LOCUS       sequence-476144-        2301 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TMV 3'UTR Tobacco Mosaic Virus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Inckemann R, Chotel T, Brinkmann CK, Burgis M, Andreas L, Baumann J,
            Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Willmund 
            F, Mackinder LCM, Erb TJ
  TITLE     Advancing chloroplast synthetic biology through high-throughput 
            plastome engineering of Chlamydomonas reinhardtii
  JOURNAL   bioRxiv 2024.05.08.593163
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593163"
COMMENT     Sequence Label: pME_Cp_0_5_047_TMV_3'UTR_Tv
FEATURES             Location/Qualifiers
     source          1..2301
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      245..302
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(326..343)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(497..1085)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(577..596)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1268..1362)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1383..2042)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1957..1976
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     primer_bind     2204..2222
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2226..2269)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2272..2293
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 ggtctcggct taaataataa ataacggatt gtgtccgtaa tcacacgtgg tgcgtacgat
       61 aacgcatagt gtttttccct ccacttaaat cgaagggttg tgtcttggat cgcgcgggtc
      121 aaatgtatat ggttcatata catccgcagg cacgtaataa agcgaggggt tcgaatcccc
      181 ccgttacccc cggtaggggc ccacgcttga gaccactcat cgcgactagt agcggccgct
      241 gcagtccggc aaaaaagggc aaggtgtcac caccctgccc tttttcttta aaaccgaaaa
      301 gattacttcg cgttatgcag gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
      361 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
      421 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
      481 ccgcgttgct ggcgtttttc cacaggctcc gcccccctga cgagcatcac aaaaatcgac
      541 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
      601 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
      661 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
      721 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
      781 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
      841 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
      901 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
      961 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     1021 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     1081 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     1141 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     1201 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagctcga
     1261 ggcttggatt ctcaccaata aaaaacgccc ggcggcaacc gagcgttctg aacaaatcca
     1321 gatggagttc tgaggtcatt actggatcta tcaacaggag tccaagcgag ctcgatatca
     1381 aattacgccc cgccctgcca ctcgtcgcag tactgttgta attcattaag cattctgccg
     1441 acatggaagc catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg
     1501 tcgccttgcg tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg
     1561 gccacgttta aatcaaaact ggtgaaactc acccagggat tggctgatac gaaaaacata
     1621 ttctcaataa accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc
     1681 gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt cactccagag tgatgaaaac
     1741 gtttcagttt gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc
     1801 tcaccgtctt tcattgccat acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg
     1861 tgaataaagg ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta
     1921 atatccagct gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa
     1981 tgttctttac gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc
     2041 attttagctt ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat
     2101 cttatttcat tatggtgaaa gttggaacct cttacgtgcc cgatcaactc gagtgccacc
     2161 tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
     2221 gcagaatttc agataaaaaa aatccttagc tttcgctaag gatgatttct ggaattcgcg
     2281 gccgcttcta gagcgatgag t
//
