LOCUS       sequence-47689-e         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Bakota L, Brandt R, Heinisch JJ
  TITLE     Triple mammalian/yeast/bacterial shuttle vectors for single and 
            combined Lentivirus- and Sindbis virus-mediated infections of 
            neurons.
  JOURNAL   Mol Genet Genomics. 2012 Apr;287(4):313-24. Epub 2012 Feb 12.
  PUBMED    22327349
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Genet 
            Genomics."; date: "2012-04"; volume: "287(4)"; pages: "313-24. Epub 
            2012 Feb 12"
COMMENT     Sequence Label: pJJH1308
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(302..335)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        403..746
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 ttgacgtcaa tggaaagtcc ctattggcgt tactatggga acatacgtca ttattgacgt
       61 caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca tttaccgtaa gttatgtaac
      121 gcggaactcc atatatgggc tatgaactaa tgaccccgta attgattact attaataact
      181 agtcaataat caatgtcaac gcgtatatct ggcccgtaca tcgcgaagca gcgcaaaacg
      241 cctaacccta agcagattct tcatgcaatg cataggccac tagtggatct gatatcacct
      301 aataacttcg tatagcatac attatacgaa gttatattaa gggttctcga gaagcttgga
      361 tctgtttagc ttgcctcgtc cccgccgggt cacccggcca gcgacatgga ggcccagaat
      421 accctccttg acagtcttga cgtgcgcagc tcaggggcat gatgtgactg tcgcccgtac
      481 atttagccca tacatcccca tgtataatca tttgcatcca tacattttga tggccgcacg
      541 gcgcgaagca aaaattacgg ctcctcgctg cagacctgcg agcagggaaa cgctcccctc
      601 acagacgcgt tgaattgtcc ccacgccgcg cccctgtaga gaaatataaa aggttaggat
      661 ttgccactga ggttcttctt tcatatactt ccttttaaaa tcttgctagg atacagttct
      721 cacatcacat ccgaacataa acaaccatgt ccacaaaatc atataccagt agagctgaga
      781 ctcatgcaag tccggttgca tcgaaacttt tacgtttaat g
//
