LOCUS       sequence-477069-       14583 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression vector of Ribozyme split GFPuv with tEvopreq motif.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14583)
  AUTHORS   Liu Y, Rajput R, Islam MT, Valle ID, Yao T, Agrawal R, Boone BA, 
            Eckert CA, Abraham PE, Chen JG, Tuskan GA, Yang X
  TITLE     A split ribozyme system for in vivo plant RNA imaging and genetic 
            engineering.
  JOURNAL   Plant Biotechnol J. 2025 May;23(5):1640-1649. doi: 
            10.1111/pbi.14612. Epub 2025 Feb 7.
  PUBMED    39919021
REFERENCE   2  (bases 1 to 14583)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/pbi.14612";
            journalName: "Plant Biotechnol J"; date: "2025-05"; volume: "23"; 
            issue: "5"; pages: "1640-1649"
COMMENT     Sequence Label: pXYB106
FEATURES             Location/Qualifiers
     source          1..14583
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(16..361)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     723..745
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     737..754
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     738..754
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        1269..1614
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     terminator      2850..3098
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     primer_bind     complement(3788..3804)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3788..3804)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3801..3823)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3812..3828
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3836..3842,3843..3860,3861..3866))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3881..3902
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        4093..4769
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     CDS             4837..5634
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4894..4913)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5504..5523
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5674..5848
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    5926..5950
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6375..7169
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      7256..7844
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7745..7764
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7998..8015
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8030..8170
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8096..8115)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8256..8278
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      8514..8708
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8774..9847)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10276..10905)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    12206..12230
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(12252..12504)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(12536..12553)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(join(12560..14377,14568..14582))
                     /codon_start=1
                     /product="beta-glucuronidase"
                     /label=GUSPlus(TM)
                     /note="codon-optimized Staphylococcus gusA gene with a 
                     catalase intron to ensure expression in plants but not 
                     bacteria"
                     /translation="MVDLRNRRTSLYPINTETRGVFDLNGVWNFKLDYGKGLEEKWYES
                     KLTDTISMAVPSSYNDIGVTKEIRNHIGYVWYEREFTVPAYLKDQRIVLRFGSATHKAI
                     VYVNGELVVEHKGGFLPFEAEINNSLRDGMNRVTVAVDNILDDSTLPVGLYSERHEEGL
                     GKVIRNKPNFDFFNYAGLHRPVKIYTTPFTYVEDISVVTDFNGPTGTVTYTVDFQGKAE
                     TVKVSVVDEEGKVVASTEGLSGNVEIPNVILWEPLNTYLYQIKVELVNDGLTIDVYEEP
                     FGVRTVEVNDGKFLINNKPFYFKGFGKHEDTPINGRGFNEASNVMDFNILKWIGANSFR
                     TAHYPYSEELMRLADREGLVVIDETPAVGVHLNFMATTGLGEGSERVSTWEKIRTFEHH
                     QDVLRELVSRDKNHPSVVMWSIANEAATEEEGAYEYFKPLVELTKELDPQKRPVTIVLF
                     VMATPETDKVAELIDVIALNRYNGWYFDGGDLEAAKVHLRQEFHAWNKRCPGKPIMITE
                     YGADTVAGFHDIDPVMFTEEYQVEYYQANHVVFDEFENFVGEQAWNFADFATSQGVMRV
                     QGNKKGVFTRDRKPKLAAHVFRERWTNIPDFGYKN"
     intron          14378..14567
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
ORIGIN
        1 gtcaagagtc ccccgtgttc tctccaaatg aaatgaactt ccttatatag aggaagggtc
       61 ttgcgaagga tagtgggatt gtgcgtcatc ccttacgtca gtggagatat cacatcaatc
      121 cacttgcttt gaagacgtgg ttggaacgtc ttctttttcc acgatgctcc tcgtgggtgg
      181 gggtccatct ttgggaccac tgtcggcaga ggcatcttca acgatggcct ttcctttatc
      241 gcaatgatgg catttgtagg agccaccttc cttttccact atcttcacaa taaagtgaca
      301 gatagctggg caatggaatc cgaggaggtt tccggatatt accctttgtt gaaaagtctc
      361 aattgccctt tggtcttctg agactgtatc tttgatattt ttggagtaga caagtgtgtc
      421 gtgctccacc atgttgacga agattttctt cttgtcattg agtcgtaaga gactctgtat
      481 gaactgttcg ccagtcttta cggcgagttc tgttaggtcc tctatttgaa tctttgactc
      541 catgaagcta aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca
      601 ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt
      661 acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
      721 ttcccagtca cgacgttgta aaacgacggc cagtgccaag cttgcatgcc tgcaggtccc
      781 cagattagcc ttttcaattt cagaaagaat gctaacccac agatggttag agaggcttac
      841 gcagcaggtc tcatcaagac gatctacccg agcaataatc tccaggaaat caaatacctt
      901 cccaagaagg ttaaagatgc agtcaaaaga ttcaggacta actgcatcaa gaacacagag
      961 aaagatatat ttctcaagat cagaagtact attccagtat ggacgattca aggcttgctt
     1021 cacaaaccaa ggcaagtaat agagattgga gtctctaaaa aggtagttcc cactgaatca
     1081 aaggccatgg agtcaaagat tcaaatagag gacctaacag aactcgccgt aaagactggc
     1141 gaacagttca tacagagtct cttacgactc aatgacaaga agaaaatctt cgtcaacatg
     1201 gtggagcacg acacacttgt ctactccaaa aatatcaaag atacagtctc agaagaccaa
     1261 agggcaattg agacttttca acaaagggta atatccggaa acctcctcgg attccattgc
     1321 ccagctatct gtcactttat tgtgaagata gtggaaaagg aaggtggctc ctacaaatgc
     1381 catcattgcg ataaaggaaa ggccatcgtt gaagatgcct ctgccgacag tggtcccaaa
     1441 gatggacccc cacccacgag gagcatcgtg gaaaaagaag acgttccaac cacgtcttca
     1501 aagcaagtgg attgatgtga tatctccact gacgtaaggg atgacgcaca atcccactat
     1561 ccttcgcaag acccttcctc tatataagga agttcatttc atttggagag aacacggggg
     1621 actcaaacat tactcattca caaaaccatc ttaaagcaac tacacaagtc ttgaaatttt
     1681 ctcatatttt ctatttacta tataaacttt taatcaaatc aagattaaag ttaattaaat
     1741 gacaaccttc aaaatcgagt cccggatcca cggcaacctc aacggggaga agttcgagtt
     1801 ggttggaggt ggagtaggtg aggagggtcg cctcgagatt gagatgaaga ctaaagataa
     1861 accactggca ttctctccct tcctgctgac cacttgcatg ggttacgggt tctaccactt
     1921 cgccagcttc ccaaagggga ttaagaacat ctataaatag caatatttac ctttgggtca
     1981 aaaagttatc aggcatgcac ctggtagcta gtctttaaac caatagattg catcggttta
     2041 aaaggcaaga ccgtcaaatt gcgggaaagg ggtcaacagc cgttcagtac caagtctcag
     2101 gggaaacttt gagatggcct tgcaaagggt atggtaataa gctgacggac atggtcctaa
     2161 ccacgcagcc aagtcctaag tcaacagatc ttctgttgat atggatgcag ttcacagact
     2221 aaatgtcggt cggggaagat gtattcttct cataagatat agtcggacct ctccttaatg
     2281 ggagctagcg gatgaagtga tgcaacactg gagccgctgg gaactaattt gtatgcgaaa
     2341 gtatattgat tagttttgga gtactcgctt catgctgcaa cgaacggagg ttacaccaac
     2401 accaggaagg agatctatga agacggcggc atcttggagg tcaacttccg ttacacttac
     2461 gagttcaaca agatcatcgg tgacgtcgag tgcattggac atggattccc aagtcagagt
     2521 ccgatcttca aggacacgat cgtgaagagt tgtcccacgg tggacctgat gttgccaatg
     2581 tccgggaaca tcatcgccag ctcctacgct tacgccttcc aactgaagga cggctctttc
     2641 tacacggcag aagtcaagaa caacatagac ttcaagaatc caatccacga gtccttctcg
     2701 aagtcagggc ccatgttcac ccacagacgt gtcgaggaga ctctcaccaa ggagaacctt
     2761 gccatagtgg agtaccagca ggttttcaac agcgccccaa gagacatgta gcgcggttct
     2821 atctagttac gcgttaaacc aactagaaat atgaagatga agatgaaata tttggtgtgt
     2881 caaataaaaa gcttgtgtgc ttaagtttgt gtttttttct tggcttgttg tgttatgaat
     2941 ttgtggcttt ttctaatatt aaatgaatgt aagatctcat tataatgaat aaacaaatgt
     3001 ttctataatc cattgtgaat gttttgttgg atctcttctg cagcatataa ctactgtatg
     3061 tgctatggta tggactatgg aatatgatta aagataagat gggctcatag agtaaaacga
     3121 ggcgagggac ctataaacct cccttcatca tgctatttca tgatctattt tataaaataa
     3181 agatgtagaa aaaagtaagc gtaataaccg caaaacaaat gatttaaaac atggcacata
     3241 atgaggagat taagttcggt ttacgtttat tttagtacta attgtaacgt gagactacgt
     3301 atcgggaatc gcctaattaa agcattaatg cgaacctgat tagattcacc gaccctccta
     3361 tcgtgtcgac ctttctgttt cttagaattt tttggtagtc tatgtactaa taatgtcagc
     3421 ttcgtattta tttcataagc aatttgcatt tgcaatttgt tttttacttt tatttttatt
     3481 gtattgtgga atgtggactc gtaccaacat gaagttatat accaccaaaa aaattacagt
     3541 tagtcaaaag attcacgagt gagagctact tatgattgtc ttttacgtat atgtctaatt
     3601 gtctatttgc tcaataatct ttgtactttc ttttgtcgtt gataaaatca caaagttcca
     3661 aaagtaatcg aatgatttgc ttttaagaaa agaagagctc aataattcaa catatatctg
     3721 tacacaatta ccgcctttga gtgagctcta gatgccacct gacgtctaag aagaattcgt
     3781 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     3841 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     3901 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     3961 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tggctagagc agcttgccaa
     4021 catggtggag cacgacactc tcgtctactc caagaatatc aaagatacag tctcagaaga
     4081 ccaaagggct attgagactt ttcaacaaag ggtaatatcg ggaaacctcc tcggattcca
     4141 ttgcccagct atctgtcact tcatcaaaag gacagtagaa aaggaaggtg gcacctacaa
     4201 atgccatcat tgcgataaag gaaaggctat cgttcaagat gcctctgccg acagtggtcc
     4261 caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagacgttc caaccacgtc
     4321 ttcaaagcaa gtggattgat gtgaacatgg tggagcacga cactctcgtc tactccaaga
     4381 atatcaaaga tacagtctca gaagaccaaa gggctattga gacttttcaa caaagggtaa
     4441 tatcgggaaa cctcctcgga ttccattgcc cagctatctg tcacttcatc aaaaggacag
     4501 tagaaaagga aggtggcacc tacaaatgcc atcattgcga taaaggaaag gctatcgttc
     4561 aagatgcctc tgccgacagt ggtcccaaag atggaccccc acccacgagg agcatcgtgg
     4621 aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga ttgatgtgat atctccactg
     4681 acgtaaggga tgacgcacaa tcccactatc cttcgcaaga cccttcctct atataaggaa
     4741 gttcatttca tttggagagg acacgctgaa atcaccagtc tctctctaca aatctatctc
     4801 tctcgagctt tcgcagatcc cggggggcaa tgagatatgg ggattgaaca agatggattg
     4861 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
     4921 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
     4981 tttgtcaaga ccgacctgtc cggtgccctg aatgaactcc aggacgaggc agcgcggcta
     5041 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
     5101 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
     5161 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
     5221 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
     5281 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
     5341 gccgaactgt tcgccaggct caaggcgcgc atgcccgacg gcgaggatct cgtcgtgaca
     5401 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
     5461 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
     5521 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
     5581 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgaagtaga
     5641 tgccgaccgg atctgtcgat cgacaagctc gagtttctcc ataataatgt gtgagtagtt
     5701 cccagataag ggaattaggg ttcctatagg gtttcgctca tgtgttgagc atataagaaa
     5761 cccttagtat gtatttgtat ttgtaaaata cttctatcaa taaaatttct aattcctaaa
     5821 accaaaatcc agtactaaaa tccagatccc ccgaattaat tcggcgttaa ttcagtacat
     5881 taaaaacgtc cgcaatgtgt tattaagttg tctaagcgtc aatttgttta caccacaata
     5941 tatcctgcca ccagccagcc aacagctccc cgaccggcag ctcggcacaa aatcaccact
     6001 cgatacaggc agcccatcag tccgggacgg cgtcagcggg agagccgttg taaggcggca
     6061 gactttgctc atgttaccga tgctattcgg aagaacggca actaagctgc cgggtttgaa
     6121 acacggatga tctcgcggag ggtagcatgt tgattgtaac gatgacagag cgttgctgcc
     6181 tgtgatcacc gcggtttcaa aatcggctcc gtcgatacta tgttatacgc caactttgaa
     6241 aacaactttg aaaaagctgt tttctggtat ttaaggtttt agaatgcaag gaacagtgaa
     6301 ttggagttcg tcttgttata attagcttct tggggtatct ttaaatactg tagaaaagag
     6361 gaaggaaata ataaatggct aaaatgagaa tatcaccgga attgaaaaaa ctgatcgaaa
     6421 aataccgctg cgtaaaagat acggaaggaa tgtctcctgc taaggtatat aagctggtgg
     6481 gagaaaatga aaacctatat ttaaaaatga cggacagccg gtataaaggg accacctatg
     6541 atgtggaacg ggaaaaggac atgatgctat ggctggaagg aaagctgcct gttccaaagg
     6601 tcctgcactt tgaacggcat gatggctgga gcaatctgct catgagtgag gccgatggcg
     6661 tcctttgctc ggaagagtat gaagatgaac aaagccctga aaagattatc gagctgtatg
     6721 cggagtgcat caggctcttt cactccatcg acatatcgga ttgtccctat acgaatagct
     6781 tagacagccg cttagccgaa ttggattact tactgaataa cgatctggcc gatgtggatt
     6841 gcgaaaactg ggaagaagac actccattta aagatccgcg cgagctgtat gattttttaa
     6901 agacggaaaa gcccgaagag gaacttgtct tttcccacgg cgacctggga gacagcaaca
     6961 tctttgtgaa agatggcaaa gtaagtggct ttattgatct tgggagaagc ggcagggcgg
     7021 acaagtggta tgacattgcc ttctgcgtcc ggtcgatcag ggaggatatc ggggaagaac
     7081 agtatgtcga gctatttttt gacttactgg ggatcaagcc tgattgggag aaaataaaat
     7141 attatatttt actggatgaa ttgttttagt acctagaatg catgaccaaa atcccttaac
     7201 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     7261 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     7321 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     7381 gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga
     7441 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     7501 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     7561 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     7621 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     7681 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     7741 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     7801 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     7861 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     7921 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     7981 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt
     8041 attttctcct tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa
     8101 tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt
     8161 catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     8221 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     8281 ttcaccgtca tcaccgaaac gcgcgaggca gggtgccttg atgtgggcgc cggcggtcga
     8341 gtggcgacgg cgcggcttgt ccgcgccctg gtagattgcc tggccgtagg ccagccattt
     8401 ttgagcggcc agcggccgcg ataggccgac gcgaagcggc ggggcgtagg gagcgcagcg
     8461 accgaagggt aggcgctttt tgcagctctt cggctgtgcg ctggccagac agttatgcac
     8521 aggccaggcg ggttttaaga gttttaataa gttttaaaga gttttaggcg gaaaaatcgc
     8581 cttttttctc ttttatatca gtcacttaca tgtgtgaccg gttcccaatg tacggctttg
     8641 ggttcccaat gtacgggttc cggttcccaa tgtacggctt tgggttccca atgtacgtgc
     8701 tatccacagg aaagagacct tttcgacctt tttcccctgc tagggcaatt tgccctagca
     8761 tctgctccgt acattaggaa ccggcggatg cttcgccctc gatcaggttg cggtagcgca
     8821 tgactaggat cgggccagcc tgccccgcct cctccttcaa atcgtactcc ggcaggtcat
     8881 ttgacccgat cagcttgcgc acggtgaaac agaacttctt gaactctccg gcgctgccac
     8941 tgcgttcgta gatcgtcttg aacaaccatc tggcttctgc cttgcctgcg gcgcggcgtg
     9001 ccaggcggta gagaaaacgg ccgatgccgg gatcgatcaa aaagtaatcg gggtgaaccg
     9061 tcagcacgtc cgggttcttg ccttctgtga tctcgcggta catccaatca gctagctcga
     9121 tctcgatgta ctccggccgc ccggtttcgc tctttacgat cttgtagcgg ctaatcaagg
     9181 cttcaccctc ggataccgtc accaggcggc cgttcttggc cttcttcgta cgctgcatgg
     9241 caacgtgcgt ggtgtttaac cgaatgcagg tttctaccag gtcgtctttc tgctttccgc
     9301 catcggctcg ccggcagaac ttgagtacgt ccgcaacgtg tggacggaac acgcggccgg
     9361 gcttgtctcc cttcccttcc cggtatcggt tcatggattc ggttagatgg gaaaccgcca
     9421 tcagtaccag gtcgtaatcc cacacactgg ccatgccggc cggccctgcg gaaacctcta
     9481 cgtgcccgtc tggaagctcg tagcggatca cctcgccagc tcgtcggtca cgcttcgaca
     9541 gacggaaaac ggccacgtcc atgatgctgc gactatcgcg ggtgcccacg tcatagagca
     9601 tcggaacgaa aaaatctggt tgctcgtcgc ccttgggcgg cttcctaatc gacggcgcac
     9661 cggctgccgg cggttgccgg gattctttgc ggattcgatc agcggccgct tgccacgatt
     9721 caccggggcg tgcttctgcc tcgatgcgtt gccgctgggc ggcctgcgcg gccttcaact
     9781 tctccaccag gtcatcaccc agcgccgcgc cgatttgtac cgggccggat ggtttgcgac
     9841 cgctcacgcc gattcctcgg gcttgggggt tccagtgcca ttgcagggcc ggcaggcaac
     9901 ccagccgctt acgcctggcc aaccgcccgt tcctccacac atggggcatt ccacggcgtc
     9961 ggtgcctggt tgttcttgat tttccatgcc gcctccttta gccgctaaaa ttcatctact
    10021 catttattca tttgctcatt tactctggta gctgcgcgat gtattcagat agcagctcgg
    10081 taatggtctt gccttggcgt accgcgtaca tcttcagctt ggtgtgatcc tccgccggca
    10141 actgaaagtt gacccgcttc atggctggcg tgtctgccag gctggccaac gttgcagcct
    10201 tgctgctgcg tgcgctcgga cggccggcac ttagcgtgtt tgtgcttttg ctcattttct
    10261 ctttacctca ttaactcaaa tgagttttga tttaatttca gcggccagcg cctggacctc
    10321 gcgggcagcg tcgccctcgg gttctgattc aagaacggtt gtgccggcgg cggcagtgcc
    10381 tgggtagctc acgcgctgcg tgatacggga ctcaagaatg ggcagctcgt acccggccag
    10441 cgcctcggca acctcaccgc cgatgcgcgt gcctttgatc gcccgcgaca cgacaaaggc
    10501 cgcttgtagc cttccatccg tgacctcaat gcgctgctta accagctcca ccaggtcggc
    10561 ggtggcccat atgtcgtaag ggcttggctg caccggaatc agcacgaagt cggctgcctt
    10621 gatcgcggac acagccaagt ccgccgcctg gggcgctccg tcgatcacta cgaagtcgcg
    10681 ccggccgatg gccttcacgt cgcggtcaat cgtcgggcgg tcgatgccga caacggttag
    10741 cggttgatct tcccgcacgg ccgcccaatc gcgggcactg ccctggggat cggaatcgac
    10801 taacagaaca tcggccccgg cgagttgcag ggcgcgggct agatgggttg cgatggtcgt
    10861 cttgcctgac ccgcctttct ggttaagtac agcgataacc ttcatgcgtt ccccttgcgt
    10921 atttgtttat ttactcatcg catcatatac gcagcgaccg catgacgcaa gctgttttac
    10981 tcaaatacac atcacctttt tagacggcgg cgctcggttt cttcagcggc caagctggcc
    11041 ggccaggccg ccagcttggc atcagacaaa ccggccagga tttcatgcag ccgcacggtt
    11101 gagacgtgcg cgggcggctc gaacacgtac ccggccgcga tcatctccgc ctcgatctct
    11161 tcggtaatga aaaacggttc gtcctggccg tcctggtgcg gtttcatgct tgttcctctt
    11221 ggcgttcatt ctcggcggcc gccagggcgt cggcctcggt caatgcgtcc tcacggaagg
    11281 caccgcgccg cctggcctcg gtgggcgtca cttcctcgct gcgctcaagt gcgcggtaca
    11341 gggtcgagcg atgcacgcca agcagtgcag ccgcctcttt cacggtgcgg ccttcctggt
    11401 cgatcagctc gcgggcgtgc gcgatctgtg ccggggtgag ggtagggcgg gggccaaact
    11461 tcacgcctcg ggccttggcg gcctcgcgcc cgctccgggt gcggtcgatg attagggaac
    11521 gctcgaactc ggcaatgccg gcgaacacgg tcaacaccat gcggccggcc ggcgtggtgg
    11581 tgtcggccca cggctctgcc aggctacgca ggcccgcgcc ggcctcctgg atgcgctcgg
    11641 caatgtccag taggtcgcgg gtgctgcggg ccaggcggtc tagcctggtc actgtcacaa
    11701 cgtcgccagg gcgtaggtgg tcaagcatcc tggccagctc cgggcggtcg cgcctggtgc
    11761 cggtgatctt ctcggaaaac agcttggtgc agccggccgc gtgcagttcg gcccgttggt
    11821 tggtcaagtc ctggtcgtcg gtgctgacgc gggcatagcc cagcaggcca gcggcggcgc
    11881 tcttgttcat ggcgtaatgt ctccggttct agtcgcaagt attctacttt atgcgactaa
    11941 aacacgcgac aagaaaacgc caggaaaagg gcagggcggc agcctgtcgc gtaacttagg
    12001 acttgtgcga catgtcgttt tcagaagacg gctgcactga acgtcagaag ccgactgcac
    12061 tatagcagcg gaggggttgg atcaaagtac tttgatcccg aggggaaccc tgtggttggc
    12121 atgcacatac aaatggacga acggataaac cttttcacgc ccttttaaat atccgattat
    12181 tctaataaac gctcttttct cttaggttta cccgccaata tatcctgtca aacactgata
    12241 gtttaattcc cgatctagta acatagatga caccgcgcgc gataatttat cctagtttgc
    12301 gcgctatatt ttgttttcta tcgcgtatta aatgtataat tgcgggactc taatcataaa
    12361 aacccatctc ataaataacg tcatgcatta catgttaatt attacatgct taacgtaatt
    12421 caacagaaat tatatgataa tcatcgcaag accggcaaca ggattcaatc ttaagaaact
    12481 ttattgccaa atgtttgaac gatcggggaa attcgagctg gtcaccaatt cacacgtgat
    12541 ggtgatggtg atggctagcg ttcttgtagc cgaaatctgg aatgttggtc cagcgctcgc
    12601 gaaagacgtg cgcggcgagc ttcggcttgc ggtcacgagt gaacacgccc ttcttgtttc
    12661 cttggacgcg catcacgccc tgagaggtcg cgaagtccgc gaagttccac gcttgctcac
    12721 ccacgaagtt ctcaaactca tcgaacacga cgtggttcgc ctggtagtac tcgacttgat
    12781 attcctcggt gaacatcact ggatcaatgt cgtgaaagcc cgcaacggtg tctgcgccgt
    12841 actcagtgat catgatcggc tttcctgggc aacgcttgtt ccacgcgtga aattcctggc
    12901 ggagatggac tttggccgct tcgagatcac cgccatcgaa gtaccatccg ttatagcgat
    12961 tgagcgcgat gacgtcaatc agttcggcga ctttgtccgt ctccggggta gccatcacaa
    13021 acagcacgat cgtgaccgga cgcttctgtg ggtcgagttc cttggtcagc tccaccaacg
    13081 gcttgaagta ctcgtacgcg ccctcttcct cagtcgccgc ctcgttggcg atgctccaca
    13141 tcacgacgct tggatggttc ttgtcacgag acaccagttc acggagaacg tcttgatggt
    13201 gctcaaacgt ccgaatcttc tcccaggtac tgacgcgctc gctgccttcg ccgagtcccg
    13261 tggtggccat gaagttgagg tgcacgccaa ctgccggagt ctcgtcgatc acgaccagac
    13321 cctcgcgatc cgcaagacgc atcaactctt cagagtacgg atagtgtgcg gtccggaagc
    13381 tgttggcgcc gatccatttg aggatattga aatccatcac attgctcgct tcgttaaagc
    13441 cacggccgtt gataggagtg tcctcatgtt tgccaaagcc cttgaagtag aacggtttgt
    13501 tgttgatgag gaacttgccg tcgttgactt ccacggtccg cacgccgaac ggctcttcat
    13561 agacatcgat ggtcagtccg tcgttcacca gttccacttt gatctggtag agatacgtgt
    13621 tcagtggttc ccagaggatg acattcggaa tctccacgtt accgctcagg ccctcggtgc
    13681 ttgcgaccac tttgccttcc tcatccacga ccgacacttt cacggtctcg gctttgcctt
    13741 gaaagtccac cgtataggtc acagtcccgg ttgggccatt gaagtcggtc acaaccgaga
    13801 tgtcctcgac gtacgtaaac ggggtcgtgt agattttcac cggacggtgc aggcctgcat
    13861 agttgaagaa gtcgaagttc ggcttgttac gaatgacttt tccgaggccc tcttcgtggc
    13921 gctcgctgta cagccccacc gggagggtgc tatcgtcgag gatgttgtcc acggcgacgg
    13981 tgacgcgatt catgccatca cgcagcgagt tgttgatttc cgcttcgaat ggcaggaatc
    14041 cgcccttgtg ctccacgacc agctcaccat tgacatagac aattgctttg tgagttgcag
    14101 agccgaagcg gagcacgata cgctgatcct tcagataggc cggcaccgtg aactcacgtt
    14161 cgtaccagac atatccgata tggttgcgga tttccttggt cacgccaatg tcattgtaac
    14221 tgcttgggac ggccatacta atagtgtcgg tcagcttgct ttcgtaccac ttctcttcca
    14281 gtcctttccc gtagtccagc ttgaagttcc agacgccatt gaggtcgaag acgccacggg
    14341 tctcggtgtt gatcgggtac agactagttc gtcggttctg taactatcat catcatcata
    14401 gacacacgaa ataaagtaat cagattatca gttaaagcta tgtaatattt acaccataac
    14461 caatcaatta aaaaatagat cagtttaaag aaagatcaaa gctcaaaaaa ataaaaagag
    14521 aaaagggtcc taaccaagaa aatgaaggag aaaaactaga aatttaccct cagatctacc
    14581 atg
//
