LOCUS       sequence-476572-        2904 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  express aeBlue chromoprotein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Collins M, Lau MB, Ma W, Shen A, Wang B, Cai S, La Russa M, Jewett 
            MC, Qi LS
  TITLE     A frugal CRISPR kit for equitable and accessible education in gene 
            editing and synthetic biology.
  JOURNAL   Nat Commun. 2024 Aug 3;15(1):6563. doi: 10.1038/s41467-024-50767-2.
  PUBMED    39095367
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-08-3"; volume: "15(1):6563. doi"; pages: " 
            10.1038/s41467-024-50767-2"
COMMENT     Sequence Label: pSLQ14101 aeBlue
FEATURES             Location/Qualifiers
     source          1..2904
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(747..774)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(790..861)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     RBS             1593..1604
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     promoter        complement(1620..1654)
                     /label=J23119(SpeI) promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119) modified to end with an SpeI site"
     promoter        1728..1832
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1833..1901,1902..2693)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2051..2070)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     terminator      2723..2817
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgccctagg tataaacgca gaaaggccca cccgaaggtg agccagtgtg
      781 actctagtag agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtctttcga
      841 ctgagccttt cgttttattt gatgcctgga gatccttact cgagttatta ttagtgatgc
      901 cctaatttac tcggtgccgc ctcacagtaa cgggccaccg ctgactcgta ttgttcataa
      961 aatttacctt gttcagcttt caaaatctca atgcgatgat ccccaaaatg aaattccggc
     1021 atgttaactg cattggatgg cttgcgagaa cgataggtag tgcgcaggtg ggacgtcaga
     1081 tgattgccat cagtgcattt cagggccatc aggctctgac cacacaggac gccgtcccgc
     1141 ggataaagca tctcaacgca cggttcccat ccagctgttt ttttctgcat cacgggaccg
     1201 tttgcaggga agttggcacc gatgacatta actttgaaaa taaaattatt accctgaagg
     1261 ctcgtgtcct ggtgtatggt gagatagccg ccatcctcga agatttgtgt tctttcccag
     1321 gtaaagccct caggaaaaga ctccttaaag taatccggaa tacccgacac atgcttaatg
     1381 aaggttttac tgccatacat gcaacaaggg gccaaaatat catacgcaaa tggtaagggc
     1441 ccaccttcag tgatgcgtat cttttccacc tgggtccctt caaatggttt gccttcgcct
     1501 tctcctacac acttgaaatg gtgaccgttt actgttcctt ccatcgtcat tttgatgcac
     1561 atgtcttttt taaccagtga agccatagat cctttctcct ctttagatct tttgaattca
     1621 ctagtattat acctaggact gagctagctg tcaagcgcaa cgcaattaat gtaagttagc
     1681 tcactcatta ggcaccgacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     1741 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     1801 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     1861 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     1921 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     1981 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     2041 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     2101 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     2161 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     2221 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     2281 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     2341 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     2401 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     2461 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     2521 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     2581 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     2641 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     2701 accaagttta cgagctcgct tggactcctg ttgatagatc cagtaatgac ctcagaactc
     2761 catctggatt tgttcagaac gctcggttgc cgccgggcgt tttttattgg tgagaatcca
     2821 agcactagtc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     2881 cgtagaaaag atcaaaggat cttc
//
