtggtat
     4681 gacattgcct tctgcgtccg gtcgatcagg gaggatatcg gggaagaaca gtatgtcgag
     4741 ctattttttg acttactggg gatcaagcct gattgggaga aaataaaata ttatatttta
     4801 ctggatgaat tgttttagta cctagaatgc atgaccaaaa tcccttaacg tgagttttcg
     4861 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     4921 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     4981 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     5041 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     5101 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     5161 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     5221 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     5281 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     5341 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     5401 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     5461 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     5521 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     5581 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     5641 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt
     5701 acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat
     5761 gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc
     5821 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     5881 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     5941 caccgaaacg cgcgaggcag ggtgccttga tgtgggcgcc ggcggtcgag tggcgacggc
     6001 gcggcttgtc cgcgccctgg tagattgcct ggccgtaggc cagccatttt tgagcggcca
     6061 gcggccgcga taggccgacg cgaagcggcg gggcgtaggg agcgcagcga ccgaagggta
     6121 ggcgcttttt gcagctcttc ggctgtgcgc tggccagaca gttatgcaca ggccaggcgg
     6181 gttttaagag ttttaataag ttttaaagag ttttaggcgg aaaaatcgcc ttttttctct
     6241 tttatatcag tcacttacat gtgtgaccgg ttcccaatgt acggctttgg gttcccaatg
     6301 tacgggttcc ggttcccaat gtacggcttt gggttcccaa tgtacgtgct atccacagga
     6361 aacagacctt ttcgaccttt ttcccctgct agggcaattt gccctagcat ctgctccgta
     6421 cattaggaac cggcggatgc ttcgccctcg atcaggttgc ggtagcgcat gactaggatc
     6481 gggccagcct gccccgcctc ctccttcaaa tcgtactccg gcaggtcatt tgacccgatc
     6541 agcttgcgca cggtgaaaca gaacttcttg aactctccgg cgctgccact gcgttcgtag
     6601 atcgtcttga acaaccatct ggcttctgcc ttgcctgcgg cgcggcgtgc caggcggtag
     6661 agaaaacggc cgatgccggg atcgatcaaa aagtaatcgg ggtgaaccgt cagcacgtcc
     6721 gggttcttgc cttctgtgat ctcgcggtac atccaatcag ctagctcgat ctcgatgtac
     6781 tccggccgcc cggtttcgct ctttacgatc ttgtagcggc taatcaaggc ttcaccctcg
     6841 gataccgtca ccaggcggcc gttcttggcc ttcttcgtac gctgcatggc aacgtgcgtg
     6901 gtgtttaacc gaatgcaggt ttctaccagg tcgtctttct gctttccgcc atcggctcgc
     6961 cggcagaact tgagtacgtc cgcaacgtgt ggacggaaca cgcggccggg cttgtctccc
     7021 ttcccttccc ggtatcggtt catggattcg gttagatggg aaaccgccat cagtaccagg
     7081 tcgtaatccc acacactggc catgccggcc ggccctgcgg aaacctctac gtgcccgtct
     7141 ggaagctcgt agcggatcac ctcgccagct cgtcggtcac gcttcgacag acggaaaacg
     7201 gccacgtcca tgatgctgcg actatcgcgg gtgcccacgt catagagcat cggaacgaaa
     7261 aaatctggtt gctcgtcgcc cttgggcggc ttcctaatcg acggcgcacc ggctgccggc
     7321 ggttgccggg attctttgcg gattcgatca gcggccgctt gccacgattc accggggcgt
     7381 gcttctgcct cgatgcgttg ccgctgggcg gcctgcgcgg ccttcaactt ctccaccagg
     7441 tcatcaccca gcgccgcgcc gatttgtacc gggccggatg gtttgcgacc gctcacgccg
     7501 attcctcggg cttgggggtt ccagtgccat tgcagggccg gcaggcaacc cagccgctta
     7561 cgcctggcca accgcccgtt cctccacaca tggggcattc cacggcgtcg gtgcctggtt
     7621 gttcttgatt ttccatgccg cctcctttag ccgctaaaat tcatctactc atttattcat
     7681 ttgctcattt actctggtag ctgcgcgatg tattcagata gcagctcggt aatggtcttg
     7741 ccttggcgta ccgcgtacat cttcagcttg gtgtgatcct ccgccggcaa ctgaaagttg
     7801 acccgcttca tggctggcgt gtctgccagg ctggccaacg ttgcagcctt gctgctgcgt
     7861 gcgctcggac ggccggcact tagcgtgttt gtgcttttgc tcattttctc tttacctcat
     7921 taactcaaat gagttttgat ttaatttcag cggccagcgc ctggacctcg cgggcagcgt
     7981 cgccctcggg ttctgattca agaacggttg tgccggcggc ggcagtgcct gggtagctca
     8041 cgcgctgcgt gatacgggac tcaagaatgg gcagctcgta cccggccagc gcctcggcaa
     8101 cctcaccgcc gatgcgcgtg cctttgatcg cccgcgacac gacaaaggcc gcttgtagcc
     8161 ttccatccgt gacctcaatg cgctgcttaa ccagctccac caggtcggcg gtggcccata
     8221 tgtcgtaagg gcttggctgc accggaatca gcacgaagtc ggctgccttg atcgcggaca
     8281 cagccaagtc cgccgcctgg ggcgctccgt cgatcactac gaagtcgcgc cggccgatgg
     8341 ccttcacgtc gcggtcaatc gtcgggcggt cgatgccgac aacggttagc ggttgatctt
     8401 cccgcacggc cgcccaatcg cgggcactgc cctggggatc ggaatcgact aacagaacat
     8461 cggccccggc gagttgcagg gcgcgggcta gatgggttgc gatggtcgtc ttgcctgacc
     8521 cgcctttctg gttaagtaca gcgataacct tcatgcgttc cccttgcgta tttgtttatt
     8581 tactcatcgc atcatatacg cagcgaccgc atgacgcaag ctgttttact caaatacaca
     8641 tcaccttttt agacggcggc gctcggtttc ttcagcggcc aagctggccg gccaggccgc
     8701 cagcttggca tcagacaaac cggccaggat ttcatgcagc cgcacggttg agacgtgcgc
     8761 gggcggctcg aacacgtacc cggccgcgat catctccgcc tcgatctctt cggtaatgaa
     8821 aaacggttcg tcctggccgt cctggtgcgg tttcatgctt gttcctcttg gcgttcattc
     8881 tcggcggccg ccagggcgtc ggcctcggtc aatgcgtcct aggcaccgcg ccgcctggcc
     8941 tcggtgggcg tcacttcctc gctgcgctca agtgcgcggt acagggtcga gcgatgcacg
     9001 ccaagcagtg cagccgcctc tttcacggtg cggccttcct ggtcgatcag ctcgcgggcg
     9061 tgcgcgatct gtgccggggt gagggtaggg cgggggccaa acttcacgcc tcgggccttg
     9121 gcggcctcgc gcccgctccg ggtgcggtcg atgattaggg aacgctcgaa ctcggcaatg
     9181 ccggcgaaca cggtcaacac catgcggccg gccggcgtgg tggtgtcggc ccacggctct
     9241 gccaggctac gcaggcccgc gccggcctcc tggatgcgct cggcaatgtc cagtaggtcg
     9301 cgggtgctgc gggccaggcg gtctagcctg gtcactgtca caacgtcgcc agggcgtagg
     9361 tggtcaagca tcctggccag ctccgggcgg tcgcgcctgg tgccggtgat cttctcggaa
     9421 aacagcttgg tgcagccggc cgcgtgcagt tcggcccgtt ggttggtcaa gtcctggtcg
     9481 tcggtgctga cgcgggcata gcccagcagg ccagcggcgg cgctcttgtt catggcgtaa
     9541 tgtctccggt tctagtcgca agtattctac tttatgcgac taaaacacgc gacaagaaaa
     9601 cgccaggaaa agggcagggc ggcagcctgt cgcgtaactt aggacttgtg cgacatgtcg
     9661 ttttcagaag acggctgcac tgaacgtcag aagccgactg cactatagca gcggaggggt
     9721 tggatcaaag tactttgatc ccgaggggaa ccctgtggtt ggcatgcaca tacaaatgga
     9781 cgaacggata aaccttttca cgccctttta aatatccgat tattctaata aacgctcttt
     9841 tctcttaggt ttacccgcca atatatcctg tcaaacactg atagtttaaa ctgaaggcgg
     9901 gaaacgacaa tctgatccaa gctcaagctg ctctagcatt cgccattcag gctgcgcaac
     9961 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
    10021 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
    10081 acgacggcca gtgccaagct tgcatgcggt caacatggtg gagcacgaca cacttgtcta
    10141 ctccaaaaat atcaaagata cagtctcaga agaccaaagg gcaattgaga cttttcaaca
    10201 aagggtaata tccggaaacc tcctcggatt ccattgccca gctatctgtc actttattgt
    10261 gaagatagtg gaaaaggaag gtggctccta caaatgccat cattgcgata aaggaaaggc
    10321 catcgttgaa gatgcctctg ccgacagtgg tcccaaagat ggacccccac ccacgaggag
    10381 catcgtggaa aaagaagacg ttccaaccac gtcttcaaag caagtggatt gatgtgataa
    10441 catggtggag cacgacacac ttgtctactc caaaaatatc aaagatacag tctcagaaga
    10501 ccaaagggca attgagactt ttcaacaaag ggtaatatcc ggaaacctcc tcggattcca
    10561 ttgcccagct atctgtcact ttattgtgaa gatagtggaa aaggaaggtg gctcctacaa
    10621 atgccatcat tgcgataaag gaaaggccat cgttgaagat gcctctgccg acagtggtcc
    10681 caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc
    10741 ttcaaagcaa gtggattgat gtgatatctc cactgacgta agggatgacg cacaatccca
    10801 ctatccttcg caagaccctt cctctatata aggaagttca tttcatttgg agaggacctc
    10861 gactctagag gatccccggg taccgggccc cccctcgagg cgcgccaagc tatcaaacaa
    10921 gtttgtacaa aaaagcaggc tccgaattcg cccttcacca tggattacaa ggaccacgac
    10981 ggggattaca aggaccacga cattgattac aaggatgatg atgacaagat ggctccgaag
    11041 aagaagagga aggttggcat ccacggggtg ccagctgctg acaagaagta ctcgatcggc
    11101 ctcgctattg ggactaactc tgttggctgg gccgtgatca ccgacgagta caaggtgccc
    11161 tcaaagaagt tcaaggtcct gggcaacacc gatcggcatt ccatcaagaa gaatctcatt
    11221 ggcgctctcc tgttcgacag cggcgagacg gctgaggcta cgcggctcaa gcgcaccgcc
    11281 cgcaggcggt acacgcgcag gaagaatcgc atctgctacc tgcaggagat tttctccaac
    11341 gagatggcga aggttgacga ttctttcttc cacaggctgg aggagtcatt cctcgtggag
    11401 gaggataaga agcacgagcg gcatccaatc ttcggcaaca ttgtcgacga ggttgcctac
    11461 cacgagaagt accctacgat ctaccatctg cggaagaagc tcgtggactc cacagataag
    11521 gcggacctcc gcctgatcta cctcgctctg gcccacatga ttaagttcag gggccatttc
    11581 ctgatcgagg gggatctcaa cccggacaat agcgatgttg acaagctgtt catccagctc
    11641 gtgcagacgt acaaccagct cttcgaggag aaccccatta atgcgtcagg cgtcgacgcg
    11701 aaggctatcc tgtccgctag gctctcgaag tctcggcgcc tcgagaacct gatcgcccag
    11761 ctgccgggcg agaagaagaa cggcctgttc gggaatctca ttgcgctcag cctggggctc
    11821 acgcccaact tcaagtcgaa tttcgatctc gctgaggacg ccaagctgca gctctccaag
    11881 gacacatacg acgatgacct ggataacctc ctggcccaga tcggcgatca gtacgcggac
    11941 ctgttcctcg ctgccaagaa tctgtcggac gccatcctcc tgtctgatat tctcagggtg
    12001 aacaccgaga ttacgaaggc tccgctctca gcctccatga tcaagcgcta cgacgagcac
    12061 catcaggatc tgaccctcct gaaggcgctg gtcaggcagc agctccccga gaagtacaag
    12121 gagatcttct tcgatcagtc gaagaacggc tacgctgggt acattgacgg cggggcctct
    12181 caggaggagt tctacaagtt catcaagccg attctggaga agatggacgg cacggaggag
    12241 ctgctggtga agctcaatcg cgaggacctc ctgaggaagc agcggacatt cgataacggc
    12301 agcatcccac accagattca tctcggggag ctgcacgcta tcctgaggag gcaggaggac
    12361 ttctaccctt tcctcaagga taaccgcgag aagatcgaga agattctgac tttcaggatc
    12421 ccgtactacg tcggcccact cgctaggggc aactcccgct tcgcttggat gacccgcaag
    12481 tcagaggaga cgatcacgcc gtggaacttc gaggaggtgg tcgacaaggg cgctagcgct
    12541 cagtcgttca tcgagaggat gacgaatttc gacaagaacc tgccaaatga gaaggtgctc
    12601 cctaagcact cgctcctgta cgagtacttc acagtctaca acgagctgac taaggtgaag
    12661 tatgtgaccg agggcatgag gaagccggct ttcctgtctg gggagcagaa gaaggccatc
    12721 gtggacctcc tgttcaagac caaccggaag gtcacggtta agcagctcaa ggaggactac
    12781 ttcaagaaga ttgagtgctt cgattcggtc gagatctctg gcgttgagga ccgcttcaac
    12841 gcctccctgg ggacctacca cgatctcctg aagatcatta aggataagga cttcctggac
    12901 aacgaggaga atgaggatat cctcgaggac attgtgctga cactcactct gttcgaggac
    12961 cgggagatga tcgaggagcg cctgaagact tacgcccatc tcttcgatga caaggtcatg
    13021 aagcagctca agaggaggag gtacaccggc tgggggaggc tgagcaggaa gctcatcaac
    13081 ggcattcggg acaagcagtc cgggaagacg atcctcgact tcctgaagag cgatggcttc
    13141 gcgaaccgca atttcatgca gctgattcac gatgacagcc tcacattcaa ggaggatatc
    13201 cagaaggctc aggtgagcgg ccagggggac tcgctgcacg agcatatcgc gaacctcgct
    13261 ggctcgccag ctatcaagaa ggggattctg cagaccgtga aggttgtgga cgagctggtg
    13321 aaggtcatgg gcaggcacaa gcctgagaac atcgtcattg agatggcccg ggagaatcag
    13381 accacgcaga agggccagaa gaactcacgc gagaggatga agaggatcga ggagggcatt
    13441 aaggagctgg ggtcccagat cctcaaggag cacccggtgg agaacacgca gctgcagaat
    13501 gagaagctct acctgtacta cctccagaat ggccgcgata tgtatgtgga ccaggagctg
    13561 gatattaaca ggctcagcga ttacgacgtc gatgctatcg ttccacagtc attcctgaag
    13621 gatgactcca ttgacaacaa ggtcctcacc aggtcggaca agaaccgggg caagtctgat
    13681 aatgttcctt cagaggaggt cgttaagaag atgaagaact actggcgcca gctcctgaat
    13741 gccaagctga tcacgcagcg gaagttcgat aacctcacaa aggctgagag gggcgggctc
    13801 tctgagctgg acaaggcggg cttcatcaag aggcagctgg tcgagacacg gcagatcact
    13861 aagcacgttg cgcagattct cgactcacgg atgaacacta agtacgatga gaatgacaag
    13921 ctgatccgcg aggtgaaggt catcaccctg aagtcaaagc tcgtctccga cttcaggaag
    13981 gatttccagt tctacaaggt tcgggagatc aacaattacc accatgccca tgacgcgtac
    14041 ctgaacgcgg tggtcggcac agctctgatc aagaagtacc caaagctcga gagcgagttc
    14101 gtgtacgggg actacaaggt ttacgatgtg aggaagatga tcgccaagtc ggagcaggag
    14161 attggcaagg ctaccgccaa gtacttcttc tactctaaca ttatgaattt cttcaagaca
    14221 gagatcactc tggccaatgg cgagatccgg aagcgccccc tcatcgagac gaacggcgag
    14281 acgggggaga tcgtgtggga caagggcagg gatttcgcga ccgtcaggaa ggttctctcc
    14341 atgccacaag tgaatatcgt caagaagaca gaggtccaga ctggcgggtt ctctaaggag
    14401 tcaattctgc ctaagcggaa cagcgacaag ctcatcgccc gcaagaagga ctgggatccg
    14461 aagaagtacg gcgggttcga cagccccact gtggcctact cggtcctggt tgtggcgaag
    14521 gttgagaagg gcaagtccaa gaagctcaag agcgtgaagg agctgctggg gatcacgatt
    14581 atggagcgct ccagcttcga gaagaacccg atcgatttcc tggaggcgaa gggctacaag
    14641 gaggtgaaga aggacctgat cattaagctc cccaagtact cactcttcga gctggagaac
    14701 ggcaggaagc ggatgctggc ttccgctggc gagctgcaga aggggaacga gctggctctg
    14761 ccgtccaagt atgtgaactt cctctacctg gcctcccact acgagaagct caagggcagc
    14821 cccgaggaca acgagcagaa gcagctgttc gtcgagcagc acaagcatta cctcgacgag
    14881 atcattgagc agatttccga gttctccaag cgcgtgatcc tggccgacgc gaatctggat
    14941 aaggtcctct ccgcgtacaa caagcaccgc gacaagccaa tcagggagca ggctgagaat
    15001 atcattcatc tcttcaccct gacgaacctc ggcgcccctg ctgctttcaa gtacttcgac
    15061 acaactatcg atcgcaagag gtacacaagc actaaggagg tcctggacgc gaccctcatc
    15121 caccagtcga ttaccggcct ctacgagacg cgcatcgacc tgtctcagct cgggggcgac
    15181 aagcggccag cggcgacgaa gaaggcgggg caggcgaaga agaagaagaa gggagacggc
    15241 tctggatcgg ggtcgggttc tggctcagtc gacgatgctc ttgacgattt tgacctcgat
    15301 atgctcgacg ctcttgatga ttttgatctc gacatgctcg atgcacttga tgactttgac
    15361 cttgacatgc tcgacgcact cgatgacttc gacctcgaca tgcttgaggg cagaggaagt
    15421 cttctaacat gcggtgacgt ggaggagaat cccggcccta tggcgtcaaa tttcacgcag
    15481 tttgttttgg ttgataacgg cgggactggc gacgttacag tagctccatc aaattttgcg
    15541 aacggagtcg ctgagtggat tagctcaaat tcaaggtccc aggcctacaa ggttacctgt
    15601 tctgttaggc agagttctgc gcaaaaaaga aaatatacca tcaaggttga agtccctaaa
    15661 gttgcaacac aaacagtcgg tggtgttgag ctccctgtgg cagcctggag atcttactta
    15721 aacatggagc taacaattcc aatattcgct acaaactctg attgtgaact gattgttaag
    15781 gcgatgcaag gcctcttgaa agatggaaac cctataccgt ccgctatcgc agctaacagc
    15841 ggtatctatc ctaagaagaa gaggaaggtt ggctctggat cggggtcggg ttctggctca
    15901 ggatccggta cctatcccta tgacgtgccc gattatgcca gcctgggcag cggctccccc
    15961 aagaaaaaac gcaaggtgga agatcctaag aaaaagcgga aagtggacgg cattggtagt
    16021 gggagcaacg gcagcagcgg atccaacggt ccgactgacg ccgcggaaga agaacttttg
    16081 agcaagaatt atcatcttga gaacgaagtg gctcgtctta agaaaggttc tggcagtgga
    16141 gaagaactgc tttcaaagaa ttaccacctg gaaaatgagg tagctagact gaaaaagggg
    16201 agcggaagtg gggaggagtt gctgagcaaa aattatcatt tggagaacga agtagcacga
    16261 ctaaagaaag ggtccggatc gggtgaggag ttactctcga aaaattatca tctcgaaaac
    16321 gaagtggctc ggctaaaaaa gggcagtggt tctggagaag agctattatc taaaaactac
    16381 cacctcgaaa atgaggtggc acgcttaaaa aagggaagtg gcagtggtga agagctacta
    16441 tccaagaatt atcatcttga gaacgaggta gcgcgtttga agaagggttc cggctcagga
    16501 gaggaactgc tctcgaagaa ctatcatctt gaaaatgagg tcgctcgatt aaaaaaggga
    16561 tcgggcagtg gtgaggaact actttcaaag aattaccacc tcgaaaacga agtagctcga
    16621 ttaaagaaag gttcagggtc gggtgaagaa ttactgagta aaaattatca tctggaaaat
    16681 gaggtagcga gactaaaaaa ggggagtggt tctggcgagg aattgctatc gaaaaattat
    16741 catcttgaga acgaagttgc taggctcaaa aagggctcag gctcaggcac cgcggtaaac
    16801 ataggtggtg gaaccggtcc gatggatcta cagcggccgc aaggtggagg tggacccaag
    16861 aagaagcgca aggtgtgaac tagttaaaga gctttcgttc gtatcatcgg tttcgacaac
    16921 gttcgtcaag ttcaatgcat cagtttcatt gcgcacacac cagaatccta ctgagtttga
    16981 gtattatggc attgggaaaa ctgtttttct tgtaccattt gttgtgcttg taatttactg
    17041 tgttttttat tcggttttcg ctatcgaact gtgaaatgga aatggatgga gaagagttaa
    17101 tgaatgatat ggtccttttg ttcattctca aattaatatt atttgttttt tctcttattt
    17161 gttgtgtgtt gaatttgaaa ttataagaga tatgcaaaca ttttgttttg agtaaaaatg
    17221 tgtcaaatcg tggcctctaa tgaccgaagt taatatgagg agtaaaacac ttgtagttgt
    17281 accattatgc ttattcacta ggcaacaaat atattttcag acctagaaaa gctgcaaatg
    17341 ttactgaata caagtatgtc ctcttgtgtt ttagacattt atgaactttc ctttatgtaa
    17401 ttttccagaa tccttgtcag attctaatca ttgctttata attatagtta tactcatgga
    17461 tttgtagttg agtatgaaaa tattttttaa tgcattttat gacttgcccg tacgctgcag
    17521 tgcagcgtga cccggtcgtg cccctctcta gagataatga gcattgcatg tctaagttat
    17581 aaaaaattac cacatatttt ttttgtcaca cttgtttgaa gtgcagttta tctatcttta
    17641 tacatatatt taaactttac tctacgaata atataatcta tagtactaca ataatatcag
    17701 tgttttagag aatcatataa atgaacagtt agacatggtc taaaggacaa ttgagtattt
    17761 tgacaacagg actctacagt tttatctttt tagtgtgcat gtgttctcct ttttttttgc
    17821 aaatagcttc acctatataa tacttcatcc attttattag tacatccatt tagggtttag
    17881 ggttaatggt ttttatagac taattttttt agtacatcta ttttattcta ttttagcctc
    17941 taaattaaga aaactaaaac tctattttag tttttttatt taataattta gatataaaat
    18001 agaataaaat aaagtgacta aaaattaaac aaataccctt taagaaatta aaaaaactaa
    18061 ggaaacattt ttcttgtttc gagtagataa tgccagcctg ttaaacgccg tcgacgagtc
    18121 taacggacac caaccagcga accagcagcg tcgcgtcggg ccaagcgaag cagacggcac
    18181 ggcatctctg tcgctgcctc tggacccctc tcgagagttc cgctccaccg ttggacttgc
    18241 tccgctgtcg gcatccagaa attgcgtggc ggagcggcag acgtgagccg gcacggcagg
    18301 cggcctcctc ctcctctcac ggcaccggca gctacggggg attcctttcc caccgctcct
    18361 tcgctttccc ttcctcgccc gccgtaataa atagacaccc cctccacacc ctctttcccc
    18421 aacctcgtgt tgttcggagc gcacacacac acaaccagat ctcccccaaa tccacccgtc
    18481 ggcacctccg cttcaaggta cgccgctcgt cctccccccc ccccccctct ctaccttctc
    18541 tagatcggcg ttccggtcca tggttagggc ccggtagttc tacttctgtt catgtttgtg
    18601 ttagatccgt gtttgtgtta gatccgtgct gctagcgttc gtacacggat gcgacctgta
    18661 cgtcagacac gttctgattg ctaacttgcc agtgtttctc tttggggaat cctgggatgg
    18721 ctctagccgt tccgcagacg ggatcgattt catgattttt tttgtttcgt tgcatagggt
    18781 ttggtttgcc cttttccttt atttcaatat atgccgtgca cttgtttgtc gggtcatctt
    18841 ttcatgcttt tttttgtctt ggttgtgatg atgtggtctg gttgggcggt cgttctagat
    18901 cggagtagaa ttaattctgt ttcaaactac ctggtggatt tattaatttt ggatctgtat
    18961 gtgtgtgcca tacatattca tagttacgaa ttgaagatga tggatggaaa tatcgatcta
    19021 ggataggtat acatgttgat gcgggtttta ctgatgcata tacagagatg ctttttgttc
    19081 gcttggttgt gatgatgtgg tgtggttggg cggtcgttca ttcgttctag atcggagtag
    19141 aatactgttt caaactacct ggtgtattta ttaattttgg aactgtatgt gtgtgtcata
    19201 catcttcata gttacgagtt taagatggat ggaaatatcg atctaggata ggtatacatg
    19261 ttgatgtggg ttttactgat gcatatacat gatggcatat gcagcatcta ttcatatgct
    19321 ctaaccttga gtacctatct attataataa acaagtatgt tttataatta ttttgatctt
    19381 gatatacttg gatgatggca tatgcagcag ctatatgtgg atttttttag ccctgccttc
    19441 atacgctatt tatttgcttg gtactgtttc ttttgtcgat gctcaccctg ttgtttggtg
    19501 ttacttctgc agggcgccat ggggccggac atagtcatga cacaaagtcc gagttcgctt
    19561 tctgcgagtg ttggggatag agttacgata acctgcagat ctagtacggg ggccgtcacc
    19621 acgtcgaatt acgcgtcatg ggtccaagaa aagccaggca aactgtttaa gggtcttata
    19681 ggtggcacga acaaccgggc tcccggagtc ccttccaggt ttagtggatc acttattggt
    19741 gacaaggcaa cgctgacgat atcttccctc cagccagaag atttcgcgac ctacttttgt
    19801 gcactttggt attctaacca ctgggtcttt ggccaaggga cgaaggtcga gctcaaacgg
    19861 ggtggcggtg ggagcggcgg gggaggatca gggggcggag gttcgagtgg cggggggagc
    19921 gaagtcaaac tcctcgagtc cggtggagga ttggtgcaac caggtggttc attgaagctc
    19981 tcgtgtgcag tttctggttt ctctttgaca gactacggtg tcaattgggt ccggcaggca
    20041 ccggggcgcg gtcttgagtg gatcggcgtt atctggggcg acggaataac agattataac
    20101 tctgctttga aggacagatt catcattagc aaggacaacg ggaagaacac ggtgtacctc
    20161 cagatgagca aagtgcgcag tgatgatact gccctgtatt actgcgtcac aggtttgttt
    20221 gattactggg gtcaaggcac tcttgtgaca gtctcttcgt atccttatga cgtgcccgac
    20281 tacgctggcg gagggggcgg cagcggaggc ggtggatccg gaggcggtgg ctcagggggc
    20341 ggcgggtcgc ttgatcctgg tggcggaggt tctgggagca aaggagagga gctctttaca
    20401 ggtgtcgtcc ctattttggt ggagcttgat ggagatgtca atggccataa attttccgtc
    20461 aggggcgaag gggaggggga cgcgactaat ggaaaattga ccctcaagtt tatctgcacg
    20521 acaggaaagt tgccggtccc ctggcccact ctggttacca ccctcactta tggggtccag
    20581 tgctttagca gataccctga tcatatgaaa cgccatgact tcttcaaatc agccatgcca
    20641 gagggttatg tgcaggaaag aactataagc tttaaggacg atgggacgta caaaacgcgg
    20701 gcagaggtta agtttgaggg ggatactctt gtgaatagga tcgaacttaa gggcatcgat
    20761 tttaaagaag acggtaatat tcttggccac aagttggagt ataatttcaa ttctcacaac
    20821 gtgtacataa cagcagataa acagaaaaat gggatcaagg ccaattttaa aattaggcat
    20881 aatgttgaag atgggtctgt gcaactggca gatcattatc agcagaatac tccaattggc
    20941 gatggccctg tcttgttgcc tgacaatcat tacctttcca cgcagtcagt cctgtccaaa
    21001 gatccgaatg agaagcggga ccacatggtc ttgctcgaat tcgttactgc cgccggaatc
    21061 actcatggca tggacgagct ttacaaggga ggtggaagga ctggaggtgg cggagggggg
    21121 ctgcttgacc ccggaacccc tatggatgcc gacttggtgg cctcctccac ggttgtttgg
    21181 gaacaggatg ccgacccttt tgcgggcact gctgacgatt ttcccgcttt caatgaggag
    21241 gagttggctt ggctgatgga acttctccca cagggtggta gtggtggcct cctggatccg
    21301 gggacaccga tggacgcaga cctggtggcg tcgtcaacgg ttgtgtggga acaggatgct
    21361 gatccgttcg caggaactgc cgacgatttt cctgcattca acgaagagga actcgcatgg
    21421 ctcatggagc ttctgcctca gggatcgggt ggcgggtcgc gaacggaaga atacaagctt
    21481 atattgaacg gtaagacact gaaaggtgaa actacgacag aagccgttga cgcggcaact
    21541 gcggagaaag tctttaagca gtatgctaac gataatggcg tggacgggga gtggacgtat
    21601 gacgacgcaa ccaaaacatt cacggtcacc gaggggggcg gcagcggagg cggtacctcg
    21661 cccaaaaccc ggagacgccc taggcgctcg cagcgcaaaa gacctcccac gtaaagagct
    21721 ttcgttcgta tcatcggttt cgacaacgtt cgtcaagttc aatgcatcag tttcattgcg
    21781 cacacaccag aatcctactg agtttgagta ttatggcatt gggaaaactg tttttcttgt
    21841 accatttgtt gtgcttgtaa tttactgtgt tttttattcg gttttcgcta tcgaactgtg
    21901 aaatggaaat ggatggagaa gagttaatga atgatatggt ccttttgttc attctcaaat
    21961 taatattatt tgttttttct cttatttgtt gtgtgttgaa tttgaaatta taagagatat
    22021 gcaaacattt tgttttgagt aaaaatgtgt caaatcgtgg cctctaatga ccgaagttaa
    22081 tatgaggagt aaaacacttg tagttgtacc attatgctta ttcactaggc aacaaatata
    22141 ttttcagacc tagaaaagct gcaaatgtta ctgaatacaa gtatgtcctc ttgtgtttta
    22201 gacatttatg aactttcctt tatgtaattt tccagaatcc ttgtcagatt ctaatcattg
    22261 ctttataatt atagttatac tcatggattt gtagttgagt atgaaaatat tttttaatgc
    22321 attttatgac ttgccaattg ttccggaact ctagataagc ttaccggaaa gggcgaattc
    22381 gcaactttgt atacaaaagt tgccccatgg cgttccctct agataacgca ggatcctaaa
    22441 gggatcttta aacatacgaa cagatcactt aaagttcttc tgaagcaact taaagttatc
    22501 aggcatgcat ggatcttgga ggaatcagat gtgcagtcag ggaccatagc acaggacagg
    22561 cgtcttctac tggtgctacc agcaaatgct ggaagccggg aacactgggt acgttggaaa
    22621 ccacgtgatg tggagtaaga taaactgtag gagaaaagca tttcgtagtg ggccatgaag
    22681 cctttcagga catgtattgc agtatgggcc ggcccattac gcaattggac gacaacaaag
    22741 actagtatta gtaccacctc ggctatccac atagatcaaa gctggtttaa aagagttgtg
    22801 cagatgatcc gtggcagaga ccggcgccgc tacagggcgc gtcccattcg ccattcaggc
    22861 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
    22921 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
    22981 gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc gaattgggta
    23041 ccgggccccc cctcgaggtc ctccagcttt tgttcccttt agtgagggtt aattgcgcgc
    23101 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
    23161 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
    23221 ctcacattaa ttgcgttgcg ctcactgccc gctttccacc ggtggtctct gttttagagc
    23281 taggccaaca tgaggatcac ccatgtctgc agggcctagc aagttaaaat aaggctagtc
    23341 cgttatcaac ttggccaaca tgaggatcac ccatgtctgc agggccaagt ggcaccgagt
    23401 cggtgctttt tttgtccctt cgaagggcaa ttctgcagat atccatcaca ctggcggccg
    23461 ctcgaggtcg agggtatcga taagcttgaa ttcgacccag ctttcttgta caaagtggtt
    23521 cgataattct taattaacta gttctagagc ggccgccacc gcggtggagc tcgaatttcc
    23581 ccgatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg
    23641 cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat
    23701 gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat
    23761 acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat
    23821 ctatgttact agatcgggaa tt
//
