LOCUS       sequence-47713-e         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Young CL, Raden DL, Caplan JL, Czymmek KJ, Robinson AS
  TITLE     Cassette series designed for live-cell imaging of proteins and 
            high-resolution techniques in yeast.
  JOURNAL   Yeast. 2012 Mar;29(3-4):119-36. doi: 10.1002/yea.2895. Epub 2012 Apr
            4.
  PUBMED    22473760
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Yeast."; 
            date: "2012-03"; volume: "29(3-4):119-36. doi"; pages: " 
            10.1002/yea.2895. Epub 2012 Apr 4"
COMMENT     Sequence Label: pCY 3120-01
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             43..756
                     /codon_start=1
                     /product="yeast-enhanced green fluorescent protein"
                     /label=yeGFP
                     /note="codons optimized for translation in S. cerevisiae 
                     and C. albicans"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLGYGLMCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGIDELYK"
     CDS             775..804
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             811..840
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 gatccccggg ttattaacgg tgacggtgct ggtttaatta acatgtctaa aggtgaagaa
       61 ttattcactg gtgttgtccc aattttggtt gaattagatg gtgatgttaa tggtcacaaa
      121 ttttctgtct ccggtgaagg tgaaggtgat gctacttacg gtaaattgac cttaaaattt
      181 atttgtacta ctggtaaatt gccagttcca tggccaacct tagtcactac tttaggttat
      241 ggtttgatgt gttttgctag atacccagat catatgaaac aacatgactt tttcaagtct
      301 gccatgccag aaggttatgt tcaagaaaga actatttttt tcaaagatga cggtaactac
      361 aagaccagag ctgaagtcaa gtttgaaggt gataccttag ttaatagaat cgaattaaaa
      421 ggtattgatt ttaaagaaga tggtaacatt ttaggtcaca aattggaata caactataac
      481 tctcacaatg tttacatcat ggctgacaaa caaaagaatg gtatcaaagt taacttcaaa
      541 attagacaca acattgaaga tggttctgtt caattagctg accattatca acaaaatact
      601 ccaattggtg atggtccagt cttgttacca gacaaccatt acttatccta tcaatctgcc
      661 ttatccaaag atccaaacga aaagagagac cacatggtct tgttagaatt tgttactgct
      721 gctggtatta cccatggtat tgatgaattg tacaaacccg ggttaattaa cggtgaacaa
      781 aagctaatct ccgaggaaga cttgaacggt gaacaaaaat taatctcaga agaagacttg
      841 aacgga
//
