LOCUS       sequence-47729-e         765 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Kato HE, Zhang F, Yizhar O, Ramakrishnan C, Nishizawa T, Hirata K, 
            Ito J, Aita Y, Tsukazaki T, Hayashi S, Hegemann P, Maturana AD, 
            Ishitani R, Deisseroth K, Nureki O
  TITLE     Crystal structure of the channelrhodopsin light-gated cation 
            channel.
  JOURNAL   Nature. 2012 Jan 22. doi: 10.1038/nature10870.
  PUBMED    22266941
REFERENCE   2  (bases 1 to 765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2012 Jan 22. doi: 10.1038/nature10870."
COMMENT     Sequence Label: pLenti-CaMKIIa-C1C2-EYFP
FEATURES             Location/Qualifiers
     source          1..765
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(49..762,763..765))
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(94..115)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     443..462
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     702..723
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 aattttgtaa tccagaggtt gattatcgat aagcttgata tcgaattctt acttgtacag
       61 ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac tccagcagga ccatgtgatc
      121 gcgcttctcg ttggggtctt tgctcagggc ggactggtag ctcaggtagt ggttgtcggg
      181 cagcagcacg gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg cgagctgcac
      241 gctgccgtcc tcgatgttgt ggcggatctt gaagttcacc ttgatgccgt tcttctgctt
      301 gtcggccatg atatagacgt tgtggctgtt gtagttgtac tccagcttgt gccccaggat
      361 gttgccgtcc tccttgaagt cgatgccctt cagctcgatg cggttcacca gggtgtcgcc
      421 ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg tccttgaaga agatggtgcg
      481 ctcctggacg tagccttcgg gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc
      541 ggggtagcgg gcgaagcact gcaggccgta gccgaaggtg gtcacgaggg tgggccaggg
      601 cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt aggtggcatc
      661 gccctcgccc tcgccggaca cgctgaactt gtggccgttt acgtcgccgt ccagctcgac
      721 caggatgggc accaccccgg tgaacagctc ctcgcccttg ctcac
//
