LOCUS       sequence-47606-e         625 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses humanized ChR2(H134R)-mCherry in cells lacking Cre 
            (Cre-Off, flanked by alternative lox FAS sites), for optogenetic 
            excitation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Saunders A, Johnson CA, Sabatini BL
  TITLE     Novel recombinant adeno-associated viruses for Cre activated and 
            inactivated transgene expression in neurons.
  JOURNAL   Front Neural Circuits. 2012 Jul 27;6:47. doi: 
            10.3389/fncir.2012.00047. eCollection 2012.
  PUBMED    22866029
REFERENCE   2  (bases 1 to 625)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Neural Circuits."; date: "2012-07-27"; volume: "6:47. doi"; pages: "
            10.3389/fncir.2012.00047. eCollection 2012"
COMMENT     Sequence Label: pAAV-Ef1a-FAS-hChR2(H134R)-mCherry-WPRE-pA
FEATURES             Location/Qualifiers
     source          1..625
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      77..86
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gtgaggtacc ggatcctcta gagtcgactc cggaacaact tcgtatatac ctttctatac
       61 gaagttgtgg cgcgccgcca ccatggacta tggcggcgct ttgtctgccg tcggacgcga
      121 acttttgttc gttactaatc ctgtggtggt gaacgggtcc gtcctggtcc ctgaggatca
      181 atgttactgt gccggatgga ttgaatctcg cggcacgaac ggcgctcaga ccgcgtcaaa
      241 tgtcctgcag tggcttgcag caggattcag cattttgctg ctgatgttct atgcctacca
      301 aacctggaaa tctacatgcg gctgggagga gatctatgtg tgcgccattg aaatggttaa
      361 ggtgattctc gagttctttt ttgagtttaa gaatccctct atgctctacc ttgccacagg
      421 acaccgggtg cagtggctgc gctatgcaga gtggctgctc acttgtcctg tcatccttat
      481 ccgcctgagc aacctcaccg gcctgagcaa cgactacagc aggagaacca tgggactcct
      541 tgtctcagac atcgggacta tcgtgtgggg ggctaccagc gccatggcaa ccggctatgt
      601 taaagtcatc ttcttttgtc ttgga
//
