LOCUS       sequence-479591-        5716 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  5' AAVLINK plasmid for NUP155 expression .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5716)
  AUTHORS   Zhonghua Lu Lab
  TITLE     AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery 
            in Gene Therapy
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAVLINK-EF1alpha-5' NUP155
FEATURES             Location/Qualifiers
     source          1..5716
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     promoter        175..386
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             399..667
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             704..730
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     rep_origin      3194..3649
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3281..3300)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3491..3512
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3666..3685)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3785..3807
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3845..3863)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3931..4035
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4036..4104,4105..4896)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4254..4273)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5067..5655
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5556..5575
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtggatct gcgatcgctc cggtgcccgt cagtgggcag
      181 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg
      241 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
      301 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
      361 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac
      421 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc
      481 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga
      541 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc
      601 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt
      661 acagatccaa gctgtgaccg gcgcctaccg tacggccacc atgtaccctt atgacgtacc
      721 tgactatgct ggatctccgt cttctttgtt gggtgcggcg atgccggcct ctacatctgc
      781 cgcagccctg caggaagctc tggaaaatgc tggacggctc atcgaccgtc agttgcaaga
      841 ggaccgcatg tacccggacc tttccgagct gcttatggtg tctgccccaa ataatcccac
      901 cgtttctggc atgtcagata tggattatcc tttacaagga cctggtttgc tgtccgtacc
      961 caaccttcca gagatcagtt ccatccgaag agttcctctc ccacctgaac ttgttgagca
     1021 gtttggacat atgcagtgta attgcatgat gggtgtgttc cctcctatca gcagagcttg
     1081 gctcacaatt gacagtgata tattcatgtg gaactatgag gatggaggag accttgccta
     1141 ttttgatgga cttagtgaga ctattcttgc tgtggggctt gtgaagccaa aagcaggcat
     1201 ctttcaacct catgtgcgac acctcctggt tttggcgacc cctgtagaca tagtaattct
     1261 tggactcagc tatgctaatt tgcaaacagg ttctggagtt cttaatgata gtttgtctgg
     1321 tggaatgcag ttgcttccag atcctttata ttctcttcct actgataata cttacctttt
     1381 aacaataact tccactgata atggcagaat tttcttggct ggaaaggatg gctgtttata
     1441 tgaagtagcc taccaggctg aagcagggtg gtttagccaa agatgtagga aaataaacca
     1501 ctcaaagagc tcactttctt tccttgttcc ttccttgcta caattcacgt tctcagaaga
     1561 tgatcctatt cttcaaattg caattgataa ttctagaaat attttatata cacgatctga
     1621 gaaaggagta atacaggtgt atgatttggg acaagatgga caaggaatga gcagagttgc
     1681 ctctgtgtca cagaatgcca ttgtctctgc tgctggtaac attgctagga ccatcgatcg
     1741 ttctgttttt aaaccaattg tccaaatagc agtgattgaa aattctgaat cactggactg
     1801 tcagttattg gctgtcacac atgcaggtgt taggttatat tttagcactt gtccattcag
     1861 acagccatta gcacggccta atacactgac gctggttcat gtccgcttac ctcctggatt
     1921 ctcagcatct tcaaccgtgg aaaagccttc aaaagtacat agagctcttt atagtaaagg
     1981 tattctattg atggcagcct cagaaaatga ggataatgat attttatggt gtgtcaacca
     2041 tgatactttt cctttccaaa agccaatgat ggaaacccag atgacagctg gtgttgatgg
     2101 tcattcctgg gctctttctg cgatagatga attgaaagta gataaaataa ttacaccttt
     2161 aaacaaggat catattccaa taactgattc accagttgtt gtacagcagc acatgttacc
     2221 tccgaagaaa tttgttctcc tctcagcaca ggggagcctt atgtttcata aacttagacc
     2281 tgtagatcaa ctgaggcatc tacttgtgag taatgtggga ggagatggag aagagattga
     2341 aagattcttt aaattacatc aggaagacca ggcttgtgca acttgcctta ttcttgcttg
     2401 ctccactgct gcctgtgata gagaagtatc tgcctgggct actcgggctt tctttaggta
     2461 tggtggtgaa gcacagatga gatttccaac cactcttccg cctccaagta atgttggtcc
     2521 catcttgggg tctcctgtct attctagttc tcctgttcct agtggtagtc cctatccaaa
     2581 tccatccttt ttgggaacac cgtctcatgg tatacagcct cctgccatgt caactccagt
     2641 gtgtgctctg ggaaacccag caactcaggc cacaaatatg agttgtgtga ctggaccaga
     2701 gattgtgtac tctggaaaac acaatggtat ttgcatttac ttttctcgga tcatgggaaa
     2761 catttgggat gcaagcttag ttgtggagag aatattcaag agtggcaaca gagagatcac
     2821 tgcaattgaa agtagtgttc cctgccaact gctagagtca gtgctacaag aactaaaggt
     2881 aagtatcaag gttacaagac aggtttaagg agaccaataa gctattattc gtataggata
     2941 ctttatacga agttattaag ttatggagac cggtgtggca cgtgcggacc gagcggccgc
     3001 aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg
     3061 ccgggcgacc aaaggtcgcc cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg
     3121 gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca
     3181 aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     3241 cgcagcgtga ccgctacact tgccagcgcc ttagcgcccg ctcctttcgc tttcttccct
     3301 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     3361 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt
     3421 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     3481 ttctttaata gtggactctt gttccaaact ggaacaacac tcaactctat ctcgggctat
     3541 tcttttgatt tataagggat tttgccgatt tcggtctatt ggttaaaaaa tgagctgatt
     3601 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact
     3661 ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc
     3721 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
     3781 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga
     3841 aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag
     3901 acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     3961 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     4021 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     4081 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     4141 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     4201 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     4261 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     4321 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     4381 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     4441 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     4501 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     4561 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     4621 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     4681 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     4741 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     4801 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     4861 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     4921 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     4981 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     5041 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     5101 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     5161 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     5221 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     5281 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     5341 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     5401 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     5461 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     5521 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     5581 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     5641 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     5701 ccttttgctc acatgt
//
