LOCUS       sequence-47607-e         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses humanized ChR2(H134R)-mCherry in cells lacking Cre 
            (Cre-Off, flanked by alternative lox FAS sites), for optogenetic 
            excitation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Saunders A, Johnson CA, Sabatini BL
  TITLE     Novel recombinant adeno-associated viruses for Cre activated and 
            inactivated transgene expression in neurons.
  JOURNAL   Front Neural Circuits. 2012 Jul 27;6:47. doi: 
            10.3389/fncir.2012.00047. eCollection 2012.
  PUBMED    22866029
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Neural Circuits."; date: "2012-07-27"; volume: "6:47. doi"; pages: "
            10.3389/fncir.2012.00047. eCollection 2012"
COMMENT     Sequence Label: pAAV-Ef1a-FAS-hChR2(H134R)-mCherry-WPRE-pA
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(90..797)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(368..387)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     637..655
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 caattttgta atccagaggt tgattatcga taagcttgat atcgaattca caacttcgta
       61 tagaaaggta tatacgaagt tgtgctagct tacttgtaca gctcgtccat gccgccggtg
      121 gagtggcggc cctcggcgcg ttcgtactgt tccacgatgg tgtagtcctc gttgtgggag
      181 gtgatgtcca acttgatgtt gacgttgtag gcgccgggca gctgcacggg cttcttggcc
      241 ttgtaggtgg tcttgacctc agcgtcgtag tggccgccgt ccttcagctt cagcctctgc
      301 ttgatctcgc ccttcagggc gccgtcctcg gggtacatcc gctcggagga ggcctcccag
      361 cccatggtct tcttctgcat tacggggccg tcggagggga agttggtgcc gcgcagcttc
      421 accttgtaga tgaactcgcc gtcctgcagg gaggagtcct gggtcacggt caccacgccg
      481 ccgtcctcga agttcatcac gcgctcccac ttgaagccct cggggaagga cagcttcaag
      541 tagtcgggga tgtcggcggg gtgcttcacg taggccttgg agccgtacat gaactgaggg
      601 gacaggatgt cccaggcgaa gggcaggggg ccacccttgg tcaccttcag cttggcggtc
      661 tgggtgccct cgtaggggcg gccctcgccc tcgccctcga tctcgaactc gtggccgttc
      721 acggagccct ccatgtgcac cttgaagcgc atgaactcct tgatgatggc catgttatcc
      781 tcctcgccct tgctcacggc ggccgctggc acggctccgg cctcggcttc gtcttcgacg
      841 agagtctcga cctcgatctc cgttccgccg atgttcagtt tggtggtttt gcggatatct
      901 ccgtgaatca atatgtgctc
//
