LOCUS       sequence-47698-e         807 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 807)
  AUTHORS   Young CL, Raden DL, Caplan JL, Czymmek KJ, Robinson AS
  TITLE     Cassette series designed for live-cell imaging of proteins and 
            high-resolution techniques in yeast.
  JOURNAL   Yeast. 2012 Mar;29(3-4):119-36. doi: 10.1002/yea.2895. Epub 2012 Apr
            4.
  PUBMED    22473760
REFERENCE   2  (bases 1 to 807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "2012-03"; volume: "29(3-4):119-36. doi"; pages: " 
            10.1002/yea.2895. Epub 2012 Apr 4"
COMMENT     Sequence Label: pCY 3080-07
FEATURES             Location/Qualifiers
     source          1..807
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(59..255)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
     CDS             complement(261..635)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
ORIGIN
        1 atctgatatc atcgatgaat tcgagctcgg gatggcggcg ttagtatcga atcgacagca
       61 gtatagcgac cagcattcac atacgattga cgcatgatat tactttctgc gcacttaact
      121 tcgcatctgg gcagatgatg tcgaggcgaa aaaaatataa atcacgctaa catttgatta
      181 aaatagaaca actacaatat aaaaaaacta tacaaatgtc aagttcttga aaacaagaat
      241 ctttttattg tcagtactga tcagtcctgc tcctcggcca cgaagtgcac gcagttgccg
      301 gccgggtcgc gcagggcgaa ctcccgcccc cacggctgct cgccgatctc ggtcatggcc
      361 ggcccggagg cgtcccggaa gttcgtggac acgacctccg accactcggc gtacagctcg
      421 tccaggccgc gcacccacac ccaggccagg gtgttgtccg gcaccacctg gtcctggacc
      481 gcgctgatga acagggtcac gtcgtcccgg accacaccgg cgaagtcgtc ctccacgaag
      541 tcccgggaga acccgagccg gtcggtccag aactcgaccg ctccggcgac gtcgcgcgcg
      601 gtgagcaccg gaacggcgct ggtcaacttg gccatggttg tttatgttcg gatgtgatgt
      661 gagaactgta tcctagcaag attttaaaag gaagtatatg aaagaagaac ctcagtggca
      721 aatcctaacc ttttatattt ctctacaggg gcgcggcgtg gggacaattc aacgcgtctg
      781 tgaggggagc gtttccctgc tcgcagg
//
