LOCUS       sequence-475911-       11777 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express GAL-inducible cytosolic sfCherry3C large fragment and Pex22 
            peroxisomal chauffeur with medium affinity SH3 peptide.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11777)
  TITLE     Dueber Lab plasmids 2025
REFERENCE   2  (bases 1 to 11777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pKS1170
FEATURES             Location/Qualifiers
     source          1..11777
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             732..1172
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             1218..2153
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPGKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             1266..2156
                     /codon_start=1
                     /gene="ESR1"
                     /product="estrogen nuclear receptor alpha ligand-binding 
                     domain"
                     /label=ER-LBD
                     /translation="KRSKKNSLALSLTADQMVSALLDAEPPILYSEYDPTRPFSEASMM
                     GLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMIGLVWRSMEHPGKL
                     LFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCLKSIILLNSGVYTF
                     LSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQLLLILSHIRHMSNK
                     GMEHLYSMKCKNVVPLYDLLLEMLDAHRLHAPTSRGGASVEETDQSHLATAGSTSSHSL
                     QKYYITGEAEGFPATV"
     promoter        3458..3899
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    3458..3575
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     3870..3893
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             5674..6384
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKL
                     ICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEGGNILGHKLEYNYNSHNVYITADKQKNGIKAN
                     FKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     promoter        6962..7369
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     CDS             7370..8464
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     CDS             complement(9495..10310)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(9495..10310)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     10218..10237
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     10302..10321
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      10535..11123
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11024..11043
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 actggccgat aattgcagac gaacgagtcg aacaagaagc aggcaaagtt tagagcactg
       61 cccctccgca ctcaaaaaag aaaaaactag gaggaaaata aaattctcaa ccacacaaac
      121 acataaacac atacaaatac aaatacaagc ttatttactt gacatcgcgc gatcttccac
      181 tattcagcgc cgtccgccct ctctcgtgtt ttttgtttac gcgacaacta tgcgaaatcc
      241 ggagcaacgg gcaaccgttt ggggaaagac cacacccacg cgcgatcgcc atggcaacga
      301 ggtcgcacac gccccacacc cagacctccc tgcgagcggg catgggtaca atgtccccgt
      361 tgccacagac accacttcgt agcacagcgc agagcgtagc gtgttgttgc tgctgacaaa
      421 agaaaatttt tcttagcaaa gcaaaggagg ggaagcacgg gcagatagca ccgtaccata
      481 cccttggaaa ctcgaaatga acgaagcagg aaatgagaga atgagagttt tgtaggtata
      541 tatagcggta gtgtttgcgc gttaccatca tcttctggat ctatctattg ttcttttcct
      601 catcactttc ccctttttcg ctcttcttct tgtcttttat ttctttcttt tttttaattg
      661 ttccctcgat tggctatcta ccaaagaatc caaacttaat acacgtattt atttgtccaa
      721 ttaccagatc tatgaagcta ctgtcttcta tcgaacaagc atgcgatatt tgccgactta
      781 aaaagctcaa gtgctccaaa gaaaaaccga agtgcgccaa gtgtctgaag aacaactggg
      841 agtgtcgcta ctctcccaaa accaaaagat ctccgctgac tagggcacat ctgacagaag
      901 tggaatcaag gctagaaaga ctggaacagc tatttctact gatttttcct cgagaagacc
      961 ttgacatgat tttgaaaatg gattctttac aggatataaa agcattgtta acaggattat
     1021 ttgtacaaga taatgtgaat aaagatgccg tcacagatag attggcttca gtggagactg
     1081 atatgcctct aacattgaga cagcatagaa taagtgcgac atcatcatcg gaagagagta
     1141 gtaacaaagg tcaaagacag ttgactgtat cgattgactc ggcaggatcc ggtgacggtg
     1201 ctggtttaat taactctgct ggagacatga gagctgccaa cctttggcca agcccgctca
     1261 tgatcaaacg ctctaagaag aacagcctgg ccttgtccct gacggccgac cagatggtca
     1321 gtgccttgtt ggatgctgag ccccccatac tctattccga gtatgatcct accagaccct
     1381 tcagtgaagc ttcgatgatg ggcttactga ccaacctggc agacagggag ctggttcaca
     1441 tgatcaactg ggcgaagagg gtgccaggct ttgtggattt gaccctccat gatcaggtcc
     1501 accttctaga atgtgcctgg ctagagatcc tgatgattgg actcgtctgg cgctccatgg
     1561 agcacccagg gaagctactg tttgctccta acttgctctt ggacaggaac cagggaaaat
     1621 gtgtagaggg catggtggag atcttcgaca tgctgctggc tacatcatct cggttccgca
     1681 tgatgaatct gcagggagag gagtttgtgt gcctcaaatc tattattttg cttaattctg
     1741 gagtgtacac atttctgtcc agcaccctga agtctctgga agagaaggac catatccacc
     1801 gagtcctgga caagatcaca gacactttga tccacctgat ggccaaggca ggcctgaccc
     1861 tgcagcagca gcaccagcgg ctggcccagc tcctcctcat cctctcccac atcaggcaca
     1921 tgagtaacaa aggcatggag catctgtaca gcatgaagtg caagaacgtg gtgcccctct
     1981 atgacctgct gctggagatg ctggacgccc accgcctaca tgcgcccact agccgtggag
     2041 gggcatccgt ggaggaaacg gaccaaagcc acttggccac tgcgggctct acttcatcgc
     2101 attccttgca aaagtattac atcacggggg aggcagaggg tttccctgcc acagtcgcgg
     2161 ctgcaggtga cggtgctggt ttaattaaca tgacggtcga ccatgatttc aatagcgaag
     2221 atattttatt ccccatagaa agcatgagta gtatacaata cgtggagaat aataacccaa
     2281 ataatattaa caacgatgtt atcccgtatt ctctagatat caaaaacact gtcttagata
     2341 gtgcggatct caatgacatt caaaatcaag aaacttcact gaatttgggg cttcctccac
     2401 tatctttcga ctctccactg cccgtaacgg aaacgatacc atccactacc gataacagct
     2461 tgcatttgaa agctgatagc aacaaaaatc gcgatgcaag aactattgaa aatgatagtg
     2521 aaattaagag tactaataat gctagtggct ctggggcaaa tcaatacaca actcttactt
     2581 caccttatcc tatgaacgac attttgtaca acatgaacaa tccgttacaa tcaccgtcac
     2641 cttcatcggt acctcaaaat ccgactataa atcctcccat aaatacagca agtaacgaaa
     2701 ctaatttatc gcctcaaact tcaaatggta atgaaactct tatatctcct cgagcccaac
     2761 aacatacgtc cattaaagat aatcgtctgt ccttacctaa tggtgctaat tcgaatcttt
     2821 tcattgacac taacccaaac aatttgaacg aaaaactaag aaatcaattg aactcagata
     2881 caaattcata ttctaactcc atttctaatt caaactccaa ttctacgggt aatttaaatt
     2941 ccagttattt taattcactg aacatagact ccatgctaga tgattacgtt tctagtgatc
     3001 tcttattgaa tgatgatgat gatgacacta atttatcacg ccgaagattt agcgacgtta
     3061 taacaaacca atttccgtca atgacaaatt cgaggaatga gctcggatcc taactcgaga
     3121 ttgaattgaa ttgaaatcga tagatcaatt tttttctttt ctctttcccc atcctttacg
     3181 ctaaaataat agtttatttt attttttgaa tattttttat ttatatacgt atatatagac
     3241 tattatttat cttttaatga ttattaagat ttttattaaa aaaaaattcg ctcctctttt
     3301 aatgccttta tgcagttttt ttttcccatt cgatatttct atgtgctgcc aaaccagatg
     3361 tcaacacagc tacaacgccc cattatctta gcctaaaaaa accttctctt tggaactttc
     3421 agtaatacgc ttaactgctc attgctatat tgaagtacgg attagaagcc gccgagcggg
     3481 cgacagccct ccgacggatg actctcctcc gtgcgtcctc gtcatcaccg gtcgcgttcc
     3541 tgaaacgcag atgtgcctcg cgccgcactg ctccgaacaa taaagattct acaatactag
     3601 cttttatggt tatgaagagg aaaaattggc agtaacctgg ccccacaaac cttcaaatta
     3661 acgaatcaaa ttaacaacca taggatgata atgcgattag ttttttagcc ttatttctgg
     3721 ggtaattaat cagcgaagcg atgatttttg atctattaac agatatataa atggaaaagc
     3781 tgcataacca ctttaactaa tactttcaac attttcagtt tgtattactt cttattcaaa
     3841 tgtcataaaa gtatcaacaa aaaattgtta atatacctct atactttaac gtcaaggaga
     3901 aaaaactata agatctatgg aggaagacaa catggctatc atcaaggaat tcatgagatt
     3961 caaggtccac atggaaggtt ctgtcaacgg tcacgaattt gaaattgaag gtgaaggtga
     4021 aggtcatcca tacgaaggta ctcaaacagc tagactaaag gttaccaagg gtgacccatt
     4081 gccttttgct tgggatatct tgtctccaca attcatgtac ggttccaagg cttacgttaa
     4141 gcacccagct gacattccag actacttgaa attatctttc ccagaaggtt tcacctggga
     4201 aagagttatg aacttcgaag acggcggagt tgttgctgtc acccaagact cctccttgca
     4261 agatggtcaa ttcatctaca aggtcaagtt gttgggtatt aatttcccat cagatggtcc
     4321 agtcatgcaa aagaagacca tgggttggga agcttccact gaaagaatgt acccagaaga
     4381 tggtgctttg aagggtgaaa tcaaccaaag attgaaatta aaggacggtg gtcactacga
     4441 tgctgaagtc aagaccactt acagagccaa gaagccagtt caattgccag gtgcctacga
     4501 tgttgacatt aaattggaca tcacttctca caacgaagat ggatccggtt cattgggaag
     4561 aggtagaaga tccaaaacct aataactcga gtggcagctt ttgattaagc cttctagtcc
     4621 aaaaaacacg tttttttgtc atttatttca ttttcttaga atagtttagt ttattcattt
     4681 tatagtcacg aatgttttat gattctatat agggttgcaa acaagcattt ttcattttat
     4741 gttaaaacaa tttcaggttt accttttatt ctgcttgtgg tgacgcgtgt atccgcccgc
     4801 tcttttggtc acccatgtat gctggatgca cacactggct taagatgaca acgtcttggg
     4861 gccttaccac cagtggactt tcttgctgtt tgctttgttc tggccattgt ttgcgtttat
     4921 atatttatgt tagatgtttt tcttattaac tagaaagaaa gaatataaaa ggttgaggaa
     4981 agagatgtat cccgaagaat acacagtctt ttatatatgt atttcaacaa ggagccgtgg
     5041 agggtactaa aaagaaaaat cgcccgggca tttcgttatc ttccacgcta aaagtcaagg
     5101 agagatatta cggccaggat cgcaaaggtg cagagcaagg aaatgtgaga aattgtgaga
     5161 acgataatgt atgggacaat gcgaaaatgt gagaacgaga gcaaaaatct tttttgtatc
     5221 tccccgccga atttggaaac cgcgttctga aaacttcgca tcttcacata gtaaaactgt
     5281 tccgagcgct tctccccata atggttagtg gtaaaaaccg aagttgttta ctttagcaaa
     5341 tgcccgcgaa tacggtggta aattgccacc cccccttccc cattcattgg gtaaagacca
     5401 atttgatgga taaattggtt gtggaaaagg tctaattctt tttcctataa ataccgagat
     5461 attttttcta tatgatggtt tccgtcgcat tattgtactc tatagtacta aagcaacaaa
     5521 caaaaacaag caacaaatat aatatagtaa aatagatcta tgccaccacc atcaagaagt
     5581 agaataaaca aaacaagaac attaggaata gtgggtacag ctatagcagt gttggtcacg
     5641 tcctactata tatatcaaaa ggtgacaggt tcttctaaag gtgaagaatt attcactggt
     5701 gttgtcccaa ttttggttga attagatggt gatgttaatg gtcacaaatt ttctgtctcc
     5761 ggtgaaggtg aaggtgatgc tacttacggt aaattgacct taaaattgat ttgtactact
     5821 ggtaaattgc cagttccatg gccaacctta gtcactactt taggttatgg tttgcaatgt
     5881 tttgctagat acccagatca tatgaaacaa catgactttt tcaagtctgc catgccagaa
     5941 ggttatgttc aagaaagaac tatttttttc aaagatgacg gtaactacaa gaccagagct
     6001 gaagtcaagt ttgaaggtga taccttagtt aatagaatcg aattaaaagg tattgatttt
     6061 aaagaaggtg gtaacatttt aggtcacaaa ttggaataca actataactc tcacaatgtt
     6121 tacatcactg ctgacaaaca aaagaatggt atcaaagcta acttcaaaat tagacacaac
     6181 attgaagatg gtggtgttca attagctgac cattatcaac aaaatactcc aattggtgat
     6241 ggtccagtct tgttaccaga caaccattac ttatcctatc aatctgcctt atccaaagat
     6301 ccaaacgaaa agagagatca catggtcttg ttagaatttg ttactgctgc tggtattacc
     6361 catggtatgg atgaattgta caaaggatcc gggtcaggct ccggatctgg cccgccagcc
     6421 attcccccac gtcaaccaac gtaatggcag tgcttttaac taagaattat tagtcttttc
     6481 tgcttatttt ttcatcatag tttagaacac tttatattaa cgaatagttt atgaatctat
     6541 ttaggtttaa aaattgatac agttttataa gttacttttt caaagactcg tgctgtctat
     6601 tgcataatgc actggaaggg gaaaaaaaag gtgcacacgc gtggcttttt cttgaatttg
     6661 cagtttgaaa aatgctgagc agttacagag atgttacgaa ccactagtgc actgcagtac
     6721 atcgaggaga acttctagta tatctacata cctaatatta ttgccttatt aaaaatggaa
     6781 tcccaacaat tacatcaaaa tccacattct cttcaaaatc aattgtcctg tacttccttg
     6841 ttcatgtgtg ttcaaaaacg ttatatttat aggataatta tactctattt ctcaacaagt
     6901 aattggttgt ttggccgagc ggtctaaggc gcctgattca agaaatatct tgaccgcagt
     6961 taactgtggg aatactcagg tatcgtaaga tgcaagagtt cgaatctctt agcaaccatt
     7021 atttttttcc tcaacataac gagaacacac aggggcgcta tcgcacagaa tcaaattcga
     7081 tgactggaaa ttttttgtta atttcagagg tcgcctgacg catatacctt tttcaactga
     7141 aaaattggga gaaaaaggaa aggtgagagc gccggaaccg gcttttcata tagaatagag
     7201 aagcgttcat gactaaatgc ttgcatcaca atacttgaag ttgacaatat tatttaagga
     7261 cctattgttt tttccaatag gtggttagca atcgtcttac tttctaactt ttcttacctt
     7321 ttacatttca gcaatatata tatatatatt tcaaggatat accattctaa tgtctgcccc
     7381 taagaagatc gtcgttttgc caggtgacca cgttggtcaa gaaatcacag ccgaagccat
     7441 taaggttctt aaagctattt ctgatgttcg ttccaatgtc aagttcgatt tcgaaaatca
     7501 tttaattggt ggtgctgcta tcgatgctac aggtgttcca cttccagatg aggcgctgga
     7561 agcctccaag aaggctgatg ccgttttgtt aggtgctgtg ggtggtccta aatggggaac
     7621 cggtagtgtt agacctgaac aaggtttact aaaaatccgt aaagaacttc aattgtacgc
     7681 caacttaaga ccatgtaact ttgcatccga ctctctttta gacttatctc caatcaagcc
     7741 acaatttgct aaaggtactg acttcgttgt tgtcagagaa ttagtgggag gtatttactt
     7801 tggtaagaga aaggaagacg atggtgatgg tgtcgcttgg gatagtgaac aatacaccgt
     7861 tccagaagtg caaagaatca caagaatggc cgctttcatg gccctacaac atgagccacc
     7921 attgcctatt tggtccttgg ataaagctaa tgttttggcc tcttcaagat tatggagaaa
     7981 aactgtggag gaaaccatca agaacgaatt tcctacattg aaggttcaac atcaattgat
     8041 tgattctgcc gccatgatcc tagttaagaa cccaacccac ctaaatggta ttataatcac
     8101 cagcaacatg tttggtgata tcatctccga tgaagcctcc gttatcccag gttccttggg
     8161 tttgttgcca tctgcgtcct tggcctcttt gccagacaag aacaccgcat ttggtttgta
     8221 cgaaccatgc cacggttctg ctccagattt gccaaagaat aaggtcaacc ctatcgccac
     8281 tatcttgtct gctgcaatga tgttgaaatt gtcattgaac ttgcctgaag aaggtaaggc
     8341 cattgaagat gcagttaaaa aggttttgga tgcaggtatc agaactggtg atttaggtgg
     8401 ttccaacagt accaccgaag tcggtgatgc tgtcgccgaa gaagttaaga aaatccttgc
     8461 ttaaaaagat tctctttttt tatgatattt gtacataaac tttataaatg aaattcataa
     8521 tagaaacgac acgaaattac aaaatggaat atgttcatag ggtagacgaa actatatacg
     8581 caatctacat acatttatca agaaggagaa aaaggaggat gtaaaggaat acaggtaagc
     8641 aaattgatac taatggctca acgtgataag gaaaaagaat tgcactttaa cattaatatt
     8701 gacaaggagg agggcaccac acaaaaagtt aggtgtaaca gaaaatcatg aaactatgat
     8761 tcctaattta tatattggag gattttctct aaaaaaaaaa aaatacaaca aataaaaaac
     8821 actcaatgac ctgaccattt gatggagttt aagtcaatac cttcttgaac catttcccat
     8881 aatggtgaaa gttccctcaa gaattttact ctgtcagaaa cggccttaac gacgtagtcg
     8941 agagtactcg tatcgcatgt cggtgcgaca cgaaattaca aaatggaata tgttcatagg
     9001 gtagacgaaa ctatatacgc aatctacata catttatcaa gaaggagaaa aaggaggatg
     9061 taaaggaata caggtaagca aattgatact aatggctcaa cgtgataagg aaaaagaatt
     9121 gcactttaac attaatattg acaaggagga gggcaccaca caaaaagtta ggtgtaacag
     9181 aaaatcatga aactatgatt cctaatttat atattggagg attttctcta aaaaaaaaaa
     9241 aatacaacaa ataaaaaaca ctcaatgacc tgaccatttg atggagttta agtcaatacc
     9301 ttcttgaacc atttcccata atggtgaaag ttccctcaag aattttactc tgtcagaaac
     9361 ggccttaacg acgtagtcga cctcctcttc agtactaaat ctaccaatac caaatctgat
     9421 ggaagaatgg gctaatgcat catccttacc cagcgccgag cggccgcgtg ttacaaccaa
     9481 ttaaccaatt ctgattagaa aaactcatcg agcatcaaat gaaactgcaa tttattcata
     9541 tcaggattat caataccata tttttgaaaa agccgtttct gtaatgaagg agaaaactca
     9601 ccgaggcagt tccataggat ggcaagatcc tggtatcggt ctgcgattcc gactcgtcca
     9661 acatcaatac aacctattaa tttcccctcg tcaaaaataa ggttatcaag tgagaaatca
     9721 ccatgagtga cgactgaatc cggtgagaat ggcaaaagct tatgcatttc tttccagact
     9781 tgttcaacag gccagccatt acgctcgtca tcaaaatcac tcgcatcaac caaaccgtta
     9841 ttcattcgtg attgcgcctg agcgaggcga aatacgcgat cgctgttaaa aggacaatta
     9901 caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca gcgcatcaac aatattttca
     9961 cctgaatcag gatattcttc taatacctgg aatgctgttt tcccggggat cgcagtggtg
    10021 agtaaccatg catcatcagg agtacggata aaatgcttga tggtcggaag aggcataaat
    10081 tccgtcagcc agtttagtct gaccatctca tctgtaacat cattggcaac gctacctttg
    10141 ccatgtttca gaaacaactc tggcgcatcg ggcttcccat acaatcgata gattgtcgca
    10201 cctgattgcc cgacattatc gcgagcccat ttatacccat ataaatcagc atccatgttg
    10261 gaatttaatc gcggcctgga gcaagacgtt tcccgttgaa tatggctcat aacacccctt
    10321 gtattactgt ttatgtaagc agacagtttt attgttcatg atgatatatt tttatcttgt
    10381 gcaatgtaac atcagagatt ttgagacaca acgtggcttt gttgaataaa tcgaactttt
    10441 gctgagttga aggatcagtc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
    10501 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
    10561 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
    10621 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
    10681 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
    10741 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
    10801 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
    10861 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
    10921 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
    10981 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
    11041 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
    11101 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
    11161 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
    11221 gtgcggccgc caatacccag aagaacagta aaataaagca aggtacgtga aattaatatt
    11281 tttaaatggt tctaaccgat gccgaagaac tgcgcagtcc ggttataacg tctgacatgt
    11341 ccttttttga tttggaatcc aaccactcaa gtgactctgt tcatttactt tgcgaaaaat
    11401 atacccacaa attgcccatc gaaagtgaat cgcaaaccac cttcagactg gcaccgacaa
    11461 agcaaagatt atacagacag agtactttat acgtaccgtt aagtctcaag caaagggttt
    11521 tcttatttac tgaacgggta aagagtatct gggccggctt gccaagatgc aaaccgaata
    11581 agtatttcaa agttgcattt gccttagccg tcctgacacc attggctatt tggatatttt
    11641 atattgactt tcgtgtacat tgatcacatc gactgttcta ttggcaaatg aaccacgggc
    11701 attgactatt tttcaggtta ctactatata ttatcatcac gggcaaggat tgtaccctga
    11761 attcgcatct agactga
//
