LOCUS       sequence-47648-e         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Knobel PA, Kotov IN, Felley-Bosco E, Stahel RA, Marti TM
  TITLE     Inhibition of REV3 expression induces persistent DNA damage and 
            growth arrest in cancer cells.
  JOURNAL   Neoplasia. 2011 Oct;13(10):961-70.
  PUBMED    22028621
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Neoplasia."; date: "2011-10"; volume: "13(10)"; pages: "961-70"
COMMENT     Sequence Label: pSUPERIOR.puro-shREV3-6
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     17..33
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     18..34
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    100..118
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(164..183)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     promoter        329..828
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(364..387)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     761..780
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 ggtaccgggc cccccctcga ggtcgacggt atcgataagc ttaaaaatag tcagactttt
       61 cagccttctc ttgaaaggct gaaaagtctg actaggggat ctctatcact gatagggaac
      121 ttataagatt cccaaatcca aagacatttc acgtttatgg tgatttccca gaacacatag
      181 cgacatgcaa atattgcagg gcgccactcc cctgtccctc acagccatct tcctgccagg
      241 gcgcacgcgc gctgggtgtt cccgcctagt gacactgggc ccgcgattcc ttggagcggg
      301 ttgatgacgt cagcgttcga attctaccgg gtaggggagg cgcttttccc aaggcagtct
      361 ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct
      421 cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc
      481 gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg
      541 tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc
      601 gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc
      661 gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc
      721 aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag
      781 cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcagccca
      841 agctagctta
//
