LOCUS       sequence-47823-e         808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses tdTomato in cells lacking Cre (Cre-Off, flanked by 
            alternative lox FAS sites).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Saunders A, Johnson CA, Sabatini BL
  TITLE     Novel recombinant adeno-associated viruses for Cre activated and 
            inactivated transgene expression in neurons.
  JOURNAL   Front Neural Circuits. 2012 Jul 27;6:47. doi: 
            10.3389/fncir.2012.00047. eCollection 2012.
  PUBMED    22866029
REFERENCE   2  (bases 1 to 808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Neural Circuits."; date: "2012-07-27"; volume: "6:47. doi"; pages: "
            10.3389/fncir.2012.00047. eCollection 2012"
COMMENT     Sequence Label: pAAV-Ef1a-FAS-TdTomato-WPRE-pA
FEATURES             Location/Qualifiers
     source          1..808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      79..88
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(218..236)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     486..505
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     680..703
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 tcgtgaggta ccggatcctc tagagtcgac tccggaacaa cttcgtatat acctttctat
       61 acgaagttgt ggcgcgccgc caccatggtg agcaagggcg aggaggtcat caaagagttc
      121 atgcgcttca aggtgcgcat ggagggctcc atgaacggcc acgagttcga gatcgagggc
      181 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc
      241 ggccccctgc ccttcgcctg ggacatcctg tccccccagt tcatgtacgg ctccaaggcg
      301 tacgtgaagc accccgccga catccccgat tacaagaagc tgtccttccc cgagggcttc
      361 aagtgggagc gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac ccaggactcc
      421 tccctgcagg acggcacgct gatctacaag gtgaagatgc gcggcaccaa cttccccccc
      481 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctccaccga gcgcctgtac
      541 ccccgcgacg gcgtgctgaa gggcgagatc caccaggccc tgaagctgaa ggacggcggc
      601 cactacctgg tggagttcaa gaccatctac atggccaaga agcccgtgca actgcccggc
      661 tactactacg tggacaccaa gctggacatc acctcccaca acgaggacta caccatcgtg
      721 gaacagtacg agcgctccga gggccgccac cacctgttcc tggggcatgg caccggcagc
      781 accggcagcg gcagctccgg caccgcct
//
