LOCUS       sequence-47641-e         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   White MD, Milne RV, Nolan MF
  TITLE     A Molecular Toolbox for Rapid Generation of Viral Vectors to Up- or 
            Down-Regulate Neuronal Gene Expression in vivo.
  JOURNAL   Front Mol Neurosci. 2011;4:8. Epub 2011 Jul 4.
  PUBMED    21772812
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Mol 
            Neurosci."; date: "2011"; volume: "4"; pages: "8. Epub 2011 Jul 4"
COMMENT     Sequence Label: pAAV-Gateway
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    24..612
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             130..141
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     539..559
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     protein_bind    634..757
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 ccgcccgggt cgacggtatc gatgcgggga ggcggcccaa agggagatcc gactcgtctg
       61 agggcgaagg cgaagacgcg gaagaggccg cagagccggc agcaggccgc gggaaggaag
      121 gtccgctgga ttgagggccg aagggacgta gcagaaggac gtcccgcgca gaatccaggt
      181 ggcaacacag gcgagcagcc aaggaaagga cgatgatttc cccgacaaca ccacggaatt
      241 gtcagtgccc aacagccgag cccctgtcca gcagcgggca aggcaggcgg cgatgagttc
      301 cgccgtggca atagggaggg ggaaagcgaa agtcccggaa aggagctgac aggtggtggc
      361 aatgccccaa ccagtggggg ttgcgtcagc aaacacagtg cacaccacgc cacgttgcct
      421 gacaacgggc cacaactcct cataaagaga cagcaaccag gatttataca aggaggagaa
      481 aatgaaagcc atacgggaag caatagcatg atacaaaggc attaaagcag cgtatccaca
      541 tagcgtaaaa ggagcaacat agttaagaat accagtcaat ctttcacaaa ttttgtaatc
      601 cagaggttga ttatcgataa gcttgatatc tagccacttt gtacaagaaa gctgaacgag
      661 aaacgtaaaa tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta
      721 cataatactg taaaacacaa catatccagt cactatggtc gacctgcaga ctggctgtgt
      781 ataagggagc ctgacattta tattccccag aacatcaggt taatggcgtt tttgatgtca
      841 ttttcgcgtg ctga
//
