LOCUS       sequence-476799-       15976 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express IFIH1-HiBiT in Mammalian Cells under a CMV promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15976)
  AUTHORS   Promega
  TITLE     Promega Plasmid Collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: IFIH1-HiBiT Fusion Vector
FEATURES             Location/Qualifiers
     source          1..15976
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    828..876
                     /note="synthetic polyadenylation signal"
     CDS             complement(940..1734)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(1766..1813)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(1828..2185)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1842..1977
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        1994..2185
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     2142..2162
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     polyA_signal    2430..2551
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     2521..2540
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2588..2606
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(5859..6371)
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     promoter        complement(6400..6418)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6401..6418)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     enhancer        6528..6831
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7039..7086
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7118..7777
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(7184..7203)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      complement(7949..8537)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     polyA_signal    8663..8711
                     /note="synthetic polyadenylation signal"
     CDS             complement(8735..9529)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(9907..11085)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          9916..10854
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     complement(9939..9959)
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     polyA_signal    11102..11183
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(14473..14492)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(14474..14492)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(14534..14553)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          14536..14668
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        complement(14804..15007)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        15008..15387
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    15578..15861
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
ORIGIN
        1 ttgaaatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgggta
      721 gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat gtcagctact gggctatctg
      781 gacaagggaa aacgcaagcg caaagaggat ccttatcgct atcgattcac acaaaaaacc
      841 aacacacaga tgtaatgaaa ataaagatat tttattgcgg ccatcgtgat ggcaggttgg
      901 gcgtcgcttg gtcggtcatt tcgaacccca gagtcccgct cagaagaact cgtcaagaag
      961 gcgatagaag gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg
     1021 gtcagcccat tcgccgccaa gctcttcagc aatatcacgg gtagccaacg ctatgtcctg
     1081 atagcggtcc gccacaccca gccggccaca gtcgatgaat ccagaaaagc ggccattttc
     1141 caccatgata ttcggcaagc aggcatcgcc atgggtcacg acgagatcct cgccgtcggg
     1201 catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg agcccctgat gctcttcgtc
     1261 cagatcatcc tgatcgacaa gaccggcttc catccgagta cgtgctcgct cgatgcgatg
     1321 tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc
     1381 atcagccatg atggatactt tctcggcagg agcaaggtga gatgacagga gatcctgccc
     1441 cggcacttcg cccaatagca gccagtccct tcccgcttca gtgacaacgt cgagcacagc
     1501 tgcgcaagga acgcccgtcg tggccagcca cgatagccgc gctgcctcgt cctgcagttc
     1561 attcagggca ccggacaggt cggtcttgac aaaaagaacc gggcgcccct gcgctgacag
     1621 ccggaacacg gcggcatcag agcagccgat tgtctgttgt gcccagtcat agccgaatag
     1681 cctctccacc caagcggccg gagaacctgc gtgcaatcca tcttgttcaa tcatgatctg
     1741 cctcctgggt ttagttcctc accttgtcgt attatactat gccgatatac tatgccgatg
     1801 attaattgtc aacagttaat taagcttttt gcaaaagcct aggcctccaa aaaagcctcc
     1861 tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat aaataaaaaa
     1921 aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg gcgggatggg
     1981 cggagttagg ggcgggacta tggttgctga ctaattgaga tgcatgcttt gcatacttct
     2041 gcctgctggg gagcctgggg actttccaca cctggttgct gactaattga gatgcatgct
     2101 ttgcatactt ctgcctgctg gggagcctgg ggactttcca caccctaact gacacacatt
     2161 ccacagctgg ttctttccgc ctcagaaggt acctaaccaa gttcctcttt cagaggttat
     2221 ttcaggccat ggtgctgcgc agatccgcgt atgcggtgtg aaataccgca cagatgcgta
     2281 aggagaaaat accgcatcag gaaattgtaa gcgttaatat tttgttaaaa ttcgatttta
     2341 ccacatttgt agaggtttta cttgcttaaa aaaacctccc acatctcccc ctgaacctga
     2401 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
     2461 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     2521 gtggtttgtc caaactcatc aatgtatctt atcatgtctg ctcgaagcgg ccgccccaag
     2581 gggttatgct agttattgct cagcggtggc agcagccaac tcagcttcct ttcgggcttt
     2641 gttagcagcc ggatcagctt gcatgcctgc aggtcgactc tagagtcgcg gccttagcta
     2701 atcttcttga acagccgcca gccgctcaca ccgctcgagc cacctgaact gccttgagaa
     2761 acatcctcat cactaaataa acagcattct gaatagtcaa gattgggaaa tgtgataggt
     2821 aattctaccc actttttgta ttgtttcttt gttgaattat ttttgaaaac cactacaaaa
     2881 ttccttattt tgagacaagg caaatctaag cctttgtgca ccatcattgt tccccaagcc
     2941 tggccacatt tgcagatgat ttcaccattt atttgatagt cggcacactt cttttgcagt
     3001 gctttgtttt ctcttacaat gtaaagttcc ttgaattctg gggtcatatt gacgtgatgc
     3061 attttctcaa ttacatggat atcttcccca gaacaggcta gcacactgca gtttttgcaa
     3121 aggaaagtta ttagtgatgg gttattcttg taatgcttgg caatatttct cttggttttc
     3181 attttctttt ccattatact ttgcatctgt aattccaaaa tcttatgagc atactcctct
     3241 ggtttcatat tttgaacaca atgtatagct ttatacatca tcttctctcg gaaatcatta
     3301 actgtctcat gttcgataac tcctgaacca ctgtgagcaa ccaggacgta ggtgctctca
     3361 tcagctctgg ctcgaccacg ggcctggacc atggctattt cattggtgac gagaccataa
     3421 cggataacaa tgttacattc tttaatatcc agaccttctt ctgccactgt ggtagcgata
     3481 agcagattta tttttccagt gcgaaattta ctaatgactt ctttttgttc attctgtgtc
     3541 atgggtttga actcactgct gtgtccagct ccaatcagat ggtgggcttt gactcctact
     3601 tcagcaaatt tttcattttc agtaatccac tgggaaagcg catatgcact ctgtcgtgtt
     3661 tttgtaaaga ttattcctcg tgctgattcc tcagtcctag tatattgctc cattatggta
     3721 tttcttaatt tggtcagctt ttcattttca tattctgggt tttcagccag ccttttcaac
     3781 attttattgt tttcaaaaaa taaagtcatg agaaatctat ctgtttcatc cagtttcaaa
     3841 ggtttcttta aatcatcctc atcttcatca ccatcacaat actcatcatc accaccctca
     3901 tcactatcat cttctatgac tgcaaacttc ttatctttct cttcattata gaaagtttca
     3961 agatgagtat acgcatctat cattcgaatt gtgtcattaa tttgtagggc ctcattgtac
     4021 ttcctcaaat gttctgcaca aacacgttct ttgcgatttc cttcttttgc agcttttttt
     4081 tccatttgaa tggcccattg ttcatagggt tgagttccaa aatctgacat tggactcatt
     4141 tgacaataag tttgaatcct tgtcattatt tctagaagtt tctctttaaa tggatcttct
     4201 ctggttgcat ctgcaatggc aaacttcttg catggctcct gtatttggtt tttcagttga
     4261 tcaaggtttt ctttaacagt tttaatagta aatgcatcaa gattggcaca tagttttaaa
     4321 atgtgttctt cagctttggc ttgcttcgtg gcccctccaa caccaggtga agctgttagt
     4381 cccagtatct gaggaagggg aatcactggt ttgttttctt tcttgagtct attgtttttc
     4441 aacttctgca tcaaataatg cctcatgatg ttattataca ctgcttcttt gttggtgtga
     4501 tgacattcat caatgataat gagggaaaag tctgacaatt gaacaccagc atcttctcca
     4561 ttttccaagt ttaagaggga gttttcaagg atttgagctg tactgataat aatatcacag
     4621 gacttgacaa cttctggaaa tgatattttc agttgggtat caccacttaa tccaataaca
     4681 cgataccatt tcttcaaaaa tggttggaac tccttgcgga agagctgttc aactagcagt
     4741 accttattga caagaactat aacttttcca ggctcagatg cttttttctt cttgtctaag
     4801 tgatccttgg caatgtaaac agccactctg gtttttccac tccctgtagg gaggcagatg
     4861 atgatattct tcccttccaa ggctggctgg gcaacttcca tttggtaagg cctgagctgg
     4921 agttctggct ccggggatgc tcttgctgcc acattctctt catctgaatc acttcccatg
     4981 gtgcctgaat cactgcccat gttgctgtta tgtccaagac tttcatctaa gcagctgaca
     5041 cttccttctg ccaaacttgt gtctgattct gaaactacag aagaatctgc aaaagatgat
     5101 tctgatgagt tattctccat gccccagacc tccttctcca gatttggctg aactgtggtt
     5161 gaaagaagtt gctcttccac ttgaggacca tcaacttgtg ataaattctc aatctctgca
     5221 ttgctttctg agcaatcaga gcctgttaac tcttggacaa gttcattgtt tcctgtttga
     5281 cgaagaacat tcagaaatgc agagaaccag ttttctttct gcacaatcct ttttagtagc
     5341 tctcttacac ctgattcatt tccattgttt tctgcagcag caatccggtt tctgtcttca
     5401 attgtcaaca gttcctcctc catgcactta tccaagacgt ctctaactag aagcttgtcc
     5461 accagagtgg gctgaaggag gttcagcagt tggagatatt catcatgagc gttctcaaac
     5521 gatggagagg gcaagtccgt gagctcaggg ttcatgtagc gggcggccag agggctgccg
     5581 gttctccgga gggcctccac gaattcccga gtccaaccaa ggtgccagac tcccttctcc
     5641 aaggtgctca gcagcagttc aactgcctgc atgttcccgg aggtggcgac tgtcctctga
     5701 atctgctcct tcacctctgc aggcagaaag gtcaggtagt ccagcacagg ctccacctgg
     5761 atgtacattt tcaccctggc cctgaagcac gagatgagat agcggaaatt ctcgtctgtg
     5821 gaatacccat tcgacatggc gatcgcgccg ctcgagcccg ccagaatgcg ttcgcacagc
     5881 cgccagccgg tcactccgtt gatggttact cggaacagca gggagccgtc ggggttgatc
     5941 aggcgctcgt cgataatttt gttgccgttc cacagggtcc ctgttacagt gatctttttg
     6001 ccgtcgaaca cggcgatgcc ttcatacggc cgtccgaaat agtcgatcat gttcggcgta
     6061 accccgtcga ttaccagtgt gccatagtgc aggatcacct taaagtgatg atcatccaca
     6121 gggtacacca ccttaaaaat tttttcgatc tggcccattt ggtcgccgct cagaccttca
     6181 tacgggatga tgacatggat gtcgatcttc agcccatttt caccgctcag gacaatcctt
     6241 tggatcggag ttacggacac cccgagattc tgaaacaaac tggacacacc tccctgttca
     6301 aggacttggt ccaggttgta gccggctgtc tgtcgccagt ccccaacgaa atcttcgagt
     6361 gtgaagacca tggttgcaaa aagaacaagt agcttgtatt ctatagtgtc acctaaatgg
     6421 gcgatctgac ggttcactaa accagctctg cttataaggc gcgccactag ttattaatag
     6481 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
     6541 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
     6601 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
     6661 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tccgccccct
     6721 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttacgg
     6781 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
     6841 ttttggcagt acaccaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
     6901 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
     6961 tgtcgtaata accccgcccc gttgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
     7021 aaatcgaatt ctaatactgt tgacaattaa tcatcggcat agtatatcgg catagtataa
     7081 tacgacaagg tgaggaacta aacccaggag gcagatcatg gagaaaaaaa tcactggata
     7141 taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt
     7201 tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt
     7261 aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa
     7321 tgctcatccg gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt
     7381 tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga
     7441 ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg
     7501 tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg tctcagccaa
     7561 tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc
     7621 ccccgttttc actatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc
     7681 gattcaggtt catcatgccg tctgtgatgg cttccatgtc ggcagaatgc ttaatgaatt
     7741 acaacagtac tgcgatgagt ggcagggcgg ggcgtaagcg ggactctggg gttcgaaatg
     7801 accgaccaag cgacgcccaa ccggtatcag ctcactcaaa ggcggtaata cggttatcca
     7861 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     7921 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     7981 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     8041 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     8101 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     8161 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     8221 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     8281 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     8341 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     8401 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     8461 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     8521 gaaaaaaagg atttcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     8581 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     8641 tccttttata gtccggaatc gtcacacaaa aaaccaacac acagatgtaa tgaaaataaa
     8701 gatattttat tgcggccatc gtgatggcta gagttcagaa gaactcgtca agaaggcgat
     8761 agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg aagcggtcag
     8821 cccattcgcc gccaagctct tcagcaatat cacgggtagc caacgctatg tcctgatagc
     8881 ggtccgccac acccagccgg ccacagtcga tgaatccaga aaagcggcca ttttccacca
     8941 tgatattcgg caagcaggca tcgccgtggg tcacgacgag atcctcgccg tcgggcatgc
     9001 gcgccttgag cctggcgaac agttcggctg gcgcgagccc ctgatgctct tcgtccagat
     9061 catcctgatc gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg
     9121 cttggtggtc gaatgggcag gtagccggat caagcgtatg cagccgccgc attgcatcag
     9181 ccatgatgga tactttctcg gcaggagcaa ggtgagatga caggagatcc tgccccggca
     9241 cttcgcccaa tagcagccag tcccttcccg cttcagtgac aacgtcgagc acagctgcgc
     9301 aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc ctcgtcctgc agttcattca
     9361 gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga
     9421 acacggcggc atcagagcag ccgattgtct gttgtgccca gtcatagccg aatagcctct
     9481 ccacccaagc ggccggagaa cctgcgtgca atccatcttg ttcaatcatg gtggcatcga
     9541 ttcccggtca acatccagta cacctccggc acaccggctt ccccaagggc gccacgctct
     9601 cccaccacaa catcctcaac aacggctact gggtgggccg cacggtcggc tacacggagc
     9661 aggaccgggt gtgtctgccg gtgcccttct accactgctt cggcatggtc atgggcaact
     9721 tgggcgccac ctcccacgcc gcctgcatcg tcatcccggc cccgtccttc gagccggcgg
     9781 cgacgctgga ggcggtgcag cgggagaggt gcacgtccct gtacggcgtc cccacgaagc
     9841 cgaattccac cacactggac tagatgcatg ctcgacagtg tgatggatat ctgcagaatt
     9901 cggctttcac gacacctgaa atggaagaaa aaaactttga accactgtct gaggcttgag
     9961 aatgaaccaa gatccaaact caaaaagggc aaattccaag gagaattaca tcaagtgcca
    10021 agctggccta acttcagtct ccacccactc agtgtgggga aactccatcg cataaaaccc
    10081 ctccccccaa cctaaagacg acgtactcca aaagctcgag aactaatcga ggtgcctgga
    10141 cggcgcccgg tactccgtgg agtcacatga agcgacggct gaggacggaa aggccctttt
    10201 cctttgtgtg ggtgactcac ccgcccgctc tcccgagcgc cgcgtcctcc attttgagct
    10261 gcctgcagca gggccgggaa gcggccatct ttccgctcac gcaactggtg ccgaccgggc
    10321 cagccttgcc gcccagggcg gggcgataca cggcggcgcg aggccaggca ccagagcagg
    10381 ccggccagct tgagactacc cccgtccgat tctcggtggc cgcgctcgca ggccccgcct
    10441 cgccgaacat gtgcgctggg acgcacgggc cccgtcgccg cccgcggccc caaaaaccga
    10501 aataccagtg tgcagatctt ggcccgcatt tacaagacta tcttgccaga aaaaaagcgt
    10561 cgcagcaggt catcaaaaat tttaaatggc tagagactta tcgaaagcag cgagacaggc
    10621 gcgaaggtgc caccagattc gcacgcggcg gccccagcgc ccaggccagg cctcaactca
    10681 agcacgaggc gaaggggctc cttaagcgca aggcctcgaa ctctcccacc cacttccaac
    10741 ccgaagctcg ggatcaagaa tcacgtactg cagccaggtg gaagtaattc aaggcacgca
    10801 agggccataa cccgtaaaga ggccaggccc gcgggaacca cacacggcac ttacctgtgt
    10861 tctggcggca aacccgttgc gaaaaagaac gttcacggcg actactgcac ttatatacgg
    10921 ttctccccca ccctcgggaa aaaggcggag ccagtacacg acatcacttt cccagtttac
    10981 cccgcgccac cttctctagg caccggttca attgccgacc cctcccccca acttctcggg
    11041 gactgtgggc gatgtgcgct ctgcccactg acgggcaccg gagccaagac gcgtgacgtc
    11101 ggcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
    11161 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
    11221 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
    11281 attatgatcc gcggccgcaa tacgtcgacg ttatcagctg acttcgtacg agagcctagg
    11341 tgtttctaga ggatccccgg gtaccgagcc cgaattcgtt taaacatcct catcactaaa
    11401 taaacagcat tctgaatagt caagattggg aaatgtgata ggtaattcta cccacttttt
    11461 gtattgtttc tttgttgaat tatttttgaa aaccactaca aaattcctta ttttgagaca
    11521 aggcaaatct aagcctttgt gcaccatcat tgttccccaa gcctggccac atttgcagat
    11581 gatttcacca tttatttgat agtcggcaca cttcttttgc agtgctttgt tttctcttac
    11641 aatgtaaagt tccttgaatt ctggggtcat attgacgtga tgcattttct caattacatg
    11701 gatatcttcc ccagaacagg ctagcacact gcagtttttg caaaggaaag ttattagtga
    11761 tgggttattc ttgtaatgct tggcaatatt tctcttggtt ttcattttct tttccattat
    11821 actttgcatc tgtaattcca aaatcttatg agcatactcc tctggtttca tattttgaac
    11881 acaatgtata gctttataca tcatcttctc tcggaaatca ttaactgtct catgttcgat
    11941 aactcctgaa ccactgtgag caaccaggac gtaggtgctc tcatcagctc tggctcgacc
    12001 acgggcctgg accatggcta tttcattggt gacgagacca taacggataa caatgttaca
    12061 ttctttaata tccagacctt cttctgccac tgtggtagcg ataagcagat ttatttttcc
    12121 agtgcgaaat ttactaatga cttctttttg ttcattctgt gtcatgggtt tgaactcact
    12181 gctgtgtcca gctccaatca gatggtgggc tttgactcct acttcagcaa atttttcatt
    12241 ttcagtaatc cactgggaaa gcgcatatgc actctgtcgt gtttttgtaa agattattcc
    12301 tcgtgctgat tcctcagtcc tagtatattg ctccattatg gtatttctta atttggtcag
    12361 cttttcattt tcatattctg ggttttcagc cagccttttc aacattttat tgttttcaaa
    12421 aaataaagtc atgagaaatc tatctgtttc atccagtttc aaaggtttct ttaaatcatc
    12481 ctcatcttca tcaccatcac aatactcatc atcaccaccc tcatcactat catcttctat
    12541 gactgcaaac ttcttatctt tctcttcatt atagaaagtt tcaagatgag tatacgcatc
    12601 tatcattcga attgtgtcat taatttgtag ggcctcattg tacttcctca aatgttctgc
    12661 acaaacacgt tctttgcgat ttccttcttt tgcagctttt ttttccattt gaatggccca
    12721 ttgttcatag ggttgagttc caaaatctga cattggactc atttgacaat aagtttgaat
    12781 ccttgtcatt atttctagaa gtttctcttt aaatggatct tctctggttg catctgcaat
    12841 ggcaaacttc ttgcatggct cctgtatttg gtttttcagt tgatcaaggt tttctttaac
    12901 agttttaata gtaaatgcat caagattggc acatagtttt aaaatgtgtt cttcagcttt
    12961 ggcttgcttc gtggcccctc caacaccagg tgaagctgtt agtcccagta tctgaggaag
    13021 gggaatcact ggtttgtttt ctttcttgag tctattgttt ttcaacttct gcatcaaata
    13081 atgcctcatg atgttattat acactgcttc tttgttggtg tgatgacatt catcaatgat
    13141 aatgagggaa aagtctgaca attgaacacc agcatcttct ccattttcca agtttaagag
    13201 ggagttttca aggatttgag ctgtactgat aataatatca caggacttga caacttctgg
    13261 aaatgatatt ttcagttggg tatcaccact taatccaata acacgatacc atttcttcaa
    13321 aaatggttgg aactccttgc ggaagagctg ttcaactagc agtaccttat tgacaagaac
    13381 tataactttt ccaggctcag atgctttttt cttcttgtct aagtgatcct tggcaatgta
    13441 aacagccact ctggtttttc cactccctgt agggaggcag atgatgatat tcttcccttc
    13501 caaggctggc tgggcaactt ccatttggta aggcctgagc tggagttctg gctccgggga
    13561 tgctcttgct gccacattct cttcatctga atcacttccc atggtgcctg aatcactgcc
    13621 catgttgctg ttatgtccaa gactttcatc taagcagctg acacttcctt ctgccaaact
    13681 tgtgtctgat tctgaaacta cagaagaatc tgcaaaagat gattctgatg agttattctc
    13741 catgccccag acctccttct ccagatttgg ctgaactgtg gttgaaagaa gttgctcttc
    13801 cacttgagga ccatcaactt gtgataaatt ctcaatctct gcattgcttt ctgagcaatc
    13861 agagcctgtt aactcttgga caagttcatt gtttcctgtt tgacgaagaa cattcagaaa
    13921 tgcagagaac cagttttctt tctgcacaat cctttttagt agctctctta cacctgattc
    13981 atttccattg ttttctgcag cagcaatccg gtttctgtct tcaattgtca acagttcctc
    14041 ctccatgcac ttatccaaga cgtctctaac tagaagcttg tccaccagag tgggctgaag
    14101 gaggttcagc agttggagat attcatcatg agcgttctca aacgatggag agggcaagtc
    14161 cgtgagctca gggttcatgt agcgggcggc cagagggctg ccggttctcc ggagggcctc
    14221 cacgaattcc cgagtccaac caaggtgcca gactcccttc tccaaggtgc tcagcagcag
    14281 ttcaactgcc tgcatgttcc cggaggtggc gactgtcctc tgaatctgct ccttcacctc
    14341 tgcaggcaga aaggtcaggt agtccagcac aggctccacc tggatgtaca ttttcaccct
    14401 ggccctgaag cacgagatga gatagcggaa attctcgtct gtggaatacc cattcgacat
    14461 ggcgatcgct agccctatag tgagtcgtat taatactcta gccttaagag ctgtaattga
    14521 actgggagtg gacacctgtg gagagaaagg caaagtggat gtcagtaaga ccaataggtg
    14581 cctatcagaa acgcaagagt cttctctgtc tcgacaagcc cagtttctat tggtctcctt
    14641 aaacctgtct tgtaaccttg atacttacct gcccagtgcc tcacgaccaa cttctgcagc
    14701 ttaagttcga gactgttgtg tcagaagcac tgactgcgtt agcaatttaa ctgtgataaa
    14761 ctaccgcaat aaagcttcta gtgatctgac ggttcactaa accagctctg cttatataga
    14821 cctcccaccg tacacgccta ccgcccattt gcgtcaacgg ggcggggtta ttacgacatt
    14881 ttggaaagtc ccgttgattt tggtgccaaa acaaactccc attgacgtca atggggtgga
    14941 gacttggaaa tccccgtgag tcaaaccgct atccacgccc attggtgtac tgccaaaacc
    15001 gcatcaccat ggtaatagcg atgactaata cgtagatgta ctgccaagta ggaaagtccc
    15061 gtaaggtcat gtactgggca taatgccagg cgggccattt accgtcattg acgtcaatag
    15121 ggggcggact tggcatatga tacacttgat gtactgccaa gtgggcagtt taccgtaaat
    15181 actccaccca ttgacgtcaa tggaaagtcc ctattggcgt tactatggga acatacgtca
    15241 ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca tttaccgtaa
    15301 gttatgtaac gcggaactcc atatatgggc tatgaactaa tgaccccgta attgattact
    15361 attaataact agtcaataat caatgccaac atggcggtca tattggacat gagccaatat
    15421 aaatgtacat attatgatat agatacaacg tatgcaatgg ccaatagcca atattgattt
    15481 atgctatata accaatgaat aatatggcta atggccaata ttgaagatct cgatcctcta
    15541 cgccggacgc atcgtggccg gagtactgcg gtttatctcg atggctacga gggcagacag
    15601 taagtggatt taccataatc ccttaattgt acgcaccgct aaaacgcgtt cagcgcgatc
    15661 acggcagcag acaggtaaaa atggcaacaa accacccgaa aaactgccgc aatcgcgcct
    15721 gataaatttt aaccgtatga atacctatgc aaccagaggg tacaggccac attaccccca
    15781 cttaatccac tgaagctgcc atttttcatg gtttcaccat cccagcgaag ggccatccag
    15841 cgtgcgttcc tgtatttccg gactataaaa ggatctaggt gaagatcctt tttgataatc
    15901 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
    15961 agatcaaagg atcttc
//
