LOCUS       sequence-478503-       11933 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express CBP-NanoLuc Fusion Protein in Mammalian Cells under a CMV 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11933)
  AUTHORS   Promega
  TITLE     Promega Plasmid Collection
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: CBP-NanoLuc Fusion Vector
FEATURES             Location/Qualifiers
     source          1..11933
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        138..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     672..692
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     972..991
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     primer_bind     1033..1052
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1033..1051
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      5931..5940
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8412..8924
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="VFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRI
                     VLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVT
                     PNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWR
                     LCERILA"
     primer_bind     complement(9012..9030)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     polyA_signal    9067..9188
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9078..9097)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     9132..9151
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        9433..9790
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        9433..9624
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(9456..9476)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      9641..9776
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        9805..9852
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9884..10678
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9938..9957)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10548..10567
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    10742..10790
                     /note="synthetic polyadenylation signal"
     rep_origin      10947..11492
                     /direction=RIGHT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     11393..11412
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11609..11626
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tcaatattgg ccattagcca tattattcat tggttatata gcataaatca atattggcta
       61 ttggccattg catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc
      121 aatatgaccg ccatgttggc attgattatt gactagttat taatagtaat caattacggg
      181 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
      241 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
      301 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
      361 ccacttggca gtacatcaag tgtatcatat gccaagtccg ccccctattg acgtcaatga
      421 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg
      481 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac
      541 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
      601 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc
      661 cgccccgttg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
      721 tggtttagtg aaccgtcaga tcactagaag ctttattgcg gtagtttatc acagttaaat
      781 tgctaacgca gtcagtgctt ctgacacaac agtctcgaac ttaagctgca gaagttggtc
      841 gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag accaatagaa
      901 actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta ttggtcttac
      961 tgacatccac tttgcctttc tctccacagg tgtccactcc cagttcaatt acagctctta
     1021 aggctagagt attaatacga ctcactatag ggctagcgat cgccatggct gagaacttgc
     1081 tggacggacc gcccaacccc aaaagagcca aactcagctc gcccggtttc tcggcgaatg
     1141 acagcacaga ttttggatca ttgtttgact tggaaaatga tcttcctgat gagctgatac
     1201 ccaatggagg agaattaggc cttttaaaca gtgggaacct tgttccagat gctgcttcca
     1261 aacataaaca actgtcggag cttctacgag gaggcagcgg ctctagtatc aacccaggaa
     1321 taggaaatgt gagcgccagc agccccgtgc agcagggcct gggtggccag gctcaagggc
     1381 agccgaacag tgctaacatg gccagcctca gtgccatggg caagagccct ctgagccagg
     1441 gagattcttc agcccccagc ctgcctaaac aggcagccag cacctctggg cccacccccg
     1501 ctgcctccca agcactgaat ccgcaagcac aaaagcaagt ggggctggcg actagcagcc
     1561 ctgccacgtc acagactgga cctggtatct gcatgaatgc taactttaac cagacccacc
     1621 caggcctcct caatagtaac tctggccata gcttaattaa tcaggcttca caagggcagg
     1681 cgcaagtcat gaatggatct cttggggctg ctggcagagg aaggggagct ggaatgccgt
     1741 accctactcc agccatgcag ggcgcctcga gcagcgtgct ggctgagacc ctaacgcagg
     1801 tttccccgca aatgactggt cacgcgggac tgaacaccgc acaggcagga ggcatggcca
     1861 agatgggaat aactgggaac acaagtccat ttggacagcc ctttagtcaa gctggagggc
     1921 agccaatggg agccactgga gtgaaccccc agttagccag caaacagagc atggtcaaca
     1981 gtttgcccac cttccctaca gatatcaaga atacttcagt caccaacgtg ccaaatatgt
     2041 ctcagatgca aacatcagtg ggaattgtac ccacacaagc aattgcaaca ggccccactg
     2101 cagatcctga aaaacgcaaa ctgatacagc agcagctggt tctactgctt catgctcata
     2161 agtgtcagag acgagagcaa gcaaacggag aggttcgggc ctgctcgctc ccgcattgtc
     2221 gaaccatgaa aaacgttttg aatcacatga cgcattgtca ggctgggaaa gcctgccaag
     2281 ttgcccattg tgcatcttca cgacaaatca tctctcattg gaagaactgc acacgacatg
     2341 actgtcctgt ttgcctccct ttgaaaaatg ccagtgacaa gcgaaaccaa caaaccatcc
     2401 tggggtctcc agctagtgga attcaaaaca caattggttc tgttggcaca gggcaacaga
     2461 atgccacttc tttaagtaac ccaaatccca tagaccccag ctccatgcag cgagcctatg
     2521 ctgctctcgg actcccctac atgaaccagc cccagacgca gctgcagcct caggttcctg
     2581 gccagcaacc agcacagcct caaacccacc agcagatgag gactctcaac cccctgggaa
     2641 ataatccaat gaacattcca gcaggaggaa taacaacaga tcagcagccc ccaaacttga
     2701 tttcagaatc agctcttccg acttccctgg gggccacaaa cccactgatg aacgatggct
     2761 ccaactctgg taacattgga accctcagca ctataccaac agcagctcct ccttctagca
     2821 ccggtgtaag gaaaggctgg cacgaacatg tcactcagga cctgcggagc catctagtgc
     2881 ataaactcgt ccaagccatc ttcccaacac ctgatcccgc agctctaaag gatcgccgca
     2941 tggaaaacct ggtagcctat gctaagaaag tggaagggga catgtacgag tctgccaaca
     3001 gcagggatga atattatcac ttattagcag agaaaatcta caagatacaa aaagaactag
     3061 aagaaaaacg gaggtcgcgt ttacataaac aaggcatctt ggggaaccag ccagccttac
     3121 cagccccggg ggctcagccc cctgtgattc cacaggcaca acctgtgaga cctccaaatg
     3181 gacccctgtc cctgccagtg aatcgcatgc aagtttctca agggatgaat tcatttaacc
     3241 ccatgtcctt ggggaacgtc cagttgccac aagcacccat gggacctcgt gcagcctccc
     3301 caatgaacca ctctgtccag atgaacagca tgggctcagt gccagggatg gccatttctc
     3361 cttcccgaat gcctcagcct ccgaacatga tgggtgcaca caccaacaac atgatggccc
     3421 aggcgcccgc tcagagccag tttctgccac agaaccagtt cccgtcatcc agcggggcga
     3481 tgagtgtggg catggggcag ccgccagccc aaacaggcgt gtcacaggga caggtgcctg
     3541 gtgctgctct tcctaaccct ctcaacatgc tggggcctca ggccagccag ctaccttgcc
     3601 ctccagtgac acagtcacca ctgcacccaa caccgcctcc tgcttccacg gctgctggca
     3661 tgccatctct ccagcacacg acaccacctg ggatgactcc tccccagcca gcagctccca
     3721 ctcagccatc aactcctgtg tcgtcttccg ggcagactcc caccccgact cctggctcag
     3781 tgcccagtgc tacccaaacc cagagcaccc ctacagtcca ggcagcagcc caggcccagg
     3841 tgaccccgca gcctcaaacc ccagttcagc ccccgtctgt ggctacccct cagtcatcgc
     3901 agcaacagcc gacgcctgtg cacgcccagc ctcctggcac accgctttcc caggcagcag
     3961 ccagcattga taacagagtc cctaccccct cctcggtggc cagcgcagaa accaattccc
     4021 agcagccagg acctgacgta cctgtgctgg aaatgaagac ggagacccaa gcagaggaca
     4081 ctgagcccga tcctggtgaa tccaaagggg agcccaggtc tgagatgatg gaggaggatt
     4141 tgcaaggagc ttcccaagtt aaagaagaaa cagacatagc agagcagaaa tcagaaccaa
     4201 tggaagtgga tgaaaagaaa cctgaagtga aagtagaagt taaagaggaa gaagagagta
     4261 gcagtaacgg cacagcctct cagtcaacat ctccttcgca gccgcgcaaa aaaatcttta
     4321 aaccagagga gttacgccag gccctcatgc caaccctaga agcactgtat cgacaggacc
     4381 cagagtcatt acctttccgg cagcctgtag atccccagct cctcggaatt ccagactatt
     4441 ttgacatcgt aaagaatccc atggacctct ccaccatcaa gcggaagctg gacacagggc
     4501 aataccaaga gccctggcag tacgtggacg acgtctggct catgttcaac aatgcctggc
     4561 tctataatcg caagacatcc cgagtctata agttttgcag taagcttgca gaggtctttg
     4621 agcaggaaat tgaccctgtc atgcagtccc ttggatattg ctgtggacgc aagtatgagt
     4681 tttccccaca gactttgtgc tgctatggga agcagctgtg taccattcct cgcgatgctg
     4741 cctactacag ctatcagaat aggtatcatt tctgtgagaa gtgtttcaca gagatccagg
     4801 gcgagaatgt gaccctgggt gacgaccctt cacagcccca gacgacaatt tcaaaggatc
     4861 agtttgaaaa gaagaaaaat gataccttag accccgaacc tttcgttgat tgcaaggagt
     4921 gtggccggaa gatgcatcag atttgcgttc tgcactatga catcatttgg ccttcaggtt
     4981 ttgtgtgcga caactgcttg aagaaaactg gcagacctcg aaaagaaaac aaattcagtg
     5041 ctaagaggct gcagaccaca agactgggaa accacttgga agaccgagtg aacaaatttt
     5101 tgcggcgcca gaatcaccct gaagccgggg aggtttttgt ccgagtggtg gccagctcag
     5161 acaagacggt ggaggtcaag cccgggatga agtcacggtt tgtggattct ggggaaatgt
     5221 ctgaatcttt cccatatcga accaaagctc tgtttgcttt tgaggaaatt gacggcgtgg
     5281 atgtctgctt ttttggaatg cacgtccaag aatacggctc tgattgcccc cctccaaaca
     5341 cgaggcgtgt gtacatttct tatctggata gtattcattt cttccggcca cgttgcctcc
     5401 gcacagccgt ttaccatgag atccttattg gatatttaga gtatgtgaag aaattagggt
     5461 atgtgacagg gcacatctgg gcctgtcctc caagtgaagg agatgattac atcttccatt
     5521 gccacccacc tgatcaaaaa atacccaagc caaaacgact gcaggagtgg tacaaaaaga
     5581 tgctggacaa ggcgtttgca gagcggatca tccatgacta caaggatatt ttcaaacaag
     5641 caactgaaga caggctcacc agtgccaagg aactgcccta ttttgaaggt gatttctggc
     5701 ccaatgtgtt agaagagagc attaaggaac tagaacaaga agaagaggag aggaaaaagg
     5761 aagagagcac tgcagccagt gaaaccactg agggcagtca gggcgacagc aagaatgcca
     5821 agaagaagaa caacaagaaa accaacaaga acaaaagcag catcagccgc gccaacaaga
     5881 agaagcccag catgcccaac gtgtccaatg acctgtccca gaagctgtat gccaccatgg
     5941 agaagcacaa ggaggtcttc ttcgtgatcc acctgcacgc tgggcctgtc atcaacaccc
     6001 tgccccccat cgtcgacccc gaccccctgc tcagctgtga cctcatggat gggcgcgacg
     6061 ccttcctcac cctcgccaga gacaagcact gggagttctc ctccttgcgc cgctccaagt
     6121 ggtccacgct ctgcatgctg gtggagctgc acacccaggg ccaggaccgc tttgtctaca
     6181 cctgcaacga gtgcaagcac cacgtggaga cgcgctggca ctgcactgtg tgcgaggact
     6241 acgacctctg catcaactgc tataacacga agagccatgc ccataagatg gtgaagtggg
     6301 ggctgggcct ggatgacgag ggcagcagcc agggcgagcc acagtcaaag agcccccagg
     6361 agtcacgccg gctgagcatc cagcgctgca tccagtcgct ggtgcacgcg tgccagtgcc
     6421 gcaacgccaa ctgctcgctg ccatcctgcc agaagatgaa gcgggtggtg cagcacacca
     6481 agggctgcaa acgcaagacc aacgggggct gcccggtgtg caagcagctc atcgccctct
     6541 gctgctacca cgccaagcac tgccaagaaa acaaatgccc cgtgcccttc tgcctcaaca
     6601 tcaaacacaa gctccgccag cagcagatcc agcaccgcct gcagcaggcc cagctcatgc
     6661 gccggcggat ggccaccatg aacacccgca acgtgcctca gcagagtctg ccttctccta
     6721 cctcagcacc gcccgggacc cccacacagc agcccagcac accccagacg ccgcagcccc
     6781 ctgcccagcc ccaaccctca cccgtgagca tgtcaccagc tggcttcccc agcgtggccc
     6841 ggactcagcc ccccaccacg gtgtccacag ggaagcctac cagccaggtg ccggcccccc
     6901 cacccccggc ccagccccct cctgcagcgg tggaagcggc tcggcagatc gagcgtgagg
     6961 cccagcagca gcagcacctg taccgggtga acatcaacaa cagcatgccc ccaggacgca
     7021 cgggcatggg gaccccgggg agccagatgg cccccgtgag cctgaatgtg ccccgaccca
     7081 accaggtgag cgggcccgtc atgcccagca tgcctcccgg gcagtggcag caggcgcccc
     7141 ttccccagca gcagcccatg ccaggcttgc ccaggcctgt gatatccatg caggcccagg
     7201 cggccgtggc tgggccccgg atgcccagcg tgcagccacc caggagcatc tcacccagcg
     7261 ctctgcaaga cctgctgcgg accctgaagt cgcccagctc ccctcagcag caacagcagg
     7321 tgctgaacat tctcaaatca aacccgcagc taatggcagc tttcatcaaa cagcgcacag
     7381 ccaagtacgt ggccaatcag cccggcatgc agccccagcc tggcctccag tcccagcccg
     7441 gcatgcaacc ccagcctggc atgcaccagc agcccagcct gcagaacctg aatgccatgc
     7501 aggctggcgt gccgcggccc ggtgtgcctc cacagcagca ggcgatggga ggcctgaacc
     7561 cccagggcca ggccttgaac atcatgaacc caggacacaa ccccaacatg gcgagtatga
     7621 atccacagta ccgagaaatg ttacggaggc agctgctgca gcagcagcag caacagcagc
     7681 agcaacaaca gcagcaacag cagcagcagc aagggagtgc cggcatggct gggggcatgg
     7741 cggggcacgg ccagttccag cagcctcaag gacccggagg ctacccaccg gccatgcagc
     7801 agcagcagcg catgcagcag catctccccc tccagggcag ctccatgggc cagatggcgg
     7861 ctcagatggg acagcttggc cagatggggc agccggggct gggggcagac agcaccccca
     7921 acatccagca agccctgcag cagcggattc tgcagcaaca gcagatgaag cagcagattg
     7981 ggtccccagg ccagccgaac cccatgagcc cccagcaaca catgctctca ggacagccac
     8041 aggcctcgca tctccctggc cagcagatcg ccacgtccct tagtaaccag gtgcggtctc
     8101 cagcccctgt ccagtctcca cggccccagt cccagcctcc acattccagc ccgtcaccac
     8161 ggatacagcc ccagccttcg ccacaccacg tctcacccca gactggttcc ccccaccccg
     8221 gactcgcagt caccatggcc agctccatag atcagggaca cttggggaac cccgaacaga
     8281 gtgcaatgct cccccagctg aacaccccca gcaggagtgc gctgtccagc gaactgtccc
     8341 tggtcgggga caccacgggg gacacgctag agaagtttgt ggagggcttg gtttctctcg
     8401 gctcgagcgg cgtcttcaca ctcgaagatt tcgttgggga ctggcgacag acagccggct
     8461 acaacctgga ccaagtcctt gaacagggag gtgtgtccag tttgtttcag aatctcgggg
     8521 tgtccgtaac tccgatccaa aggattgtcc tgagcggtga aaatgggctg aagatcgaca
     8581 tccatgtcat catcccgtat gaaggtctga gcggcgacca aatgggccag atcgaaaaaa
     8641 tttttaaggt ggtgtaccct gtggatgatc atcactttaa ggtgatcctg cactatggca
     8701 cactggtaat cgacggggtt acgccgaaca tgatcgacta tttcggacgg ccgtatgaag
     8761 gcatcgccgt gttcgacggc aaaaagatca ctgtaacagg gaccctgtgg aacggcaaca
     8821 aaattatcga cgagcgcctg atcaaccccg acggctccct gctgttccga gtaaccatca
     8881 acggagtgac cggctggcgg ctgtgcgaac gcattctggc gtaaggccgc gactctagag
     8941 tcgacctgca ggcatgcaag ctgatccggc tgctaacaaa gcccgaaagg aagctgagtt
     9001 ggctgctgcc accgctgagc aataactagc ataacccctt ggggcggccg cttcgagcag
     9061 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
     9121 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
     9181 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gagatgtggg
     9241 aggttttttt aagcaagtaa aacctctaca aatgtggtaa aatcgaattt taacaaaata
     9301 ttaacgctta caatttcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     9361 accgcatacg cggatctgcg cagcaccatg gcctgaaata acctctgaaa gaggaacttg
     9421 gttaggtacc ttctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg
     9481 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     9541 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     9601 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     9661 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     9721 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     9781 cttttgcaaa aagcttaatt aactgttgac aattaatcat cggcatagta tatcggcata
     9841 gtataatacg acaaggtgag gaactaaacc caggaggcag atcatgattg aacaagatgg
     9901 attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     9961 acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
    10021 tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg
    10081 gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
    10141 agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
    10201 ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
    10261 tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
    10321 tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
    10381 gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt
    10441 gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
    10501 catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
    10561 tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
    10621 cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
    10681 gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgatggccg
    10741 caataaaata tctttatttt cattacatct gtgtgttggt tttttgtgtg aatcgatagc
    10801 gataaggatc cgctcatgcg atgataagct gtcaaacatg agaattacaa cttatatcgt
    10861 atggggctga cttcaggtgc tacatttgaa gagataaatt gcactgaaat ctagaaatat
    10921 tttatctgat taataagatg atcttcttga gatcgttttg gtctgcgcgt aatctcttgc
    10981 tctgaaaacg aaaaaaccgc cttgcagggc ggtttttcga aggttctctg agctaccaac
    11041 tctttgaacc gaggtaactg gcttggagga gcgcagtcac caaaacttgt cctttcagtt
    11101 tagccttaac cggcgcatga cttcaagact aactcctcta aatcaattac cagtggctgc
    11161 tgccagtggt gcttttgcat gtctttccgg gttggactca agacgatagt taccggataa
    11221 ggcgcagcgg tcggactgaa cggggggttc gtgcatacag tccagcttgg agcgaactgc
    11281 ctacccggaa ctgagtgtca ggcgtggaat gagacaaacg cggccataac agcggaatga
    11341 caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccgcca gggggaaacg
    11401 cctggtatct ttatagtcct gtcgggtttc gccaccactg atttgagcgt cagatttcgt
    11461 gatgcttgtc aggggggcgg agcctatgga aaaacggctt tgccgcggcc ctctcacttc
    11521 cctgttaagt atcttcctgg catcttccag gaaatctccg ccccgttcgt aagccatttc
    11581 cgctcgccgc agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaatatat
    11641 cctgtatcac atattctgct gacgcaccgg tgcagccttt tttctcctgc cacatgaagc
    11701 acttcactga caccctcatc agtgccaaca tagtaagcca gtatacactc cgctagcgct
    11761 gatgtccggc ggtgcttttg ccgttacgca ccaccccgtc atgagattac gcgttttagc
    11821 ggtgcgtaca attaagggat tatggtaaat ccacttactg tctgccctcg tagccatcga
    11881 gataaaccgc agtactccgg ccacgatgcg tccggcgtag aggatcgaga tct
//
