LOCUS       sequence-476042-        4536 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4536)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4536)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC24A2_STOP
FEATURES             Location/Qualifiers
     source          1..4536
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(64..163)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(180..199)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(181..199)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(204..220)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(204..220)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(322..341)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             333..1142
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(400..419)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      1289..1877
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1778..1797
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2031..2048
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(2207..2234)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(2326..2412)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     2461..2483
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     2475..2492
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2476..2492
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2508..2607
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 tgtttctggt ggtgagcgtg ctgctggagg acagaatcct gacctgccca gtgtccatct
       61 aagacccagc tttcttgtac aaagttggca ttataagaaa gcattgctta tcaatttgtt
      121 gcaacgaaca ggtcactatc agtcaaaata aaatcattat ttgccatcca gctgatatcc
      181 cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc
      241 aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgaaca ataaaactgt
      301 ctgcttacat aaacagtaat acaaggggtg ttatgagcca tattcaacgg gaaacgtcga
      361 ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata
      421 atgtcgggca atcaggtgcg acaatctatc gcttgtatgg gaagcccgat gcgccagagt
      481 tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac
      541 taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg
      601 atgatgcatg gttactcacc actgcgatcc ccggaaaaac agcattccag gtattagaag
      661 aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc
      721 attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg
      781 cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg
      841 gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc tcaccggatt
      901 cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa
      961 taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc
     1021 tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg
     1081 gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct
     1141 aatcagaatt ggttaattgg ttgtaacact ggcagagcat tacgctgact tgacgggacg
     1201 gcgcaagctc atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag
     1261 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     1321 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     1381 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     1441 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     1501 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     1561 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     1621 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     1681 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     1741 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     1801 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     1861 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     1921 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     1981 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     2041 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     2101 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     2161 caattaatac gcgtaccgct agccaggaag agtttgtaga aacgcaaaaa ggccatccgt
     2221 caggatggcc ttctgcttag tttgatgcct ggcagtttat ggcgggcgtc ctgcccgcca
     2281 ccctccgggc cgttgcttca caacgttcaa atccgctccc ggcggatttg tcctactcag
     2341 gagagcgttc accgacaaac aacagataaa acgaaaggcc cagtcttccg actgagcctt
     2401 tcgttttatt tgatgcctgg cagttcccta ctctcgcgtt aacgctagca tggatgtttt
     2461 cccagtcacg acgttgtaaa acgacggcca gtcttaagct cgggccccaa ataatgattt
     2521 tattttgact gatagtgacc tgttcgttgc aacacattga tgagcaatgc ttttttataa
     2581 tgccaacttt gtacaaaaaa gcaggctcca ccatggacct gcagcagagc accacaatca
     2641 cctccctgga gaagtggtgc ctggatgagt ctctgagcgg ctgtcggaga cactacagcg
     2701 tgaagaagaa gctgaagctg atcagggtgc tgggcctgtt catgggcctg gtggccatct
     2761 ccaccgtgtc cttttctatc agcgccttct ccgagaccga cacacagtct acaggagagg
     2821 caagcgtggt gtccggacct agggtggcac agggatacca ccagcgcacc ctgctggacc
     2881 tgaatgataa gatcctggac tatacaccac agccccctct gtctaaggag ggcgagtctg
     2941 agaacagcac cgatcacgcc cagggcgact accccaagga tatcttttcc ctggaggaga
     3001 ggcgcaaggg cgccatcatc ctgcacgtga tcggcatgat ctatatgttc atcgccctgg
     3061 ccatcgtgtg cgatgagttc tttgtgcctt ccctgaccgt gatcacagag aagctgggca
     3121 tctctgacga tgtggcagga gccaccttta tggcagcagg aggcagcgcc ccagagctgt
     3181 ttacatccct gatcggcgtg ttcatcgccc actctaatgt gggaatcgga accatcgtgg
     3241 gaagcgccgt gttcaacatc ctgtttgtga tcggcatgtg cgccctgttc tctcgggaga
     3301 tcctgaatct gacatggtgg cccctgttta gagacgtgag cttctacatc gtggatctga
     3361 tcatgctgat catcttcttt ctggacaatg tgatcatgtg gtgggagagc ctgctgctgc
     3421 tgaccgccta cttctgttat gtggtgttca tgaagtttaa cgtgcaggtg gagaagtggg
     3481 tgaagcagat gatcaacagg aataaggtgg tgaaggtgac cgcaccagag gcacaggcaa
     3541 agccatccgc cgcaagggac aaggatgagc ctacactgcc agccaagcca aggctgcaga
     3601 ggggaggaag ctccgcctcc ctgcacaatt ctctgatgcg gaactccatc tttcagctga
     3661 tgatccacac actggaccca ctggccgagg agctgggctc ttatggcaag ctgaagtact
     3721 atgataccat gacagaggag ggccggttca gagagaaggc cagcatcctg cacaagatcg
     3781 ccaagaagaa gtgtcacgtg gatgagaacg agagacagaa tggcgccgcc aaccacgtgg
     3841 agaagatcga gctgcccaat tccacctcta cagacgtgga gatgaccccc tctagcgatg
     3901 ccagcgagcc tgtgcagaac ggcaatctgt cccacaacat cgagggagca gaggcacaga
     3961 cagcagacga ggaggaggat cagcctctgt ctctggcctg gccaagcgag acccggaagc
     4021 aggtgacatt cctgatcgtg tttcctatcg tgttcccact gtggatcacc ctgcccgacg
     4081 tgcggaagcc ttcctctaga aagttctttc ccatcacctt ctttggctcc atcacatgga
     4141 tcgccgtgtt ttcttatctg atggtgtggt gggcacacca agtgggagag accatcggca
     4201 tcagcgagga gatcatggga ctgaccatcc tggcagcagg cacatctatc cctgatctga
     4261 tcacaagcgt gatcgtggca aggaagggac tgggcgacat ggccgtgagc tcctctgtgg
     4321 gctccaacat cttcgatatc accgtgggac tgccactgcc atggctgctg tacacagtga
     4381 tccaccgctt tcagcctgtg gccgtgagct ccaatggcct gttctgcgcc atcgtgctgc
     4441 tgtttatcat gctgctgttc gtgatcctga gcatcgccct gtgcaagtgg cggatgaaca
     4501 agatcctggg cttcatcatg tttggcctgt atttcg
//
